LOCUS       NM_014813              11566 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens leucine rich repeats and immunoglobulin like domains 2
            (LRIG2), transcript variant 1, mRNA.
ACCESSION   NM_014813 XM_375770
VERSION     NM_014813.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Hall,A., Middlehurst,B., Cadogan,M.A.M., Reed,X., Billingsley,K.J.,
            Bubb,V.J. and Quinn,J.P.
  TITLE     A SINE-VNTR-Alu at the LRIG2 locus is associated with proximal and
            distal gene expression in CRISPR and population models
  JOURNAL   Sci Rep 14 (1), 792 (2024)
   PUBMED   38191889
  REMARK    GeneRIF: A SINE-VNTR-Alu at the LRIG2 locus is associated with
            proximal and distal gene expression in CRISPR and population
            models.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Li,Z.Q., Liao,W.J., Sun,B.L., Luo,Z.W., Zhong,N.S., Wu,J.B.,
            Liu,Z.L. and Liu,J.M.
  TITLE     LRIG2 regulates cell proliferation, migration and apoptosis of
            osteosarcoma
  JOURNAL   BMC Cancer 22 (1), 1029 (2022)
   PUBMED   36183058
  REMARK    GeneRIF: LRIG2 regulates cell proliferation, migration and
            apoptosis of osteosarcoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11566)
  AUTHORS   M Serag El-Dien,M., Fathy Mahmoud,S., Alhanafy,A.M., Mohamed
            Zanaty,F. and Shawky Holah,N.
  TITLE     Prognostic significance of LRIG2 and LRIG3 proteins in urothelial
            bladder carcinoma
  JOURNAL   J Immunoassay Immunochem 43 (3), 308-332 (2022)
   PUBMED   34839782
  REMARK    GeneRIF: Prognostic significance of LRIG2 and LRIG3 proteins in
            urothelial bladder carcinoma.
REFERENCE   4  (bases 1 to 11566)
  AUTHORS   Herdenberg,C., Mutie,P.M., Billing,O., Abdullah,A.,
            Strawbridge,R.J., Dahlman,I., Tuck,S., Holmlund,C., Arner,P.,
            Henriksson,R., Franks,P.W. and Hedman,H.
  TITLE     LRIG proteins regulate lipid metabolism via BMP signaling and
            affect the risk of type 2 diabetes
  JOURNAL   Commun Biol 4 (1), 90 (2021)
   PUBMED   33469151
  REMARK    GeneRIF: LRIG proteins regulate lipid metabolism via BMP signaling
            and affect the risk of type 2 diabetes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11566)
  AUTHORS   Stuart,H.M., Roberts,N.A., Burgu,B., Daly,S.B., Urquhart,J.E.,
            Bhaskar,S., Dickerson,J.E., Mermerkaya,M., Silay,M.S., Lewis,M.A.,
            Olondriz,M.B., Gener,B., Beetz,C., Varga,R.E., Gulpinar,O.,
            Suer,E., Soygur,T., Ozcakar,Z.B., Yalcinkaya,F., Kavaz,A.,
            Bulum,B., Gucuk,A., Yue,W.W., Erdogan,F., Berry,A., Hanley,N.A.,
            McKenzie,E.A., Hilton,E.N., Woolf,A.S. and Newman,W.G.
  TITLE     LRIG2 mutations cause urofacial syndrome
  JOURNAL   Am J Hum Genet 92 (2), 259-264 (2013)
   PUBMED   23313374
  REMARK    GeneRIF: A subset of urofacial syndrome-affected individuals have
            biallelic mutations in LRIG2
REFERENCE   6  (bases 1 to 11566)
  AUTHORS   Karlsson,T., Mark,E.B., Henriksson,R. and Hedman,H.
  TITLE     Redistribution of LRIG proteins in psoriasis
  JOURNAL   J Invest Dermatol 128 (5), 1192-1195 (2008)
   PUBMED   18037903
  REMARK    GeneRIF: LRIG proteins may have a role in epidermal homeostasis and
            psoriasis
REFERENCE   7  (bases 1 to 11566)
  AUTHORS   Guo,D., Han,L., Shu,K., Chen,J. and Lei,T.
  TITLE     Down-regulation of leucine-rich repeats and immunoglobulin-like
            domain proteins (LRIG1-3) in HP75 pituitary adenoma cell line
  JOURNAL   J Huazhong Univ Sci Technolog Med Sci 27 (1), 91-94 (2007)
   PUBMED   17393120
REFERENCE   8  (bases 1 to 11566)
  AUTHORS   Guo,D., Holmlund,C., Henriksson,R. and Hedman,H.
  TITLE     The LRIG gene family has three vertebrate paralogs widely expressed
            in human and mouse tissues and a homolog in Ascidiacea
  JOURNAL   Genomics 84 (1), 157-165 (2004)
   PUBMED   15203213
REFERENCE   9  (bases 1 to 11566)
  AUTHORS   Holmlund,C., Nilsson,J., Guo,D., Starefeldt,A., Golovleva,I.,
            Henriksson,R. and Hedman,H.
  TITLE     Characterization and tissue-specific expression of human LRIG2
  JOURNAL   Gene 332, 35-43 (2004)
   PUBMED   15145052
  REMARK    GeneRIF: LRIG2 was found to be a glycoprotein with an molecular
            weight of 132 kDa, which was encoded by a gene on human chromosome
            1p13 and its mRNA was present in all tissues analyzed.
REFERENCE   10 (bases 1 to 11566)
  AUTHORS   Newman,W.G., Woolf,A.S., Beaman,G.M. and Roberts,N.A.
  TITLE     Urofacial Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23967498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291031.1, AL357055.18 and
            CR936669.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_014813.2.
            
            Summary: This gene encodes a transmembrane protein containing
            leucine-rich repeats and immunoglobulin-like domains. The encoded
            protein promotes epidermal growth factor signalling, resulting in
            increased proliferation. Its expression in the cytoplasm of glioma
            cells is correlated with poor survival. Mutations in this gene can
            cause urofacial syndrome. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.222045.1,
                                           SRR1660803.260442.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361127.6/ ENSP00000355396.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-954               AK291031.1         21-974
            955-955             AL357055.18        67092-67092
            956-2332            AK291031.1         976-2352
            2333-2333           AL357055.18        86864-86864
            2334-3639           AK291031.1         2354-3659
            3640-4508           CR936669.1         3771-4639
            4509-11566          AL357055.18        97597-104654
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..11566
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /note="leucine rich repeats and immunoglobulin like
                     domains 2"
                     /db_xref="GeneID:9860"
                     /db_xref="HGNC:HGNC:20889"
                     /db_xref="MIM:608869"
     exon            1..448
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..3407
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; leucine-rich repeats and immunoglobulin-like
                     domains protein 2"
                     /codon_start=1
                     /product="leucine-rich repeats and immunoglobulin-like
                     domains protein 2 isoform 1 precursor"
                     /protein_id="NP_055628.1"
                     /db_xref="CCDS:CCDS30808.1"
                     /db_xref="GeneID:9860"
                     /db_xref="HGNC:HGNC:20889"
                     /db_xref="MIM:608869"
                     /translation="MAPAPLGVPEEQLLGCRSRVLSRLLFIAQTALLLLPAAGAGLCP
                     APCSCRIPLLDCSRRKLPAPSWRALSGLLPPDTAILDFSHNRLSNWNISLESQTLQEV
                     KMNYNELTEIPYFGEPTSNITLLSLVHNIIPEINAQALQFYPALESLDLSSNIISEIK
                     TSSFPRMQLKYLNLSNNRITTLEAGCFDNLSSSLLVVKLNRNRMSMIPPKIFKLPHLQ
                     FLELKRNRIKIVEGLTFQGLDSLRSLKMQRNGISKLKDGAFFGLNNMEELELEHNNLT
                     RVNKGWLYGLRMLQQLYVSQNAIERISPDAWEFCQRLSELDLSYNQLTRLDESAFVGL
                     SLLERLNLGDNRVTHIADGVFRFLSNLQTLDLRNNEISWAIEDASEAFAGLTSLTKLI
                     LQGNQIKSITKKAFIGLESLEHLDLNNNAIMSIQENAFSQTHLKELILNTSSLLCDCH
                     LKWLLQWLVDNNFQHSVNVSCAHPEWLAGQSILNVDLKDFVCDDFLKPQIRTHPETII
                     ALRGMNVTLTCTAVSSSDSPMSTVWRKDSEILYDVDTENFVRYWQQAGEALEYTSILH
                     LFNVNFTDEGKYQCIVTNHFGSNYSQKAKLTVNEMPSFLKTPMDLTIRTGAMARLECA
                     AEGHPAPQISWQKDGGTDFPAARERRMHVMPEDDVFFIANVKIEDMGIYSCMAQNTAG
                     GLSANASLTVLETPSFIRPLEDKTVTRGETAVLQCIAGGSPAPRLNWTKDDGPLLVTE
                     RHFFAAANQLLIIVDAGLEDAGKYTCIMSNTLGTERGHIYLNVISSPNCDSSQSSIGH
                     EDDGWTTVGIVIIVVVCCVVGTSLIWVIVIYHMRRKNEDYSITNTEELNLPADIPSYL
                     SSQGTLSEPQEGYSNSEAGSHQQLMPPANGYIHKGTDGGTGTRVICSDCYDNANIYSR
                     TREYCPYTYIAEEDVLDQTLSSLMVQMPKETYLVHPPQDTTALESLIPSANREPSAFP
                     TNHERISEKKLPSTQMSGETLQRPVWNINRELGLPHPPFSQQPVHESPQLHQNEGLAG
                     REPDCSASSMSCHRLQDHAFDFSRTRNIQDGSEGT"
     exon            449..514
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            515..589
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            590..724
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            725..868
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1012
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1156
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1300
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1381
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1453
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1522
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1686
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..2007
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2289
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2739
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2889
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..3180
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3181..11566
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11545..11550
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /note="hexamer: AATAAA"
     polyA_site      11566
                     /gene="LRIG2"
                     /gene_synonym="LIG-2; LIG2; UFS2"
                     /note="major polyA site"
ORIGIN      
        1 ggacgagagg tgtccgtcag gccgtgtgtc ccaggccgtc gaccccgctg tcgcgctgcg
       61 tctgctcctt gcatgccttt agatggtaca gccttcagcc ggggcttcgg gagacagtgc
      121 agccaccgag catctctgct gagcttctcc gccgatcctc cttttctagc aggcagctct
      181 tctaggccac gtccaggtcg agggggaaaa tggcgccggc gcccctaggc gtcccggagg
      241 agcagttgct ggggtgtcga tctagagtgc tttctcggtt actcttcatt gcccagaccg
      301 ctctcctcct gttgcccgcc gccggagcag gtctctgccc cgcgccctgc tcctgccgca
      361 ttcctctcct ggactgcagt cgcaggaaat tgcccgcacc gagctggagg gcgctgtcgg
      421 gcttgctgcc ccccgacacc gctatcctgg atttcagtca taatcggttg tctaactgga
      481 acatcagctt ggaatcacaa acattacagg aagtgaaaat gaattacaat gaactaacag
      541 aaatcccgta ttttggagaa cctacatcta atattactct actttcatta gtccataata
      601 taatcccaga aataaatgca caggcactcc agttttaccc tgctctggag agtttagacc
      661 tcagctcaaa tataatatca gaaatcaaga catcttcatt tcctcgcatg cagcttaaat
      721 acctgaattt aagtaataac agaataacca ccttggaggc tggttgcttc gataatttat
      781 caagttcctt attagtggta aagttaaacc gtaaccgaat gagcatgatt ccacctaaga
      841 tcttcaagct gcctcacctc caattcttgg aacttaaaag aaacagaatt aaaattgtgg
      901 aaggtcttac attccaaggg cttgactcct taagatcttt gaaaatgcag cggaatggaa
      961 ttagcaaact taaggatgga gcattttttg gcttgaataa catggaagaa ctagaactgg
     1021 aacacaacaa ccttacacga gtaaacaagg ggtggttgta tggcttgcga atgttacagc
     1081 agctctatgt gagccagaat gctattgaaa gaatcagccc tgatgcatgg gagttctgcc
     1141 aaagactatc cgaacttgat ttgtcctata accagctgac ccgcctggat gaatctgcct
     1201 ttgtgggtct gagcttattg gagagattga atttaggaga caacagagtc actcatattg
     1261 ctgatggtgt atttagattt ctttccaatc ttcagacatt agacttaaga aacaatgaaa
     1321 tttcatgggc catagaagat gctagtgaag cctttgctgg actcacaagt ctcactaaac
     1381 taatcttaca aggaaaccag attaagtcaa ttacaaagaa agcattcatt ggtcttgaat
     1441 cccttgagca tctagatttg aacaataatg ctataatgtc tatccaagaa aatgcttttt
     1501 ctcagactca tttaaaagaa ctgattctga acacaagcag tttgctctgt gactgccatt
     1561 tgaagtggct acttcaatgg ttggttgata ataactttca acattctgtg aatgtaagct
     1621 gtgcacaccc tgaatggcta gcagggcaaa gcatcctgaa tgtggatctg aaagattttg
     1681 tctgtgatga ttttctcaag ccacagataa ggacacatcc tgaaaccata attgctctaa
     1741 gaggcatgaa tgtgactctg acgtgcactg cagtgagcag cagtgattca cccatgtcca
     1801 ctgtgtggcg caaagacagt gaaatcctgt atgacgtgga tactgagaat tttgttcgtt
     1861 attggcagca agctggagaa gctctggaat atactagtat cttacatctt ttcaatgtga
     1921 atttcacaga tgaaggaaaa tatcagtgta ttgttactaa tcactttggt tctaattatt
     1981 ctcagaaagc caaactgact gtaaatgaga tgccatcttt tctgaaaacg ccaatggatc
     2041 tgactattcg cactggtgcc atggccagat tagaatgtgc tgcagaggga caccctgcac
     2101 ctcagatttc ctggcagaaa gatggtggta ctgactttcc tgcggctcga gaaagacgca
     2161 tgcacgtcat gcccgaggat gacgtcttct ttattgccaa tgtgaaaata gaagatatgg
     2221 gaatctatag ctgcatggca caaaatacag caggaggtct ctcagcaaat gcttccctaa
     2281 cagtgttaga gacaccctca tttattagac ccctggagga taagacagta acacgaggtg
     2341 aaactgcggt gttacagtgc atagctggag ggagtcctgc ccctcgtctc aactggacta
     2401 aagatgatgg gcctttgctg gtgacagaac gacatttctt tgctgcagcc aatcagcttc
     2461 tcatcattgt agatgccggg ctagaagatg ctgggaaata tacctgcatt atgtctaaca
     2521 cccttgggac agaacgtggc cacatttacc taaatgtcat ttcatccccc aattgtgact
     2581 cttcccagag tagcattggg catgaagatg atggctggac cacagttggc attgtcatca
     2641 ttgttgtggt ctgctgtgtt gttggcactt ctttgatctg ggtcattgtt atttaccaca
     2701 tgagaaggaa aaatgaagac tatagtatca caaacacaga ggagctcaat ctgcctgcag
     2761 acattcccag ctacttgtct tcccaaggaa cgctgtctga gccacaggaa ggctacagca
     2821 actctgaggc aggcagtcat cagcaactta tgcctcctgc caatggatat atacacaaag
     2881 gcactgacgg tggcactggt acccgggtga tttgctcaga ttgttatgac aatgccaaca
     2941 tctactccag gacccgagaa tactgtccat acacctatat tgctgaggag gacgttcttg
     3001 atcagacact gtccagcctc atggtccaaa tgcctaaaga gacatattta gtacatcctc
     3061 cccaggatac tactgcccta gagagcctga taccgtcagc caacagagag ccatctgcct
     3121 ttcccaccaa ccatgagagg ataagtgaga agaaacttcc ctccacacag atgagcggtg
     3181 aaacattgca gcggcccgtg tggaacataa acagagaact aggcctgcct catcctcctt
     3241 tttcccagca gccagtccat gagtcaccac aacttcatca aaatgagggc ctggcaggga
     3301 gagagccaga ctgttctgct tcttccatgt cctgccacag gttacaggat catgcttttg
     3361 attttagtag gactcggaac attcaagatg gtagtgaggg cacatgaaac tcacttcagg
     3421 atgaaatctg ggcagagact tattaattaa ttttgcattt actacctcag agctcagaag
     3481 aaactccgaa gtcagcattt gctttactct ttctttatga ttgcatctga ccgcaccaag
     3541 gtgggccatg cgttgtttgg tcttatacct gatgaagaaa tggcaacagc tgacagaaat
     3601 gggtacagct catcaaaaat gtgcagcacc ggcagaggga agatacgggg caaatgtgct
     3661 tcctgatgct tccatgggga tgtgccctgg tgtgcatctg cttgtcagga agagtcacat
     3721 tgctgcttaa catgctggat tgccctagtc ttcagaatgg tcctgagaaa acatcactac
     3781 ttcgatgttc tactttgctt tccaaggagc aaaaataact ttggagcctt ctgggaagtg
     3841 tgcctgggat tcttcagtgg tttcaggcag atagttgaga ctggggcttt gatattcaag
     3901 gtctttggca agaatcccag gcttgaccaa ctggtaccag gtcaaagatt tttgtattct
     3961 tgtgaatttt tttttttgtc cctattgccc agggatgtca ttgtaaatat atgtgcattt
     4021 ataaataatt tttgtattca ttgaccatat gtgttggctg caatgtaaat atttttgata
     4081 tgccaaaatt atatgtaata tccagttata atattgacaa gtagcttctc agatcacaga
     4141 ttttaaataa ctcaatttag tgagatctaa aaattttatt taaagtataa aaactgtggc
     4201 tcacacctgt aatcccagca ttttgagagg ctaaggtggg aggattgctt gaggccagga
     4261 atttgagacc aggctgggca acatggtgag accctgtgtc tgcaaaaaaa taaattaaaa
     4321 aaataggtgg acattgtggc atgctgctgt agtcctagct acttgggagg ctgaggtggg
     4381 agggtcactt gagcccggga aatcaaggct gcagtgagct gtgtgaagcc actgcatccc
     4441 agcctgggtg acagagtgag gccctggctc aaaagtaaaa taaataaata aaaagtttta
     4501 aaaacttact tgttaagaag tgtatctata gaggcagcta cttagtgatt gtatacacat
     4561 aaacacagtc ttggtgtaat taaccgcctt cttcatgcct agaggttcat acttctggtc
     4621 atttgccatc tgtttaaaag tgctaggtga tactcccttt ctaacataaa aagcttgttc
     4681 tttcagagcc tggcagtcct gtcacagctg tatgcaccta gaggaaaact tgtttggaaa
     4741 ttcctttcat ttcctccccc tctccctcct tccctctttt tttttactga aacagaaatt
     4801 gattgaggaa ggttcagtca ttccttcagg aaccaatgag ttagatcggg ttgttacttt
     4861 atgtataatg aacgcttcaa aaactggagg ctgatttaaa gattgcagaa cttgagtcag
     4921 actcataagc agtactgtga catgaattgg agccagaaca gttgctgata tcagaaccat
     4981 ctctttcatt ctgtgacttt gctcttgcca tgttctgact ggggtgagcc gctaggatac
     5041 aaatcactat cactgtccat atttgagtct gcaaatcttg tcattgtctg ctttaatgct
     5101 ctttgagttg caacaggtgt attatttaaa tgagtatttg aaattctgtg aacaggaatt
     5161 cttttggtag tgaaataggt gctcacacct gcttgctcag aatgtgaaaa tattatttct
     5221 gagttctctt tgttcacttt ctaaagtgac tgttgtttgg tgttggagat gttaactggg
     5281 ttctattagg tgtgctatat cttggcaaat attccccatg atgaactctt cctactatca
     5341 caatcaggac tatgactgga tttctcagtc tcagtgcccc agattcacca tttctgattg
     5401 tgtctggaga acgttttcct ggaaaggcat tccatgttcc aaaagctaca aaaacatgcc
     5461 actttgcaac tatgggtcag ttatttgctc tctcaatgtc tcatgttgct gagttctaga
     5521 aaatctctgt ctcatttacc aggggtcatt caaaagtctg agatgtatat tatcatgaaa
     5581 tatttgtgaa atttctcacc atgaatttgg agggaaccta cttttatttc tcgctctggt
     5641 tctccattgg tctgggctcc tgcttctcag tcttctctga cctctggtaa gtggaacttt
     5701 cattaatact tctgtcgcac tttgtacctg acctaggtgg ccttgcaaat gttgactcag
     5761 attgtggatt ttccactttc ctctgttttc cctattttct cccctgggaa atcttgcaga
     5821 tgatgcatat tcattttctg tattcagcct tccattatta aggataaaat tgatccctac
     5881 attgaatctg aaattaccac tatatggatt actctggctc actgctgtcc aagcttggtt
     5941 ttctctttgc ttacttcctg tcatattttc agtttgccca aaagaggtaa gccactttat
     6001 tacagaccca ttactagtgc tagacgtcat ccacctctgt tttcacctga ttcttgtggg
     6061 gaatcctctt taatttgttg taaccttctc agcaactctt ctgtacttca cctatagtgc
     6121 ctagcaaatt gttatcactc ataagcctgt aatcttcatc actcaggtta tttgcaagta
     6181 gaaagcttct ggaaccattt gtccatccga cttctgcact gggccacatc catcttcttc
     6241 attgtcagaa tctgagtttt ctgtcttgaa gaacagctgg aaagttgtct ggctgttatg
     6301 acacagtatt gaaataccag actgagtttc agttgtttct agttgtgttc actttgtgct
     6361 ttctctgaat gggaatatct tctttcagca aagttcttca ttgaccagga aaccaaagat
     6421 tgcttctttt ccaagtttgt tttgttttta atcttttacc aatgtgcagt atatcttaag
     6481 aagggaagag tgctcttggg agtagaagaa catgcaaaca ttcttttttc tttattatta
     6541 tttgagatgg agtccctctc ggtcacccag gctggagcgc agtggcatga tcttggctta
     6601 ctgcaacctc cgcctcccag gttcaagcaa ttctcttgcc tcagcctccc aagtagctgg
     6661 gattacaggc acaccaccat ttccagcttt tttttttttt ttttttttga tatttttagt
     6721 agagatgggg tttcaccatg ttggccaggc tggtcttgaa ctcctgacct caggtgatct
     6781 gcttgccttg gcctcccaaa gtgctgggat tacaggcatg agccacttgc ctggccccaa
     6841 aattcttata ctgtaaagcc ttgtagtggt ccttattttt ttttttttga gacggagtct
     6901 cgctctgtca cccagactgg agtgcagtgg cacaatctcg gctcactgca acctctgcct
     6961 cccgagttca agcccttctt ctgcctcagc ctcccaagta cctgggatta caggcatgca
     7021 ccaccatgcc cggctaattt tatttgtatt tttagtagag acggggtttc tccatgttgg
     7081 tcaggctggc ctcgaactcc tgacctcagg tgatccaccc gcctcggcct cctagagtgc
     7141 tgggattaca ggcgtgagcc accgagcccg gccggtcctt attttataca agtgattaag
     7201 ccaaaaattt tcttgaattt ctgataccaa agagctttag gtaaataact tcacactcta
     7261 atgcaatgta attctttctt tgtgctttca gatttcctta cacttcctgt ccagttctct
     7321 cttctaaatc caatattact cactagataa tttcagccac ttaaataaaa atatctattt
     7381 ttaagtcctg gttcaagggt tccaaactca cataatttct caactttttg ccagaaagtt
     7441 gagcatttat tcatacttat tttcaatcct aagtgttctc ccatgggcag cagcacttct
     7501 tgagctgttt agtactgcca caccttttta tgtctttact atgtcagcct ctgatcattt
     7561 tcactttgaa cttcctaaag atcacaattt cctatccctt caatctgcga tccctcttct
     7621 tcctttctta ctagattatg gtgtagtttg tactagactg actcattaag accttgtacg
     7681 cttcctgagt ctgcttatta ctggctttgt aatcaagggt gagtcttttt acttctctag
     7741 gctagatttt ctcatttatg aaatgaggac atttttctag atgctgtaaa gccctttcca
     7801 gctgtaacat tctatgaatc tgtaattccc tcgaggagct aaggaggatt atgctacttt
     7861 aataaaaagc tgctttggtg acatttgttc gagtagaaca agagtgatgc tgctcattct
     7921 gacttgagcc agatgtattt cctttatagc aggagtaatg gtctaagaaa tactcattag
     7981 gagaaaaaaa ttctgcttct aaacagtatt agtaaacaaa gaatcttgac tcagttgaaa
     8041 aaccacccat tgctctctag agcagaatgt ctgaatgctg gttttatagc acactagaat
     8101 atcagaatga gttgacaggt ataccacaac taatttgtaa ctagtttgtg actcaccttt
     8161 ctaatgtatt cgaagttatc cctataatta tattactctc gctcaaattc tgatagattt
     8221 cctgaatgga ataggtggaa ttagggctag gctgccagag actgtgccaa tgagcatcac
     8281 ccgtcatgtg tgagagagca gaagatagcc tgtgagccag tgctctagaa aacatcatgt
     8341 aggaggccgg tcacggtggc tcacgcctgt aatcccagca ctttgggagg ccgaggtgga
     8401 tggatcaaga gatcgagacc atcctggcca acatggtgaa attccacctc tactaaaaat
     8461 acaaaaatta gctgggcgtg gtggtgcgcg cctgtagtcc tagctgctcg ggaggctaag
     8521 gcaggagaat cacttgaacc tgggaggcag aggttgcagt gagccgagat tgcaccactg
     8581 cactccagcc tggtgacaga gtgagactcc gtctcaaaaa aaaaaaaaaa aaaacccgtg
     8641 taggagtact gcctctctgc tctagcagtg gtccttctac tgacagtgct gtcagagtct
     8701 ggtctcctaa agagccaggt cattttccat tgtgtcaact cagatggttg agatgtcctt
     8761 agatattcct agcttgccta aataactagt cagcaatcta tttaaatgtg aagagcctat
     8821 ctctggtctt taaataaaca cacacacaca cacacacccc taggaaaagg aattcctgaa
     8881 acttttcatt attgcatatg tttctgatca agcaatatgt taaccctcta agtaggatta
     8941 gtgtcccatg agtatttctt ccagcagaat catgtctgcc taatgctaat caggaatgtt
     9001 gcaaactgaa atgctccttt ccccactgcc ccctaatgac cactttcagg aggaatacat
     9061 acattctctt gatttgtttg tcccctttca ggctgattct ccacttccac ctggaagaaa
     9121 agacagtatt ccttgaactc catgacctac tggtacagga tctacactta tgccattggc
     9181 tttctgtcac tccgcaactt gtactttttt ttttgagaca gggtttcacc ctgtcagcca
     9241 ggctggagtg caatggtgtg aacgtggctc actgtcgtca ctgcagcctt gatatctcaa
     9301 gcccaagtga tcctcccacc tcaaccacct gagtagctgt gccaccacac ccggctaatt
     9361 tttgtatttt ttgtagagat ggagtttcac cttgttgccc aggctgatca caaactcctg
     9421 ggctcaagca atctccccac ttaggcctcc caaagtgcag ggattatagg catgagccac
     9481 cgcaccccgc agtactttcc ctcgtataca agctactctc ctgaaaaaaa tagcaggaac
     9541 ccattccaat gtccaagctc ccctaaagtg ccaactcaga ataactttca tgtttaaaaa
     9601 cagctgtctt cacattgccc attgtgaggt tgtaaaagga agttaagatg tcaagcaatt
     9661 tagattctgt tacccatttc cctatgaaaa gagatgtgtt aaaagtcttt aactacttaa
     9721 agggtcatca tgaaaaataa gaattctatt ccagaagaca gagttataca cagggaggtg
     9781 tattatatac ttgctaataa atttttgtgt aggctcttta gtaaatttat ctgataatgg
     9841 aataatcagc ctttttggtg gaaaggtaca tacagaagct aaatcatcat ctgttaatag
     9901 tgtttaagag atttcttcat tgagatggaa tatgggcaag atgaccttta aggtcttttt
     9961 cacttttatc attggaaatc cccagaagca atctgatagt cttaaaactt tgtcctttct
    10021 aagtcctttg tattcatttt agagctcttc aggaaactag agaattttgg actttggttg
    10081 acaaaagcgg tctgacctgt gttcgtagct gggaacatga aacacaaaat tctgggttta
    10141 atcttctgaa gaaatctata agtagttact ggcataaatg gtattactat actaacaaat
    10201 ttaaaagcct ataatgtatt aaaactcttt tttactcgat tacttttatt tttttaagac
    10261 acagtctcgc ttattgcccc ggctggagtg cagtggcacg gtctcagctc actgcaatct
    10321 ccgcctccca ggttcaagcg attctcctgc ctcagcctcc caagtagctg gaattaatta
    10381 cagacgccca ccaccatgcc cggctaattt ttgtattttt agtagagatg gggtttcacc
    10441 gtgttgccta ggctggtctt gaactcctga cctcaagtga tcaacccacc ttggcctccc
    10501 aaagtgctgg gattacaggc atgagccact gtgcccagcc ctcttgataa tttttattct
    10561 tgtttcttct tatctgtggg actccaatta gaatggggtt gagattgtgg cattcagttt
    10621 tataccaata ttttacccaa gtgtgtcatg gcctttttta atcattttaa tatccccaga
    10681 aatgctagaa gtgtaaagga ataacagagg taaatttgct gtcattttat gccacatcct
    10741 cttttcttac tttgcttctt gatacagatt atagaggtaa ttgtgaagac agttgaacct
    10801 tagaagattg agctaagatt ctttttttgg caacctgcaa gtaatgtttc atttttctat
    10861 tacgtgatct ctttgcccat acagttggca gatgtttttt cccccttttt ctaccttaaa
    10921 cttttttgtt tggagtacag tctaaaccaa catcagttag ccttgatttt ctttcttttt
    10981 tggacaattg tctggttcat tgacaaaagt aacaagtttg gaactaagag atatgttaca
    11041 agctgcactt aacaccacct gaaaacccaa gaatcatctg aaggttttgt cagtagtaag
    11101 gtaggttttg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg aagatggaat
    11161 agggtgaagg tgaagaaaca gcttgacttg tgcctgtttt cagataaagg agtaaagtga
    11221 tctacaaact gtaactatct tgaaagagaa attgatcatc agatggtcga gtactcttgg
    11281 aatgtctctt tgctgtttac tgtggcctct ttacaatggt gagacttatt tctaccctct
    11341 actttccact attatttttt tcccctattg gataatacta gtctgagcag ccctaatact
    11401 agatgtacct aaacagagat acaagccaag tcattgttca gtgtagtcat ttcttagtat
    11461 ctgtttatat tttgtcacac atcagaatgt atattttata gagcatgtgt ttgtctactt
    11521 gtttgtctac tataatatgt ctttaataaa gctaaaaaca ttctga
//
