LOCUS       NM_004631               7704 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens LDL receptor related protein 8 (LRP8), transcript
            variant 1, mRNA.
ACCESSION   NM_004631
VERSION     NM_004631.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Hu,G., Che,P., Deng,L., Liu,L., Liao,J. and Liu,Q.
  TITLE     MiR-378a-5p exerts a radiosensitizing effect on CRC through
            LRP8/beta-catenin axis
  JOURNAL   Cancer Biol Ther 25 (1), 2308165 (2024)
   PUBMED   38389136
  REMARK    GeneRIF: MiR-378a-5p exerts a radiosensitizing effect on CRC
            through LRP8/beta-catenin axis.
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Fuentealba,L.M., Pizarro,H. and Marzolo,M.P.
  TITLE     OCRL1 Deficiency Affects the Intracellular Traffic of ApoER2 and
            Impairs Reelin-Induced Responses
  JOURNAL   Biomolecules 14 (7), 799 (2024)
   PUBMED   39062513
  REMARK    GeneRIF: OCRL1 Deficiency Affects the Intracellular Traffic of
            ApoER2 and Impairs Reelin-Induced Responses.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7704)
  AUTHORS   Whately,K.M., Sengottuvel,N., Edatt,L., Srivastava,S., Woods,A.T.,
            Tsai,Y.S., Porrello,A., Zimmerman,M.P., Chack,A.C., Jefferys,S.R.,
            Yacovone,G., Kim,D.J., Dudley,A.C., Amelio,A.L. and Pecot,C.V.
  TITLE     Spon1+ inflammatory monocytes promote collagen remodeling and lung
            cancer metastasis through lipoprotein receptor 8 signaling
  JOURNAL   JCI Insight 9 (9), e168792 (2024)
   PUBMED   38716730
  REMARK    GeneRIF: Spon1+ inflammatory monocytes promote collagen remodeling
            and lung cancer metastasis through lipoprotein receptor 8
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7704)
  AUTHORS   Caracci,M.O., Pizarro,H., Alarcon-Godoy,C., Fuentealba,L.M.,
            Farfan,P., De Pace,R., Santibanez,N., Cavieres,V.A., Pastor,T.P.,
            Bonifacino,J.S., Mardones,G.A. and Marzolo,M.P.
  TITLE     The Reelin receptor ApoER2 is a cargo for the adaptor protein
            complex AP-4: Implications for Hereditary Spastic Paraplegia
  JOURNAL   Prog Neurobiol 234, 102575 (2024)
   PUBMED   38281682
  REMARK    GeneRIF: The Reelin receptor ApoER2 is a cargo for the adaptor
            protein complex AP-4: Implications for Hereditary Spastic
            Paraplegia.
            Erratum:[Prog Neurobiol. 2024 Oct;241:102662. doi:
            10.1016/j.pneurobio.2024.102662.. PMID: 39294072]
REFERENCE   5  (bases 1 to 7704)
  AUTHORS   Suzuki,K., Iwata,Y., Matsuzaki,H., Anitha,A., Suda,S., Iwata,K.,
            Shinmura,C., Kameno,Y., Tsuchiya,K.J., Nakamura,K., Takei,N. and
            Mori,N.
  TITLE     Reduced expression of apolipoprotein E receptor type 2 in
            peripheral blood lymphocytes from patients with major depressive
            disorder
  JOURNAL   Prog Neuropsychopharmacol Biol Psychiatry 34 (6), 1007-1010 (2010)
   PUBMED   20493228
  REMARK    GeneRIF: the expression of ApoER2 may serve as a trait marker for
            major depressive disorder.
REFERENCE   6  (bases 1 to 7704)
  AUTHORS   Riddell,D.R., Vinogradov,D.V., Stannard,A.K., Chadwick,N. and
            Owen,J.S.
  TITLE     Identification and characterization of LRP8 (apoER2) in human blood
            platelets
  JOURNAL   J Lipid Res 40 (10), 1925-1930 (1999)
   PUBMED   10508213
REFERENCE   7  (bases 1 to 7704)
  AUTHORS   Sun,X.M. and Soutar,A.K.
  TITLE     Expression in vitro of alternatively spliced variants of the
            messenger RNA for human apolipoprotein E receptor-2 identified in
            human tissues by ribonuclease protection assays
  JOURNAL   Eur J Biochem 262 (1), 230-239 (1999)
   PUBMED   10231386
REFERENCE   8  (bases 1 to 7704)
  AUTHORS   Clatworthy,A.E., Stockinger,W., Christie,R.H., Schneider,W.J.,
            Nimpf,J., Hyman,B.T. and Rebeck,G.W.
  TITLE     Expression and alternate splicing of apolipoprotein E receptor 2 in
            brain
  JOURNAL   Neuroscience 90 (3), 903-911 (1999)
   PUBMED   10218790
REFERENCE   9  (bases 1 to 7704)
  AUTHORS   Kim,D.H., Magoori,K., Inoue,T.R., Mao,C.C., Kim,H.J., Suzuki,H.,
            Fujita,T., Endo,Y., Saeki,S. and Yamamoto,T.T.
  TITLE     Exon/intron organization, chromosome localization, alternative
            splicing, and transcription units of the human apolipoprotein E
            receptor 2 gene
  JOURNAL   J Biol Chem 272 (13), 8498-8504 (1997)
   PUBMED   9079678
REFERENCE   10 (bases 1 to 7704)
  AUTHORS   Kim,D.H., Iijima,H., Goto,K., Sakai,J., Ishii,H., Kim,H.J.,
            Suzuki,H., Kondo,H., Saeki,S. and Yamamoto,T.
  TITLE     Human apolipoprotein E receptor 2. A novel lipoprotein receptor of
            the low density lipoprotein receptor family predominantly expressed
            in brain
  JOURNAL   J Biol Chem 271 (14), 8373-8380 (1996)
   PUBMED   8626535
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL355483.14, DR002432.1,
            D50678.1, BC006443.1 and AL606760.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004631.4.
            
            Summary: This gene encodes a member of the low density lipoprotein
            receptor (LDLR) family. Low density lipoprotein receptors are cell
            surface proteins that play roles in both signal transduction and
            receptor-mediated endocytosis of specific ligands for lysosomal
            degradation. The encoded protein plays a critical role in the
            migration of neurons during development by mediating Reelin
            signaling, and also functions as a receptor for the cholesterol
            transport protein apolipoprotein E. Expression of this gene may be
            a marker for major depressive disorder. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D50678.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306052.12/ ENSP00000303634.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               AL355483.14        63911-64020         c
            111-788             DR002432.1         7-684
            789-898             D50678.1           733-842
            899-943             AL355483.14        12740-12784         c
            944-1410            D50678.1           888-1354
            1411-1446           AL355483.14        2562-2597           c
            1447-2779           D50678.1           1391-2723
            2780-3433           BC006443.1         465-1118
            3434-7704           AL606760.9         97204-101474        c
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..7704
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="LDL receptor related protein 8"
                     /db_xref="GeneID:7804"
                     /db_xref="HGNC:HGNC:6700"
                     /db_xref="MIM:602600"
     exon            1..282
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..3050
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ApoE receptor 2; low density lipoprotein
                     receptor-related protein 8, apolipoprotein e receptor"
                     /codon_start=1
                     /product="low-density lipoprotein receptor-related protein
                     8 isoform 1 precursor"
                     /protein_id="NP_004622.2"
                     /db_xref="CCDS:CCDS578.1"
                     /db_xref="GeneID:7804"
                     /db_xref="HGNC:HGNC:6700"
                     /db_xref="MIM:602600"
                     /translation="MGLPEPGPLRLLALLLLLLLLLLLQLQHLAAAAADPLLGGQGPA
                     KDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHER
                     WKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADE
                     AGCATLCAPHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGG
                     GACIPERWVCDRQFDCEDRSDEAAELCGRPGPGATSAPAACATASQFACRSGECVHLG
                     WRCDGDRDCKDKSDEADCPLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCL
                     QGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVN
                     YKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLK
                     NVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVH
                     KHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIE
                     KSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISS
                     TDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELK
                     QPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQS
                     TSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISP
                     STLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNT
                     KSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPE
                     DPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLP"
     exon            283..402
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            403..525
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..654
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            655..1041
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1164
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1284
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1410
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1585
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1813
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1932
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2072
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2214
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2367
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2592
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2661
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2834
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..3011
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..7704
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4501..4506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: AATAAA"
     polyA_site      4523
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="major polyA site"
     regulatory      6492..6497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: AATAGA"
     polyA_site      6513
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
     regulatory      7168..7173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
                     /note="hexamer: TATAAA"
     polyA_site      7190
                     /gene="LRP8"
                     /gene_synonym="APOER2; HSZ75190; LRP-8; MCI1"
ORIGIN      
        1 gaaatgcagc cgaggagccg gggcgggcgg cagcggcggc ggcggcggcg gcgggggcag
       61 cggcaacccc ggcgccgcgg caaggactcg gagggctgag acgcggcggc ggcggcgcgg
      121 ggagcgcggg gcgcggcggc cggagccccg ggcccgccat gggcctcccc gagccgggcc
      181 ctctccggct tctggcgctg ctgctgctgc tgctgctgct gctgctgctg cagctccagc
      241 atcttgcggc ggcagcggct gatccgctgc tcggcggcca agggccggcc aaggattgcg
      301 aaaaggacca attccagtgc cggaacgagc gctgcatccc ctctgtgtgg agatgcgacg
      361 aggacgatga ctgcttagac cacagcgacg aggacgactg ccccaagaag acctgtgcag
      421 acagtgactt cacctgtgac aacggccact gcatccacga acggtggaag tgtgacggcg
      481 aggaggagtg tcctgatggc tccgatgagt ccgaggccac ttgcaccaag caggtgtgtc
      541 ctgcagagaa gctgagctgt ggacccacca gccacaagtg tgtacctgcc tcgtggcgct
      601 gcgacgggga gaaggactgc gagggtggag cggatgaggc cggctgtgct accttgtgcg
      661 ccccgcacga gttccagtgc ggcaaccgct cgtgcctggc cgccgtgttc gtgtgcgacg
      721 gcgacgacga ctgtggtgac ggcagcgatg agcgcggctg tgcagacccg gcctgcgggc
      781 cccgcgagtt ccgctgcggc ggcgatggcg gcggcgcctg catcccggag cgctgggtct
      841 gcgaccgcca gtttgactgc gaggaccgct cggacgaggc agccgagctc tgcggccgtc
      901 cgggccccgg ggccacgtcc gcgcccgccg cctgcgccac cgcctcccag ttcgcctgcc
      961 gcagcggcga gtgcgtgcac ctgggctggc gctgcgacgg cgaccgcgac tgcaaagaca
     1021 aatcggacga ggccgactgc ccactgggca cctgccgtgg ggacgagttc cagtgtgggg
     1081 atgggacatg tgtccttgca atcaagcact gcaaccagga gcaggactgt ccagatggga
     1141 gtgatgaagc tggctgccta caggggctga acgagtgtct gcacaacaat ggcggctgct
     1201 cacacatctg cactgacctc aagattggct ttgaatgcac gtgcccagca ggcttccagc
     1261 tcctggacca gaagacctgt ggcgacattg atgagtgcaa ggacccagat gcctgcagcc
     1321 agatctgtgt caattacaag ggctatttta agtgtgagtg ctaccctggc tacgagatgg
     1381 acctactgac caagaactgc aaggctgctg ctggcaagag cccatcccta atcttcacca
     1441 accggcacga ggtgcggagg atcgacctgg tgaagcggaa ctattcacgc ctcatcccca
     1501 tgctcaagaa tgtcgtggca ctagatgtgg aagttgccac caatcgcatc tactggtgtg
     1561 acctctccta ccgtaagatc tatagcgcct acatggacaa ggccagtgac ccgaaagagc
     1621 aggaggtcct cattgacgag cagttgcact ctccagaggg cctggcagtg gactgggtcc
     1681 acaagcacat ctactggact gactcgggca ataagaccat ctcagtggcc acagttgatg
     1741 gtggccgccg acgcactctc ttcagccgta acctcagtga accccgggcc atcgctgttg
     1801 accccctgcg agggttcatg tattggtctg actgggggga ccaggccaag attgagaaat
     1861 ctgggctcaa cggtgtggac cggcaaacac tggtgtcaga caatattgaa tggcccaacg
     1921 gaatcaccct ggatctgctg agccagcgct tgtactgggt agactccaag ctacaccaac
     1981 tgtccagcat tgacttcagt ggaggcaaca gaaagacgct gatctcctcc actgacttcc
     2041 tgagccaccc ttttgggata gctgtgtttg aggacaaggt gttctggaca gacctggaga
     2101 acgaggccat tttcagtgca aatcggctca atggcctgga aatctccatc ctggctgaga
     2161 acctcaacaa cccacatgac attgtcatct tccatgagct gaagcagcca agagctccag
     2221 atgcctgtga gctgagtgtc cagcctaatg gaggctgtga atacctgtgc cttcctgctc
     2281 ctcagatctc cagccactct cccaagtaca catgtgcctg tcctgacaca atgtggctgg
     2341 gtccagacat gaagaggtgc taccgagcac ctcaatctac ctcaactacg acgttagctt
     2401 ctaccatgac gaggacagta cctgccacca caagagcccc cgggaccacc gtccacagat
     2461 ccacctacca gaaccacagc acagagacac caagcctgac agctgcagtc ccaagctcag
     2521 ttagtgtccc cagggctccc agcatcagcc cgtctaccct aagccctgca accagcaacc
     2581 actcccagca ctatgcaaat gaagacagta agatgggctc aacagtcact gccgctgtta
     2641 tcgggatcat cgtgcccata gtggtgatag ccctcctgtg catgagtgga tacctgatct
     2701 ggagaaactg gaagcggaag aacaccaaaa gcatgaattt tgacaaccca gtctacagga
     2761 aaacaacaga agaagaagac gaagatgagc tccatatagg gagaactgct cagattggcc
     2821 atgtctatcc tgcagcaatc agcagctttg atcgcccact gtgggcagag ccctgtcttg
     2881 gggagaccag agaaccggaa gacccagccc ctgccctcaa ggagcttttt gtcttgccgg
     2941 gggaaccaag gtcacagctg caccaactcc cgaagaaccc tctttccgag ctgcctgtcg
     3001 tcaaatccaa gcgagtggca ttaagccttg aagatgatgg actaccctga ggatgggatc
     3061 acccccttcg tgcctcatgg aattcagtcc catgcactac actctggatg gtgtatgact
     3121 ggatgaatgg gtttctatat atgggtctgt gtgagtgtat gtgtgtgtgt gatttttttt
     3181 ttaaatttat gttgcggaaa ggtaaccaca aagttatgat gaactgcaaa catccaaagg
     3241 atgtgagagt ttttctatgt ataatgtttt atacactttt taactggttg cactacccat
     3301 gaggaattcg tggaatggct actgctgact aacatgatgc acataaccaa atgggggcca
     3361 atggcacagt accttactca tcatttaaaa actatattta cagaagatgt ttggttgctg
     3421 ggggggcttt tttaggtttt ggggcatttg ttttttgtaa ataagatgat tatgctttgt
     3481 ggctatccat caacataagt aaaaaaaaaa aaaaaacact tcaactccct cccccattta
     3541 gattatttat taacatattt taaaaatcag atgagttcta taaataattt agagaagtga
     3601 gagtatttat ttttggcatg tttggcccac cacacagact ctgtgtgtgt atgtgtgtgt
     3661 ttatatgtgt atgtgtgtga cagaaaaatc tgtagagaag aggcacatct atggctactg
     3721 ttcaaataca taaagataaa tttattttca cacagtccac aaggggtata tcttgtagtt
     3781 ttcagaaaag cctttggaaa tctggatcag aaaatagata ccatggtttg tgcaattatg
     3841 tagtaaaaaa ggcaaatctt ttcacctctg gctattcctg agaccccagg aagtcaggaa
     3901 aagcctttca gctcacccat ggctgctgtg actcctacca gggctttctt ggctttggcg
     3961 aaggtcagtg tacagacatt ccatggtacc agagtgctca gaaactcaag ataggatatg
     4021 cctcaccctc agctactcct tgttttaaag ttcagctctt tgagtaactt cttcaatttc
     4081 tttcaggaca cttgggttga attcagtaag tttcctctga agcaccctga agggtgccat
     4141 ccttacagag ctaagtggag acgtttccag atcagcccaa gtttactata gagactggcc
     4201 caggcactga atgtctagga catgctgtgg atgaagataa agatggtgga ataggtttta
     4261 tcacatctct tatttctctt ttccccttac tctctaccat ttcctttatg tggggaaaca
     4321 ttttaaggta ataaataggt tacttaccat catatgttca tatagatgaa actaattttt
     4381 ggcttaagtc agaacaactg gccaaaattg aagtcatatt tgagggggga aatggcatac
     4441 gcaatattat attatattgg atatttatgt tcacacagga atttggttta ctgctttgta
     4501 aataaaagga aaaactccgg gtatatgtat agatgttctt cattatagac atcttctttg
     4561 cttttcttgg ccttggggga ggaagggaga agtgctcttt tctacttgtg gggtctccca
     4621 ttggaaacat aatcctatag tcccagaagg attcagtccc cagtggcttt cccatccaaa
     4681 gagaaagagt ttgagtttct taactctgct gttctgccac ttactcccac tagacaacca
     4741 gggacaaggt gcaacatgga agtgtttgac ttaagtagga gcagaggagc tgcatctaat
     4801 ctcatcatac ctggaacttg acacacttaa gcaaatgcct tcccatccct acctgccaga
     4861 tgcccccaac tcaatgaagt tggatgtctc accagcttga taccctttga attttcagtc
     4921 agacattctg gagttctagc atcctgtacc taggaccttc ctctgtgtca ctcttggcct
     4981 cctaaactct aagaaaataa ctatattctg gagcttgggc agtgtgtttt gcataatcca
     5041 gcaatctcct catgacatgc atgtgttgat agtcctgaaa cattcattga gagggtaaat
     5101 gcagttgacc tagaatgacc aataccaaac agaattttaa gaacaggtgg ccaactccta
     5161 tggagcttac tcacatatta ctattctttt aagaacggaa agtaaaatta tttttgactg
     5221 aagaaaaatg atgacagtga aaaacatgga aatgtactca aaacaagtga ctttttctgt
     5281 aaccttccaa agaaactgaa ttttccaagg aattaaatga taacagtggc taaggcatag
     5341 tttctaaact ttcagtaaga tcctggcatt cacagaaaaa aatgatgaat ggggtctgga
     5401 catacagcct gagatctcaa aatgacaatg aaattcacaa ctttttctca gagacattca
     5461 tgtttcctgc atatgctaca actgcagttt gaaagaggca gcaatgggag caacccttta
     5521 caagaaacaa attgtgatat attcatgtgt tggacggcag taaataagat gaaacctgag
     5581 gagtcagatc caccttcccc cattcataga ggcttttcag cctcattttg aggtacagtt
     5641 acatatcttt tgccttttgc ccccgtgcat agctatctac agccaatcac agatcacaga
     5701 gtcactggac tatagagctg gaaggaagct cagagacaat gccaaggggg cagaaaattt
     5761 atcagaagcc agtcccagtg cgtttcctcc atttccttct gcaggaagac tattttgggc
     5821 tgcctgaaca ttgtatcaaa cctgctacct atactatggt ctacctttcc tccagtggaa
     5881 ttacaaaggc actaactgaa atgccttcta gaaacagaga aaacgaaact gtacttattt
     5941 actcttgata cacagattat ttataaaaca gattgaagta acctgttaac tggcaaaaag
     6001 agaatgagat cggatttaaa tgtatggcag taagtcctat tgatccctcc agttatctca
     6061 gtatgactgc agtatattca ttcactaaaa ccactcacta gataccaact acacacctgg
     6121 cactgcagat gtaaaggtca gtcacacatg ttctgacttt acagagttca cagtagcagt
     6181 ggaggatgat atatgtggaa acaaaaaagg cattgattct attcagagca ctgttagggc
     6241 tcaaaggaga gaggggtctt tccacctaag aaatgaggaa tagggtcatc atagaagtga
     6301 ccttaagtct taaaaattaa gaaggggatt ccaagctgct tcagacagag acacatcgag
     6361 ctaaaacaca gaggtatgaa agagcacagg gactttagga attgcacagt tcattctaac
     6421 aggaacaaaa ggctcaaggg gggcaagaaa tgaggctgta tggaaagaga ttcaatgtaa
     6481 gcactttata aaatagatta atttctgatt caatgaagca tttcttgatc attgtgtaca
     6541 aggcactaca tgcatcatgg aaaattcatt aggatgcatt gccagcactt tgcagaactg
     6601 atattattca gcctcaagct ttccagtggc caaagggaaa tgctgactgc ttttcatata
     6661 tttgagtcaa agatttttta tatggtcaat gaagactaat ataagggcag tgggattttc
     6721 acagatgcat gccatgttgt cgagagcctc ttagattttc tcaactgtga gaaagaaaaa
     6781 cgaaaatgtt gaagacgttg agtctggaga ggggatacta atcactgtcc agttgggcac
     6841 tggtgggaat ggggaaatgg cacaggaatg caagcctctc caccctaccc cccgaactcc
     6901 agccatacac tcatcgtttc acaaaatata aatgagttag cattaaatgt ttcagagtaa
     6961 ataattcctt ttcccgaaat gcatgaagat agagtaacag acttctcaca ctgtattttt
     7021 agggtatgga gaatttagaa ggttaaagaa ttactgcttc aatttttcag ttaaaaaaaa
     7081 atcaggaagc tctgttcatt caggctatgc accatgtgca cagtcaagaa ttagcagaaa
     7141 ccctctgcat ttacaaacac tttgtgctat aaaaaagtaa tttttaaaaa gccacgtgtg
     7201 tgtgtgtgta tatatatata tatatatata tttaaagcca aggttttgat acttttttac
     7261 aaaaactaca agagaaaaca aatatacctg tccaaaccat atacttttaa aagagcattt
     7321 ttttttccat acaagctgtt gttaatttgg gggtaaagtg ctgatttgca aacttcatca
     7381 aattgttccc aagtggattc tccttgtttg tctcccccta ccaaccccaa agttaccata
     7441 tttgatgtaa gaatcaggca tgttagaatg ttgtgtcaca ctaactgatt ctgctctttt
     7501 tgtcttgtca ttcaagttcc gttagcttct gtacgcggtg ccctttgcag tctggtgtct
     7561 cttccagagg cgagggggct gaggatgggg tgctgcatct cactagctat actggcatca
     7621 tcttggtaaa ctgaaaacca aatgtggaca tttgtaaaat cagtgcactg tttctagaga
     7681 gagattaaat tcatttaaaa aaaa
//
