LOCUS       NM_032270               7170 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens leucine rich repeat containing 8 VRAC subunit C
            (LRRC8C), mRNA.
ACCESSION   NM_032270
VERSION     NM_032270.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Lahey,L.J., Mardjuki,R.E., Wen,X., Hess,G.T., Ritchie,C.,
            Carozza,J.A., Bohnert,V., Maduke,M., Bassik,M.C. and Li,L.
  TITLE     LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of
            cGAMP
  JOURNAL   Mol Cell 80 (4), 578-591 (2020)
   PUBMED   33171122
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Syeda,R., Qiu,Z., Dubin,A.E., Murthy,S.E., Florendo,M.N.,
            Mason,D.E., Mathur,J., Cahalan,S.M., Peters,E.C., Montal,M. and
            Patapoutian,A.
  TITLE     LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense
            Ionic Strength
  JOURNAL   Cell 164 (3), 499-511 (2016)
   PUBMED   26824658
REFERENCE   3  (bases 1 to 7170)
  AUTHORS   Xie,T., Deng,L., Mei,P., Zhou,Y., Wang,B., Zhang,J., Lin,J.,
            Wei,Y., Zhang,X. and Xu,R.
  TITLE     Genome-wide association study combining pathway analysis for
            typical sporadic amyotrophic lateral sclerosis in Chinese Han
            populations
  JOURNAL   Neurobiol Aging 35 (7), 1778 (2014)
   PUBMED   24529757
REFERENCE   4  (bases 1 to 7170)
  AUTHORS   Voss,F.K., Ullrich,F., Munch,J., Lazarow,K., Lutter,D., Mah,N.,
            Andrade-Navarro,M.A., von Kries,J.P., Stauber,T. and Jentsch,T.J.
  TITLE     Identification of LRRC8 heteromers as an essential component of the
            volume-regulated anion channel VRAC
  JOURNAL   Science 344 (6184), 634-638 (2014)
   PUBMED   24790029
REFERENCE   5  (bases 1 to 7170)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 7170)
  AUTHORS   Tominaga,K., Kondo,C., Kagata,T., Hishida,T., Nishizuka,M. and
            Imagawa,M.
  TITLE     The novel gene fad158, having a transmembrane domain and
            leucine-rich repeat, stimulates adipocyte differentiation
  JOURNAL   J Biol Chem 279 (33), 34840-34848 (2004)
   PUBMED   15184384
  REMARK    GeneRIF: fad158 has a role in regulating adipocyte differentiation
REFERENCE   7  (bases 1 to 7170)
  AUTHORS   Kubota,K., Kim,J.Y., Sawada,A., Tokimasa,S., Fujisaki,H.,
            Matsuda-Hashii,Y., Ozono,K. and Hara,J.
  TITLE     LRRC8 involved in B cell development belongs to a novel family of
            leucine-rich repeat proteins
  JOURNAL   FEBS Lett 564 (1-2), 147-152 (2004)
   PUBMED   15094057
  REMARK    GeneRIF: identified four genes, named TA-LRRP, AD158, LRRC5, and
            FLJ23420, as unknown LRRC8-like genes
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099569.2 and AC093423.2.
            
            On Nov 23, 2018 this sequence version replaced NM_032270.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.70857.1,
                                           SRR1803613.79000.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370454.9/ ENSP00000359483.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               AC099569.2         124182-124384
            204-345             AC093423.2         88319-88460
            346-7170            AC093423.2         114558-121382
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.2"
     gene            1..7170
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="leucine rich repeat containing 8 VRAC subunit C"
                     /db_xref="GeneID:84230"
                     /db_xref="HGNC:HGNC:25075"
                     /db_xref="MIM:612889"
     exon            1..203
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /inference="alignment:Splign:2.1.0"
     exon            204..345
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /inference="alignment:Splign:2.1.0"
     CDS             208..2619
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="factor for adipocyte differentiation 158; leucine
                     rich repeat containing 8C; leucine-rich repeat-containing
                     protein 8C; hypothetical protein AD158; leucine rich
                     repeat containing 8 family member C"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8C"
                     /protein_id="NP_115646.3"
                     /db_xref="CCDS:CCDS725.1"
                     /db_xref="GeneID:84230"
                     /db_xref="HGNC:HGNC:25075"
                     /db_xref="MIM:612889"
                     /translation="MIPVTEFRQFSEQQPAFRVLKPWWDVFTDYLSVAMLMIGVFGCT
                     LQVMQDKIICLPKRVQPAQNHSSLSNVSQAVASTTPLPPPKPSPANPITVEMKGLKTD
                     LDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFMLCSNFWFKFPGSSSKIEHFIS
                     ILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQSGPEDSLVNSQSLKSIPE
                     KFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLII
                     IAYNSALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTC
                     LYTLYWLFYRSLREYSFEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFL
                     SEVSENKLKQLNLNNEWTPDKLRQKLQTNAHNRLELPLIMLSGLPDTVFEITELQSLK
                     LEIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLKENLKVLSVKFDDMRELPP
                     WMYGLRNLEELYLVGSLSHDISRNVTLESLRDLKSLKILSIKSNVSKIPQAVVDVSSH
                     LQKMCIHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNL
                     KSIEEIVSFQHLRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPSHLFLC
                     NKIRYLDLSYNDIRFIPPEIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNS
                     LSVLSPKIGNLLFLSYLDVKGNHFEILPPELGDCRALKRAGLVVEDALFETLPSDVRE
                     QMKTE"
     exon            346..7170
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2761..2766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="hexamer: AATAAA"
     polyA_site      2793
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="major polyA site"
     regulatory      7145..7150
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="hexamer: AATAAA"
     polyA_site      7170
                     /gene="LRRC8C"
                     /gene_synonym="AD158; FAD158; TIMES"
                     /note="major polyA site"
ORIGIN      
        1 gttagctgcg tcccggctgg aagcccgcga agagaagtag gaggaaggcg ccgccgtggc
       61 cgcggccgca gtgctcgggc gcgacaagcc atgagcagcg accccgcggg cgacgccgcc
      121 gtccctggca gccgctgagc ccgaccccca ggcagctcgc cgctccctag caccttctcc
      181 agtcgacact ctcgcgcccg gagaaacatg attcccgtga cagaattccg gcagttctct
      241 gagcagcagc ctgccttccg agtgctgaag ccatggtggg atgtgtttac cgattacctc
      301 tcagtagcca tgctcatgat cggcgtgttt ggatgtactt tacaggtcat gcaagacaag
      361 ataatctgcc ttccgaaaag agtgcagcct gctcagaacc actcttccct ttcgaatgtc
      421 tctcaagcag ttgccagtac cactccactg cctccaccta aaccatctcc tgctaacccc
      481 atcactgtgg aaatgaaagg cctgaagaca gatttggacc ttcagcagta cagctttata
      541 aatcagatgt gttatgagcg agccctccac tggtatgcca agtatttccc ttaccttgtc
      601 ctcatccata ccctggtctt tatgctctgc agtaactttt ggttcaaatt ccctggttcc
      661 agctccaaaa tagaacattt catctccatt ctggggaagt gttttgactc tccttggacc
      721 acacgggctt tatctgaagt gtctggggag gactcagaag aaaaggacaa caggaagaac
      781 aacatgaaca ggtccaacac catccaatct ggtccagaag acagcctggt caactctcag
      841 tctttaaagt ccattcctga gaagtttgta gttgataaat ccactgcagg ggctctggat
      901 aaaaaggaag gtgagcaggc taaggcctta tttgagaagg tgaagaagtt caggctgcat
      961 gtggaagaag gtgatattct atatgccatg tatgttcgcc agactgtact taaagttatc
     1021 aaattcctaa tcatcattgc atataatagt gctctggttt ccaaggtcca gtttacagtg
     1081 gactgtaatg tggacattca ggacatgact ggatataaaa acttttcttg caatcatacc
     1141 atggcacact tgttctcaaa actgtccttt tgctatctgt gctttgttag tatctatgga
     1201 ttgacgtgcc tttatacctt atactggctg ttctaccgtt ctctacggga atattccttt
     1261 gagtatgtcc gtcaggagac tggaattgat gatattccag atgtgaaaaa tgactttgct
     1321 tttatgcttc atatgataga tcagtatgac cctctctatt ccaagagatt tgcagtgttc
     1381 ctgtctgaag tcagtgaaaa caaattaaag cagctgaact taaataacga atggactcct
     1441 gataaactga ggcagaagct acagacaaat gcccataatc gactggaatt gcctcttatc
     1501 atgctctctg gccttccaga cactgttttt gaaatcacag agttgcaatc tctaaaactt
     1561 gaaatcatta agaacgtaat gataccagcc accattgcac agctagacaa tcttcaagag
     1621 ctctctctgc accagtgttc tgtcaaaatc cacagtgcgg cgctctcttt cctgaaggaa
     1681 aacctcaagg tcttgagcgt caagtttgat gacatgaggg aactcccccc ctggatgtat
     1741 gggctccgaa atctggaaga gctgtaccta gttggctctc taagtcatga tatttccaga
     1801 aatgtcaccc ttgagtctct gcgggatctc aaaagcctta aaattctctc tatcaaaagc
     1861 aacgtttcca aaatccctca ggcagtggtt gatgtttcca gccatctcca gaagatgtgc
     1921 atacataatg atggcaccaa gctggtgatg ctcaacaact taaagaagat gaccaatctg
     1981 acagagctgg agctggtcca ctgtgacctg gagcgtattc ctcatgctgt gttcagccta
     2041 ctcagcctcc aggaattgga cctgaaggaa aacaatctga aatctataga agaaatcgtt
     2101 agctttcagc acttaagaaa gttgacagtg ctaaaactgt ggcataacag catcacctac
     2161 atcccagagc atataaagaa actcaccagc ctggaacgcc tgtcctttag tcacaataaa
     2221 atagaggtgc tgccttccca cctcttccta tgcaacaaga tccgatactt ggacttatcg
     2281 tacaatgaca ttcgatttat cccccctgaa attggagttc tacaaagttt acagtatttt
     2341 tccatcacat gtaacaaagt ggaaagcctt ccagatgaac tctacttctg caagaaactt
     2401 aaaactctga agattggaaa aaacagccta tctgtacttt caccgaaaat tggaaatttg
     2461 ctatttcttt cctacttaga tgtaaaaggt aatcactttg aaatcctccc tcctgaactg
     2521 ggtgactgtc gggctctgaa gcgagctggt ttagttgtag aagatgctct gtttgaaact
     2581 ctgccttctg acgtccggga gcaaatgaaa acagaataac ttatttttcg ttaaagtttg
     2641 actgaaacac gcttctacca aatacagtat aaataattag gtagtcttaa tgcctttcct
     2701 attttttttt ccttttcaca caaaatgtac acaaagatcg cgtaaggagt atgtattttt
     2761 aataaaaatt taattgtatt ttttcaatat taatattttg aagttttatt tgtcttgaaa
     2821 cacaatgtat ctattatcta ctgacagaaa gagatagttc catttggttt tttgtttttg
     2881 ttttttagtt cattcttgtg gaaaggaggg aattataagt tgcatgcttt ttggcatttt
     2941 ttaaatagag gaagatattt tgccaaggcc atttttaact tgtttacacc tgtacagggt
     3001 tcttattttt gttttcattg tgtgtgtatc aaggaatctg tgctagttat tttaatacca
     3061 gctgtcttct ccattggcca agaccttcat tcagtggaac ttcacataag tgcattgtca
     3121 tagagtattt taaatgttcc cctgcctcca acaaaaccct aaactgtgtt atttcatata
     3181 cctgacagtt attgttatcc ttaggcaagg tttttgtgag tgaagaaaat aatctttagt
     3241 ggctggtatg tttgaattag gcctttatca tttaacttat ttgcatttta ccaacatttt
     3301 aaaaatattt aaaacctaaa tacagaggct cctcccacct taatctcaat actgctatgt
     3361 agtactggtg aacaactttg gagaacctag atttatttta atattctaat agataattaa
     3421 attttaaaat gatagaagcc tggtttttga gtaatttaat catctttcat atcttgatat
     3481 gcagctgcat cagatggaaa aaaatttact aaagcaccct gttaaagagc tggtgtgctc
     3541 cagtgaggcc ctctcctttt ctcagcacag tgtggcagtg gtaatctatt caaattttgc
     3601 ctgttacaca aaataatcca cttatatata cagtcagcct tccgtacttg tgggttctgc
     3661 atctgtggat tccaccaact gtagatggaa aatactcaaa aagaaagttg tgtctgtact
     3721 gaatatgtac agactttttc cttgtcatta ttccctaaac aatacagcgt aacaactatt
     3781 taaataggat ttacattgtt ttaggtatta taaattacct agagatgatt taaagtatat
     3841 gggaggatgt gcataggtta tatgcaattt ctgcaccatt ttatgtcaag gattttgaac
     3901 atcctcagat tttggtgttc gagggcatcc tggaatcaat tccccatgat accgagggat
     3961 gactggatat taatcgattt ccctcaagcc tgcccttgct gtgaactagt gctacaagca
     4021 cgggaaatcc atctgctctc tggcttaaaa tatgtatctc atctaaaatt gttcgttaaa
     4081 gtgtgaataa attgctaagt ggttgttgct tagcaataat ttttgcctga ccatcagcag
     4141 agcttttatt tacctttgta attaaaatcc aacatagcat gggctcttgg gcaaaatatt
     4201 tcactgacta ttaagcatgt agaacaggaa aatgaaatac actatttgtg cccttgacct
     4261 agaaccagaa ccactaatta tgagaacttt gttttatgta ccaaattcac attgaccatt
     4321 ttctgagctc tagactgatc gtagtaagtt ttgtgactta acaatgaaag aaagtagaaa
     4381 gatgctttgg gttttcaaaa tgttgttttt taaaattgtt ctttgggtga atttactcag
     4441 tgctgctttg agttgtatac agaaacaaat gttgggaatt gctcctcaga aatgtgttcc
     4501 taagttgtgt tttcaacttt acatcatgag gtgaagcatt agggaaagag attctttcga
     4561 tttgtttaat aatctaatta tacagaccaa agtgctttac gtttctgctc tatgttaatg
     4621 ttttagaatg gtgattttgc tgattaattt agacctggaa ttgaaataat gtgctcagaa
     4681 taataacatg gttatagttc ttgtatgata aagtattcaa tttcagaata gtgttggaat
     4741 cctgagtttg aataatgtgt tgtatttaga aacatagccc tatctgtttt aaacaaataa
     4801 tttgttggcc gattgtccat ggttgagcat gacaaaaata cctcgtcgaa aggcaagctt
     4861 aggtaactgc tggcaaaaca ctgggtgcac tatttttctg gataaaattt atagttattt
     4921 tctatattac ccttcaaaag ggatctcttc aggttaaaaa tcacgcttat gctgaagtct
     4981 ttatctggtg ttaactaaaa atctcatatg ggttcataac cgaggtcact aataattcat
     5041 ttttatcact tgtaaaaatt tgctcaaaat tccaaaaaaa tattgatttg ttttttagtg
     5101 attttgcagg ctgaccccca acctaagttt tgataacatc tggtaagtca gtatagttct
     5161 gtgacttcat gttttaacta agaaggaaaa ttcatagata ttcctattag attttataaa
     5221 ccttcaaaag tctgaaactt aattttgagt ctaaattttc tgacactggc cccttttaat
     5281 attgtaagtt tttgttcact ttcttaagta aaaaaaacat ttaattacta gttagccctt
     5341 aactgggaaa ctcaggtaat gaactgctga cttttctaaa gttctttaac tgatcaattc
     5401 tgtatagagg gatatttatc taaccacttt ccgtatttta caagtgctct ttctaaaaag
     5461 gaataactat tatagctcta tttccccaat ctctatagga ctcatgagag attgcttgtg
     5521 taaatataaa agcaccatat gtgttcttaa ctcctatggc tgcttgaagc tcatgatgaa
     5581 aaagtctttt tgtcagtttt aattgttaag tacagaacaa acaattgttt ggtgatggcc
     5641 tggttgaaag agagcatata aatatatccc agtggaactc accaaagaag accacacctc
     5701 agaaattatt gcattttctc attatgtgtt gggtttgatt tgcttttgtt tttaatgcag
     5761 ctcttttaat ataaagattc ttgatacagt gaaatctctt atttcaagtg taagttattc
     5821 ttcacccacc ccttcccctg ccattgtatt tcccatctgt ttcaaggagt ttcaacaatt
     5881 tacattgcat cgtatgcagt aggtactgct ttttcagaaa gacctggaaa acatacctgc
     5941 tatgaatatt ttgttcagat gtagccattt acctggctct caagttgtct ttgtggagag
     6001 ggtcttagac aaaaatcttc cttgtattta cttgggttaa gtgaagtcca aattcttaca
     6061 gtatgctatt tcaggatttc tgatattaaa aaagaaaaaa caaaatcttt atatctctta
     6121 ttaacacttc ccccaagaag ggttgtgctg ttatttattt tctattataa gaaaagttca
     6181 ttctttaagt agtttctttt acctctaatc taatttcata ccaaatacct gatcaataga
     6241 aatgatatat ttaagcagca aagattccta atccatcatt atgaaaagtg tcagcatact
     6301 tagtagtgaa cagataaagt caatttgaat ataattccac tttgttttta gagactaaat
     6361 taagattcaa ttaacattat cctatgaatt ctgaatgtga taatgtgatt caaacagtca
     6421 aattttatta agctcttagt aactcaggat agcattccat actaacctca agttagcaaa
     6481 acaaattagt taaacagctt ggttcttagc agactgctta aaagatcaag aaaattttct
     6541 catcttttct ttctacttag aaacattgca agaaaccttg gacagtcttc accagacctg
     6601 ccatgatttt ataagattta ggcctcagtg acatgctcct gaaagtttcc tgccagccat
     6661 ccaaactaag catccactca ttccatcttc ccaaagtcac tcaccgataa aggtagcatc
     6721 cttaagttca tttttgaaag gtggaggagg atctccccct gcccaaagga atttttttat
     6781 cagaatacct tgaaaggggg gtatataaat ttggaaaact taatttcttg gctgtgtttg
     6841 ataacagttc ctatgcatgg tttttaatgt gaggtaaatt tgtttctttc ttcagaatac
     6901 ctctctccac cccccacctt attcttcctc tttaatgaat atttttattg gagctcaaac
     6961 tccatgactt acgtgctcac taagttttct tttttcccct tgtttactct gtctgtatgt
     7021 atgtcaaaag ctggcaaaac ctctaaaact gtcaagaaaa tgcttgaaag ttgatttgtc
     7081 atagtgcaaa ttcatgataa aatgtcttaa tgttatttgg atatgtagta catagaaaca
     7141 gaaaaataaa gtcattttta taacttaaaa
//
