LOCUS       NM_002319               3177 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens leucine rich repeats and calponin homology domain
            containing 4 (LRCH4), transcript variant 1, mRNA.
ACCESSION   NM_002319
VERSION     NM_002319.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Muller,P.M., Rademacher,J., Bagshaw,R.D., Wortmann,C., Barth,C.,
            van Unen,J., Alp,K.M., Giudice,G., Eccles,R.L., Heinrich,L.E.,
            Pascual-Vargas,P., Sanchez-Castro,M., Brandenburg,L., Mbamalu,G.,
            Tucholska,M., Spatt,L., Czajkowski,M.T., Welke,R.W., Zhang,S.,
            Nguyen,V., Rrustemi,T., Trnka,P., Freitag,K., Larsen,B., Popp,O.,
            Mertins,P., Gingras,A.C., Roth,F.P., Colwill,K., Bakal,C.,
            Pertz,O., Pawson,T., Petsalaki,E. and Rocks,O.
  TITLE     Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals
            spatially organized RAC1 signalling from integrin adhesions
  JOURNAL   Nat Cell Biol 22 (4), 498-511 (2020)
   PUBMED   32203420
REFERENCE   3  (bases 1 to 3177)
  AUTHORS   Aloor,J.J., Azzam,K.M., Guardiola,J.J., Gowdy,K.M.,
            Madenspacher,J.H., Gabor,K.A., Mueller,G.A., Lin,W.C., Lowe,J.M.,
            Gruzdev,A., Henderson,M.W., Draper,D.W., Merrick,B.A. and
            Fessler,M.B.
  TITLE     Leucine-rich repeats and calponin homology containing 4 (Lrch4)
            regulates the innate immune response
  JOURNAL   J Biol Chem 294 (6), 1997-2008 (2019)
   PUBMED   30523158
REFERENCE   4  (bases 1 to 3177)
  AUTHORS   Fejes,A.V., Best,M.G., van der Heijden,W.A., Vancura,A.,
            Verschueren,H., de Mast,Q., Wurdinger,T. and Mannhalter,C.
  TITLE     Impact of Escherichia coli K12 and O18:K1 on human platelets:
            Differential effects on platelet activation, RNAs and proteins
  JOURNAL   Sci Rep 8 (1), 16145 (2018)
   PUBMED   30385858
  REMARK    GeneRIF: Using two bioinformatics pipelines for analysis of RNA
            fingerprints we identified significant effects of E. coli on the
            mRNAs HMBS, ATP2C1 and LRCH4. To see whether these three proteins
            were present in platelets and were influenced by the bacteria, we
            analysed HMBS, ATP2C1 and LRCH4 in platelet lysates and releasates
            by Western blot and ELISA.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3177)
  AUTHORS   Couzens,A.L., Knight,J.D., Kean,M.J., Teo,G., Weiss,A.,
            Dunham,W.H., Lin,Z.Y., Bagshaw,R.D., Sicheri,F., Pawson,T.,
            Wrana,J.L., Choi,H. and Gingras,A.C.
  TITLE     Protein interaction network of the mammalian Hippo pathway reveals
            mechanisms of kinase-phosphatase interactions
  JOURNAL   Sci Signal 6 (302), rs15 (2013)
   PUBMED   24255178
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3177)
  AUTHORS   Foussard,H., Ferrer,P., Valenti,P., Polesello,C., Carreno,S. and
            Payre,F.
  TITLE     LRCH proteins: a novel family of cytoskeletal regulators
  JOURNAL   PLoS One 5 (8), e12257 (2010)
   PUBMED   20805893
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3177)
  AUTHORS   Venkatesan,K., Rual,J.F., Vazquez,A., Stelzl,U., Lemmens,I.,
            Hirozane-Kishikawa,T., Hao,T., Zenkner,M., Xin,X., Goh,K.I.,
            Yildirim,M.A., Simonis,N., Heinzmann,K., Gebreab,F., Sahalie,J.M.,
            Cevik,S., Simon,C., de Smet,A.S., Dann,E., Smolyar,A.,
            Vinayagam,A., Yu,H., Szeto,D., Borick,H., Dricot,A., Klitgord,N.,
            Murray,R.R., Lin,C., Lalowski,M., Timm,J., Rau,K., Boone,C.,
            Braun,P., Cusick,M.E., Roth,F.P., Hill,D.E., Tavernier,J.,
            Wanker,E.E., Barabasi,A.L. and Vidal,M.
  TITLE     An empirical framework for binary interactome mapping
  JOURNAL   Nat Methods 6 (1), 83-90 (2009)
   PUBMED   19060904
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC397219.1, BC018529.1,
            DR422652.1, BC007634.1 and AI491709.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002319.4.
            
            Summary: This gene encodes a protein that contains leucine-rich
            repeats (LRR) at its amino terminus and that is known to be
            involved in ligand binding. The carboxyl terminus may act as a
            membrane anchor. Identified structural elements suggest that the
            encoded protein resembles a receptor. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.920660.1, BC018529.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310300.11/ ENSP00000309689.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                DC397219.1         60-105
            47-1039             BC018529.1         71-1063
            1040-1117           DR422652.1         513-590
            1118-2528           BC018529.1         1142-2552
            2529-3171           BC007634.1         2192-2834
            3172-3177           AI491709.1         20-25               c
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..3177
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /note="leucine rich repeats and calponin homology domain
                     containing 4"
                     /db_xref="GeneID:4034"
                     /db_xref="HGNC:HGNC:6691"
                     /db_xref="MIM:620165"
     exon            1..249
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..2081
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich neuronal protein; leucine rich repeat
                     neuronal 4; leucine-rich repeat and calponin homology
                     domain-containing protein 4; leucine-rich repeats and
                     calponin homology (CH) domain containing 4"
                     /codon_start=1
                     /product="leucine-rich repeat and calponin homology
                     domain-containing protein 4 isoform 1"
                     /protein_id="NP_002310.2"
                     /db_xref="CCDS:CCDS34706.1"
                     /db_xref="GeneID:4034"
                     /db_xref="HGNC:HGNC:6691"
                     /db_xref="MIM:620165"
                     /translation="MAAAVAAPLAAGGEEAAATTSVPGSPGLPGRRSAERALEEAVAT
                     GTLNLSNRRLKHFPRGAARSYDLSDITQADLSRNRFPEVPEAACQLVSLEGLSLYHNC
                     LRCLNPALGNLTALTYLNLSRNQLSLLPPYICQLPLRVLIVSNNKLGALPPDIGTLGS
                     LRQLDVSSNELQSLPSELCGLSSLRDLNVRRNQLSTLPEELGDLPLVRLDFSCNRVSR
                     IPVSFCRLRHLQVILLDSNPLQSPPAQVCLKGKLHIFKYLSTEAGQRGSALGDLAPSR
                     PPSFSPCPAEDLFPGHRYDGGLDSGFHSVDSGSKRWSGNESTDEFSELSFRISELARE
                     PRGPRERKEDGSADGDPVQIDFIDSHVPGEDEERGTVEEQRPPELSPGAGDRERAPSS
                     RREEPAGEERRRPDTLQLWQERERRQQQQSGAWGAPRKDSLLKPGLRAVVGGAAAVST
                     QAMHNGSPKSSASQAGAAAGQGAPAPAPASQEPLPIAGPATAPAPRPLGSIQRPNSFL
                     FRSSSQSGSGPSSPDSVLRPRRYPQVPDEKDLMTQLRQVLESRLQRPLPEDLAEALAS
                     GVILCQLANQLRPRSVPFIHVPSPAVPKLSALKARKNVESFLEACRKMGVPEADLCSP
                     SDLLQGTARGLRTALEAVKRVGGKALPPLWPPSGLGGFVVFYVVLMLLLYVTYTRLLG
                     S"
     exon            250..394
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            395..521
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            522..627
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            628..764
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            765..877
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            878..977
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1068
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1145
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1207
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1324
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1393
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1497
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1581
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1667
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1805
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1883
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..3177
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2509..2514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /note="hexamer: TATAAA"
     polyA_site      2528
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /note="major polyA site"
     regulatory      3134..3139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
                     /note="hexamer: AATAAA"
     polyA_site      3177
                     /gene="LRCH4"
                     /gene_synonym="LRN; LRRN1; LRRN4; PP14183"
ORIGIN      
        1 agtcaggcag cgggagccgc cgggagcgga tggcggcggc cgtagcggct ccactcgccg
       61 ccgggggtga ggaggcggca gccacgacct ccgtgcccgg gtctccaggt ctgccgggga
      121 gacgcagtgc agagcgggcc ctagaggagg ccgtggccac cgggaccctg aacctgtcta
      181 accggcgctt gaagcacttc ccccggggcg cggcccgtag ctacgacctg tcagacatca
      241 cccaggctga cctgtcccgg aaccggtttc ccgaggtgcc cgaggcggcg tgccagctgg
      301 tgtccctgga gggcctgagc ctctaccaca attgcctgag atgcctgaac ccagccttgg
      361 ggaatctcac agccctcacc tacctcaacc tcagccgaaa ccagctgtcg ctgctgccac
      421 cctacatctg ccagctgccc ctgagggtcc tcatcgtcag caacaacaag ctgggagccc
      481 tgccccctga catcggcacc ctgggaagcc tgcgacagct tgacgtgagc agcaacgagc
      541 tccaatccct gccctcggaa ctgtgtggcc tctcttccct gcgggacctc aatgtccgga
      601 ggaaccagct cagtacgctg cccgaagagc tgggggacct ccctctggtc cgcctggatt
      661 tctcctgtaa ccgcgtctcc cgaatcccag tctccttctg ccgcctgagg cacctgcagg
      721 tcattctgct ggacagcaac cctctgcaga gtccacctgc ccaggtctgc ctgaagggga
      781 aacttcacat cttcaagtat ttgtccacag aggccgggca gcgtgggtcg gccctggggg
      841 acctggcccc ttctcggccc ccgagtttca gtccctgccc tgcagaggat ctatttccgg
      901 gacatcggta cgatggtggg ctggactcag gcttccacag cgttgatagt ggcagcaaga
      961 ggtggtctgg aaatgagtca acagatgaat tttcagagct gtcattccgg atctcagagc
     1021 tggcccggga gccccgggga cccagagaac gcaaggagga tggctcagcg gacggagacc
     1081 ctgtgcagat tgacttcatc gacagccatg tccccgggga ggatgaagag cgaggcactg
     1141 tggaggagca gcgaccaccc gaattaagcc ctggggcagg ggacagggag agggcaccaa
     1201 gcagcaggcg ggaggagccg gcaggggagg agcggcggcg cccggacacc ttgcagctgt
     1261 ggcaggagcg ggaacggcgg cagcagcagc agagcggggc gtggggggcc ccgaggaagg
     1321 atagcctctt gaagccaggg ctcagggctg ttgtgggagg ggccgccgcc gtgtccactc
     1381 aagccatgca caacggctcg cctaagtcca gtgcctccca agcaggggct gcagcggggc
     1441 agggagcccc cgcccctgcc cctgcctccc aagagcccct tcccatagct ggaccagcga
     1501 cagcacctgc tccacggcca cttggctcca ttcagagacc aaacagcttc ctcttccgtt
     1561 cctcctctca gagtggctca ggcccttcct caccagactc tgtcctgaga cctcggcggt
     1621 acccccaggt tccagatgag aaggacttaa tgactcagct gcgccaggtc cttgagtccc
     1681 ggctgcagcg gcccctgcct gaggacctgg ccgaggctct ggccagtggg gtcatcctgt
     1741 gccagctggc caaccagcta cggccgcgct ccgtgccctt catccatgtg ccctcccctg
     1801 ctgtgccaaa actcagtgcc ctcaaggctc ggaagaatgt ggagagtttt ctagaagcct
     1861 gtcgaaaaat gggggtgcct gaggctgacc tgtgctcgcc ctcggatctc ctccagggca
     1921 ctgcccgggg gctgcggacc gcgctggagg ccgtgaagcg ggtggggggc aaggccctac
     1981 cgcccctctg gcccccctct ggtctgggcg gcttcgtcgt cttctacgtg gtcctcatgc
     2041 tgctgctcta tgtcacctac actcggctcc tgggttccta ggccccaaaa tcggccctcc
     2101 ctcacccctt tcccttcctc tctatttata aggtccctgc tccacccgac cccacctgcg
     2161 gtgccttcag ccccaaccaa agacactagt gcaccccctt cacagacact gacctcagag
     2221 gccccactct ggtgccccca gaccctgggc ccccagcctc tggcctccct ccagtagccc
     2281 cacgagtccc cacctctcag tgctgacggt gccttcatgt ccccgccggc cctgcccctg
     2341 ccctctgtac cccgtgaggg gtggcaggag ctggagtctc ccccttcctc ctgtgccctc
     2401 cccttccccc cccaacagct gctatggggg ggctaaatta tctctatttt gtagagagga
     2461 tctatatttg taggggttcg gggcccaggc cgggtcccta tctctgtgta taaactgtac
     2521 agaccgtggc cgccctgcct gtgtgtgtgt gtgtgcgcgc gcgcgcgcgt ctgctccgcg
     2581 tgttggtggc tgtggccatg gctctgtgcc caccagcatc tccctcctga gatgccggcc
     2641 tctcatgctc ccggagcgtc cgccaacccc ccgtgtcacc tcccttctgt tatcgctgac
     2701 agctttcttg cgtctcattt gtcgccgagc cccgagcgca cggtgatgct cgggtctgcc
     2761 cccgaccccc tgccacaggc cggaagccgc agggggcacc gtggggaagc taacccggcc
     2821 ccttccccca ggagtcactg tgccagcccc accacatcct ggaagaggag ggggccccgg
     2881 gaaggggcct cccctacatc gctgctgtcg tccacgccct gctggaccgg ccttaggggt
     2941 gaaggtgggg gcacagggcc caccccgccc gggaccccgg gagcagaaga cgcgcctggg
     3001 ctccgcgctc tcagagaagc acgtggtgag ggtggcctgg gcctgggcac ccttggcctg
     3061 ccggcctggc tgcctctggg gtcgagggtc tgggtggaag gaccgcgggt gtcgcaggtg
     3121 tcgtgtcagc cgcaataaac agaagccaga agccctctgg gtggccctca ggtgtaa
//
