LOCUS       NM_015541               5363 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens leucine rich repeats and immunoglobulin like domains 1
            (LRIG1), transcript variant 1, mRNA.
ACCESSION   NM_015541
VERSION     NM_015541.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ta,H.M., Roy,D., Zhang,K., Alban,T., Juric,I., Dong,J.,
            Parthasarathy,P.B., Patnaik,S., Delaney,E., Gilmour,C., Zakeri,A.,
            Shukla,N., Rupani,A., Phoon,Y.P., Liu,C., Avril,S., Gastman,B.,
            Chan,T. and Wang,L.L.
  TITLE     LRIG1 engages ligand VISTA and impairs tumor-specific CD8+ T cell
            responses
  JOURNAL   Sci Immunol 9 (95), eadi7418 (2024)
   PUBMED   38758807
  REMARK    GeneRIF: LRIG1 engages ligand VISTA and impairs tumor-specific
            CD8[+] T cell responses.
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Cheng,S., Li,C., Liu,L., Liu,X., Li,M., Zhuo,J., Wang,J., Zheng,W.
            and Wang,Z.
  TITLE     Dysregulation and antimetastatic function of circLRIG1 modulated by
            miR-214-3p/LRIG1 axis in bladder carcinoma
  JOURNAL   Biol Direct 19 (1), 20 (2024)
   PUBMED   38454507
  REMARK    GeneRIF: Dysregulation and antimetastatic function of circLRIG1
            modulated by miR-214-3p/LRIG1 axis in bladder carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5363)
  AUTHORS   Piracha,Z.Z. and Saeed,U.
  TITLE     Leucine-rich repeats and immunoglobulin-like domains protein 1
            (LRIG1) is downregulated in Invasive ductal carcinoma and potential
            prognostic marker of breast cancer
  JOURNAL   J Cancer Res Ther 19 (7), 1870-1879 (2023)
   PUBMED   38376291
  REMARK    GeneRIF: Leucine-rich repeats and immunoglobulin-like domains
            protein 1 (LRIG1) is downregulated in Invasive ductal carcinoma and
            potential prognostic marker of breast cancer.
REFERENCE   4  (bases 1 to 5363)
  AUTHORS   Umeh-Garcia,M., O'Geen,H., Simion,C., Gephart,M.H., Segal,D.J. and
            Sweeney,C.A.
  TITLE     Aberrant promoter methylation contributes to LRIG1 silencing in
            basal/triple-negative breast cancer
  JOURNAL   Br J Cancer 127 (3), 436-448 (2022)
   PUBMED   35440669
  REMARK    GeneRIF: Aberrant promoter methylation contributes to LRIG1
            silencing in basal/triple-negative breast cancer.
REFERENCE   5  (bases 1 to 5363)
  AUTHORS   Hsu,H.L., Chen,H.K., Tsai,C.H., Liao,P.L., Chan,Y.J., Lee,Y.C.,
            Lee,C.C. and Li,C.H.
  TITLE     Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated
            Signaling through Leucine-Rich Repeats and Immunoglobulin-like
            Domains 1 (LRIG1)-Dependent EGFR Degradation
  JOURNAL   Int J Mol Sci 22 (18), 9988 (2021)
   PUBMED   34576152
  REMARK    GeneRIF: Aryl Hydrocarbon Receptor Defect Attenuates
            Mitogen-Activated Signaling through Leucine-Rich Repeats and
            Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5363)
  AUTHORS   Nilsson,J., Starefeldt,A., Henriksson,R. and Hedman,H.
  TITLE     LRIG1 protein in human cells and tissues
  JOURNAL   Cell Tissue Res 312 (1), 65-71 (2003)
   PUBMED   12684867
  REMARK    GeneRIF: Results demonstrate that LRIG1 is an integral cell-surface
            membrane protein that is expressed by specific cells in various
            human tissues and that its 143-kDa form might be cleaved into
            111-kDa and 32-kDa fragments.
REFERENCE   7  (bases 1 to 5363)
  AUTHORS   Suzuki,Y., Miura,H., Tanemura,A., Kobayashi,K., Kondoh,G., Sano,S.,
            Ozawa,K., Inui,S., Nakata,A., Takagi,T., Tohyama,M., Yoshikawa,K.
            and Itami,S.
  TITLE     Targeted disruption of LIG-1 gene results in psoriasiform epidermal
            hyperplasia
  JOURNAL   FEBS Lett 521 (1-3), 67-71 (2002)
   PUBMED   12067728
REFERENCE   8  (bases 1 to 5363)
  AUTHORS   Hedman,H., Nilsson,J., Guo,D. and Henriksson,R.
  TITLE     Is LRIG1 a tumour suppressor gene at chromosome 3p14.3?
  JOURNAL   Acta Oncol 41 (4), 352-354 (2002)
   PUBMED   12234026
  REMARK    GeneRIF: Down-regulation of LIG-1 is associated with cancer
REFERENCE   9  (bases 1 to 5363)
  AUTHORS   Nilsson,J., Vallbo,C., Guo,D., Golovleva,I., Hallberg,B.,
            Henriksson,R. and Hedman,H.
  TITLE     Cloning, characterization, and expression of human LIG1
  JOURNAL   Biochem Biophys Res Commun 284 (5), 1155-1161 (2001)
   PUBMED   11414704
REFERENCE   10 (bases 1 to 5363)
  AUTHORS   Suzuki,Y., Sato,N., Tohyama,M., Wanaka,A. and Takagi,T.
  TITLE     cDNA cloning of a novel membrane glycoprotein that is expressed
            specifically in glial cells in the mouse brain. LIG-1, a protein
            with leucine-rich repeats and immunoglobulin-like domains
  JOURNAL   J Biol Chem 271 (37), 22522-22527 (1996)
   PUBMED   8798419
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099665.2, AB050468.1, AF381545.1 and BC014276.2.
            
            On Jan 7, 2020 this sequence version replaced NM_015541.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB050468.1, AF381545.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000273261.8/ ENSP00000273261.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-601               AC099665.2         103625-104225       c
            602-2083            AB050468.1         506-1987
            2084-2404           AF381545.1         1483-1803
            2405-2686           AB050468.1         2309-2590
            2687-5363           BC014276.2         45-2721
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.1"
     gene            1..5363
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /note="leucine rich repeats and immunoglobulin like
                     domains 1"
                     /db_xref="GeneID:26018"
                     /db_xref="HGNC:HGNC:17360"
                     /db_xref="MIM:608868"
     exon            1..833
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             616..3897
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ortholog of mouse integral membrane
                     glycoprotein LIG-1; leucine-rich repeats and
                     immunoglobulin-like domains protein 1; leucine-rich repeat
                     protein LRIG1"
                     /codon_start=1
                     /product="leucine-rich repeats and immunoglobulin-like
                     domains protein 1 isoform 1 precursor"
                     /protein_id="NP_056356.2"
                     /db_xref="CCDS:CCDS33783.1"
                     /db_xref="GeneID:26018"
                     /db_xref="HGNC:HGNC:17360"
                     /db_xref="MIM:608868"
                     /translation="MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAA
                     CTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNN
                     ELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFP
                     HGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDL
                     NRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNS
                     GSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLS
                     VLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGN
                     KIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKW
                     LPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMV
                     GKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQ
                     VTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATG
                     HPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSIS
                     ANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHH
                     LTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTI
                     AVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETV
                     VRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKA
                     EGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQ
                     EHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYH
                     PDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLL
                     LAPKS"
     exon            834..905
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            906..980
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1118
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1262
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1406
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1550
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1694
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1775
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1847
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..1919
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2083
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2404
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2686
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..3106
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3232
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3233..3385
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..3670
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3671..5363
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5339..5344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /note="hexamer: AATAAA"
     polyA_site      5363
                     /gene="LRIG1"
                     /gene_synonym="LIG-1; LIG1"
                     /note="major polyA site"
ORIGIN      
        1 agcaagcggc tgattgttcg gctgcgaggt cggcgggact cttgctgcgc ggaggagccg
       61 tgcggaaccc gaggctgcgc gcgcgcgcgc gctctctggc cgcggcgtgg ggacagcgag
      121 gcgcactggg gcctcccagc gcggggccgg ccgcgccgtc cagcccgagg tctacggctt
      181 tgcgctccga gcccagagga agatgcctgc cggcaccgag ctcgggctgc ggggctgaac
      241 gcctgtcttc caggcgcagc ggcagcactg ccctcggccg gtgtcggtag cggcactcgg
      301 cgtgccccgg gcggacgaag agcgcaggct gggtacacct tgcccgaatc ggcggagttc
      361 gcagctagcg cgggcgggcc ggccggcccg gatgggcgcg gggtttgcgg cccccgccgg
      421 gtgctccgga gcggcccggg caccgggggc acgctgagtg ccggagccgc ggccgcagag
      481 agaacttggg gcgggggcca tgccccggtg cggagtctag agccgagcgg agcgccccgc
      541 gggcccgccc gcgtctgcgg ccgttcgggt cctcacagtc cccgcgcccg gagttcgcag
      601 cgcgctccag acaagatggc gcggccggtc cggggagggc tcggggcccc gcgccgctcg
      661 ccttgccttc tccttctctg gctgcttttg cttcggctgg agccggtgac cgccgcggcc
      721 ggcccgcggg cgccctgcgc ggccgcctgc acttgcgctg gggactcgct ggactgcggt
      781 gggcgcgggc tggctgcgtt gcccggggac ctgccctcct ggacgcggag cctaaacctg
      841 agttacaaca aactctctga gattgaccct gctggttttg aggacttgcc gaacctacag
      901 gaagtgtacc tcaataataa tgagttgaca gcggtaccat ccctgggcgc tgcttcatca
      961 catgtcgtct ctctctttct gcagcacaac aagattcgca gcgtggaggg gagccagctg
     1021 aaggcctacc tttccttaga agtgttagat ctgagtttga acaacatcac ggaagtgcgg
     1081 aacacctgct ttccacacgg accgcctata aaggagctca acctggcagg caatcggatt
     1141 ggcaccctgg agttgggagc atttgatggt ctgtcacggt cgctgctaac tcttcgcctg
     1201 agcaaaaaca ggatcaccca gcttcctgta agagcattca agctacccag gctgacacaa
     1261 ctggacctca atcggaacag gattcggctg atagagggcc tcaccttcca ggggctcaac
     1321 agcttggagg tgctgaagct tcagcgaaac aacatcagca aactgacaga tggggccttc
     1381 tggggactgt ccaagatgca tgtgctgcac ctggagtaca acagcctggt agaagtgaac
     1441 agcggctcgc tctacggcct cacggccctg catcagctcc acctcagcaa caattccatc
     1501 gctcgcattc accgcaaggg ctggagcttc tgccagaagc tgcatgagtt ggtcctgtcc
     1561 ttcaacaacc tgacacggct ggacgaggag agcctggccg agctgagcag cctgagtgtc
     1621 ctgcgtctca gccacaattc catcagccac attgcggagg gtgccttcaa gggactcagg
     1681 agcctgcgag tcttggatct ggaccataac gagatttcgg gcacaataga ggacacgagc
     1741 ggcgccttct cagggctcga cagcctcagc aagctgactc tgtttggaaa caagatcaag
     1801 tctgtggcta agagagcatt ctcggggctg gaaggcctgg agcacctgaa ccttggaggg
     1861 aatgcgatca gatctgtcca gtttgatgcc tttgtgaaga tgaagaatct taaagagctc
     1921 catatcagca gcgacagctt cctgtgtgac tgccagctga agtggctgcc cccgtggcta
     1981 attggcagga tgctgcaggc ctttgtgaca gccacctgtg cccacccaga atcactgaag
     2041 ggtcagagca ttttctctgt gccaccagag agtttcgtgt gcgatgactt cctgaagcca
     2101 cagatcatca cccagccaga aaccaccatg gctatggtgg gcaaggacat ccggtttaca
     2161 tgctcagcag ccagcagcag cagctccccc atgacctttg cctggaagaa agacaatgaa
     2221 gtcctgacca atgcagacat ggagaacttt gtccacgtcc acgcgcagga cggggaagtg
     2281 atggagtaca ccaccatcct gcacctccgt caggtcactt tcgggcacga gggccgctac
     2341 caatgtgtca tcaccaacca ctttggctcc acctattcac ataaggccag gctcaccgtg
     2401 aatgtgttgc catcattcac caaaacgccc cacgacataa ccatccggac caccaccatg
     2461 gcccgcctcg aatgtgctgc cacaggtcac ccaaaccctc agattgcctg gcagaaggat
     2521 ggaggcacgg atttccccgc tgcccgtgag cgacgcatgc atgtcatgcc ggatgacgac
     2581 gtgtttttca tcactgatgt gaaaatagat gacgcagggg tttacagctg tactgctcag
     2641 aactcagccg gttctatttc agctaatgcc accctgactg tcctagagac cccatccttg
     2701 gtggtcccct tggaagaccg tgtggtatct gtgggagaaa cagtggccct ccaatgcaaa
     2761 gccacgggga accctccgcc ccgcatcacc tggttcaagg gggaccgccc gctgagcctc
     2821 actgagcggc accacttgac ccctgacaac cagctcctgg tggttcagaa cgtggtggca
     2881 gaggatgcgg gccgatatac ctgtgagatg tccaacaccc tgggcacgga gcgagctcac
     2941 agccagctga gcgtcctgcc cgcagcaggc tgcaggaagg atgggaccac ggtaggcatc
     3001 ttcaccattg ctgtcgtgag cagcatcgtc ctgacgtcac tggtctgggt gtgcatcatc
     3061 taccagacca ggaagaagag tgaagagtac agtgtcacca acacagatga aaccgtcgtg
     3121 ccaccagatg ttccaagcta cctctcttct caggggaccc tttctgaccg acaagaaacc
     3181 gtggtcagga ccgagggtgg ccctcaggcc aatgggcaca ttgagagcaa tggtgtgtgt
     3241 ccaagagatg caagccactt tccagagccc gacactcaca gcgttgcctg caggcagcca
     3301 aagctctgtg ctgggtctgc gtatcacaaa gagccgtgga aagcgatgga gaaagctgaa
     3361 gggacacctg ggccacataa gatggaacac ggtggccggg tcgtatgcag tgactgcaac
     3421 accgaagtgg actgttactc caggggacaa gccttccacc cccagcctgt gtccagagac
     3481 agcgcacagc caagtgcgcc aaatggcccg gagccgggtg ggagtgacca agagcattct
     3541 ccacatcacc agtgcagcag gactgccgct gggtcctgcc ccgagtgcca agggtcgctc
     3601 taccccagta accacgatag aatgctgacg gctgtgaaga aaaagccaat ggcatctcta
     3661 gatgggaaag gggattcttc ctggacttta gcaaggttgt atcacccgga ctccacagag
     3721 ctacagcctg catcttcatt aacttcaggc agtccagagc gcgcggaagc ccagtacttg
     3781 cttgtttcca atggccacct ccccaaagca tgtgacgcca gtcccgagtc cacgccactg
     3841 acaggacagc tccccgggaa acagagggtg ccactgctgt tggcaccaaa aagctaggtt
     3901 ttgtctacct cagttcttgt cataccaatc tctacgggaa agagaggtag gagaggctgc
     3961 gaggaagctt gggttcaagc gtcactcatc tgtacatagt tgtaactccc atgtggagta
     4021 tcagtcgctc acaggacttg gatctgaagc acagtaaacg caagagggga tttgtgtaca
     4081 aaaggcaaaa aaagtatttg atatcattgt acataagagt tttcagagat ttcatatata
     4141 tcttttacag aggctatttt aatctttagt gcatggttaa cagaaaaaaa ttatacaatt
     4201 ttgacaatat tatttttcgt atcaggttgc tgtttaattt tggagggggt ggggaaatag
     4261 ttctggtgcc ttaacgcatg gctggaattt atagaggcta caaccacatt tgttcacagg
     4321 agtttttggt gcggggtggg aaggatggaa ggccttggat ttatattgca cttcatagac
     4381 ccctaggctg ctgtgcggtg ggactccaca tgcgccggaa ggagcttcag gtgagcactg
     4441 ctcatgtgtg gatgcccctg caacaggctt ccctgtctgt agagccaggg gtgcaagtgc
     4501 catccacact tgcagtgaat ggcttttcct tttaggttta agtcctgtct gtctgtaagg
     4561 cgtagaatct gtccgtctgt aaggcgtaga atgagggttg ttaatccatc acaagcaaaa
     4621 ggtcagaaca gttaaacact gcctttcctc ctcctcttat tttatgataa aagcaaatgt
     4681 ggccttctca gtatcattcg attgctattt gagactttta aattaaggta aaggctgctg
     4741 gtgttggtac ctgtggattt ttctatactg atgttttcgt tttgccaata taatgagtat
     4801 tacattggcc ttgggggaca gaaaggagga agttctgact tttcagggct accttatttc
     4861 tactaaggac ccagagcagg cctgtccatg ccattccttc gcacagatga aactgagctg
     4921 ggactggaaa ggacagccct tgacctgggt tctgggtata atttgcactt ttgagactgg
     4981 tagctaacca tcttatgagt gccaatgtgt catttagtaa aacttaaata gaaacaaggt
     5041 ccttcaaatg ttcctttggc caaaagctga agggagttac tgagaaaata gttaacaatt
     5101 actgtcaggt gtcatcactg ttcaaaaggt aagcacattt agaattttgt tcttgacagt
     5161 taactgacta atcttacttc cacaaaatat gtgaatttgc tgcttctgag aggcaatgtg
     5221 aaagagggag tattactttt atgtacaaag ttatttattt atagaaattt tggtacagtg
     5281 tacattgaaa accatgtaaa atattgaagt gtctaacaaa tggcattgaa gtgtctttaa
     5341 taaaggttca tttataaatg tca
//
