LOCUS       NM_033300               7194 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens LDL receptor related protein 8 (LRP8), transcript
            variant 2, mRNA.
ACCESSION   NM_033300
VERSION     NM_033300.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Hu,G., Che,P., Deng,L., Liu,L., Liao,J. and Liu,Q.
  TITLE     MiR-378a-5p exerts a radiosensitizing effect on CRC through
            LRP8/beta-catenin axis
  JOURNAL   Cancer Biol Ther 25 (1), 2308165 (2024)
   PUBMED   38389136
  REMARK    GeneRIF: MiR-378a-5p exerts a radiosensitizing effect on CRC
            through LRP8/beta-catenin axis.
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Fuentealba,L.M., Pizarro,H. and Marzolo,M.P.
  TITLE     OCRL1 Deficiency Affects the Intracellular Traffic of ApoER2 and
            Impairs Reelin-Induced Responses
  JOURNAL   Biomolecules 14 (7), 799 (2024)
   PUBMED   39062513
  REMARK    GeneRIF: OCRL1 Deficiency Affects the Intracellular Traffic of
            ApoER2 and Impairs Reelin-Induced Responses.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7194)
  AUTHORS   Whately,K.M., Sengottuvel,N., Edatt,L., Srivastava,S., Woods,A.T.,
            Tsai,Y.S., Porrello,A., Zimmerman,M.P., Chack,A.C., Jefferys,S.R.,
            Yacovone,G., Kim,D.J., Dudley,A.C., Amelio,A.L. and Pecot,C.V.
  TITLE     Spon1+ inflammatory monocytes promote collagen remodeling and lung
            cancer metastasis through lipoprotein receptor 8 signaling
  JOURNAL   JCI Insight 9 (9), e168792 (2024)
   PUBMED   38716730
  REMARK    GeneRIF: Spon1+ inflammatory monocytes promote collagen remodeling
            and lung cancer metastasis through lipoprotein receptor 8
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7194)
  AUTHORS   Caracci,M.O., Pizarro,H., Alarcon-Godoy,C., Fuentealba,L.M.,
            Farfan,P., De Pace,R., Santibanez,N., Cavieres,V.A., Pastor,T.P.,
            Bonifacino,J.S., Mardones,G.A. and Marzolo,M.P.
  TITLE     The Reelin receptor ApoER2 is a cargo for the adaptor protein
            complex AP-4: Implications for Hereditary Spastic Paraplegia
  JOURNAL   Prog Neurobiol 234, 102575 (2024)
   PUBMED   38281682
  REMARK    GeneRIF: The Reelin receptor ApoER2 is a cargo for the adaptor
            protein complex AP-4: Implications for Hereditary Spastic
            Paraplegia.
            Erratum:[Prog Neurobiol. 2024 Oct;241:102662. doi:
            10.1016/j.pneurobio.2024.102662.. PMID: 39294072]
REFERENCE   5  (bases 1 to 7194)
  AUTHORS   Suzuki,K., Iwata,Y., Matsuzaki,H., Anitha,A., Suda,S., Iwata,K.,
            Shinmura,C., Kameno,Y., Tsuchiya,K.J., Nakamura,K., Takei,N. and
            Mori,N.
  TITLE     Reduced expression of apolipoprotein E receptor type 2 in
            peripheral blood lymphocytes from patients with major depressive
            disorder
  JOURNAL   Prog Neuropsychopharmacol Biol Psychiatry 34 (6), 1007-1010 (2010)
   PUBMED   20493228
  REMARK    GeneRIF: the expression of ApoER2 may serve as a trait marker for
            major depressive disorder.
REFERENCE   6  (bases 1 to 7194)
  AUTHORS   Riddell,D.R., Vinogradov,D.V., Stannard,A.K., Chadwick,N. and
            Owen,J.S.
  TITLE     Identification and characterization of LRP8 (apoER2) in human blood
            platelets
  JOURNAL   J Lipid Res 40 (10), 1925-1930 (1999)
   PUBMED   10508213
REFERENCE   7  (bases 1 to 7194)
  AUTHORS   Sun,X.M. and Soutar,A.K.
  TITLE     Expression in vitro of alternatively spliced variants of the
            messenger RNA for human apolipoprotein E receptor-2 identified in
            human tissues by ribonuclease protection assays
  JOURNAL   Eur J Biochem 262 (1), 230-239 (1999)
   PUBMED   10231386
REFERENCE   8  (bases 1 to 7194)
  AUTHORS   Clatworthy,A.E., Stockinger,W., Christie,R.H., Schneider,W.J.,
            Nimpf,J., Hyman,B.T. and Rebeck,G.W.
  TITLE     Expression and alternate splicing of apolipoprotein E receptor 2 in
            brain
  JOURNAL   Neuroscience 90 (3), 903-911 (1999)
   PUBMED   10218790
REFERENCE   9  (bases 1 to 7194)
  AUTHORS   Kim,D.H., Magoori,K., Inoue,T.R., Mao,C.C., Kim,H.J., Suzuki,H.,
            Fujita,T., Endo,Y., Saeki,S. and Yamamoto,T.T.
  TITLE     Exon/intron organization, chromosome localization, alternative
            splicing, and transcription units of the human apolipoprotein E
            receptor 2 gene
  JOURNAL   J Biol Chem 272 (13), 8498-8504 (1997)
   PUBMED   9079678
REFERENCE   10 (bases 1 to 7194)
  AUTHORS   Kim,D.H., Iijima,H., Goto,K., Sakai,J., Ishii,H., Kim,H.J.,
            Suzuki,H., Kondo,H., Saeki,S. and Yamamoto,T.
  TITLE     Human apolipoprotein E receptor 2. A novel lipoprotein receptor of
            the low density lipoprotein receptor family predominantly expressed
            in brain
  JOURNAL   J Biol Chem 271 (14), 8373-8380 (1996)
   PUBMED   8626535
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL355483.14, DB455425.1,
            AL119002.1, CD300289.1, BC006443.1 and AL606760.9.
            
            On May 31, 2019 this sequence version replaced NM_033300.3.
            
            Summary: This gene encodes a member of the low density lipoprotein
            receptor (LDLR) family. Low density lipoprotein receptors are cell
            surface proteins that play roles in both signal transduction and
            receptor-mediated endocytosis of specific ligands for lysosomal
            degradation. The encoded protein plays a critical role in the
            migration of neurons during development by mediating Reelin
            signaling, and also functions as a receptor for the cholesterol
            transport protein apolipoprotein E. Expression of this gene may be
            a marker for major depressive disorder. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (2) lacks two consecutive coding
            exons but maintains the reading frame, compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                AL355483.14        63932-64020         c
            90-551              DB455425.1         1-462
            552-654             AL355483.14        16537-16639         c
            655-759             AL355483.14        7192-7296           c
            760-904             AL119002.1         1-145
            905-905             AL355483.14        2593-2593           c
            906-1497            AL119002.1         147-738
            1498-1805           CD300289.1         200-507
            1806-2923           BC006443.1         1-1118
            2924-7194           AL606760.9         97204-101474        c
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..7194
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="LDL receptor related protein 8"
                     /db_xref="GeneID:7804"
                     /db_xref="HGNC:HGNC:6700"
                     /db_xref="MIM:602600"
     exon            1..282
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..2540
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; ApoE receptor 2; low density lipoprotein
                     receptor-related protein 8, apolipoprotein e receptor"
                     /codon_start=1
                     /product="low-density lipoprotein receptor-related protein
                     8 isoform 2 precursor"
                     /protein_id="NP_150643.2"
                     /db_xref="CCDS:CCDS580.1"
                     /db_xref="GeneID:7804"
                     /db_xref="HGNC:HGNC:6700"
                     /db_xref="MIM:602600"
                     /translation="MGLPEPGPLRLLALLLLLLLLLLLQLQHLAAAAADPLLGGQGPA
                     KDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHER
                     WKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADE
                     AGCATWLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQ
                     ICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLI
                     PMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAV
                     DWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQ
                     AKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKT
                     LISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIF
                     HELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYR
                     APQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAP
                     SISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWK
                     RKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGET
                     REPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLP"
     exon            283..402
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..525
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..654
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            655..774
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            775..900
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1075
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1303
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1422
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1562
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1704
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1857
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..2082
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2151
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2324
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2501
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..7194
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3991..3996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: AATAAA"
     polyA_site      4013
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="major polyA site"
     regulatory      5982..5987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: AATAGA"
     polyA_site      6003
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
     regulatory      6658..6663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: TATAAA"
     polyA_site      6680
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
ORIGIN      
        1 gaaatgcagc cgaggagccg gggcgggcgg cagcggcggc ggcggcggcg gcgggggcag
       61 cggcaacccc ggcgccgcgg caaggactcg gagggctgag acgcggcggc ggcggcgcgg
      121 ggagcgcggg gcgcggcggc cggagccccg ggcccgccat gggcctcccc gagccgggcc
      181 ctctccggct tctggcgctg ctgctgctgc tgctgctgct gctgctgctg cagctccagc
      241 atcttgcggc ggcagcggct gatccgctgc tcggcggcca agggccggcc aaggattgcg
      301 aaaaggacca attccagtgc cggaacgagc gctgcatccc ctctgtgtgg agatgcgacg
      361 aggacgatga ctgcttagac cacagcgacg aggacgactg ccccaagaag acctgtgcag
      421 acagtgactt cacctgtgac aacggccact gcatccacga acggtggaag tgtgacggcg
      481 aggaggagtg tcctgatggc tccgatgagt ccgaggccac ttgcaccaag caggtgtgtc
      541 ctgcagagaa gctgagctgt ggacccacca gccacaagtg tgtacctgcc tcgtggcgct
      601 gcgacgggga gaaggactgc gagggtggag cggatgaggc cggctgtgct acctggctga
      661 acgagtgtct gcacaacaat ggcggctgct cacacatctg cactgacctc aagattggct
      721 ttgaatgcac gtgcccagca ggcttccagc tcctggacca gaagacctgt ggcgacattg
      781 atgagtgcaa ggacccagat gcctgcagcc agatctgtgt caattacaag ggctatttta
      841 agtgtgagtg ctaccctggc tacgagatgg acctactgac caagaactgc aaggctgctg
      901 ctggcaagag cccatcccta atcttcacca accggcacga ggtgcggagg atcgacctgg
      961 tgaagcggaa ctattcacgc ctcatcccca tgctcaagaa tgtcgtggca ctagatgtgg
     1021 aagttgccac caatcgcatc tactggtgtg acctctccta ccgtaagatc tatagcgcct
     1081 acatggacaa ggccagtgac ccgaaagagc aggaggtcct cattgacgag cagttgcact
     1141 ctccagaggg cctggcagtg gactgggtcc acaagcacat ctactggact gactcgggca
     1201 ataagaccat ctcagtggcc acagttgatg gtggccgccg acgcactctc ttcagccgta
     1261 acctcagtga accccgggcc atcgctgttg accccctgcg agggttcatg tattggtctg
     1321 actgggggga ccaggccaag attgagaaat ctgggctcaa cggtgtggac cggcaaacac
     1381 tggtgtcaga caatattgaa tggcccaacg gaatcaccct ggatctgctg agccagcgct
     1441 tgtactgggt agactccaag ctacaccaac tgtccagcat tgacttcagt ggaggcaaca
     1501 gaaagacgct gatctcctcc actgacttcc tgagccaccc ttttgggata gctgtgtttg
     1561 aggacaaggt gttctggaca gacctggaga acgaggccat tttcagtgca aatcggctca
     1621 atggcctgga aatctccatc ctggctgaga acctcaacaa cccacatgac attgtcatct
     1681 tccatgagct gaagcagcca agagctccag atgcctgtga gctgagtgtc cagcctaatg
     1741 gaggctgtga atacctgtgc cttcctgctc ctcagatctc cagccactct cccaagtaca
     1801 catgtgcctg tcctgacaca atgtggctgg gtccagacat gaagaggtgc taccgagcac
     1861 ctcaatctac ctcaactacg acgttagctt ctaccatgac gaggacagta cctgccacca
     1921 caagagcccc cgggaccacc gtccacagat ccacctacca gaaccacagc acagagacac
     1981 caagcctgac agctgcagtc ccaagctcag ttagtgtccc cagggctccc agcatcagcc
     2041 cgtctaccct aagccctgca accagcaacc actcccagca ctatgcaaat gaagacagta
     2101 agatgggctc aacagtcact gccgctgtta tcgggatcat cgtgcccata gtggtgatag
     2161 ccctcctgtg catgagtgga tacctgatct ggagaaactg gaagcggaag aacaccaaaa
     2221 gcatgaattt tgacaaccca gtctacagga aaacaacaga agaagaagac gaagatgagc
     2281 tccatatagg gagaactgct cagattggcc atgtctatcc tgcagcaatc agcagctttg
     2341 atcgcccact gtgggcagag ccctgtcttg gggagaccag agaaccggaa gacccagccc
     2401 ctgccctcaa ggagcttttt gtcttgccgg gggaaccaag gtcacagctg caccaactcc
     2461 cgaagaaccc tctttccgag ctgcctgtcg tcaaatccaa gcgagtggca ttaagccttg
     2521 aagatgatgg actaccctga ggatgggatc acccccttcg tgcctcatgg aattcagtcc
     2581 catgcactac actctggatg gtgtatgact ggatgaatgg gtttctatat atgggtctgt
     2641 gtgagtgtat gtgtgtgtgt gatttttttt ttaaatttat gttgcggaaa ggtaaccaca
     2701 aagttatgat gaactgcaaa catccaaagg atgtgagagt ttttctatgt ataatgtttt
     2761 atacactttt taactggttg cactacccat gaggaattcg tggaatggct actgctgact
     2821 aacatgatgc acataaccaa atgggggcca atggcacagt accttactca tcatttaaaa
     2881 actatattta cagaagatgt ttggttgctg ggggggcttt tttaggtttt ggggcatttg
     2941 ttttttgtaa ataagatgat tatgctttgt ggctatccat caacataagt aaaaaaaaaa
     3001 aaaaaacact tcaactccct cccccattta gattatttat taacatattt taaaaatcag
     3061 atgagttcta taaataattt agagaagtga gagtatttat ttttggcatg tttggcccac
     3121 cacacagact ctgtgtgtgt atgtgtgtgt ttatatgtgt atgtgtgtga cagaaaaatc
     3181 tgtagagaag aggcacatct atggctactg ttcaaataca taaagataaa tttattttca
     3241 cacagtccac aaggggtata tcttgtagtt ttcagaaaag cctttggaaa tctggatcag
     3301 aaaatagata ccatggtttg tgcaattatg tagtaaaaaa ggcaaatctt ttcacctctg
     3361 gctattcctg agaccccagg aagtcaggaa aagcctttca gctcacccat ggctgctgtg
     3421 actcctacca gggctttctt ggctttggcg aaggtcagtg tacagacatt ccatggtacc
     3481 agagtgctca gaaactcaag ataggatatg cctcaccctc agctactcct tgttttaaag
     3541 ttcagctctt tgagtaactt cttcaatttc tttcaggaca cttgggttga attcagtaag
     3601 tttcctctga agcaccctga agggtgccat ccttacagag ctaagtggag acgtttccag
     3661 atcagcccaa gtttactata gagactggcc caggcactga atgtctagga catgctgtgg
     3721 atgaagataa agatggtgga ataggtttta tcacatctct tatttctctt ttccccttac
     3781 tctctaccat ttcctttatg tggggaaaca ttttaaggta ataaataggt tacttaccat
     3841 catatgttca tatagatgaa actaattttt ggcttaagtc agaacaactg gccaaaattg
     3901 aagtcatatt tgagggggga aatggcatac gcaatattat attatattgg atatttatgt
     3961 tcacacagga atttggttta ctgctttgta aataaaagga aaaactccgg gtatatgtat
     4021 agatgttctt cattatagac atcttctttg cttttcttgg ccttggggga ggaagggaga
     4081 agtgctcttt tctacttgtg gggtctccca ttggaaacat aatcctatag tcccagaagg
     4141 attcagtccc cagtggcttt cccatccaaa gagaaagagt ttgagtttct taactctgct
     4201 gttctgccac ttactcccac tagacaacca gggacaaggt gcaacatgga agtgtttgac
     4261 ttaagtagga gcagaggagc tgcatctaat ctcatcatac ctggaacttg acacacttaa
     4321 gcaaatgcct tcccatccct acctgccaga tgcccccaac tcaatgaagt tggatgtctc
     4381 accagcttga taccctttga attttcagtc agacattctg gagttctagc atcctgtacc
     4441 taggaccttc ctctgtgtca ctcttggcct cctaaactct aagaaaataa ctatattctg
     4501 gagcttgggc agtgtgtttt gcataatcca gcaatctcct catgacatgc atgtgttgat
     4561 agtcctgaaa cattcattga gagggtaaat gcagttgacc tagaatgacc aataccaaac
     4621 agaattttaa gaacaggtgg ccaactccta tggagcttac tcacatatta ctattctttt
     4681 aagaacggaa agtaaaatta tttttgactg aagaaaaatg atgacagtga aaaacatgga
     4741 aatgtactca aaacaagtga ctttttctgt aaccttccaa agaaactgaa ttttccaagg
     4801 aattaaatga taacagtggc taaggcatag tttctaaact ttcagtaaga tcctggcatt
     4861 cacagaaaaa aatgatgaat ggggtctgga catacagcct gagatctcaa aatgacaatg
     4921 aaattcacaa ctttttctca gagacattca tgtttcctgc atatgctaca actgcagttt
     4981 gaaagaggca gcaatgggag caacccttta caagaaacaa attgtgatat attcatgtgt
     5041 tggacggcag taaataagat gaaacctgag gagtcagatc caccttcccc cattcataga
     5101 ggcttttcag cctcattttg aggtacagtt acatatcttt tgccttttgc ccccgtgcat
     5161 agctatctac agccaatcac agatcacaga gtcactggac tatagagctg gaaggaagct
     5221 cagagacaat gccaaggggg cagaaaattt atcagaagcc agtcccagtg cgtttcctcc
     5281 atttccttct gcaggaagac tattttgggc tgcctgaaca ttgtatcaaa cctgctacct
     5341 atactatggt ctacctttcc tccagtggaa ttacaaaggc actaactgaa atgccttcta
     5401 gaaacagaga aaacgaaact gtacttattt actcttgata cacagattat ttataaaaca
     5461 gattgaagta acctgttaac tggcaaaaag agaatgagat cggatttaaa tgtatggcag
     5521 taagtcctat tgatccctcc agttatctca gtatgactgc agtatattca ttcactaaaa
     5581 ccactcacta gataccaact acacacctgg cactgcagat gtaaaggtca gtcacacatg
     5641 ttctgacttt acagagttca cagtagcagt ggaggatgat atatgtggaa acaaaaaagg
     5701 cattgattct attcagagca ctgttagggc tcaaaggaga gaggggtctt tccacctaag
     5761 aaatgaggaa tagggtcatc atagaagtga ccttaagtct taaaaattaa gaaggggatt
     5821 ccaagctgct tcagacagag acacatcgag ctaaaacaca gaggtatgaa agagcacagg
     5881 gactttagga attgcacagt tcattctaac aggaacaaaa ggctcaaggg gggcaagaaa
     5941 tgaggctgta tggaaagaga ttcaatgtaa gcactttata aaatagatta atttctgatt
     6001 caatgaagca tttcttgatc attgtgtaca aggcactaca tgcatcatgg aaaattcatt
     6061 aggatgcatt gccagcactt tgcagaactg atattattca gcctcaagct ttccagtggc
     6121 caaagggaaa tgctgactgc ttttcatata tttgagtcaa agatttttta tatggtcaat
     6181 gaagactaat ataagggcag tgggattttc acagatgcat gccatgttgt cgagagcctc
     6241 ttagattttc tcaactgtga gaaagaaaaa cgaaaatgtt gaagacgttg agtctggaga
     6301 ggggatacta atcactgtcc agttgggcac tggtgggaat ggggaaatgg cacaggaatg
     6361 caagcctctc caccctaccc cccgaactcc agccatacac tcatcgtttc acaaaatata
     6421 aatgagttag cattaaatgt ttcagagtaa ataattcctt ttcccgaaat gcatgaagat
     6481 agagtaacag acttctcaca ctgtattttt agggtatgga gaatttagaa ggttaaagaa
     6541 ttactgcttc aatttttcag ttaaaaaaaa atcaggaagc tctgttcatt caggctatgc
     6601 accatgtgca cagtcaagaa ttagcagaaa ccctctgcat ttacaaacac tttgtgctat
     6661 aaaaaagtaa tttttaaaaa gccacgtgtg tgtgtgtgta tatatatata tatatatata
     6721 tttaaagcca aggttttgat acttttttac aaaaactaca agagaaaaca aatatacctg
     6781 tccaaaccat atacttttaa aagagcattt ttttttccat acaagctgtt gttaatttgg
     6841 gggtaaagtg ctgatttgca aacttcatca aattgttccc aagtggattc tccttgtttg
     6901 tctcccccta ccaaccccaa agttaccata tttgatgtaa gaatcaggca tgttagaatg
     6961 ttgtgtcaca ctaactgatt ctgctctttt tgtcttgtca ttcaagttcc gttagcttct
     7021 gtacgcggtg ccctttgcag tctggtgtct cttccagagg cgagggggct gaggatgggg
     7081 tgctgcatct cactagctat actggcatca tcttggtaaa ctgaaaacca aatgtggaca
     7141 tttgtaaaat cagtgcactg tttctagaga gagattaaat tcatttaaaa aaaa
//
