LOCUS       NM_020871               4953 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens leucine rich repeats and calponin homology domain
            containing 2 (LRCH2), transcript variant 1, mRNA.
ACCESSION   NM_020871
VERSION     NM_020871.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4953)
  AUTHORS   Muller,P.M., Rademacher,J., Bagshaw,R.D., Wortmann,C., Barth,C.,
            van Unen,J., Alp,K.M., Giudice,G., Eccles,R.L., Heinrich,L.E.,
            Pascual-Vargas,P., Sanchez-Castro,M., Brandenburg,L., Mbamalu,G.,
            Tucholska,M., Spatt,L., Czajkowski,M.T., Welke,R.W., Zhang,S.,
            Nguyen,V., Rrustemi,T., Trnka,P., Freitag,K., Larsen,B., Popp,O.,
            Mertins,P., Gingras,A.C., Roth,F.P., Colwill,K., Bakal,C.,
            Pertz,O., Pawson,T., Petsalaki,E. and Rocks,O.
  TITLE     Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals
            spatially organized RAC1 signalling from integrin adhesions
  JOURNAL   Nat Cell Biol 22 (4), 498-511 (2020)
   PUBMED   32203420
REFERENCE   4  (bases 1 to 4953)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 4953)
  AUTHORS   Couzens,A.L., Knight,J.D., Kean,M.J., Teo,G., Weiss,A.,
            Dunham,W.H., Lin,Z.Y., Bagshaw,R.D., Sicheri,F., Pawson,T.,
            Wrana,J.L., Choi,H. and Gingras,A.C.
  TITLE     Protein interaction network of the mammalian Hippo pathway reveals
            mechanisms of kinase-phosphatase interactions
  JOURNAL   Sci Signal 6 (302), rs15 (2013)
   PUBMED   24255178
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4953)
  AUTHORS   Ng,A. and Xavier,R.J.
  TITLE     Leucine-rich repeat (LRR) proteins: integrators of pattern
            recognition and signaling in immunity
  JOURNAL   Autophagy 7 (9), 1082-1084 (2011)
   PUBMED   21606681
REFERENCE   7  (bases 1 to 4953)
  AUTHORS   Ng,A.C., Eisenberg,J.M., Heath,R.J., Huett,A., Robinson,C.M.,
            Nau,G.J. and Xavier,R.J.
  TITLE     Human leucine-rich repeat proteins: a genome-wide bioinformatic
            categorization and functional analysis in innate immunity
  JOURNAL   Proc Natl Acad Sci U S A 108 Suppl 1 (Suppl 1), 4631-4638 (2011)
   PUBMED   20616063
REFERENCE   8  (bases 1 to 4953)
  AUTHORS   Foussard,H., Ferrer,P., Valenti,P., Polesello,C., Carreno,S. and
            Payre,F.
  TITLE     LRCH proteins: a novel family of cytoskeletal regulators
  JOURNAL   PLoS One 5 (8), e12257 (2010)
   PUBMED   20805893
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC239923.3, BX647193.1,
            AB040928.1 and BG541540.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020871.3.
            
            Summary: This gene encodes a member of the leucine-rich repeat and
            calponin homology domain-containing protein family. These family
            members contain multiple N-terminal leucine-rich repeats, in
            addition to a C-terminal calponin homology domain, a type of domain
            that mediates interactions with actin filaments. These proteins are
            conserved across animal species, and studies of a similar
            Drosophila protein indicate a function as a cytoskeletal
            scaffolding protein. Alternative splicing of this gene results in
            multiple transcript variants. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647193.1, SRR1660807.21254.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317135.13/ ENSP00000325091.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC239923.3         34874-34897         c
            25-986              BX647193.1         1-962
            987-4019            AB040928.1         144-3176
            4020-4431           BG541540.1         141-552
            4432-4953           BX647193.1         4408-4929
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq23"
     gene            1..4953
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /note="leucine rich repeats and calponin homology domain
                     containing 2"
                     /db_xref="GeneID:57631"
                     /db_xref="HGNC:HGNC:29292"
     exon            1..404
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2353
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich repeat and calponin homology
                     domain-containing protein 2; leucine-rich repeats and
                     calponin homology (CH) domain containing 2"
                     /codon_start=1
                     /product="leucine-rich repeat and calponin homology
                     domain-containing protein 2 isoform 1"
                     /protein_id="NP_065922.3"
                     /db_xref="CCDS:CCDS48155.1"
                     /db_xref="GeneID:57631"
                     /db_xref="HGNC:HGNC:29292"
                     /translation="MAASQGGGGNSGGGGCGGGGSSGGCGTAGGGGGGAGGGGGGGGG
                     TLVVPIPVPTLFGQPFPNGPPWNPGSLQPQHTVRSLDRALEEAGSSGILSLSGRKLRD
                     FPGSGYDLTDTTQADLSRNRFTEIPSDVWLFAPLETLNLYHNCIKTIPEAIKNLQMLT
                     YLNISRNLLSTLPKYLFDLPLKVLVVSNNKLVSIPEEIGKLKDLMELDISCNEIQVLP
                     QQMGKLHSLRELNIRRNNLHVLPDELGDLPLVKLDFSCNKVTEIPVCYRKLHHLQVII
                     LDNNPLQVPPAQICLKGKVHIFKYLNIQACCRMDKKPDSLDLPSLSKRMPSQPLTDSM
                     EDFYPNKNHGPDSGIGSDNGEKRLSTTEPSDDDTVSLHSQVSESNREQTSRNDSHIIG
                     SKTDSQKDQEVYDFVDPNTEDVAVPEQGNAHIGSFVSFFKGKEKCSEKSRKNEELGDE
                     KRLEKEQLLAEEEDDDLKEVTDLRKIAAQLLQQEQKNRILNHSTSVMRNKPKQTVECE
                     KSVSADEVNSPLSPLTWQPLENQKDQIDEQPWPESHPIIWQSEERRRSKQIRKEYFKY
                     KSMRKSSSGNENDEQDSDNANMSTQSPVSSEEYDRTDGFSHSPFGLKPRSAFSRSSRQ
                     EYGAADPGFTMRRKMEHLREEREQIRQLRNNLESRLKVILPDDIGAALMDGVVLCHLA
                     NHIRPRSVASIHVPSPAVPKLSMAKCRRNVENFLDACKKLGVSQERLCLPHHILEERG
                     LVKVGVTVQALLELPTTKASQLSVA"
     exon            405..549
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            550..676
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            677..782
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            783..919
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1053
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1141
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1253
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1351
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1410
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1518
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1584
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1633
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1750
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1795
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1846
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1904
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2017
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2155
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2233
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..4953
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4934..4939
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /note="hexamer: GATAAA"
     polyA_site      4953
                     /gene="LRCH2"
                     /gene_synonym="dA204F4.4"
                     /note="major polyA site"
ORIGIN      
        1 acactcgcgg cacaggctga cagtattgtc gggggctatt ctctctccca ggaacatggc
       61 ggcgagtcag ggaggaggcg gtaacagtgg gggcggcggt tgtggtggag gtggaagtag
      121 cggtggctgt ggcacggctg gagggggagg tggcggggct ggagggggag gcggcggcgg
      181 cggcgggacc ctggtggtcc ccatcccggt accgactctt ttcggtcagc cgttccccaa
      241 cgggccgccg tggaacccgg ggagcctgca gcctcagcac accgtgagga gcctggaccg
      301 ggccctggaa gaggcgggca gctccggcat cctgagcctc agtggtcgga aactccgaga
      361 cttcccgggc agcggctacg acctgacgga caccacccaa gcagatcttt ccagaaatcg
      421 ttttacagaa attccttctg atgtctggtt atttgcaccc cttgaaacat taaatttata
      481 tcataattgc atcaaaacca ttcctgaagc cattaaaaat ctgcagatgt taacatacct
      541 taacattagc cgaaatcttt tatcaacatt gccaaaatac ctatttgatc ttccccttaa
      601 agttttggtc gtcagtaata ataaactggt atccattcca gaagaaattg ggaagttaaa
      661 agatttaatg gaattggata ttagctgcaa tgagattcaa gtccttcccc aacaaatggg
      721 aaaattacat tcacttagag agctaaatat aagaagaaat aatcttcatg ttttgccaga
      781 tgaattagga gaccttccct tagtcaagct ggatttctct tgtaataaag tgaccgaaat
      841 tccagtttgt tacagaaagc tgcatcattt acaagtaata attttggata acaatccatt
      901 gcaagtacca ccagcacaga tatgtttaaa gggtaaggtg cacatattta agtacttaaa
      961 tattcaagca tgttgcagaa tggataagaa accagattcc ctggatcttc catcattgag
     1021 taaacgaatg ccctcacagc ctctcacaga cagtatggaa gatttttatc caaataaaaa
     1081 tcatggccct gactctggaa ttggaagtga taatggagag aaacgattat ccacaacaga
     1141 accatcagat gatgacacag tcagccttca ttctcaagtc tcagaatcaa atagagagca
     1201 gacatcaaga aatgacagtc acataatagg aagtaagact gattctcaga aagaccagga
     1261 ggtatatgat tttgttgacc ccaacacaga agatgtagca gttcctgaac agggaaatgc
     1321 acatattgga tcatttgtat cattctttaa gggaaaagaa aaatgttctg aaaaatctcg
     1381 gaaaaatgaa gaattaggag atgaaaaaag acttgagaaa gaacagttac tggcagagga
     1441 agaggatgat gatttgaagg aagtaactga tttgaggaaa atagctgctc agttattgca
     1501 gcaagaacag aagaacagga ttcttaatca ttcaacttct gtgatgagaa acaagccaaa
     1561 acaaactgtg gaatgtgaaa agagtgtctc agcagatgaa gttaattcac cattatcacc
     1621 cctcacctgg cagcccttag aaaatcagaa ggatcaaata gatgaacaac cgtggccaga
     1681 atctcaccct ataatctggc agagtgaaga aaggaggcgg agcaaacaga ttagaaaaga
     1741 atatttcaag tataaatcaa tgaggaagag ttcaagtggc aatgaaaatg atgagcaaga
     1801 cagtgataat gctaatatgt caacacaatc tccagtatca tctgaggaat atgacagaac
     1861 tgatggtttt tcacacagtc cctttggctt gaagcctaga tcagctttta gccgctcatc
     1921 tcgccaagaa tatggggcag cagatccagg atttacaatg agaagaaaga tggaacattt
     1981 acgggaagag cgagagcaaa tacgacaact tcgcaacaat cttgaatcca ggttaaaagt
     2041 aattttgcct gatgacattg gagctgcact gatggatggg gttgttcttt gccatttagc
     2101 caatcatata aggccacgtt ctgttgctag tattcatgta ccatcaccag cagtgcccaa
     2161 actgagcatg gcaaaatgtc gaagaaatgt agaaaatttt cttgatgctt gtaaaaagtt
     2221 gggtgtctca caggaaagac tttgtttgcc tcatcatatt ttggaagaac gaggacttgt
     2281 gaaagttggt gtcacagttc aggcgctcct tgaattacca acaaccaagg catctcagct
     2341 ttctgtggct taatataaca ttttaaaatt ctaagtgtat agtttcattt atttgaagtg
     2401 atttcaaata tttcaaattc atgaataaga atattccgtc aaaaatagtt tgccttaggt
     2461 cacgccttag gattttaaaa aaattacatt gaaacatcaa acttaaacat tgaattgccc
     2521 gaacttcttt gggggaaaac tggattaact taataagagt atttgataaa ctcaaattct
     2581 ctttttacta agtcagttga taatcttgat tatttttcag aaagcagact gatactaaat
     2641 ttaaaatgta gtttcaattt aataatcaca ttaggtttaa tttttttaca tcatagcata
     2701 ttagtaaaca tctggataaa ggtaattttt tttagcaatt tagagcaatt ctatatttga
     2761 gttatttaat acacttctaa ttctagttta tacacaattt cttcaatctt tggtcttagt
     2821 tttctagaaa aaaggagtag aaaaaacaat tctggcttta tattaaattc ttttagtatt
     2881 ataggttggt gccaaaatat tttcagttgt actgtagatt gtttacatgt gaagtgcctg
     2941 tgtaattgat gactcttaca tagtcttaag catttttgca attttttaaa tgtattaata
     3001 gagtcaatgg aactttaaaa tattttagta gattttgtaa agtcagtaat atgtgaggat
     3061 ttaaatgagc ctcacattgt gacatttgtg gttttttagt tagtccattg cagtttccct
     3121 taaattggtt atttcatgtt gtcaaaaata ccaatgttaa tatgattaat ttatgctatt
     3181 taattttgtg aaacttaatc atcttttaaa atgtattatt tgaagagtat tttatttctg
     3241 tattgaaaat gttacacaaa gcagtaaagt ttttgagaac tagtgaagta ctaattatca
     3301 aggacatttt gataaaactg ccaaagtttg ggtttatatc ttccctttga gcagcattct
     3361 atcagtgctg cctttagcta tactcagaga gcagcagttg aggaataggt ttttactcaa
     3421 aatctggcca tttgtgaatc ctgtaaacct tgtgatcttc aattactttg atgttaaatt
     3481 aaacacagac tcttctataa atattttcat gagatactgt gttacacaaa gtccttgttt
     3541 taacttaggt atgggtaaat ttacattggt ggtattgcag gaatttttca aaaacattac
     3601 tgtaatgaat aactgagttt cctatagaag aacctgaata attacttttg tagtaaccaa
     3661 gcaagcatca tatcttaagt atgtgatcat ttgcacttgt tacctcttaa tatggatgca
     3721 acttattaaa aatgttggcc tgggtatttc aaagggtata tataaaaggt aaaaaactag
     3781 tgtgccaaat ggtataggaa caactgtact ttttttgatc agttggcatg aagccataga
     3841 attaaatttt ggctttagaa tacagatgtg ttcattacat tccccaaggg tcaaattgga
     3901 gcttttaaag aattaaagct tctatgagta tatggtttaa gaattaaatt attagtaatt
     3961 tattatttta aatatttgac ctcattagtt gttaattttt tagttggata aaaaaaaaac
     4021 tcgctgacat aatctcccta gtgaaaactt gggtaatata tgtaagaaat ggcaaataaa
     4081 taccttagtt aatgactttt tatgtagaaa tttgacagca ttacaaatta agtgaccatt
     4141 aaaaacacag caatcattca tactgctttc ataggtgtta tgatagtctc tgattaatca
     4201 atctagtatg aattatttta caagtgttgt cctgtttcat atctttgaag tagtagtgaa
     4261 attgtaggcc attttcttat aacatttcag ttacttttca gttaattatt cctttaaaaa
     4321 gaaaatacag cagaacctca ctgtagcatt ctttactgca gagcaaaatt caatttaaga
     4381 tgggcttctc tgaggtccac atcatggcag ttcatttact cttgtgccaa atggcaacac
     4441 cacgtctaaa gcagtttcac agcatggtcc cctacaatgt tataccaagg ctctgatgta
     4501 tttcttttag tagtttctaa gtgtaaaatt tattctaaat gtttttatgg ctattatcta
     4561 tttcatcagt tattgaatta agaatgattt tcattacttg agtgggattt gtgatttaat
     4621 gacagttgaa taatcaaaaa tattttatta ggcccccaaa atttaataga tatctatttc
     4681 tgttacattt tattactgta tggcacaaga aagaagtgac attgggtatg ttagaaatga
     4741 aataaggagt aaaaatgcat taccatttgt ttaataatta tctttacaga atgctagttt
     4801 aatgactttt ttaactgaat tttttgaata aatcagaaat gtttgtattg gtcacagatc
     4861 tatcataatt gcttttctga tgcatttaaa aataaatgga acacttaaat gtatattacc
     4921 gatgcaaact gtggataaag ttgaaagaaa aaa
//
