LOCUS       NM_018385               3283 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens large 60S subunit nuclear export GTPase 1 (LSG1),
            mRNA.
ACCESSION   NM_018385
VERSION     NM_018385.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Sutjita,P., Musalgaonkar,S., Recchia-Rife,J., Huang,L., Xhemalce,B.
            and Johnson,A.W.
  TITLE     The Ribosome Assembly Factor LSG1 Interacts with Vesicle-Associated
            Membrane Protein-Associated Proteins (VAPs)
  JOURNAL   Mol Cell Biol 44 (9), 345-357 (2024)
   PUBMED   39133101
  REMARK    GeneRIF: The Ribosome Assembly Factor LSG1 Interacts with
            Vesicle-Associated Membrane Protein-Associated Proteins (VAPs).
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Pantazi,A., Quintanilla,A., Hari,P., Tarrats,N., Parasyraki,E.,
            Dix,F.L., Patel,J., Chandra,T., Acosta,J.C. and Finch,A.J.
  TITLE     Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes
            endoplasmic reticular disruption and cellular senescence
  JOURNAL   Aging Cell 18 (4), e12981 (2019)
   PUBMED   31148378
  REMARK    GeneRIF: Targeting of LSG1 resulted in amplification of the
            cholesterol/endoplasmic reticular signature and restoration of a
            robust cellular senescence response in transformed cells,
            suggesting potential therapeutic uses of LSG1 inhibition.
REFERENCE   3  (bases 1 to 3283)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 3283)
  AUTHORS   Reynaud,E.G., Andrade,M.A., Bonneau,F., Ly,T.B., Knop,M.,
            Scheffzek,K. and Pepperkok,R.
  TITLE     Human Lsg1 defines a family of essential GTPases that correlates
            with the evolution of compartmentalization
  JOURNAL   BMC Biol 3, 21 (2005)
   PUBMED   16209721
  REMARK    GeneRIF: hLsg1 is an essential GTPase predominantly located in the
            endoplasmic reticulum and, in some cells, in Cajal bodies in the
            nucleus.
            Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA414880.1, BC040119.1,
            BC068500.1, AW748476.1, AC046143.20 and BQ009975.1.
            
            On Nov 22, 2018 this sequence version replaced NM_018385.2.
            
            Summary: This gene encodes a protein related to the yeast large
            subunit GTPase 1. The encoded protein is necessary for cell
            viability and may localize in the endoplasmic reticulum, nucleus
            and cytoplasm.[provided by RefSeq, Feb 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068500.1, AL136897.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265245.10/ ENSP00000265245.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DA414880.1         261-289
            30-1967             BC040119.1         1-1938
            1968-2081           BC068500.1         1968-2081
            2082-2632           AW748476.1         46-596              c
            2633-3035           AC046143.20        127044-127446       c
            3036-3283           BQ009975.1         21-268              c
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..3283
                     /gene="LSG1"
                     /note="large 60S subunit nuclear export GTPase 1"
                     /db_xref="GeneID:55341"
                     /db_xref="HGNC:HGNC:25652"
                     /db_xref="MIM:610780"
     exon            1..128
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..2006
                     /gene="LSG1"
                     /note="hLsg1"
                     /codon_start=1
                     /product="large subunit GTPase 1 homolog"
                     /protein_id="NP_060855.2"
                     /db_xref="CCDS:CCDS33922.1"
                     /db_xref="GeneID:55341"
                     /db_xref="HGNC:HGNC:25652"
                     /db_xref="MIM:610780"
                     /translation="MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYD
                     WGRLNLQSVTEQSSLDDFLATAELAGTEFVAEKLNIKFVPAEARTGLLSFEESQRIKK
                     LHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWRRQLVRLEEEQKLILTPFERN
                     LDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVILINKADLLT
                     AEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDRQSNTTKFGHSSFDQA
                     EISHSESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQ
                     DWKESSTADSEARSRKTPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLV
                     GYPNVGKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVSTK
                     AEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINIITPREDEDPHRPPTSEE
                     LLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQHQHQRLL
                     ENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTA
                     STASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM"
     exon            129..255
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            256..376
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            377..463
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            464..550
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            551..611
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            612..788
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            789..1202
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1304
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1448
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1572
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1652
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1826
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..3283
                     /gene="LSG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3261..3266
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LSG1"
                     /note="hexamer: ATTAAA"
     polyA_site      3283
                     /gene="LSG1"
                     /note="major polyA site"
ORIGIN      
        1 gggaagcttc gtccagcggt cgtgttgcca tgggccggag gagagccccg gccggtgggt
       61 cgctgggacg ggcccttatg cgccatcaga ctcagcggag ccgaagccat cgtcacactg
      121 actcctggtt gcacacaagt gaactcaatg atggctatga ttggggtcgt cttaatcttc
      181 agtcagtgac tgaacagagc tcccttgatg acttccttgc tactgcagaa cttgcaggaa
      241 cagagtttgt agctgagaaa cttaatatta agtttgtgcc tgctgaggct agaactggac
      301 tactgtcttt cgaggagagc cagagaatta agaagctcca tgaagaaaac aaacagttct
      361 tgtgtatacc gaggagacca aactggaacc aaaatactac cccagaagaa ctcaaacaag
      421 cagagaaaga taactttcta gaatggagac gtcagcttgt ccggctagaa gaggaacaga
      481 agctgatatt gactccattt gaacgaaatt tggacttttg gcgccagctc tggagagtca
      541 ttgagagaag tgatattgtg gtccagatag tagatgctcg aaacccactc ctgtttagat
      601 gtgaggattt ggaatgttat gtgaaagaaa tggatgccaa taaggagaac gtcattctga
      661 tcaacaaggc agacttgctg actgctgagc agcggagtgc ctgggccatg tacttcgaaa
      721 aagaagatgt gaaggttatt ttctggtcag ctttggccgg agccattccc ctgaatggtg
      781 actctgagga agaggcaaac agagatgata gacaaagcaa cacaaccaag tttggacatt
      841 ccagtttcga ccaggctgaa atttcccaca gtgaatccga acatctccca gctagggatt
      901 ctccttcact tagtgaaaat cccacaacgg atgaagatga cagtgagtat gaggactgtc
      961 cagaggagga ggaagacgac tggcagacgt gctcagaaga agacggtccc aaggaagagg
     1021 actgcagcca ggactggaag gaaagctcta ctgcagattc tgaggctcgg agcaggaaaa
     1081 ccccacagaa gaggcagata cacaatttta gccatctggt atccaagcag gagttactgg
     1141 agctctttaa ggagctacac actgggagaa aggtgaaaga tgggcaactt acggtcggac
     1201 tggtgggcta ccctaatgtt ggtaagagtt caacaatcaa caccatcatg ggcaacaaga
     1261 aagtatctgt gtctgccaca cctggtcaca caaagcactt tcagactctc tatgtggagc
     1321 ctggcctctg cctgtgtgac tgtcctggct tggtgatgcc atcttttgtg tctaccaagg
     1381 cagaaatgac ttgcagcgga atcctcccaa ttgatcagat gagagatcat gttcctcctg
     1441 tatcactagt ttgccagaat attccaagac atgttttaga agctacctat ggcattaaca
     1501 tcataacgcc tagagaggat gaagatcccc accgacctcc aacatcggaa gaactgttga
     1561 cagcttatgg atacatgcga ggattcatga cagcgcatgg acagccagac cagcctcgat
     1621 ctgcgcgcta catcctgaag gactatgtca gtggtaagct gctgtactgc catcctcctc
     1681 ctggaagaga tcctgtaact tttcagcatc aacaccagcg actcctagag aacaaaatga
     1741 acagtgatga aataaaaatg cagctaggca gaaataaaaa agcaaagcag attgaaaata
     1801 tcgttgacaa aacttttttc catcaagaga atgtgagggc tttgaccaaa ggagtccagg
     1861 ctgtgatggg ttacaagccc gggagtggtg tagtgactgc atccactgcg agctctgaga
     1921 acggggcggg gaagccctgg aaaaaacatg gcaacagaaa taaaaaagaa aaaagtcgta
     1981 gactctacaa gcacctggat atgtgaggtt gggctgcaac agaaatgtca tctgcattgt
     2041 gcagatggaa aagagcagaa gctgcctgtt gcctgtggaa ccgtcccaag acactagcac
     2101 tgtagaacgg gccctgctct tgcagagcac ggctgcaccc aacagtctcc atgtcaagac
     2161 caagggcctc ctggaaacac caactctgac aaaaaggagt catctgggag cccgagaatc
     2221 ctactcctgg ccgggcacag tggctcacgc accaacatgg agaaaccccg tctctactaa
     2281 aaatacaaaa aaattagcca ggcgtggtgg cgcgcacctg taatcccagc tactcgggag
     2341 gctgaggcag gagaatcact tgaaccaggg aggcagagtt tgcagtgaat ggagattgcg
     2401 ccgctgcact ccagcctggg cgacagagtg agactgcatc acaagaaaaa aaatttgcaa
     2461 gggatggttc acgagacaca tttgggacga aggtgaaaga gaaattcccc attctgagtg
     2521 tcctagttgg gttcctccga ctctaaacaa gggacttggg ttcagttagt gtacagcggg
     2581 ggctcacgtc cactaaggaa catgtagaat gtaaccaccg ggtgacaggg aagctgcggt
     2641 atttactacc tagcccccat cttcactggt tattccactt atttaaaatg tccagaataa
     2701 gcaaatctcc atatagagga agtagattag tggttgcttc gggatgggag gaatgggaag
     2761 attgaggtct ttcttttgca gtgataaaaa tgtcctaaaa ttgactgtag cgatggtcac
     2821 acaactctga atatgcttaa gaccattgaa ttacacactt tacgttggtg aattgtatgg
     2881 tatgtaaatt atagttcaat aacatagtta caaaagataa tcaaaagcat gaaagcactg
     2941 ttgatgtggt ttggatctgt gtcctcaccg agtctcatgt tgaaatgtaa gccccctggt
     3001 gggaggcgat gggattatgg ggcagagtcc tcacgaacgg tttagcacca cccgctcagt
     3061 gctgttctcc tgatattgag tcctcatcac atctggttgc ttcaaagtgt gtggtgcctc
     3121 ccctctgtct ccctcctgct ctggccatat aagatgtgcc tgcttctcct tcgccttcta
     3181 acatgattgt aagtttcctg aggcctccct agaagcaaaa gctgctgtgc ttcctgtacc
     3241 atctacagga ccgtgagcca attaaacctc ttttctttat aaa
//
