LOCUS       NM_006309               3490 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens LRR binding FLII interacting protein 2 (LRRFIP2),
            transcript variant 1, mRNA.
ACCESSION   NM_006309
VERSION     NM_006309.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3490)
  AUTHORS   Cho,I.T., Lim,Y., Golden,J.A. and Cho,G.
  TITLE     Aristaless Related Homeobox (ARX) Interacts with beta-Catenin,
            BCL9, and P300 to Regulate Canonical Wnt Signaling
  JOURNAL   PLoS One 12 (1), e0170282 (2017)
   PUBMED   28103279
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3490)
  AUTHORS   Burger,D., Fickentscher,C., de Moerloose,P. and Brandt,K.J.
  TITLE     F-actin dampens NLRP3 inflammasome activity via Flightless-I and
            LRRFIP2
  JOURNAL   Sci Rep 6, 29834 (2016)
   PUBMED   27431477
  REMARK    GeneRIF: Data show that the ability of Ca(2+) to accentuate the
            activity of NLRP3 inflammasome is abrogated in Flightless-I (FliI)
            and leucine-rich repeat FliI-interaction protein 2
            (LRRFIP2)-knockdown macrophages.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3490)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   6  (bases 1 to 3490)
  AUTHORS   Pinheiro,M., Pinto,C., Peixoto,A., Veiga,I., Mesquita,B.,
            Henrique,R., Baptista,M., Fragoso,M., Sousa,O., Pereira,H.,
            Marinho,C., Moreira Dias,L. and Teixeira,M.R.
  TITLE     A novel exonic rearrangement affecting MLH1 and the contiguous
            LRRFIP2 is a founder mutation in Portuguese Lynch syndrome families
  JOURNAL   Genet Med 13 (10), 895-902 (2011)
   PUBMED   21785361
  REMARK    GeneRIF: A novel exonic rearrangement affecting MLH1 and the
            contiguous LRRFIP2 is a founder mutation in Portuguese Lynch
            syndrome families
REFERENCE   7  (bases 1 to 3490)
  AUTHORS   Gunawardena,H.P., Huang,Y., Kenjale,R., Wang,H., Xie,L. and Chen,X.
  TITLE     Unambiguous characterization of site-specific phosphorylation of
            leucine-rich repeat Fli-I-interacting protein 2 (LRRFIP2) in
            Toll-like receptor 4 (TLR4)-mediated signaling
  JOURNAL   J Biol Chem 286 (13), 10897-10910 (2011)
   PUBMED   21220426
  REMARK    GeneRIF: Unambiguous characterization of site-specific
            phosphorylation of leucine-rich repeat Fli-I-interacting protein 2
            (LRRFIP2) in Toll-like receptor 4 (TLR4)-mediated signaling.
REFERENCE   8  (bases 1 to 3490)
  AUTHORS   Dai,P., Jeong,S.Y., Yu,Y., Leng,T., Wu,W., Xie,L. and Chen,X.
  TITLE     Modulation of TLR signaling by multiple MyD88-interacting partners
            including leucine-rich repeat Fli-I-interacting proteins
  JOURNAL   J Immunol 182 (6), 3450-3460 (2009)
   PUBMED   19265123
  REMARK    GeneRIF: LRR-binding MyD88 interactor LRRFIP2 is a positive
            regulator of NF-kappa B activity and is also a positive regulator
            of cytokine production in lipopolysaccharide-stimulated
            macrophages, suggesting a functional role in TLR4-mediated
            inflammatory response.
REFERENCE   9  (bases 1 to 3490)
  AUTHORS   Liu,J., Bang,A.G., Kintner,C., Orth,A.P., Chanda,S.K., Ding,S. and
            Schultz,P.G.
  TITLE     Identification of the Wnt signaling activator leucine-rich repeat
            in Flightless interaction protein 2 by a genome-wide functional
            analysis
  JOURNAL   Proc Natl Acad Sci U S A 102 (6), 1927-1932 (2005)
   PUBMED   15677333
  REMARK    GeneRIF: These data suggest that LRRFIP2 plays an important role in
            transducing Wnt signals.
REFERENCE   10 (bases 1 to 3490)
  AUTHORS   Fong,K.S. and de Couet,H.G.
  TITLE     Novel proteins interacting with the leucine-rich repeat domain of
            human flightless-I identified by the yeast two-hybrid system
  JOURNAL   Genomics 58 (2), 146-157 (1999)
   PUBMED   10366446
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC053668.1, AF115509.1,
            AK024692.1 and R27776.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006309.3.
            
            Summary: The protein encoded by this gene, along with MYD88, binds
            to the cytosolic tail of toll-like receptor 4 (TLR4), which results
            in activation of nuclear factor kappa B signaling. The
            ubiquitin-like protein FAT10 prevents the interaction of the
            encoded protein and TLR4, thereby inactivating the nuclear factor
            kappa B signaling pathway. In addition, this protein can
            downregulate the NLRP3 inflammasome by recruiting the caspase-1
            inhibitor Flightless-I to the inflammasome complex. [provided by
            RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a, also known as 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF115509.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336686.9/ ENSP00000338727.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BC053668.1         282-299
            19-2430             AF115509.1         1-2412
            2431-3453           AK024692.1         810-1832
            3454-3490           R27776.1           13-49               c
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..3490
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="LRR binding FLII interacting protein 2"
                     /db_xref="GeneID:9209"
                     /db_xref="HGNC:HGNC:6703"
                     /db_xref="MIM:614043"
     exon            1..44
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            45..189
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..2265
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="isoform a is encoded by transcript variant 1;
                     leucine-rich repeat flightless-interacting protein 2; LRR
                     FLII-interacting protein 2; leucine rich repeat (in FLII)
                     interacting protein 2"
                     /codon_start=1
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform a"
                     /protein_id="NP_006300.1"
                     /db_xref="CCDS:CCDS2664.1"
                     /db_xref="GeneID:9209"
                     /db_xref="HGNC:HGNC:6703"
                     /db_xref="MIM:614043"
                     /translation="MGTPASGRKRTPVKDRFSAEDEALSNIAREAEARLAAKRAARAE
                     ARDIRMRELERQQKEYSLHSFDRKWGQIQKWLEDSERARYSHRSSHHRPYLGVEDALS
                     IRSVGSHRYDMFKDRSSRLSSLNHSYSHSHGMKKRSSDSHKDLLSGLYFDQRNYSSLR
                     HSKPTSAYYTRQSSSLYSDPLATYKSDRASPTANSGLLRSASLASLYNGGLYNPYGPR
                     TPSECSYYSSRISSARSSPGFTNDDTASIVSSDRASRGRRESVVSAADYFSRSNRRGS
                     VVSEVDDISIPDLSSLDEKSDKQYAENYTRPSSRNSASATTPLSGNSSRRGSGDTSSL
                     IDPDTSLSELRDIYDLKDQIQDVEGRYMQGLKELKESLSEVEEKYKKAMVSNAQLDNE
                     KNNLIYQVDTLKDVIEEQEEQMAEFYRENEEKSKELERQKHMCSVLQHKMEELKEGLR
                     QRDELIEEKQRMQQKIDTMTKEVFDLQETLLWKDKKIGALEKQKEYIACLRNERDMLR
                     EELADLQETVKTGEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPL
                     DVRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLAGLQNGSDLQFIEMQR
                     DANRQISEYKFKLSKAEQDITTLEQSISRLEGQVLRYKTAAENAEKVEDELKAEKRKL
                     QRELRTALDKIEEMEMTNSHLAKRLEKMKANRTALLAQQ"
     exon            190..276
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..327
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            328..384
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            385..429
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            430..471
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            472..537
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            538..612
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            613..663
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            664..708
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            709..756
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            757..813
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            814..882
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            883..972
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1017
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1134
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1206
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1377
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1470
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1563
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1665
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1798
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1848
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1969
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2049
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2154
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..3490
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3089..3094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATACA"
     polyA_site      3112
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="major polyA site"
     regulatory      3428..3433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATAAA"
     polyA_site      3453
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
     regulatory      3464..3469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATAAA"
     polyA_site      3490
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
ORIGIN      
        1 tctagatgta aatgtcagtg gaaaggagag ctaccatcac taagggctga aaacacagtt
       61 aaatcacttt caaagttaaa agactattaa gaacacaaga tggggactcc tgcttctgga
      121 aggaaaagaa cacctgtgaa agaccgattt tctgcagaag atgaagcttt gagtaacatt
      181 gccagagagg cagaggcaag gctggcagca aaacgggctg cccgggcaga agcaagagat
      241 atacgcatga gagaactgga acgacaacaa aaagagtact ctcttcattc ctttgatcgg
      301 aagtggggac agattcagaa gtggctggaa gattcggaaa gggccaggta ttcccaccgg
      361 tccagtcacc atcgtcctta tctgggagtt gaggatgcat tgtccattcg aagtgttggc
      421 agtcacaggt atgatatgtt caaggataga tcatcaagac tttcatcatt aaatcattct
      481 tacagtcact ctcatggaat gaagaagagg tcttctgatt ctcataaaga cctactgagt
      541 ggcctctact ttgaccagag aaactatagc agtcttagac atagcaaacc cacctctgcc
      601 tactacactc ggcagtcttc ttccctgtac agtgaccctc tggcaacata taagagtgac
      661 agggcctctc ctactgcaaa ttctggtctg ctgagaagtg ccagtctggc atcattgtac
      721 aatggtggat tatataaccc ttatggtcct cgaactccat ctgaatgcag ttattattca
      781 tcaagaataa gttcagcccg aagcagtcca gggtttacca acgatgacac tgcaagcatt
      841 gtgtcttctg atcgtgccag tcgtggacga agggagagtg tggtatctgc cgctgattat
      901 ttcagtcgct ccaatcgtag gggaagtgtt gtctctgagg tggatgatat cagtatccca
      961 gatttgtcca gcttggatga aaaatctgac aaacagtatg ctgaaaatta tacaagacct
     1021 tcatctcgaa attctgcctc agcaacaacc cctctaagtg gaaactcatc cagacgagga
     1081 agtggggaca ccagcagctt aatagatcca gacacttcat taagtgaatt gcgggatatc
     1141 tatgacctta aggaccagat acaggatgta gaagggagat acatgcaggg gcttaaagaa
     1201 ctaaaggaat ctttgtctga agtggaagaa aaatacaaga aagccatggt ttccaatgca
     1261 cagttagaca atgagaagaa caatttgatc taccaagtag acacactcaa ggatgttatt
     1321 gaagagcagg aggaacagat ggcagaattt tatagagaaa atgaagaaaa atcaaaggag
     1381 ttagaaaggc agaaacatat gtgtagtgtg ctgcagcata agatggaaga acttaaagaa
     1441 ggcctgcggc aaagagatga gcttattgag gagaagcagc gcatgcagca gaaaatagac
     1501 accatgacaa aagaggtgtt tgacctccag gagacacttc tttggaaaga taaaaaaatt
     1561 ggggccctag agaaacagaa agaatacatt gcctgcctta ggaatgagcg agatatgctc
     1621 agagaggagc tggctgacct gcaggagaca gtgaagacgg gagagaaaca tggcttagtt
     1681 ataatccccg atggcactcc caatggtgat gtcagtcatg aaccagtggc tggagccatc
     1741 actgttgtgt ctcaggaagc tgctcaggtc ttggagtcag caggagaagg gccattagat
     1801 gtaaggctac gaaaacttgc tggagagaag gaagaactac tgtcacagat tagaaaactg
     1861 aagcttcagt tagaggagga acgacagaaa tgctccagga atgatggcac agtgggtgac
     1921 ctggcaggac tgcagaatgg ctcagacttg cagttcatcg aaatgcagag agatgccaat
     1981 agacaaatta gcgaatacaa atttaagctt tcaaaagcag aacaggatat aactaccttg
     2041 gagcaaagta ttagccggct tgagggacag gttctgagat ataaaactgc tgctgagaat
     2101 gctgagaaag ttgaagatga attgaaagca gaaaaacgga agctacaacg agagttacga
     2161 acagcactgg acaagattga ggagatggag atgaccaaca gccacctggc caagcggctg
     2221 gagaagatga aggccaatag gacagcactt ctggcccagc agtaggaaaa ccacccttca
     2281 acctgggtga tgctccttgg ggccctacct agagggactg acttttgtcc attgacacaa
     2341 acccctttta gtactgtttt gagttttgtc attaaaacag ccacctttgt attttataat
     2401 ttatgacaga atgaagtcat tttgaatcta catgaatgaa cactttggat tttgttgtag
     2461 tttgattcta gggtagaacc agtccatgct gtttttattt tttatctccg taattgtaga
     2521 atcatgttta ctcaacgttt ttccccagct gcctcagtaa ctgggcactc ggaggccttg
     2581 gcacgggttc tggaggacag acagcaattc tatgagtgct cactgagata cttgctggag
     2641 acctcagaaa acacaagtgc cttctccacg gtgcaattca gacttcagtg atctccagtg
     2701 gtcaaaagac atttaccctt aatatcagac aacatttata ttttagtgaa gaaacaagtt
     2761 ctcgggtggg gaatctatgt ttcactcaga tttatatgtt tggaggaaaa aagccttttt
     2821 ttgtaaaata tttaaattta tataagaaaa tgttagaaaa aaatatgggg agtgtatata
     2881 aaacttgctt tattgcatgg ggcaggggaa gtccaggcct aatactccta aagtaagagt
     2941 tgggtccttt ttttcttcaa tacaactgtg ctgtaccttg taaagtattt tatctgctgc
     3001 ttatttgtgg aatgaaacct caaacaaacc caaaggggga gggtagggca gggcaggcag
     3061 attggaaatc tgcctgcaga ttctattaaa tacacccttt tgccaaccac aattggctac
     3121 tgacatgttc attttgttat aggagacttt aaaacaaatc atggcaacca catccctctt
     3181 cttttgtctc cttctcaagg aaaaacaatc tggaatgtag aacttttaaa gacttgagtt
     3241 tcacctttag aggtaccctg tccaagaggt ttttttaaaa tcatggagtg caactacttt
     3301 gcagaattta tgattctctt ttgctcttag tacccaggtg gttttataga aatgattatt
     3361 gtatctttaa agtgcttaca cagaaaatat attgggggaa aatctagtga ctaaaggcac
     3421 ataagataat aaaactagac tttaaaaagg taactcaaaa gtgaataaaa tctctaatat
     3481 ttgttccttt
//
