LOCUS       NM_014463               3359 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens LSM3 homolog, U6 small nuclear RNA and mRNA
            degradation associated (LSM3), mRNA.
ACCESSION   NM_014463
VERSION     NM_014463.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3359)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3359)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3359)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 3359)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   7  (bases 1 to 3359)
  AUTHORS   Eystathioy,T., Peebles,C.L., Hamel,J.C., Vaughn,J.H. and Chan,E.K.
  TITLE     Autoantibody to hLSm4 and the heptameric LSm complex in anti-Sm
            sera
  JOURNAL   Arthritis Rheum 46 (3), 726-734 (2002)
   PUBMED   11920408
REFERENCE   8  (bases 1 to 3359)
  AUTHORS   Friesen,W.J. and Dreyfuss,G.
  TITLE     Specific sequences of the Sm and Sm-like (Lsm) proteins mediate
            their interaction with the spinal muscular atrophy disease gene
            product (SMN)
  JOURNAL   J Biol Chem 275 (34), 26370-26375 (2000)
   PUBMED   10851237
REFERENCE   9  (bases 1 to 3359)
  AUTHORS   Achsel,T., Brahms,H., Kastner,B., Bachi,A., Wilm,M. and Luhrmann,R.
  TITLE     A doughnut-shaped heteromer of human Sm-like proteins binds to the
            3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in
            vitro
  JOURNAL   EMBO J 18 (20), 5789-5802 (1999)
   PUBMED   10523320
REFERENCE   10 (bases 1 to 3359)
  AUTHORS   Salgado-Garrido,J., Bragado-Nilsson,E., Kandels-Lewis,S. and
            Seraphin,B.
  TITLE     Sm and Sm-like proteins assemble in two related complexes of deep
            evolutionary origin
  JOURNAL   EMBO J 18 (12), 3451-3462 (1999)
   PUBMED   10369684
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB499707.1, AF182418.1, DB289601.1 and AC093495.2.
            
            On Nov 23, 2018 this sequence version replaced NM_014463.2.
            
            Summary: Sm-like proteins were identified in a variety of organisms
            based on sequence homology with the Sm protein family (see SNRPD2;
            MIM 601061). Sm-like proteins contain the Sm sequence motif, which
            consists of 2 regions separated by a linker of variable length that
            folds as a loop. The Sm-like proteins are thought to form a stable
            heteromer present in tri-snRNP particles, which are important for
            pre-mRNA splicing.[supplied by OMIM, Apr 2004].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM693722.1, SRR1660803.47170.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306024.4/ ENSP00000302160.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                DB499707.1         92-107
            17-592              AF182418.1         1-576
            593-606             DB289601.1         555-568
            607-3359            AC093495.2         59019-61771         c
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..3359
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /note="LSM3 homolog, U6 small nuclear RNA and mRNA
                     degradation associated"
                     /db_xref="GeneID:27258"
                     /db_xref="HGNC:HGNC:17874"
                     /db_xref="MIM:607283"
     exon            1..65
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..353
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /note="LSM3 homolog, U6 small nuclear RNA associated; LSM3
                     U6 small nuclear RNA and mRNA degradation associated"
                     /codon_start=1
                     /product="U6 snRNA-associated Sm-like protein LSm3"
                     /protein_id="NP_055278.1"
                     /db_xref="CCDS:CCDS2619.1"
                     /db_xref="GeneID:27258"
                     /db_xref="HGNC:HGNC:17874"
                     /db_xref="MIM:607283"
                     /translation="MADDVDQQQTTNTVEEPLDLIRLSLDERIYVKMRNDRELRGRLH
                     AYDQHLNMILGDVEETVTTIEIDEETYEEIYKSTKRNIPMLFVRGDGVVLVAPPLRVG
                     "
     exon            66..176
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /inference="alignment:Splign:2.1.0"
     exon            177..272
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /inference="alignment:Splign:2.1.0"
     exon            273..3359
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      558..563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /note="hexamer: AATAAA"
     polyA_site      584
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /note="major polyA site"
     polyA_site      649
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
     regulatory      3322..3327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
                     /note="hexamer: AATAAA"
     polyA_site      3359
                     /gene="LSM3"
                     /gene_synonym="SMX4; USS2; YLR438C"
ORIGIN      
        1 cttgtgttct cgcgagaggc gggaaagggc gcagggtttg aaacatggcg gacgacgtag
       61 accagcaaca aactaccaac actgtagagg agcccctgga tcttatcagg ctcagcctag
      121 atgagcgaat ttatgtgaaa atgagaaatg accgagagct tcgaggcaga ttacatgctt
      181 atgatcaaca tttaaatatg atcttgggag atgtggaaga aactgtgact actatagaaa
      241 ttgatgaaga aacatatgaa gagatatata aatcaacgaa acggaatatt ccaatgctct
      301 ttgtccgggg agatggcgtt gtcctggttg cccctccact gagagttggc tgaaacaaag
      361 aatttgtcct gtatggaaaa cgggagactt tgtacagtgg cctctctaaa agtacaaaac
      421 attcataaga gaaacctgca tacattttga tattaagaaa taattccggg gattcttcca
      481 ctcctgaaat gagttgattt gcagataact cacaacttct taagctaaat ggtattttca
      541 tttttctcaa gctctccaat aaatatgacc accaagatgc agaactcttt caggacttct
      601 tttgctccat tattctcaca gatacttact gatttttctt ttctttaaat tggtgtttcc
      661 ttgtagattt ttttaagtgc ggtcttctgt cttgtgaatt atttaaagta agcatgtctt
      721 accttcactt agcacacatt tgcagtgcac ctactgtgtt tgaggcacca ggtttgagtg
      781 ctgggaacca gagttttatc aaaatgcttt ctgggccaga catggtggct catgcctgta
      841 atcccagcac tttgggaggc caaggtgggt ggatcacctg aggtcaggcg tttgagacca
      901 gcctggccaa catggtgaaa ccctttctct actgaaaata taaaaattag ccaggcgtgg
      961 tggcgcatac ttgtaatccc agctactcag gaggctgagg cagtagacgc ttgaacccag
     1021 gaggcagagg ttgcagtgag ccaagatcac accattgcac ttcaacctgg tcaacagagc
     1081 gagactccgt gtcaaaaaaa aaaaaaaaaa agttttctgc cctcaagtct tctggggagg
     1141 cagatgttta aaccaacaat tgcaggcagt aagattagtg ctgatagaat catgcacatg
     1201 caggatgcca taggagcatg gggaagagca cccaaatatg ggagaaaggg gcagagggac
     1261 attgtaaaca tcaaccctgc agtagaagac agagcaaagc atgatttctc ttttctctgg
     1321 cctgatttaa ttctaaggtc agacgttcag cttgatcata acctgtttat aattatgact
     1381 cagaccattt ttccactctt ggatcattgg ccattaatga tgaggggtct caaacaacat
     1441 gtctcagtga cacagtaaag cagaaattgg ctgaaagcaa agctgttttt atttctagct
     1501 tcagtcagca cataatgctt aaaacctaaa gaggaaagta aaagcgtgtt acaatctctc
     1561 aaactttatc aagacagggc ttagaacctg acatcctccg ctatggtcat tccattttga
     1621 aatgtagaaa atccaacttg tggatggttt gtctgtaaat gagctgaatg tgtgtgcgtg
     1681 ttcattatca agcacgatat gctcacaaat gcacagtact ttaagccttg tgcaagaaag
     1741 atgctttcag taaacatatt taaaaggttt tattttcctt actaatggta catgcccctg
     1801 caagttcatg gagaagagaa aaacaagtgt gggtgagctg atggaggctt catcttaggt
     1861 gaggaggcag gagagacacc ctcccttcac ttgtgccatc cccacgcttt gtccttaagc
     1921 tgtagtggtc ccaggagata acctgtttac agcagcccat tcagtattga aaaatgaact
     1981 ccctcctgca gaatgttaga agaaacaacc tcaaagacct caggccgcct gtggtcatta
     2041 gtatgtggca gcaagattga acctgcctgc ctcctacctg tgtgagcctg tggtgtgggt
     2101 gtggtaccaa ggccctggct ctgggcagtc tgttggctcc tggtgttcac taaggcgtgg
     2161 ggtgacaggc catctggcct cgctctgcca gtctgcagtt ctccccagca ctgcattcat
     2221 ttttagtggt ttctgatagt cacactgaat tcctggacac tttcttctct tcattcttag
     2281 ttatctttct cctttttctc ctggaactga tagtttacac taaaactttt ggctgtgagg
     2341 acttgattat acacatttct aaaacccatg aaccttaata tctgacactg ttactctcat
     2401 atgcacacac aactaaaaca taccaaccta cttcatttcc tagctgctaa gatacaaggg
     2461 gagggacaat aaaaggttaa ctgcagaata gccacctatg tgtcaggaaa tggagggagg
     2521 ggaggcaatg tgtgagtcct tttggagctg ggactcaatc cacaacagag acagctcaag
     2581 cagggccctg ccatggtcca cactgaggtt tcctctgcag tggtgctgtc cactgagggg
     2641 ttatggtgtg agggcccctg ggagccacgc cttggacttc tgagtctgca gagcactcgg
     2701 ctccagccag ctggatggca ggtggtcagt ggagctggtg actggtggtt tctgcaatga
     2761 ggtgtcagat tagcctaagg gaagcctctt cagcaagtat catgcctgga tcccggtctt
     2821 ggttgtttta tgagaggccg atatgaattg ccattgcctc ccacaaagaa aaatggacaa
     2881 aatatgggac cacatctttg agatgaaatg tgcccaaaat gaaaagtaac ttgagtatcc
     2941 caagttaaac ttaatcatta gctgcaaatg gacgattttc aaatttcaaa tagtgatttt
     3001 tgaagatttg cccaaactag agtactatcc ctcttcacac taagaggcag aaagtggttt
     3061 cacacaccct tttatttaat cagctaaagt gggtgcggtg acacacacct gtaaactcaa
     3121 ccactaagga gactgaggcg ggaggattac ttgagcccag gagttcgaga tcagcctgag
     3181 caacataaca agaccctgtc tcaaataata cttagctatt acccatattg cttttagcct
     3241 gctgccagga ggggagaaat gttattgttt gaacagatgt cagatgtgag taatgctcag
     3301 ctgtgttcaa tgtagaaaat taataaaagt tccaggagtc atcctggcta gatcaagaa
//
