LOCUS       NM_001348302            3360 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens LRR binding FLII interacting protein 2 (LRRFIP2),
            transcript variant 10, mRNA.
ACCESSION   NM_001348302 XM_005265554
VERSION     NM_001348302.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3360)
  AUTHORS   Cho,I.T., Lim,Y., Golden,J.A. and Cho,G.
  TITLE     Aristaless Related Homeobox (ARX) Interacts with beta-Catenin,
            BCL9, and P300 to Regulate Canonical Wnt Signaling
  JOURNAL   PLoS One 12 (1), e0170282 (2017)
   PUBMED   28103279
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3360)
  AUTHORS   Burger,D., Fickentscher,C., de Moerloose,P. and Brandt,K.J.
  TITLE     F-actin dampens NLRP3 inflammasome activity via Flightless-I and
            LRRFIP2
  JOURNAL   Sci Rep 6, 29834 (2016)
   PUBMED   27431477
  REMARK    GeneRIF: Data show that the ability of Ca(2+) to accentuate the
            activity of NLRP3 inflammasome is abrogated in Flightless-I (FliI)
            and leucine-rich repeat FliI-interaction protein 2
            (LRRFIP2)-knockdown macrophages.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3360)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   6  (bases 1 to 3360)
  AUTHORS   Pinheiro,M., Pinto,C., Peixoto,A., Veiga,I., Mesquita,B.,
            Henrique,R., Baptista,M., Fragoso,M., Sousa,O., Pereira,H.,
            Marinho,C., Moreira Dias,L. and Teixeira,M.R.
  TITLE     A novel exonic rearrangement affecting MLH1 and the contiguous
            LRRFIP2 is a founder mutation in Portuguese Lynch syndrome families
  JOURNAL   Genet Med 13 (10), 895-902 (2011)
   PUBMED   21785361
  REMARK    GeneRIF: A novel exonic rearrangement affecting MLH1 and the
            contiguous LRRFIP2 is a founder mutation in Portuguese Lynch
            syndrome families
REFERENCE   7  (bases 1 to 3360)
  AUTHORS   Gunawardena,H.P., Huang,Y., Kenjale,R., Wang,H., Xie,L. and Chen,X.
  TITLE     Unambiguous characterization of site-specific phosphorylation of
            leucine-rich repeat Fli-I-interacting protein 2 (LRRFIP2) in
            Toll-like receptor 4 (TLR4)-mediated signaling
  JOURNAL   J Biol Chem 286 (13), 10897-10910 (2011)
   PUBMED   21220426
  REMARK    GeneRIF: Unambiguous characterization of site-specific
            phosphorylation of leucine-rich repeat Fli-I-interacting protein 2
            (LRRFIP2) in Toll-like receptor 4 (TLR4)-mediated signaling.
REFERENCE   8  (bases 1 to 3360)
  AUTHORS   Dai,P., Jeong,S.Y., Yu,Y., Leng,T., Wu,W., Xie,L. and Chen,X.
  TITLE     Modulation of TLR signaling by multiple MyD88-interacting partners
            including leucine-rich repeat Fli-I-interacting proteins
  JOURNAL   J Immunol 182 (6), 3450-3460 (2009)
   PUBMED   19265123
  REMARK    GeneRIF: LRR-binding MyD88 interactor LRRFIP2 is a positive
            regulator of NF-kappa B activity and is also a positive regulator
            of cytokine production in lipopolysaccharide-stimulated
            macrophages, suggesting a functional role in TLR4-mediated
            inflammatory response.
REFERENCE   9  (bases 1 to 3360)
  AUTHORS   Liu,J., Bang,A.G., Kintner,C., Orth,A.P., Chanda,S.K., Ding,S. and
            Schultz,P.G.
  TITLE     Identification of the Wnt signaling activator leucine-rich repeat
            in Flightless interaction protein 2 by a genome-wide functional
            analysis
  JOURNAL   Proc Natl Acad Sci U S A 102 (6), 1927-1932 (2005)
   PUBMED   15677333
  REMARK    GeneRIF: These data suggest that LRRFIP2 plays an important role in
            transducing Wnt signals.
REFERENCE   10 (bases 1 to 3360)
  AUTHORS   Fong,K.S. and de Couet,H.G.
  TITLE     Novel proteins interacting with the leucine-rich repeat domain of
            human flightless-I identified by the yeast two-hybrid system
  JOURNAL   Genomics 58 (2), 146-157 (1999)
   PUBMED   10366446
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC126118.2 and AC006583.31.
            
            On Jan 26, 2017 this sequence version replaced XM_005265554.2.
            
            Summary: The protein encoded by this gene, along with MYD88, binds
            to the cytosolic tail of toll-like receptor 4 (TLR4), which results
            in activation of nuclear factor kappa B signaling. The
            ubiquitin-like protein FAT10 prevents the interaction of the
            encoded protein and TLR4, thereby inactivating the nuclear factor
            kappa B signaling pathway. In addition, this protein can
            downregulate the NLRP3 inflammasome by recruiting the caspase-1
            inhibitor Flightless-I to the inflammasome complex. [provided by
            RefSeq, Jan 2017].
            
            Transcript Variant: This variant (10) has a longer 5' UTR, and has
            multiple differences in the coding region but maintains the reading
            frame, compared to variant 1. The encoded isoform (f) is shorter,
            compared to isoform a. Variants 9 and 10 both encode the same
            isoform (f).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.31754.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-543               AC126118.2         57387-57929         c
            544-610             AC126118.2         56108-56174         c
            611-755             AC126118.2         30463-30607         c
            756-842             AC126118.2         10632-10718         c
            843-887             AC006583.31        23366-23410
            888-1004            AC006583.31        25118-25234
            1005-1076           AC006583.31        28488-28559
            1077-1247           AC006583.31        36220-36390
            1248-1340           AC006583.31        44910-45002
            1341-1433           AC006583.31        47144-47236
            1434-1535           AC006583.31        53701-53802
            1536-1668           AC006583.31        54084-54216
            1669-1718           AC006583.31        56235-56284
            1719-1839           AC006583.31        61116-61236
            1840-1919           AC006583.31        64861-64940
            1920-2024           AC006583.31        65511-65615
            2025-3360           AC006583.31        66065-67400
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..3360
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="LRR binding FLII interacting protein 2"
                     /db_xref="GeneID:9209"
                     /db_xref="HGNC:HGNC:6703"
                     /db_xref="MIM:614043"
     exon            1..543
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            544..610
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            611..755
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             666..2135
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="isoform f is encoded by transcript variant 10;
                     leucine-rich repeat flightless-interacting protein 2; LRR
                     FLII-interacting protein 2; leucine rich repeat (in FLII)
                     interacting protein 2"
                     /codon_start=1
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform f"
                     /protein_id="NP_001335231.1"
                     /db_xref="GeneID:9209"
                     /db_xref="HGNC:HGNC:6703"
                     /db_xref="MIM:614043"
                     /translation="MGTPASGRKRTPVKDRFSAEDEALSNIAREAEARLAAKRAARAE
                     ARDIRMRELERQQKELDEKSDKQYAENYTRPSSRNSASATTPLSGNSSRRGSGDTSSL
                     IDPDTSLSELRDIYDLKDQIQDVEGRYMQGLKELKESLSEVEEKYKKAMVSNAQLDNE
                     KNNLIYQVDTLKDVIEEQEEQMAEFYRENEEKSKELERQKHMCSVLQHKMEELKEGLR
                     QRDELIEEKQRMQQKIDTMTKEVFDLQETLLWKDKKIGALEKQKEYIACLRNERDMLR
                     EELADLQETVKTGEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPL
                     DVRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLAGLQNGSDLQFIEMQR
                     DANRQISEYKFKLSKAEQDITTLEQSISRLEGQVLRYKTAAENAEKVEDELKAEKRKL
                     QRELRTALDKIEEMEMTNSHLAKRLEKMKANRTALLAQQ"
     exon            756..842
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            843..887
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1004
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1076
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1247
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1340
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1433
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1535
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1668
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1718
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1839
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1919
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2024
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..3360
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2959..2964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATACA"
     polyA_site      2982
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="major polyA site"
     regulatory      3298..3303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATAAA"
     polyA_site      3323
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
     regulatory      3334..3339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
                     /note="hexamer: AATAAA"
     polyA_site      3360
                     /gene="LRRFIP2"
                     /gene_synonym="HUFI-2"
ORIGIN      
        1 gtcgtcgacc ctgtggcccc gcccatcctt cgcctcctca gtcggccgct caggctgcgg
       61 ctcctcccgg ggccggttcc cgctgtctca gccgcggcgc agagggcaca tcggcctgac
      121 cgactagccg gctgccagtg ccgggagccg ccgcgtccat ccccgcccgc ctggccgggg
      181 acccaggact gagtcgtcca gctgcgcccc tcgcccatag ccccagcggg cgccgggcgg
      241 gcgagcagct cttcggccct tcccggacag cgacgcagtc gcagcgcgcg ccctaacaca
      301 ggtaagggcc gctcctcccg ccagcccggt gggagcaggg agcggggagt tgggaacctt
      361 tttcccggcc tccgcagtcc cgtgggatcc gcgccgcctc cgacctcggc tagggggccg
      421 ggcttccctg gggactggcc ggcccgcggc gggctcccgg gctggggtcg ccccctgcct
      481 gcggcgttct cagacatccc gtctgatttc taacgctcac ccttccaccg cgctggggaa
      541 aagatgtggg aagataaatg aaggggtcta gatgtaaatg tcagtggaaa ggagagctac
      601 catcactaag ggctgaaaac acagttaaat cactttcaaa gttaaaagac tattaagaac
      661 acaagatggg gactcctgct tctggaagga aaagaacacc tgtgaaagac cgattttctg
      721 cagaagatga agctttgagt aacattgcca gagaggcaga ggcaaggctg gcagcaaaac
      781 gggctgcccg ggcagaagca agagatatac gcatgagaga actggaacga caacaaaaag
      841 agttggatga aaaatctgac aaacagtatg ctgaaaatta tacaagacct tcatctcgaa
      901 attctgcctc agcaacaacc cctctaagtg gaaactcatc cagacgagga agtggggaca
      961 ccagcagctt aatagatcca gacacttcat taagtgaatt gcgggatatc tatgacctta
     1021 aggaccagat acaggatgta gaagggagat acatgcaggg gcttaaagaa ctaaaggaat
     1081 ctttgtctga agtggaagaa aaatacaaga aagccatggt ttccaatgca cagttagaca
     1141 atgagaagaa caatttgatc taccaagtag acacactcaa ggatgttatt gaagagcagg
     1201 aggaacagat ggcagaattt tatagagaaa atgaagaaaa atcaaaggag ttagaaaggc
     1261 agaaacatat gtgtagtgtg ctgcagcata agatggaaga acttaaagaa ggcctgcggc
     1321 aaagagatga gcttattgag gagaagcagc gcatgcagca gaaaatagac accatgacaa
     1381 aagaggtgtt tgacctccag gagacacttc tttggaaaga taaaaaaatt ggggccctag
     1441 agaaacagaa agaatacatt gcctgcctta ggaatgagcg agatatgctc agagaggagc
     1501 tggctgacct gcaggagaca gtgaagacgg gagagaaaca tggcttagtt ataatccccg
     1561 atggcactcc caatggtgat gtcagtcatg aaccagtggc tggagccatc actgttgtgt
     1621 ctcaggaagc tgctcaggtc ttggagtcag caggagaagg gccattagat gtaaggctac
     1681 gaaaacttgc tggagagaag gaagaactac tgtcacagat tagaaaactg aagcttcagt
     1741 tagaggagga acgacagaaa tgctccagga atgatggcac agtgggtgac ctggcaggac
     1801 tgcagaatgg ctcagacttg cagttcatcg aaatgcagag agatgccaat agacaaatta
     1861 gcgaatacaa atttaagctt tcaaaagcag aacaggatat aactaccttg gagcaaagta
     1921 ttagccggct tgagggacag gttctgagat ataaaactgc tgctgagaat gctgagaaag
     1981 ttgaagatga attgaaagca gaaaaacgga agctacaacg agagttacga acagcactgg
     2041 acaagattga ggagatggag atgaccaaca gccacctggc caagcggctg gagaagatga
     2101 aggccaatag gacagcactt ctggcccagc agtaggaaaa ccacccttca acctgggtga
     2161 tgctccttgg ggccctacct agagggactg acttttgtcc attgacacaa acccctttta
     2221 gtactgtttt gagttttgtc attaaaacag ccacctttgt attttataat ttatgacaga
     2281 atgaagtcat tttgaatcta catgaatgaa cactttggat tttgttgtag tttgattcta
     2341 gggtagaacc agtccatgct gtttttattt tttatctccg taattgtaga atcatgttta
     2401 ctcaacgttt ttccccagct gcctcagtaa ctgggcactc ggaggccttg gcacgggttc
     2461 tggaggacag acagcaattc tatgagtgct cactgagata cttgctggag acctcagaaa
     2521 acacaagtgc cttctccacg gtgcaattca gacttcagtg atctccagtg gtcaaaagac
     2581 atttaccctt aatatcagac aacatttata ttttagtgaa gaaacaagtt ctcgggtggg
     2641 gaatctatgt ttcactcaga tttatatgtt tggaggaaaa aagccttttt ttgtaaaata
     2701 tttaaattta tataagaaaa tgttagaaaa aaatatgggg agtgtatata aaacttgctt
     2761 tattgcatgg ggcaggggaa gtccaggcct aatactccta aagtaagagt tgggtccttt
     2821 ttttcttcaa tacaactgtg ctgtaccttg taaagtattt tatctgctgc ttatttgtgg
     2881 aatgaaacct caaacaaacc caaaggggga gggtagggca gggcaggcag attggaaatc
     2941 tgcctgcaga ttctattaaa tacacccttt tgccaaccac aattggctac tgacatgttc
     3001 attttgttat aggagacttt aaaacaaatc atggcaacca catccctctt cttttgtctc
     3061 cttctcaagg aaaaacaatc tggaatgtag aacttttaaa gacttgagtt tcacctttag
     3121 aggtaccctg tccaagaggt ttttttaaaa tcatggagtg caactacttt gcagaattta
     3181 tgattctctt ttgctcttag tacccaggtg gttttataga aatgattatt gtatctttaa
     3241 agtgcttaca cagaaaatat attgggggaa aatctagtga ctaaaggcac ataagataat
     3301 aaaactagac tttaaaaagg taactcaaaa gtgaataaaa tctctaatat ttgttccttt
//
