LOCUS       NP_079337                796 aa            linear   PRI 28-APR-2025
DEFINITION  volume-regulated anion channel subunit LRRC8E isoform 1 [Homo
            sapiens].
ACCESSION   NP_079337
VERSION     NP_079337.2
DBSOURCE    REFSEQ: accession NM_025061.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 796)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (residues 1 to 796)
  AUTHORS   Lahey,L.J., Mardjuki,R.E., Wen,X., Hess,G.T., Ritchie,C.,
            Carozza,J.A., Bohnert,V., Maduke,M., Bassik,M.C. and Li,L.
  TITLE     LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of
            cGAMP
  JOURNAL   Mol Cell 80 (4), 578-591 (2020)
   PUBMED   33171122
REFERENCE   3  (residues 1 to 796)
  AUTHORS   Li,P., Hu,M., Wang,C., Feng,X., Zhao,Z., Yang,Y., Sahoo,N., Gu,M.,
            Yang,Y., Xiao,S., Sah,R., Cover,T.L., Chou,J., Geha,R.,
            Benavides,F., Hume,R.I. and Xu,H.
  TITLE     LRRC8 family proteins within lysosomes regulate cellular
            osmoregulation and enhance cell survival to multiple physiological
            stresses
  JOURNAL   Proc Natl Acad Sci U S A 117 (46), 29155-29165 (2020)
   PUBMED   33139539
REFERENCE   4  (residues 1 to 796)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (residues 1 to 796)
  AUTHORS   Lutter,D., Ullrich,F., Lueck,J.C., Kempa,S. and Jentsch,T.J.
  TITLE     Selective transport of neurotransmitters and modulators by distinct
            volume-regulated LRRC8 anion channels
  JOURNAL   J Cell Sci 130 (6), 1122-1133 (2017)
   PUBMED   28193731
REFERENCE   6  (residues 1 to 796)
  AUTHORS   Syeda,R., Qiu,Z., Dubin,A.E., Murthy,S.E., Florendo,M.N.,
            Mason,D.E., Mathur,J., Cahalan,S.M., Peters,E.C., Montal,M. and
            Patapoutian,A.
  TITLE     LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense
            Ionic Strength
  JOURNAL   Cell 164 (3), 499-511 (2016)
   PUBMED   26824658
REFERENCE   7  (residues 1 to 796)
  AUTHORS   Voss,F.K., Ullrich,F., Munch,J., Lazarow,K., Lutter,D., Mah,N.,
            Andrade-Navarro,M.A., von Kries,J.P., Stauber,T. and Jentsch,T.J.
  TITLE     Identification of LRRC8 heteromers as an essential component of the
            volume-regulated anion channel VRAC
  JOURNAL   Science 344 (6184), 634-638 (2014)
   PUBMED   24790029
REFERENCE   8  (residues 1 to 796)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   9  (residues 1 to 796)
  AUTHORS   Kubota,K., Kim,J.Y., Sawada,A., Tokimasa,S., Fujisaki,H.,
            Matsuda-Hashii,Y., Ozono,K. and Hara,J.
  TITLE     LRRC8 involved in B cell development belongs to a novel family of
            leucine-rich repeat proteins
  JOURNAL   FEBS Lett 564 (1-2), 147-152 (2004)
   PUBMED   15094057
  REMARK    GeneRIF: identified four genes, named TA-LRRP, AD158, LRRC5, and
            FLJ23420, as unknown LRRC8-like genes
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK091134.1, KF459574.1,
            BX538180.1 and CB049178.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 19, 2003 this sequence version replaced NP_079337.1.
            
            Summary: This gene encodes a member of a small, conserved family of
            proteins with similar structure, including a string of
            extracellular leucine-rich repeats. A related protein was shown to
            be involved in B-cell development. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Jun 2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Variants 1 and 2 encode the same protein (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538180.1, BC070089.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306708.11/ ENSP00000306524.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     Protein         1..796
                     /product="volume-regulated anion channel subunit LRRC8E
                     isoform 1"
                     /note="leucine-rich repeat-containing protein 8E;
                     volume-regulated anion channel subunit LRRC8E; leucine
                     rich repeat containing 8 family member E"
                     /calculated_mol_wt=90116
     Region          1..331
                     /region_name="Pannexin_like"
                     /note="Pannexin-like TM region of LRRC8; pfam12534"
                     /db_xref="CDD:463622"
     Site            23..43
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Site            63
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Site            117..137
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Site            266..286
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Site            302
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Site            314..334
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          486..508
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          508..529
                     /region_name="LRR 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          509..527
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          521..>772
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          536..557
                     /region_name="LRR 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          537..559
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          559..579
                     /region_name="LRR 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          560..583
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          583..604
                     /region_name="LRR 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          584..606
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          606..627
                     /region_name="LRR 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          607..631
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          631..652
                     /region_name="LRR 6"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          632..654
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          654..675
                     /region_name="LRR 7"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          655..677
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          677..698
                     /region_name="LRR 8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          678..700
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          700..721
                     /region_name="LRR 9"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          701..723
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          723..744
                     /region_name="LRR 10"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          724..746
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          746..767
                     /region_name="LRR 11"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6NSJ5.2)"
     Region          747..767
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..796
                     /gene="LRRC8E"
                     /coded_by="NM_025061.6:97..2487"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS12189.1"
                     /db_xref="GeneID:80131"
                     /db_xref="HGNC:HGNC:26272"
                     /db_xref="MIM:612891"
ORIGIN      
        1 mipvaefkqf teqqpafkvl kpwwdvlaey ltvamlmigv fgctlqvtqd kiiclpnhel
       61 qenlseapcq qllprgipeq igalqevkgl knnldlqqys finqlcyeta lhwyakyfpy
      121 lvvihtlifm vctsfwfkfp gtsskiehfi silgkcfdsp wttralsevs genqkgpaat
      181 eraaativam agtgpgkage gekekvlaep ekvvteppvv tlldkkegeq akalfekvkk
      241 frmhveegdi lytmyirqtv lkvckflail vynlvyveki sflvacrvet sevtgyasfc
      301 cnhtkahlfs klafcyisfv ciygltciyt lywlfhrplk eysfrsvree tgmgdipdvk
      361 ndfafmlhli dqydslyskr favflsevse srlkqlnlnh ewtpeklrqk lqrnaagrle
      421 lalcmlpglp dtvfelseve slrleaicdi tfppglsqlv hlqelsllhs parlpfslqv
      481 flrdhlkvmr vkceelrevp lwvfglrgle elhleglfpq elaraatles lrelkqlkvl
      541 slrsnagkvp asvtdvaghl qrlslhndga rlvalnslkk laalrelelv acgleripha
      601 vfslgalqel dlkdnhlrsi eeilsfqhcr klvtlrlwhn qiayvpehvr klrsleqlyl
      661 synkletlps qlglcsglrl ldvshnglhs lppevgllqn lqhlalsyna lealpeelff
      721 crklrtlllg dnqlsqlsph vgalralsrl elkgnrleal peelgncggl kkagllvedt
      781 lyqglpaevr dkmeee
//
