LOCUS       NM_152892               2160 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens leucine rich repeats and WD repeat domain containing 1
            (LRWD1), transcript variant 1, mRNA.
ACCESSION   NM_152892
VERSION     NM_152892.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Lin,Y.H., Su,C.H., Chen,H.M., Wu,M.S., Pan,H.A., Chang,C.N.,
            Cheng,Y.S., Chang,W.T., Chiu,C.C. and Teng,Y.N.
  TITLE     MicroRNA-320a enhances LRWD1 expression through the
            AGO2/FXR1-dependent pathway to affect cell behaviors and the
            oxidative stress response in human testicular embryonic carcinoma
            cells
  JOURNAL   Aging (Albany NY) 16 (4), 3973-3988 (2024)
   PUBMED   38385979
  REMARK    GeneRIF: MicroRNA-320a enhances LRWD1 expression through the
            AGO2/FXR1-dependent pathway to affect cell behaviors and the
            oxidative stress response in human testicular embryonic carcinoma
            cells.
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Hung,J.H., Wee,S.K., Omar,H.A., Su,C.H., Chen,H.Y., Chen,P.S.,
            Chiu,C.C., Wu,M.S. and Teng,Y.N.
  TITLE     Nuclear factor erythroid-2-related factor regulates LRWD1
            expression and cellular adaptation to oxidative stress in human
            embryonal carcinoma cells
  JOURNAL   Biochimie 148, 99-106 (2018)
   PUBMED   29544732
  REMARK    GeneRIF: Nuclear factor erythroid-2-related factor regulates LRWD1
            expression and cellular adaptation to oxidative stress in human
            embryonal carcinoma cells
REFERENCE   3  (bases 1 to 2160)
  AUTHORS   Wang,C.Y., Hong,Y.H., Syu,J.S., Tsai,Y.C., Liu,X.Y., Chen,T.Y.,
            Su,Y.M., Kuo,P.L., Lin,Y.M. and Teng,Y.N.
  TITLE     LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle
            Progression in the Human Testicular Embryonic Carcinoma Cells
  JOURNAL   J Cell Biochem 119 (1), 314-326 (2018)
   PUBMED   28569402
  REMARK    GeneRIF: a novel function of LRWD1 in controlling microtubule
            nucleation and cell cycle progression in the human testicular
            embryonic carcinoma cells.
REFERENCE   4  (bases 1 to 2160)
  AUTHORS   He,T., Surdez,D., Rantala,J.K., Haapa-Paananen,S., Ban,J.,
            Kauer,M., Tomazou,E., Fey,V., Alonso,J., Kovar,H., Delattre,O. and
            Iljin,K.
  TITLE     High-throughput RNAi screen in Ewing sarcoma cells identifies
            leucine rich repeats and WD repeat domain containing 1 (LRWD1) as a
            regulator of EWS-FLI1 driven cell viability
  JOURNAL   Gene 596, 137-146 (2017)
   PUBMED   27760381
  REMARK    GeneRIF: LRWD1 is a novel regulator of EWS-FLI1 driven cell
            proliferation in Ewing sarcoma cells. EWS-FLI1 regulates LRWD1
            expression and LRWD1 may contribute to EWS-FLI1 mediated
            transcriptional repression.
REFERENCE   5  (bases 1 to 2160)
  AUTHORS   Giri,S., Aggarwal,V., Pontis,J., Shen,Z., Chakraborty,A., Khan,A.,
            Mizzen,C., Prasanth,K.V., Ait-Si-Ali,S., Ha,T. and Prasanth,S.G.
  TITLE     The preRC protein ORCA organizes heterochromatin by assembling
            histone H3 lysine 9 methyltransferases on chromatin
  JOURNAL   Elife 4, e06496 (2015)
   PUBMED   25922909
  REMARK    GeneRIF: Data show that ORCA acts as a scaffold for the
            establishment of H3K9 KMT complex.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2160)
  AUTHORS   Teng,Y.N., Liao,M.H., Lin,Y.B., Kuo,P.L. and Kuo,T.Y.
  TITLE     Expression of lrwd1 in mouse testis and its centrosomal
            localization
  JOURNAL   Int J Androl 33 (6), 832-840 (2010)
   PUBMED   20180869
REFERENCE   7  (bases 1 to 2160)
  AUTHORS   Vermeulen,M., Eberl,H.C., Matarese,F., Marks,H., Denissov,S.,
            Butter,F., Lee,K.K., Olsen,J.V., Hyman,A.A., Stunnenberg,H.G. and
            Mann,M.
  TITLE     Quantitative interaction proteomics and genome-wide profiling of
            epigenetic histone marks and their readers
  JOURNAL   Cell 142 (6), 967-980 (2010)
   PUBMED   20850016
REFERENCE   8  (bases 1 to 2160)
  AUTHORS   Ohta,S., Bukowski-Wills,J.C., Sanchez-Pulido,L., Alves Fde,L.,
            Wood,L., Chen,Z.A., Platani,M., Fischer,L., Hudson,D.F.,
            Ponting,C.P., Fukagawa,T., Earnshaw,W.C. and Rappsilber,J.
  TITLE     The protein composition of mitotic chromosomes determined using
            multiclassifier combinatorial proteomics
  JOURNAL   Cell 142 (5), 810-821 (2010)
   PUBMED   20813266
REFERENCE   9  (bases 1 to 2160)
  AUTHORS   Tegha-Dunghu,J., Neumann,B., Reber,S., Krause,R., Erfle,H.,
            Walter,T., Held,M., Rogers,P., Hupfeld,K., Ruppert,T., Ellenberg,J.
            and Gruss,O.J.
  TITLE     EML3 is a nuclear microtubule-binding protein required for the
            correct alignment of chromosomes in metaphase
  JOURNAL   J Cell Sci 121 (Pt 10), 1718-1726 (2008)
   PUBMED   18445686
REFERENCE   10 (bases 1 to 2160)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC030547.1 and BC009436.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152892.2.
            
            Summary: The protein encoded by this gene interacts with components
            of the origin recognition complex (ORC) and regulates the formation
            of the prereplicative complex. The encoded protein stabilizes the
            ORC and therefore aids in DNA replication. This protein is required
            for the G1/S phase transition of the cell cycle. In addition, the
            encoded protein binds to trimethylated histone H3 in
            heterochromatin and recruits the ORC and lysine methyltransferases,
            which help maintain the repressive heterochromatic state. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL133057.1, SRR1803614.4182.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292616.10/ ENSP00000292616.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2157              BC030547.1         42-2198
            2158-2160           BC009436.1         1949-1951
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..2160
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /note="leucine rich repeats and WD repeat domain
                     containing 1"
                     /db_xref="GeneID:222229"
                     /db_xref="HGNC:HGNC:21769"
                     /db_xref="MIM:615167"
     exon            1..177
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..2041
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich repeat and WD repeat-containing protein 1;
                     ORC-associated protein; origin recognition
                     complex-associated protein; centromere protein 33;
                     testicular tissue protein Li 4"
                     /codon_start=1
                     /product="leucine-rich repeat and WD repeat-containing
                     protein 1 isoform 1"
                     /protein_id="NP_690852.1"
                     /db_xref="CCDS:CCDS34715.1"
                     /db_xref="GeneID:222229"
                     /db_xref="HGNC:HGNC:21769"
                     /db_xref="MIM:615167"
                     /translation="MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLC
                     RLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNP
                     FLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEE
                     EAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPE
                     AGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPL
                     HFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHK
                     YKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHK
                     KAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCP
                     VASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNE
                     DIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKG
                     IVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVV
                     ANASFTYLTALTDSNIVAIWGRM"
     exon            178..412
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            413..529
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            530..670
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            671..775
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            776..901
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1016
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1117
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1325
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1398
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1539
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1631
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1787
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1900
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2160
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2137..2142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /note="hexamer: ATTAAA"
     polyA_site      2160
                     /gene="LRWD1"
                     /gene_synonym="CENP-33; ORCA"
                     /note="major polyA site"
ORIGIN      
        1 agttaccgcg gacgccagtg ccgggctcca ggagacgcag ggcgacgcca cacgccgggg
       61 tggccgactg ggtcagcgcg ggctgcgcct cctcgccatg ggccccctct cggcgcggct
      121 gctaatgcag cgcgggcgcc ccaagagcga ccggctgggg aagatccgga gtctggacct
      181 gtcaggattg gagctgcttt ccgagcacct ggaccccaaa ctcctgtgcc gcctgacgca
      241 gctgcaggag cttgacctgt ctaacaacca cctggagacg ctgccggaca acctgggcct
      301 gtcccacctg cgtgtcctcc gctgcgccaa caaccagctg ggggatgtta ctgccttgtg
      361 ccagttcccc aagctcgagg aactcagcct ggagggcaac cccttcctga cggtcaatga
      421 caacctgaaa gtctcctttc tcctgcccac gctccgtaag gtcaatggca aggatgcgtc
      481 ctcaacttac tctcaggtgg agaacctgaa tcgggagctg accagcaggg tcacagctca
      541 ctgggagaag ttcatggcca cactgggtcc tgaagaggag gctgagaagg cccaggcgga
      601 ctttgtgaag tcggctgtca gggatgtccg ctacgggccc gagtccctca gcgagttcac
      661 ccagtggcgg gtgcggatga tctctgagga gctggtggcc gccagtagga cccaggtgca
      721 aaaggctaac agcccagaga agcccccaga agctggagct gcccacaagc ccagggccag
      781 actggcggcc ttgaaacggc cagacgacgt cccactcagc ctctctccca gcaagcgggc
      841 gtgtgcctcc ccgtcggccc aggtggaggg cagccctgtg gcaggctccg atggcagcca
      901 gcctgctgtg aagctggagc ccctgcactt cctgcagtgc cacagcaaga acaacagccc
      961 ccaggacctc gagacccagc tgtgggcctg tgccttcgag ccggcctggg aggagggggc
     1021 cacatcccag accgtggcca cgtgcggcgg ggaggctgtg tgcgtaattg attgccagac
     1081 gggcatcgtg ctccacaagt acaaggcacc cggcgaggag ttcttttctg tggcctggac
     1141 cgctctgatg gtggtcacac aggctggcca caagaagcgc tggagtgtgc tggcggctgc
     1201 aggcctacgg ggcctggtcc ggctgctgca cgtgcgtgcc ggcttctgct gcggggtcat
     1261 ccgagcccac aagaaggcca tcgccaccct gtgcttcagc cccgcccacg agacccatct
     1321 cttcacggcc tcctatgaca agcggatcat cctctgggac atcggggtgc ccaaccagga
     1381 ctacgaattc caggccagcc agctgctcac actggacacc acctctatcc ccctgcgcct
     1441 ctgccctgtc gcctcctgcc cggacgcccg cctgctggcc ggctgcgagg gcggctgctg
     1501 ctgctgggac gtgcggctgg accagcccca aaagaggagg gtgtgtgaag tggaattcgt
     1561 cttctctgag ggctccgagg catctggacg gagagtggat gggctggcat ttgtgaatga
     1621 ggacatcgtg gcctccaagg ggagcggcct gggcaccatc tgcctgtgga gctggaggca
     1681 gacgtggggg ggccggggca gccagtccac ggtggcagtg gtggtcctgg cgcggctgca
     1741 atggtcgtcc accgagttgg cctacttctc gctcagcgcc tgccctgata aggggattgt
     1801 gctctgtggg gatgaggagg gcaacgtgtg gctctacgac gtcagcaaca tcctgaagca
     1861 gccacccctg ctgccggcag ccctgcaggc ccccacacag atcctgaagt ggccccagcc
     1921 ctgggccctt ggccaggtgg tgaccaagac catggtgaac acagtggtgg ccaatgcctc
     1981 cttcacctac ctcaccgccc tgacggactc caacatcgta gccatctggg ggaggatgta
     2041 gcctcacacc atcgcaaagg accagggaca cagctaacta acttattcag ctttgggccg
     2101 atgggggtgg gggggggtct ttcagtgaat atttttatta aactctactg tggacaagaa
//
