LOCUS       NM_022143               4195 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens leucine rich repeat containing 4 (LRRC4), mRNA.
ACCESSION   NM_022143
VERSION     NM_022143.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Deng,K. and Wu,M.
  TITLE     Leucine-rich repeats containing 4 protein (LRRC4) in memory,
            psychoneurosis, and glioblastoma
  JOURNAL   Chin Med J (Engl) 136 (1), 4-12 (2023)
   PUBMED   36780420
  REMARK    GeneRIF: Leucine-rich repeats containing 4 protein (LRRC4) in
            memory, psychoneurosis, and glioblastoma.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Feng,J., Zhang,Y., Ren,X., Li,D., Fu,H., Liu,C., Zhou,W., Liu,Q.,
            Liu,Q. and Wu,M.
  TITLE     Leucine-rich repeat containing 4 act as an autophagy inhibitor that
            restores sensitivity of glioblastoma to temozolomide
  JOURNAL   Oncogene 39 (23), 4551-4566 (2020)
   PUBMED   32372061
  REMARK    GeneRIF: Leucine-rich repeat containing 4 act as an autophagy
            inhibitor that restores sensitivity of glioblastoma to
            temozolomide.
REFERENCE   3  (bases 1 to 4195)
  AUTHORS   Aoyama,T., Takasawa,A., Takasawa,K., Ono,Y., Emori,M., Murata,M.,
            Hayasaka,T., Fujitani,N., Osanai,M., Yamashita,T., Hasegawa,T. and
            Sawada,N.
  TITLE     Identification of Coiled-Coil Domain-Containing Protein 180 and
            Leucine-Rich Repeat-Containing Protein 4 as Potential
            Immunohistochemical Markers for Liposarcoma Based on Proteomic
            Analysis Using Formalin-Fixed, Paraffin-Embedded Tissue
  JOURNAL   Am J Pathol 189 (5), 1015-1028 (2019)
   PUBMED   30790560
  REMARK    GeneRIF: High LRRC4 expression is associated with Liposarcoma .
REFERENCE   4  (bases 1 to 4195)
  AUTHORS   Wang,Z., Guo,Q., Wang,R., Xu,G., Li,P., Sun,Y., She,X., Liu,Q.,
            Chen,Q., Yu,Z., Liu,C., Xiong,J., Li,G. and Wu,M.
  TITLE     The D Domain of LRRC4 anchors ERK1/2 in the cytoplasm and
            competitively inhibits MEK/ERK activation in glioma cells
  JOURNAL   J Hematol Oncol 9 (1), 130 (2016)
   PUBMED   27884160
  REMARK    GeneRIF: Following EGF stimuli, the D domain of LRRC4 anchors
            ERK1/2 in the cytoplasm and abrogates ERK1/2 activation and nuclear
            translocation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4195)
  AUTHORS   Li,Y., Zhao,C., Yu,Z., Chen,J., She,X., Li,P., Liu,C., Zhang,Y.,
            Feng,J., Fu,H., Wang,B., Kuang,L., Li,L., Lv,G. and Wu,M.
  TITLE     Low expression of miR-381 is a favorite prognosis factor and
            enhances the chemosensitivity of osteosarcoma
  JOURNAL   Oncotarget 7 (42), 68585-68596 (2016)
   PUBMED   27612424
  REMARK    GeneRIF: Results show a strong negative correlation between the
            expression of miR-381 and LRRC4 in osteosarcoma (OS) tissues,
            indicating LRRC4 as a direct target gene of miR-381.
REFERENCE   6  (bases 1 to 4195)
  AUTHORS   Zhang,Q.H., Wang,L.L., Cao,L., Peng,C., Li,X.L., Tang,K., Li,W.F.,
            Liao,P., Wang,J.R. and Li,G.Y.
  TITLE     Study of a novel brain relatively specific gene LRRC4 involved in
            glioma tumorigenesis suppression using the Tet-on system
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 37 (8), 532-540 (2005)
   PUBMED   16077900
REFERENCE   7  (bases 1 to 4195)
  AUTHORS   Zhang,Q., Wang,J., Fan,S., Wang,L., Cao,L., Tang,K., Peng,C.,
            Li,Z., Li,W., Gan,K., Liu,Z., Li,X., Shen,S. and Li,G.
  TITLE     Expression and functional characterization of LRRC4, a novel
            brain-specific member of the LRR superfamily
  JOURNAL   FEBS Lett 579 (17), 3674-3682 (2005)
   PUBMED   15967442
  REMARK    GeneRIF: LRRC4 may play an important role in maintaining normal
            function and suppressing tumorigenesis in the central nervous
            system.
REFERENCE   8  (bases 1 to 4195)
  AUTHORS   Lin,J.C., Ho,W.H., Gurney,A. and Rosenthal,A.
  TITLE     The netrin-G1 ligand NGL-1 promotes the outgrowth of
            thalamocortical axons
  JOURNAL   Nat Neurosci 6 (12), 1270-1276 (2003)
   PUBMED   14595443
REFERENCE   9  (bases 1 to 4195)
  AUTHORS   Wang,J.R., Li,X.L., Fan,S.Q., Tan,C., Xiang,J.J., Tang,K., Wang,R.
            and Li,G.Y.
  TITLE     Expression of LRRC4 has the potential to decrease the growth rate
            and tumorigenesis of glioblastoma cell line U251
  JOURNAL   Ai Zheng 22 (9), 897-902 (2003)
   PUBMED   12969517
REFERENCE   10 (bases 1 to 4195)
  AUTHORS   Nakashiba,T., Ikeda,T., Nishimura,S., Tashiro,K., Honjo,T.,
            Culotti,J.G. and Itohara,S.
  TITLE     Netrin-G1: a novel glycosyl phosphatidylinositol-linked mammalian
            netrin that is functionally divergent from classical netrins
  JOURNAL   J Neurosci 20 (17), 6540-6550 (2000)
   PUBMED   10964959
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC008039.1, AF196976.4, BC111745.1 and BU682120.1.
            
            On Nov 22, 2018 this sequence version replaced NM_022143.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF196976.4, BC111561.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249363.4/ ENSP00000249363.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-488               AC008039.1         69229-69716         c
            489-2822            AF196976.4         4-2337
            2823-3802           BC111745.1         2329-3308
            3803-4195           BU682120.1         17-409              c
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.1"
     gene            1..4195
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="leucine rich repeat containing 4"
                     /db_xref="GeneID:64101"
                     /db_xref="HGNC:HGNC:15586"
                     /db_xref="MIM:610486"
     exon            1..525
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..4195
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             626..2587
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="brain tumor associated protein LRRC4; leucine-rich
                     repeat-containing protein 4; netrin-G2 ligand; brain
                     tumor-associated protein BAG; nasopharyngeal
                     carcinoma-associated gene 14 protein; netrin-G ligand-2"
                     /codon_start=1
                     /product="leucine-rich repeat-containing protein 4
                     precursor"
                     /protein_id="NP_071426.1"
                     /db_xref="CCDS:CCDS5799.1"
                     /db_xref="GeneID:64101"
                     /db_xref="HGNC:HGNC:15586"
                     /db_xref="MIM:610486"
                     /translation="MKLLWQVTVHHHTWNAILLPFVYLTAQVWILCAAIAAAASAGPQ
                     NCPSVCSCSNQFSKVVCTRRGLSEVPQGIPSNTRYLNLMENNIQMIQADTFRHLHHLE
                     VLQLGRNSIRQIEVGAFNGLASLNTLELFDNWLTVIPSGAFEYLSKLRELWLRNNPIE
                     SIPSYAFNRVPSLMRLDLGELKKLEYISEGAFEGLFNLKYLNLGMCNIKDMPNLTPLV
                     GLEELEMSGNHFPEIRPGSFHGLSSLKKLWVMNSQVSLIERNAFDGLASLVELNLAHN
                     NLSSLPHDLFTPLRYLVELHLHHNPWNCDCDILWLAWWLREYIPTNSTCCGRCHAPMH
                     MRGRYLVEVDQASFQCSAPFIMDAPRDLNISEGRMAELKCRTPPMSSVKWLLPNGTVL
                     SHASRHPRISVLNDGTLNFSHVLLSDTGVYTCMVTNVAGNSNASAYLNVSTAELNTSN
                     YSFFTTVTVETTEISPEDTTRKYKPVPTTSTGYQPAYTTSTTVLIQTTRVPKQVAVPA
                     TDTTDKMQTSLDEVMKTTKIIIGCFVAVTLLAAAMLIVFYKLRKRHQQRSTVTAARTV
                     EIIQVDEDIPAATSAAATAAPSGVSGEGAVVLPTIHDHINYNTYKPAHGAHWTENSLG
                     NSLHPTVTTISEPYIIQTHTKDKVQETQI"
     regulatory      2725..2730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2758
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="major polyA site"
     regulatory      4173..4178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="hexamer: AATAAA"
     polyA_site      4195
                     /gene="LRRC4"
                     /gene_synonym="NAG14; NGL-2"
                     /note="major polyA site"
ORIGIN      
        1 gaccgcaatc agctgccgcc ggcccggagc ggccgacgcc gggagaggga gcgcagcatc
       61 cagcgagtgc ggcctgggcg gcttcggggc gcccccgcac tccctccggc ggcggcccgc
      121 agagcgggcc ggggccgccg gagcgcactt tggcgatgaa gcgccttccc ccgcggctgg
      181 agcgagccgg tgccagggtc caaggacaaa gccgggaagc gctggtggcg ccgctccttg
      241 ggcggagggg ggggcatgca cctgcatagc ccgccgggcc ttggctctcc caggcgcgcc
      301 tcaactccgc ggcttcgccg gctccggctc cggatttact agaggccatt ttgtctcccc
      361 caccccttcc ctcccctccc ctcccctccc ctctgccctc cccgccccca ccacttcccc
      421 cttttgttaa ttaaaactaa gaagtcggaa tgggaacgag gtgcccagct cccgtggaga
      481 aagcttaagg acaccacgcc agtgctttcc tgccttcctt ccgagatgga aagaggagct
      541 cctagctcac ttaagccggg gtagggctgg ttctcctttc cgagccaaaa tcccaggcga
      601 tggtgaatta tgaacgtgcc acaccatgaa gctcttgtgg caggtaactg tgcaccacca
      661 cacctggaat gccatcctgc tcccgttcgt ctacctcacg gcgcaagtgt ggattctgtg
      721 tgcagccatc gctgctgccg cctcagccgg gccccagaac tgcccctccg tctgctcgtg
      781 cagtaaccag ttcagcaagg tggtgtgcac gcgccggggc ctctccgagg tcccgcaggg
      841 tattccctcg aacacccggt acctcaacct catggagaac aacatccaga tgatccaggc
      901 cgacaccttc cgccacctcc accacctgga ggtcctgcag ttgggcagga actccatccg
      961 gcagattgag gtgggggcct tcaacggcct ggccagcctc aacaccctgg agctgttcga
     1021 caactggctg acagtcatcc ctagcggggc ctttgaatac ctgtccaagc tgcgggagct
     1081 ctggcttcgc aacaacccca tcgaaagcat cccctcttac gccttcaacc gggtgccctc
     1141 cctcatgcgc ctggacttgg gggagctcaa gaagctggag tatatctctg agggagcttt
     1201 tgaggggctg ttcaacctca agtatctgaa cttgggcatg tgcaacatta aagacatgcc
     1261 caatctcacc cccctggtgg ggctggagga gctggagatg tcagggaacc acttccctga
     1321 gatcaggcct ggctccttcc atggcctgag ctccctcaag aagctctggg tcatgaactc
     1381 acaggtcagc ctgattgagc ggaatgcttt tgacgggctg gcttcacttg tggaactcaa
     1441 cttggcccac aataacctct cttctttgcc ccatgacctc tttaccccgc tgaggtacct
     1501 ggtggagttg catctacacc acaacccttg gaactgtgat tgtgacattc tgtggctagc
     1561 ctggtggctt cgagagtata tacccaccaa ttccacctgc tgtggccgct gtcatgctcc
     1621 catgcacatg cgaggccgct acctcgtgga ggtggaccag gcctccttcc agtgctctgc
     1681 ccccttcatc atggacgcac ctcgagacct caacatttct gagggtcgga tggcagaact
     1741 taagtgtcgg actcccccta tgtcctccgt gaagtggttg ctgcccaatg ggacagtgct
     1801 cagccacgcc tcccgccacc caaggatctc tgtcctcaac gacggcacct tgaacttttc
     1861 ccacgtgctg ctttcagaca ctggggtgta cacatgcatg gtgaccaatg ttgcaggcaa
     1921 ctccaacgcc tcggcctacc tcaatgtgag cacggctgag cttaacacct ccaactacag
     1981 cttcttcacc acagtaacag tggagaccac ggagatctcg cctgaggaca caacgcgaaa
     2041 gtacaagcct gttcctacca cgtccactgg ttaccagccg gcatatacca cctctaccac
     2101 ggtgctcatt cagactaccc gtgtgcccaa gcaggtggca gtacccgcga cagacaccac
     2161 tgacaagatg cagaccagcc tggatgaagt catgaagacc accaagatca tcattggctg
     2221 ctttgtggca gtgactctgc tagctgccgc catgttgatt gtcttctata aacttcgtaa
     2281 gcggcaccag cagcggagta cagtcacagc cgcccggact gttgagataa tccaggtgga
     2341 cgaagacatc ccagcagcaa catccgcagc agcaacagca gctccgtccg gtgtatcagg
     2401 tgagggggca gtagtgctgc ccacaattca tgaccatatt aactacaaca cctacaaacc
     2461 agcacatggg gcccactgga cagaaaacag cctggggaac tctctgcacc ccacagtcac
     2521 cactatctct gaaccttata taattcagac ccataccaag gacaaggtac aggaaactca
     2581 aatatgactc ccctccccca aaaaacttat aaaatgcaat agaatgcaca caaagacagc
     2641 aacttttgta cagagtgggg agagactttt tcttgtatat gcttatatat taagtctatg
     2701 ggctggttaa aaaaaacaga ttatattaaa atttaaagac aaaaagtcaa aacaaaaata
     2761 ttttctaact tgtaagttct atttaaaggg ggtggggggg aatcttggga acgttgtggg
     2821 gtacaagcca caagttaact tgctatgctg ccagaaggga tttctggtat aaggttgaaa
     2881 ttgctgagat aaaataaact aaaacaacaa acatccttaa agaggtaggg tgtgggctgc
     2941 tgagggggca agagggatag actgaatctg tcatttttta gaagatgctt cataggacac
     3001 aggactatcc atttctacag acatctttct taagccgaga gctgtctttg cagaattatc
     3061 ttattaaaaa aaaatacaaa aaaaaagcac catgaatttg tactgtgcca aaatgatagt
     3121 ggcaataata tttttcttca gaggctaagg ggaagataga cctgtacatg tattttaagc
     3181 aggaaaacca ccaaaattca aacgaaccct taaaggggct gagtctttgt ccatgtgcac
     3241 aagtcttggg ggtctgtgca ctggacacac atgtgccttg cctgacagaa ttgcttagaa
     3301 aggagcagag aaggacggga gctggcgtag ctctcagtct aatgctttca cattgcacca
     3361 gattcctgca gttgggctta acccactctg caaggcatgt gtctattcag ggtttcccag
     3421 gaggaggagg cccctgaagg ggtgcggcag caggggggtg ggaagggagg gtaaagcaga
     3481 gagaatgtat tcacaggagg ccataaatta gcattgtgcc atgtccacct gttgttcctg
     3541 gactctgaca catgattgta gacaatgatg gcattttatg atttttaaaa aagattttgt
     3601 ttgttttaag gtggagaggg aggagaagcc tgaattgggg agcttgggag tggaagcttt
     3661 tctcatgctt gtgatttggt gactcttcta aatgctgatt cattagggat tattacagcg
     3721 gatgtcaact ggttgtggtg atggggtggg ggcagaacat gagtagcaaa gaaaagggag
     3781 agacgaaaag ggtacaggct cacgtaaagt aatgaagcct ttcccaacca ggatggttca
     3841 caaaaaagct gtgagatcca cagttacaag tgacacgcat gcacacatgg agttccacat
     3901 ggggtactgt tgaatggagc acactgatgg gaagcttccc tctcccaggc cccctcagga
     3961 gaaggtggaa gagaaagggt gggcgtgggt ggggaaggca gagatctctg ttattctggt
     4021 ccatctaatg atgtaacttc tttcagttat tattttagct ttaaaaggaa ataaggggtt
     4081 cagatttgaa agggggtggt atgggtgggg gaggggaatt gccaacaaaa gaacactgat
     4141 tgtaatgtga gaaaagtgta tttttgtatt ttaataaata ttgttgaatt tttaa
//
