LOCUS       NM_001394909            5168 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens latent transforming growth factor beta binding protein
            1 (LTBP1), transcript variant 10, mRNA.
ACCESSION   NM_001394909
VERSION     NM_001394909.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Jiang,X., Yin,S., Yin,X., Wang,Y., Fang,T., Yang,S., Bian,X.,
            Li,G., Xue,Y. and Zhang,L.
  TITLE     A prognostic marker LTBP1 is associated with epithelial mesenchymal
            transition and can promote the progression of gastric cancer
  JOURNAL   Funct Integr Genomics 24 (1), 30 (2024)
   PUBMED   38358412
  REMARK    GeneRIF: A prognostic marker LTBP1 is associated with epithelial
            mesenchymal transition and can promote the progression of gastric
            cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Wenk,D., Khan,S., Ignatchenko,V., May,T., Bernardini,M.Q. and
            Kislinger,T.
  TITLE     Targeted Mass Spectrometry of Longitudinal Patient Sera Reveals
            LTBP1 as a Potential Surveillance Biomarker for High-Grade Serous
            Ovarian Carcinoma
  JOURNAL   J Proteome Res 23 (2), 749-759 (2024)
   PUBMED   38266179
  REMARK    GeneRIF: Targeted Mass Spectrometry of Longitudinal Patient Sera
            Reveals LTBP1 as a Potential Surveillance Biomarker for High-Grade
            Serous Ovarian Carcinoma.
REFERENCE   3  (bases 1 to 5168)
  AUTHORS   Gu,H., Wang,W., Sun,C., Ding,L., Li,L., Shu,P. and Xu,J.
  TITLE     Immune suppressive signaling regulated by latent transforming
            growth factor beta binding protein 1 promotes metastasis in
            cervical cancer
  JOURNAL   Braz J Med Biol Res 55, e12206 (2023)
   PUBMED   36629522
  REMARK    GeneRIF: Immune suppressive signaling regulated by latent
            transforming growth factor beta binding protein 1 promotes
            metastasis in cervical cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5168)
  AUTHORS   Przyklenk,M., Georgieva,V.S., Metzen,F., Mostert,S., Kobbe,B.,
            Callewaert,B., Sengle,G., Brachvogel,B., Mecham,R.P., Paulsson,M.,
            Wagener,R., Koch,M. and Schiavinato,A.
  TITLE     LTBP1 promotes fibrillin incorporation into the extracellular
            matrix
  JOURNAL   Matrix Biol 110, 60-75 (2022)
   PUBMED   35452817
  REMARK    GeneRIF: LTBP1 promotes fibrillin incorporation into the
            extracellular matrix.
REFERENCE   5  (bases 1 to 5168)
  AUTHORS   Taipale,J., Saharinen,J., Hedman,K. and Keski-Oja,J.
  TITLE     Latent transforming growth factor-beta 1 and its binding protein
            are components of extracellular matrix microfibrils
  JOURNAL   J Histochem Cytochem 44 (8), 875-889 (1996)
   PUBMED   8756760
REFERENCE   6  (bases 1 to 5168)
  AUTHORS   Saharinen,J., Taipale,J. and Keski-Oja,J.
  TITLE     Association of the small latent transforming growth factor-beta
            with an eight cysteine repeat of its binding protein LTBP-1
  JOURNAL   EMBO J 15 (2), 245-253 (1996)
   PUBMED   8617200
REFERENCE   7  (bases 1 to 5168)
  AUTHORS   Olofsson,A., Ichijo,H., Moren,A., ten Dijke,P., Miyazono,K. and
            Heldin,C.H.
  TITLE     Efficient association of an amino-terminally extended form of human
            latent transforming growth factor-beta binding protein with the
            extracellular matrix
  JOURNAL   J Biol Chem 270 (52), 31294-31297 (1995)
   PUBMED   8537398
REFERENCE   8  (bases 1 to 5168)
  AUTHORS   Stenman,G., Sahlin,P., Olofsson,A., Geurts van Kessel,A. and
            Miyazono,K.
  TITLE     Assignment of the gene encoding the latent TGF-beta 1-binding
            protein (LTBP1) to human chromosome 2, region p12-->q22
  JOURNAL   Cytogenet Cell Genet 66 (2), 117-119 (1994)
   PUBMED   8287682
REFERENCE   9  (bases 1 to 5168)
  AUTHORS   Miyazono,K., Olofsson,A., Colosetti,P. and Heldin,C.H.
  TITLE     A role of the latent TGF-beta 1-binding protein in the assembly and
            secretion of TGF-beta 1
  JOURNAL   EMBO J 10 (5), 1091-1101 (1991)
   PUBMED   2022183
REFERENCE   10 (bases 1 to 5168)
  AUTHORS   Kanzaki,T., Olofsson,A., Moren,A., Wernstedt,C., Hellman,U.,
            Miyazono,K., Claesson-Welsh,L. and Heldin,C.H.
  TITLE     TGF-beta 1 binding protein: a component of the large latent complex
            of TGF-beta 1 with multiple repeat sequences
  JOURNAL   Cell 61 (6), 1051-1061 (1990)
   PUBMED   2350783
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019195.10, AC019127.10 and
            AC020594.6.
            
            Summary: The protein encoded by this gene belongs to the family of
            latent TGF-beta binding proteins (LTBPs). The secretion and
            activation of TGF-betas is regulated by their association with
            latency-associated proteins and with latent TGF-beta binding
            proteins. The product of this gene targets latent complexes of
            transforming growth factor beta to the extracellular matrix, where
            the latent cytokine is subsequently activated by several different
            mechanisms. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2278830.1,
                                           SRR14038196.1657076.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-365               AC019195.10        138996-139360       c
            366-590             AC019195.10        86876-87100         c
            591-865             AC019195.10        85105-85379         c
            866-968             AC019195.10        56302-56404         c
            969-1040            AC019195.10        51805-51876         c
            1041-1163           AC019195.10        30172-30294         c
            1164-1331           AC019195.10        21112-21279         c
            1332-1559           AC019195.10        16445-16672         c
            1560-1582           AC019195.10        14346-14368         c
            1583-1682           AC019195.10        11135-11234         c
            1683-1781           AC019195.10        10564-10662         c
            1782-1907           AC019127.10        3010-3135
            1908-2030           AC019127.10        3846-3968
            2031-2150           AC019127.10        8084-8203
            2151-2273           AC019127.10        21205-21327
            2274-2396           AC019127.10        28496-28618
            2397-2519           AC019127.10        29567-29689
            2520-2642           AC019127.10        37479-37601
            2643-2768           AC019127.10        43189-43314
            2769-2894           AC019127.10        70883-71008
            2895-3038           AC019127.10        75412-75555
            3039-3221           AC019127.10        88642-88824
            3222-3308           AC019127.10        89474-89560
            3309-3437           AC019127.10        91435-91563
            3438-3578           AC019127.10        92261-92401
            3579-3749           AC019127.10        93378-93548
            3750-3872           AC020594.6         14111-14233
            3873-4022           AC020594.6         22060-22209
            4023-5168           AC020594.6         23291-24436
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p22.3"
     gene            1..5168
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="latent transforming growth factor beta binding
                     protein 1"
                     /db_xref="GeneID:4052"
                     /db_xref="HGNC:HGNC:6714"
                     /db_xref="MIM:150390"
     exon            1..365
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..4204
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="isoform 10 precursor is encoded by transcript
                     variant 10; TGF-beta1-BP-1; transforming growth factor
                     beta-1-binding protein 1; LTBP-1"
                     /codon_start=1
                     /product="latent-transforming growth factor beta-binding
                     protein 1 isoform 10 precursor"
                     /protein_id="NP_001381838.1"
                     /db_xref="GeneID:4052"
                     /db_xref="HGNC:HGNC:6714"
                     /db_xref="MIM:150390"
                     /translation="MDTKLMCLLFFFSLPPLLVSNHTGRIKVVFTPSICKVTCTKGSC
                     QNSCEKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGK
                     LCQIPVHGASVPKLYQHSQQPGKALGTHVIHSTHTLPLTVTSQQGVKVKFPPNIVNIH
                     VKHPPEASVQIHQVSRIDGPTGQKTKEAQPGQSQVSYQGLPVQKTQTIHSTYSHQQVI
                     PHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKPS
                     YHGYNQMMECLPGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCTCKIGFGPDP
                     TFSSCVPDPPVISEEKGPCYRLVSSGRQCMHPLSVHLTKQLCCCSVGKAWGPHCEKCP
                     LPGTAAFKEICPGGMGYTVSGVHRRRPIHHHVGKGPVFVKPKNTQPVAKSTHPPPLPA
                     KEEPVEALTFSREHGPGVAEPEVATAPPEKEIPSLDQEKTKLEPGQPQLSPGISTIHL
                     HPQFPVVIEKTSPPVPVEVAPEASTSSASQVIAPTQVTDIDECTQVQHLCSQGRCENT
                     EGSFLCICPAGFMASEEGTNCIDVDECLRPDVCGEGHCVNTVGAFRCEYCDSGYRMTQ
                     RGRCEDIDECLNPSTCPDEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLEPNVCAN
                     GDCSNLEGSYMCSCHKGYTRTPDHKHCRDIDECQQGNLCVNGQCKNTEGSFRCTCGQG
                     YQLSAAKDQCEDIDECQHRHLCAHGQCRNTEGSFQCVCDQGYRASGLGDHCEDINECL
                     EDKSVCQRGDCINTAGSYDCTCPDGFQLDDNKTCQDINECEHPGLCGPQGECLNTEGS
                     FHCVCQQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQ
                     GCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRTSTDLDVDVD
                     QPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGVGWGDNCEIFPCPVLGTAEFT
                     EMCPKGKGFVPAGESSSEAGGENYKDADECLLFGQEICKNGFCLNTRPGYECYCKQGT
                     YYDPVKLQCFDMDECQDPSSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCIRPAESNE
                     QIEETDVYQDLCWEHLSDEYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPLKDSDD
                     YAQLCNIPVTGRRQPYGRDALVDFSEQYTPEADPYFIQDRFLNSFEELQAEECGILNG
                     CENGRCVRVQEGYTCDCFDGYHLDTAKMTCVDVNECDELNNRMSLCKNAKCINTDGSY
                     KCLCLPGYVPSDKPNYCTPLNTALNLEKDSDLE"
     exon            366..590
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            591..865
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            866..968
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1040
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1163
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1331
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1559
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1582
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1682
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1781
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1907
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2030
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2150
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2273
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2396
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2397..2519
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2642
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2768
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2894
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            2895..3038
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3221
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..3308
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3437
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3578
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3749
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3750..3872
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4022
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     exon            4023..5168
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4996..5001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="hexamer: AATAAA"
     polyA_site      5022
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="major polyA site"
     regulatory      5137..5142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
                     /note="hexamer: ATTAAA"
     polyA_site      5168
                     /gene="LTBP1"
                     /gene_synonym="ARCL2E"
ORIGIN      
        1 agagacacca ctgaatacag agcagcgagc actgaaggct tccctctttc cttaaacctg
       61 tcgggttgtg ggctctctct tttcccctct tgctcctttc ttttcttttt ttctgttttt
      121 ttaaaccttc caaggcaagt tcatggatac taagctgatg tgtttgttgt tctttttctc
      181 cctgcctccg ctcctagtga gtaaccacac tggccgcatc aaggtggtct ttactccgag
      241 catctgtaaa gtgacctgca ccaagggcag ctgtcagaac agctgtgaga aggggaacac
      301 caccactctc attagtgaga atggtcatgc tgccgacacc ctgacggcca cgaacttccg
      361 agtggtaatt tgccatcttc catgtatgaa tggtggccag tgcagttcaa gggacaaatg
      421 tcagtgccct ccaaatttca caggaaaact ttgtcagatc ccagtccatg gtgccagcgt
      481 gcctaaactt tatcagcatt cccagcagcc aggcaaggcg ttggggacgc atgtcatcca
      541 ttcaacacat accttgcctc tgaccgtgac tagccagcaa ggagtcaaag tgaaatttcc
      601 tcctaacata gtcaatatcc atgtgaaaca tcctcctgaa gcttccgtcc agatacatca
      661 ggtttcaaga attgatggcc caacaggcca gaagacaaaa gaagctcaac caggccaatc
      721 ccaagtctcg taccaagggc ttcctgtcca gaagacccag accatacatt ccacatactc
      781 ccaccagcag gtcattcctc acgtctaccc cgtggctgct aagacacagc ttggccggtg
      841 cttccaggaa accattgggt cacagtgtgg caaagcgctc cctggccttt caaagcaaga
      901 ggactgctgt ggaactgtgg gtacctcctg gggctttaac aaatgccaga aatgccccaa
      961 gaaaccatct tatcatggat acaaccaaat gatggaatgc ctaccgggtt ataagcgggt
     1021 taacaacacc ttttgccaag atattaatga atgtcagcta caaggtgtat gccctaatgg
     1081 tgagtgtttg aataccatgg gcagctatcg atgtacctgc aaaataggat ttgggccgga
     1141 tcctaccttt tcaagttgtg ttcctgatcc ccctgtgatc tcggaagaga aagggccctg
     1201 ttaccgactt gtcagttctg gaagacagtg tatgcaccct ctgtctgttc acctcaccaa
     1261 gcagctctgc tgttgtagtg tgggcaaggc ctggggccca cactgtgaga aatgtcccct
     1321 tccaggcaca gctgctttta aggaaatctg tcctggtgga atgggttata cggtttctgg
     1381 cgttcataga cgcaggccaa tccatcacca tgtaggtaaa ggacctgtat ttgtcaagcc
     1441 aaagaacact caacctgttg ctaaaagtac tcatcctcca cctctcccag ccaaggaaga
     1501 gccagtggag gccctgacct tctcccggga acacgggcca ggagtggcgg agccagaagt
     1561 ggcaactgca ccccctgaaa aggaaatacc ttcattggat caagagaaaa ccaaacttga
     1621 gcctggtcaa ccccagctgt ctccaggcat ttccactatt catctgcatc cacagtttcc
     1681 agtagtgatt gaaaaaacat cacctcctgt gcctgttgaa gtagctcctg aagcttctac
     1741 gtctagtgcc agccaagtga ttgctcctac tcaagtgaca gatattgatg agtgtactca
     1801 ggtccaacac ctctgctccc agggccgctg tgaaaacacc gagggaagtt tcttgtgcat
     1861 ttgcccagca ggatttatgg ccagtgagga gggtactaac tgcatagatg ttgacgaatg
     1921 cctgaggccg gacgtctgtg gggaggggca ctgtgtcaat actgtggggg ccttccggtg
     1981 tgaatactgt gacagcgggt accgcatgac tcagagaggc cgttgtgagg atattgatga
     2041 atgtttgaat ccaagcactt gtccagatga gcagtgtgtg aattctcctg gatcttacca
     2101 gtgcgttccc tgcacagaag gattccgagg ctggaatgga cagtgccttg atgtggacga
     2161 gtgcctggaa ccaaacgtct gcgcaaatgg tgattgttcc aaccttgaag gctcctacat
     2221 gtgttcatgc cacaaaggct atacccggac tccggaccac aagcactgta gagatattga
     2281 tgaatgtcag caagggaatc tatgtgtaaa cgggcagtgc aaaaataccg agggctcctt
     2341 caggtgcacc tgtggacagg ggtaccagct gtcggcagct aaagaccagt gtgaagacat
     2401 tgatgaatgc cagcaccgtc atctctgtgc tcatgggcag tgcaggaaca ctgagggctc
     2461 ttttcaatgt gtgtgtgacc agggttacag agcatctggg cttggagacc actgtgaaga
     2521 tatcaatgaa tgcttggagg acaagagtgt ttgccagaga ggagactgca ttaatactgc
     2581 agggtcctat gattgtactt gtccggatgg atttcagcta gatgacaata aaacatgtca
     2641 agatattaat gaatgtgaac atccagggct ctgtggtccg caaggggagt gcctaaacac
     2701 agagggttct ttccattgtg tctgccagca gggtttctca atctctgcag atggccgtac
     2761 gtgtgaagat attgatgaat gtgtaaacaa cactgtttgt gacagtcacg ggttttgtga
     2821 caatacagct ggctccttcc gctgcctctg ttatcagggc tttcaagccc cacaggatgg
     2881 gcaagggtgt gtggatgtga atgaatgtga actgctcagt ggggtgtgtg gtgaagcctt
     2941 ctgtgaaaac gtggaagggt ccttcctgtg cgtgtgtgct gatgaaaacc aagagtacag
     3001 ccccatgact gggcagtgcc gctcccggac ctccacagat ttagatgtag atgtagatca
     3061 acccaaagaa gaaaagaaag aatgctacta taatctcaat gacgccagtc tctgtgataa
     3121 tgtgttggcc cccaatgtca cgaaacaaga atgctgctgt acatcaggcg tgggatgggg
     3181 agataactgc gaaatcttcc cctgcccggt cttgggaact gctgagttca ctgaaatgtg
     3241 tcccaaaggg aaaggttttg tgcctgctgg agaatcatct tctgaagctg gtggtgagaa
     3301 ctataaagat gcagatgaat gcctactttt tggacaagaa atctgcaaaa atggtttctg
     3361 tttgaacact cggcctgggt atgaatgcta ctgtaagcaa gggacgtact atgatcctgt
     3421 gaaactgcag tgctttgata tggatgaatg tcaagacccc agtagttgta ttgatggcca
     3481 gtgtgttaat acagagggct cttacaactg cttctgtact caccccatgg tcctggatgc
     3541 gtcagaaaaa agatgtatac gaccggctga gtcaaacgaa caaatagaag aaactgatgt
     3601 ctaccaagat ttgtgctggg aacatctgag tgatgaatac gtgtgtagcc ggcctcttgt
     3661 gggcaagcag acaacgtaca ctgagtgctg ctgtctgtat ggagaggcct ggggcatgca
     3721 gtgtgccctc tgccccctga aggattcaga tgactatgct cagctgtgta acatccccgt
     3781 gacgggacgc cggcagccat atggacggga cgccttggtt gacttcagtg aacagtatac
     3841 tccagaagcc gatccctact tcatccaaga ccgttttcta aatagctttg aggagttaca
     3901 ggctgaggaa tgcggcatcc tcaatggatg tgaaaatggt cgctgtgtga gggtccagga
     3961 aggttacacc tgcgattgct ttgatgggta tcacttggat acggccaaga tgacctgtgt
     4021 cgatgtaaat gaatgcgatg agttgaacaa ccggatgtct ctctgcaaga atgccaagtg
     4081 cattaacacc gatggttcct acaagtgttt gtgtctgcca ggctacgtgc cttctgacaa
     4141 gccaaactac tgcactccgt tgaataccgc cttgaattta gagaaagaca gtgacctgga
     4201 gtgaaacaga atctacataa cctaagccca tatactctgc actgtgtaaa ggaaaaggga
     4261 gaaatgtatt atacttgaga cattgcacct accccggaag gctggaaata cagaaacagc
     4321 atggaattgc aagtcctctg aagacaatga gaggatttag gatgagcccg ataggtgtgg
     4381 cagaccaaat ggacatttct ctaaaaaacc agtatatata gtctgttcat atgtaaaatt
     4441 caatggaaga gaggtggaac agtgctgtta ttttaaacag aaggttgtat tattatgttg
     4501 ttttgttttt ttactattgc ttgattaaat ttggcattta aatagtggtg gaaatatttt
     4561 atataatttt cattttttgg ttgtgcagtt ccttggctac tgtttttctt ttacttcagt
     4621 tttttaaaaa tctcaaatga aaaagtcttc gatacaatat tgttaagctg tattataagt
     4681 attgttacac agggttatgc aattcccggc ctggagcatt tttgaaattc aaattgtctg
     4741 tcctgtggag caggcagtga ttttgttcca aaactttgta tacacatttg gagaaaagta
     4801 ctttatattt tcagtgtttt gtctgatttt aatgtccgtt cttagccaag ctgctagcag
     4861 gtgttaattg gatccctttc cttcactgaa atggaagagt ttataagctt acgttagtat
     4921 tgtaatatgt aaagtaagcc caacaaaaat ttttaaaaat ttgatgatcc ccaatatatc
     4981 taccattgta tgttaaataa atcaccattt ttgtagaaaa aattctacct gagagtaatt
     5041 gtcaatgagt acatgtgtat aagttgtatc ccactctccc cacttttatc ttttccagtg
     5101 gtcttctgtt aatgtagtgt cttttacaag ttaatcatta aatttgttag atcttgttat
     5161 gggctaaa
//
