LOCUS       NM_001164211            5569 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens leucine rich repeats and calponin homology domain
            containing 1 (LRCH1), transcript variant 1, mRNA.
ACCESSION   NM_001164211
VERSION     NM_001164211.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5569)
  AUTHORS   Liu,C., Xu,X., Han,L., Wan,X., Zheng,L., Li,C., Liao,Z., Xiao,J.,
            Zhong,R., Zheng,X., Wang,Q., Li,Z., Chen,H., Wei,B. and Wang,H.
  TITLE     LRCH1 deficiency enhances LAT signalosome formation and CD8+ T cell
            responses against tumors and pathogens
  JOURNAL   Proc Natl Acad Sci U S A 117 (32), 19388-19398 (2020)
   PUBMED   32727906
  REMARK    GeneRIF: LRCH1 deficiency enhances LAT signalosome formation and
            CD8(+) T cell responses against tumors and pathogens.
REFERENCE   4  (bases 1 to 5569)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5569)
  AUTHORS   Wang,Y., Zhang,H., He,H., Ai,K., Yu,W., Xiao,X., Qin,Y., Zhang,L.,
            Xiong,H. and Zhou,G.
  TITLE     LRCH1 suppresses migration of CD4+ T cells and refers to disease
            activity in ulcerative colitis
  JOURNAL   Int J Med Sci 17 (5), 599-608 (2020)
   PUBMED   32210709
  REMARK    GeneRIF: LRCH1 suppresses migration of CD4(+) T cells and refers to
            disease activity in ulcerative colitis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5569)
  AUTHORS   Zintzaras,E., Kitsios,G.D., Ziogas,D.C., Rodopoulou,P. and
            Karachalios,T.
  TITLE     Field synopsis and synthesis of genetic association studies in
            osteoarthritis: the CUMAGAS-OSTEO information system
  JOURNAL   Am J Epidemiol 171 (8), 851-858 (2010)
   PUBMED   20237151
  REMARK    GeneRIF: Meta-analysis and HuGE review of gene-disease association.
            (HuGE Navigator)
            Review article
REFERENCE   7  (bases 1 to 5569)
  AUTHORS   Jiang,Q., Shi,D., Nakajima,M., Dai,J., Wei,J., Malizos,K.N.,
            Qin,J., Miyamoto,Y., Kamatani,N., Liu,B., Tsezou,A., Nakamura,T.
            and Ikegawa,S.
  TITLE     Lack of association of single nucleotide polymorphism in LRCH1 with
            knee osteoarthritis susceptibility
  JOURNAL   J Hum Genet 53 (1), 42 (2008)
   PUBMED   18049793
  REMARK    GeneRIF: results suggest that there is no association between LRCH1
            and knee osteoarthritis susceptibility
REFERENCE   8  (bases 1 to 5569)
  AUTHORS   Ikegawa,S.
  TITLE     New gene associations in osteoarthritis: what do they provide, and
            where are we going?
  JOURNAL   Curr Opin Rheumatol 19 (5), 429-434 (2007)
   PUBMED   17762607
  REMARK    Review article
REFERENCE   9  (bases 1 to 5569)
  AUTHORS   Snelling,S., Sinsheimer,J.S., Carr,A. and Loughlin,J.
  TITLE     Genetic association analysis of LRCH1 as an osteoarthritis
            susceptibility locus
  JOURNAL   Rheumatology (Oxford) 46 (2), 250-252 (2007)
   PUBMED   16891653
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 5569)
  AUTHORS   Spector,T.D., Reneland,R.H., Mah,S., Valdes,A.M., Hart,D.J.,
            Kammerer,S., Langdown,M., Hoyal,C.R., Atienza,J., Doherty,M.,
            Rahman,P., Nelson,M.R. and Braun,A.
  TITLE     Association between a variation in LRCH1 and knee osteoarthritis: a
            genome-wide single-nucleotide polymorphism association study using
            DNA pooling
  JOURNAL   Arthritis Rheum 54 (2), 524-532 (2006)
   PUBMED   16447229
  REMARK    GeneRIF: A genetic variant in LRCH1 was consistently associated
            with knee osteoarthritis in 3 samples from 2 populations.
            GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL138704.12, AL359880.8 and
            AL136958.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001164211.1.
            
            Summary: This gene encodes a protein with a leucine-rich repeat and
            a calponin homology domain. Polymorphism in this gene may be
            associated with susceptibililty to knee osteoarthritis. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.197518.1,
                                           SRR1803614.131794.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMN03465402
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389797.8/ ENSP00000374447.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-534               AL138704.12        4929-5462
            535-679             AL138704.12        101960-102104
            680-806             AL138704.12        120789-120915
            807-912             AL138704.12        133500-133605
            913-1049            AL138704.12        137664-137800
            1050-1177           AL138704.12        139611-139738
            1178-1241           AL138704.12        140892-140955
            1242-1347           AL138704.12        144295-144400
            1348-1472           AL138704.12        146652-146776
            1473-1540           AL138704.12        151095-151162
            1541-1627           AL138704.12        152880-152966
            1628-1717           AL138704.12        156827-156916
            1718-1754           AL138704.12        157027-157063
            1755-1808           AL138704.12        163550-163603
            1809-1881           AL138704.12        164284-164356
            1882-1986           AL359880.8         2299-2403
            1987-2096           AL359880.8         9960-10069
            2097-2234           AL359880.8         15586-15723
            2235-2312           AL359880.8         20660-20737
            2313-5197           AL359880.8         28381-31265
            5198-5569           AL136958.9         101-472
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.13-q14.2"
     gene            1..5569
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="leucine rich repeats and calponin homology domain
                     containing 1"
                     /db_xref="GeneID:23143"
                     /db_xref="HGNC:HGNC:20309"
                     /db_xref="MIM:610368"
     exon            1..534
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..2519
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich repeat and calponin homology
                     domain-containing protein 1; neuronal protein 81;
                     leucine-rich repeats and calponin homology (CH) domain
                     containing 1"
                     /codon_start=1
                     /product="leucine-rich repeat and calponin homology
                     domain-containing protein 1 isoform 1"
                     /protein_id="NP_001157683.2"
                     /db_xref="CCDS:CCDS53865.1"
                     /db_xref="GeneID:23143"
                     /db_xref="HGNC:HGNC:20309"
                     /db_xref="MIM:610368"
                     /translation="MATPGSEPQPFVPALSVATLHPLHHPHHHHHHHQHHGGTGAPGG
                     AGGGGGGSGGFNLPLNRGLERALEEAANSGGLNLSARKLKEFPRTAAPGHDLSDTVQA
                     DLSKNRLVEVPMELCHFVSLEILNLYHNCIRVIPEAIVNLQMLTYLNLSRNQLSALPA
                     CLCGLPLKVLIASNNKLGSLPEEIGQLKQLMELDVSCNEITALPQQIGQLKSLRELNV
                     RRNYLKVLPQELVDLSLVKFDFSCNKVLVIPICFREMKQLQVLLLENNPLQSPPAQIC
                     TKGKVHIFKYLSIQACQIKTADSLYLHTMERPHLHQHVEDGKKDSDSGVGSDNGDKRL
                     SATEPSDEDTVSLNVPMSNIMEEEQIIKEDSCHRLSPVKGEFHQEFQPEPSLLGDSTN
                     SGEERDQFTDRADGLHSEFMNYKARAEDCEELLRIEEDVHWQTEGIISSSKDQDMDIA
                     MIEQLREAVDLLQDPNGLSTDITERSVLNLYPMGSAEALELQDSALNGQIQLETSPVC
                     EVQSDLTLQSNGSQYSPNEIRENSPAVSPTTNSTAPFGLKPRSDPALILPPISFNTLT
                     QAQTWDSSSYSVPSEGDSDNVFLRPQRNLESIDPQFTIRRKMEQMREEKELVEQLRES
                     IEMRLKVSLHEDLGAALMDGVVLCHLVNHIRPRSVASIHVPSPAVPKLSMAKCRRNVE
                     NFLEACRKLGVPEADLCSPCDILQLDFRHIRKTVDTLLALGEKAPPPTSALRSRDLIG
                     FCLVHILFIVLVYITYHWNALSA"
     exon            535..679
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            680..806
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            807..912
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1049
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1177
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1241
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1347
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1472
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1540
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1627
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1717
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1754
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1808
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1881
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1986
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2096
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2234
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2312
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..5569
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4192..4197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="hexamer: AATAAA"
     polyA_site      4213
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="major polyA site"
     regulatory      4245..4250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="hexamer: AATAAA"
     polyA_site      4273
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
     regulatory      5266..5271
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="hexamer: AATAAA"
     polyA_site      5293
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
     regulatory      5546..5551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
                     /note="hexamer: AATAAA"
     polyA_site      5569
                     /gene="LRCH1"
                     /gene_synonym="CHDC1; NP81"
ORIGIN      
        1 gcagtcctta gcttcccggg gacaggaaac cttcaagacc gagctgccac ggccgcctcc
       61 ccgcccgccc cccattctac gcgcctgccc acaccctcct cccctccttc cagcgccttt
      121 cggtggagca ctgcggcact cagcccgagc tgccgttttc ccctcgcggg gaacgctgtg
      181 acccccccgc aggagcggcg gggcggggtg ggggggcccg ggagaagatg gcgacgccgg
      241 gaagcgaacc ccaacctttc gtcccggccc tttcggtagc tactctgcac ccacttcatc
      301 atccccacca ccaccaccac caccatcagc accacggagg aaccggcgcc cccggcgggg
      361 cgggtggtgg cggcggtggc agcgggggct tcaacctgcc cttgaaccgg ggtctggagc
      421 gcgcgcttga ggaggcggcc aactccgggg ggctgaacct gagcgccagg aaattgaagg
      481 aatttccccg taccgcagcc cccgggcacg acctctcgga cacggtgcag gcagacttat
      541 ctaaaaacag actggttgaa gttccaatgg aattgtgcca ttttgtatca ctggaaattc
      601 ttaatctgta tcacaactgt atcagagtca ttcctgaggc catcgttaat ctgcagatgc
      661 tgacttacct gaacttgagt cgaaatcagc tgtccgccct gcctgcctgc ctgtgtggtc
      721 tgcctctcaa agtcttaatc gcaagtaaca acaaacttgg atcattacca gaagagatag
      781 gtcagctcaa acagttaatg gagctggatg tcagctgcaa cgagatcaca gcgttgcccc
      841 agcagatagg tcagttgaaa tctctacgag aactgaatgt cagaagaaat taccttaaag
      901 ttttaccaca agaactagta gatctttcct tggtaaagtt tgacttttcc tgcaacaaag
      961 tgctcgtgat tccaatttgt tttagagaga tgaagcagct gcaagtgtta ctacttgaga
     1021 ataaccctct gcagtctcct ccagcacaga tttgcacaaa gggcaaagtt cacatattta
     1081 agtatctgag catacaagca tgccagatta agacagctga ctccctttat ctccacacca
     1141 tggagaggcc acatttacac cagcacgtgg aagatggcaa gaaggattct gattcgggag
     1201 ttggaagtga taatggagat aagcgattat ctgccaccga gccttctgac gaagacactg
     1261 ttagcctcaa tgtgccaatg tcaaacatca tggaagaaga acagatcatc aaggaggact
     1321 cgtgccatcg ccttagcccc gttaaagggg aatttcatca ggaatttcaa ccggagcctt
     1381 cccttttggg tgacagcacc aactcaggag aagaaagaga ccagtttact gatagagcag
     1441 atggtctcca ttcggaattt atgaactata aggcaagggc agaagactgt gaagagctgt
     1501 tacggataga agaggatgtg cactggcaaa ctgagggcat aataagttca tccaaagatc
     1561 aggacatgga tatagcaatg atcgagcagc tgagagaagc agtagatttg ctgcaagatc
     1621 ccaatggatt aagcacagat attacagaga gaagtgtttt aaacctatat cctatgggat
     1681 cagcagaagc cttagaatta caagattctg cactgaatgg tcaaatacag ctggagacat
     1741 ctccggtgtg tgaggtgcaa agtgatctaa cattacagag taacgggagc cagtattctc
     1801 caaatgagat tagagagaac tcccctgcag tctctcctac cacaaacagc acagctccat
     1861 ttggcctgaa gcctcgatca gacccagccc tcattcttcc tcctatctcc ttcaacacac
     1921 ttacacaggc acagacatgg gacagctcta gctacagtgt tccatctgaa ggagacagtg
     1981 acaatgtgtt tctaagacct cagagaaatt tggaatctat agacccgcag tttacaatcc
     2041 ggaggaaaat ggagcagatg agagaagaga aagagctggt ggaacaactt cgtgagagca
     2101 ttgagatgag attgaaggtc agtctacacg aagacctggg ggcagccctc atggatggtg
     2161 tcgtcctctg ccatctggtc aaccacatcc gcccacggtc ggttgcaagc atccatgtcc
     2221 catcaccagc ggttcccaaa cttagcatgg ccaaatgcag aagaaatgtg gaaaactttt
     2281 tggaagcatg ccgaaaatta ggagtaccag aggctgacct ctgctctccg tgtgacatcc
     2341 tgcagttgga ttttcgtcac attcgaaaga ctgttgacac tctgctggca ctcggggaga
     2401 aagccccacc accaacttct gccctccgct ccagggacct tataggcttc tgtcttgtcc
     2461 atattctctt tatagtgctg gtctatatca cttaccactg gaatgctctg tccgcataat
     2521 gtctgcacgt gcatccaaac gctgtgctct gtcgccctca acctttgcag ggtccttcct
     2581 acctttgagc ctttgccttg caaacttcca tccctgtcat gtcttcagtt atctctcgag
     2641 ttttgaagct gaacagtagc aaatcagatt ttccagaagc acaaactttg tagaatacag
     2701 tttagtataa ttcctctcac ttactgaaat acaacgacga cgactgcaaa gtgtatgcac
     2761 accgcatgct tcctcatcca catagtgcca gcagcagtgc cacgcagttc ctcctctccc
     2821 tcccggtgag ctgctgccct gggcagaggg gaggagaatt ccaggacaag agtgtcaagg
     2881 acagggattt agcatatgga agtctttcct ttgggtcagt attgaactag aattctaatt
     2941 cgggactggg caattgagct gtataggggc caccttgcag ggaggacaga aaactaacat
     3001 tttggcccaa cttgatctat acaaaacttt aataatacca ctactgacca agttggacgt
     3061 gtacacgtac tcacactgcc ttgatggcca ttcgattgga ttcctcccaa atttcctaaa
     3121 aagggagccg cgaagggcgc tgggcagtgt ggccgccaac ttccaccccg gcaagcccct
     3181 ctgtcctatg cagaagggcg ctcaagggaa ggaagtgtcg ttgctgttag agcctcacgt
     3241 ggaggagtca cttaaacacc agttttttac tgcttaattc cttgttaggt cttctcttga
     3301 ggctcttaga aaagcgtttt ccagagagat ttctattttt gaacaatgga acggatcact
     3361 gcttttttgc cacatcacat agtaactgcc ggtccagaat gtgacggatt cgactctatt
     3421 cattttcaaa taaagccatg agccgtggaa cattcttggt cctggtgctt gggttatgat
     3481 ggcaggagtc aagaagaaga ttactttcat tctagaagaa tgtagtttct ctaattattt
     3541 gaaatgttca tttagccttt gattttcact gatattaact agcaaactgc tttaagtcag
     3601 ctcaaaggat tatatagtaa ctatatctgc atttggagca atgtgatcag tttgcattta
     3661 aaaggaaaaa aaagaatttt atcttagcca gaatgtccct ggattcaggg gtgtctttgt
     3721 ataatatgag agggccttgt tccaaggtca aggcagcctc cttattttac atgctgtttg
     3781 ccaaatcttg tttcttagct tggggagatg atggacttag cttcctcaag ataaatttct
     3841 agtttattaa gatgcaaaca gctctcatag atggctacta cgaagaaaat cttatttttc
     3901 tgaacatttt catgaatcca ggggacttga aaatatggaa gacccacata gttagaagaa
     3961 tatatttata aagattcctt gctgctaagt cagatcagat ttgctaacag gaagcattct
     4021 ttacatgaca gtatcttgag ttatgtgagt tttttttcct cctgactttg tgttgattgg
     4081 tgaaatgcag ggtatgtgga agttatctaa ttaacctcag ttgtatatga ataacccaca
     4141 gatgtactga attacttttg gtgctatctt gtactcttca atctgtaaca caataaaatc
     4201 cctttgtacg atgtctaatg agcaccctga gccataaatt gcttaataaa cacattttgg
     4261 gtgattattc caagttttta tcagcccatt gatacatgta ttcatgagct ctcagcattc
     4321 ccatccagct ctgcagtgca ttgaggcttc tctttaatgg tcaccactgt ggtggttttt
     4381 cccttctttc tggggagaaa atgggaaaaa aaaaaagaaa acttactggg ttgccacctt
     4441 aaaataatat caataaaaac tgagtaggac actcatgtaa gagtagattt aattttcagt
     4501 gttgatatag ttcaattaaa acatgttaaa gacaaattaa aagacattta tattttaatt
     4561 ctggcatcca aagtattctt tggacgcact ttgatttttt ttgtgtcatt aatttgtctg
     4621 tactctgctg cgctgcactt cttgggattt attggatgat gttttttcat tagtagtatg
     4681 gtaactagca gaacacaatt aatttagtcc tttaggcaaa aatttgaatg aactgttctg
     4741 tccattgcca acccattaat ttctaatcag aatattaagc ttctctgtgg tttttctcca
     4801 aggtacccgt gcaccagccc atatgctaac tggatgcctg cggatgcctg cccttgcagc
     4861 ttgactgggg atacctggct gacctcctta gagtctgaga agtagtcagg gatgtcactg
     4921 agtggtttat tgtgctgacc aaatctcctc ctcacaagaa agaccgtttt ttcagtactc
     4981 ccttccaatg ttagataaaa ggagccaagt atctatttac attttatttt gaaatagcca
     5041 tttgtattct ctttctccag tttctccaac tggtggcaac tctccactca gtgtcaggaa
     5101 ttccaaaatt tgtgacacct aatttctaat catctttcct atttatggat ttctggtttg
     5161 ttatttttag ggagagacac ttatgaaatg gggctggtgg aaaggggccc cgcagaacta
     5221 caatgtatga taatcgagta taaatttcat caatgagagt agataaataa aggcacttga
     5281 tagcctgctg ctatttgttt gtaagaaatt aaaactagcg cgtggtgagc tgggttatct
     5341 ctcgaaaact catgtagatg cctgattgag tcataaggac aaaagggtgt tttgcctttg
     5401 cctgtgggga aaaagtaggg atgatatttt aaaattttaa gaaactgaaa gttgttttgg
     5461 attaggtgaa aaatacttta atatgatttt atttcaggag acttgatttt taaaaattag
     5521 gcttcgtatt tcaaaattag ttctcaataa acaatcaatt tggtatata
//
