LOCUS       NM_004811               1828 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens leupaxin (LPXN), transcript variant 2, mRNA.
ACCESSION   NM_004811
VERSION     NM_004811.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Fang,M., Liu,X., Xu,W., Wang,X., Xu,L., Zhao,T.J., Li,P. and
            Yang,H.
  TITLE     Paxillin family proteins Hic-5 and LPXN promote lipid storage by
            regulating the ubiquitination degradation of CIDEC
  JOURNAL   J Biol Chem 300 (2), 105610 (2024)
   PUBMED   38159847
  REMARK    GeneRIF: Paxillin family proteins Hic-5 and LPXN promote lipid
            storage by regulating the ubiquitination degradation of CIDEC.
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1828)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1828)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1828)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1828)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   7  (bases 1 to 1828)
  AUTHORS   Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
            Stettler-Gill,M. and Peters,E.C.
  TITLE     Robust phosphoproteomic profiling of tyrosine phosphorylation sites
            from human T cells using immobilized metal affinity chromatography
            and tandem mass spectrometry
  JOURNAL   Anal Chem 76 (10), 2763-2772 (2004)
   PUBMED   15144186
REFERENCE   8  (bases 1 to 1828)
  AUTHORS   Gupta,A., Lee,B.S., Khadeer,M.A., Tang,Z., Chellaiah,M.,
            Abu-Amer,Y., Goldknopf,J. and Hruska,K.A.
  TITLE     Leupaxin is a critical adaptor protein in the adhesion zone of the
            osteoclast
  JOURNAL   J Bone Miner Res 18 (4), 669-685 (2003)
   PUBMED   12674328
  REMARK    GeneRIF: leupaxin may be a critical nucleating component of the
            osteoclast podosomal signaling complex
REFERENCE   9  (bases 1 to 1828)
  AUTHORS   Liu,S., Thomas,S.M., Woodside,D.G., Rose,D.M., Kiosses,W.B.,
            Pfaff,M. and Ginsberg,M.H.
  TITLE     Binding of paxillin to alpha4 integrins modifies integrin-dependent
            biological responses
  JOURNAL   Nature 402 (6762), 676-681 (1999)
   PUBMED   10604475
REFERENCE   10 (bases 1 to 1828)
  AUTHORS   Lipsky,B.P., Beals,C.R. and Staunton,D.E.
  TITLE     Leupaxin is a novel LIM domain protein that forms a complex with
            PYK2
  JOURNAL   J Biol Chem 273 (19), 11709-11713 (1998)
   PUBMED   9565592
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF062075.1.
            
            On Feb 27, 2020 this sequence version replaced NM_004811.2.
            
            Summary: The product encoded by this gene is preferentially
            expressed in hematopoietic cells and belongs to the paxillin
            protein family. Similar to other members of this
            focal-adhesion-associated adaptor-protein family, it has four
            leucine-rich LD-motifs in the N-terminus and four LIM domains in
            the C-terminus. It may function in cell type-specific signaling by
            associating with PYK2, a member of focal adhesion kinase family. As
            a substrate for a tyrosine kinase in lymphoid cells, this protein
            may also function in, and be regulated by, tyrosine kinase
            activity. Alternative splicing results in multiple transcript
            variants encoding distinct isoforms.[provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (2) uses an alternate 5' terminal
            exon, which results in a distinct 5' UTR and novel 5' coding region
            compared to variant 1. It encodes isoform 2, which is shorter than
            and has a distinct N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223165.1, AF062075.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395074.7/ ENSP00000378512.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1660              AF062075.1         10-1669
            1661-1828           AF062075.1         1671-1838
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..1828
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /note="leupaxin"
                     /db_xref="GeneID:9404"
                     /db_xref="HGNC:HGNC:14061"
                     /db_xref="MIM:605390"
     exon            1..97
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1245
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="leupaxin isoform 2"
                     /protein_id="NP_004802.1"
                     /db_xref="CCDS:CCDS7969.1"
                     /db_xref="GeneID:9404"
                     /db_xref="HGNC:HGNC:14061"
                     /db_xref="MIM:605390"
                     /translation="MEELDALLEELERSTLQDSDEYSNPAPLPLDQHSRKETNLDETS
                     EILSIQDNTSPLPAQLVYTTNIQELNVYSEAQEPKESPPPSKTSAAAQLDELMAHLTE
                     MQAKVAVRADAGKKHLPDKQDHKASLDSMLGGLEQELQDLGIATVPKGHCASCQKPIA
                     GKVIHALGQSWHPEHFVCTHCKEEIGSSPFFERSGLAYCPNDYHQLFSPRCAYCAAPI
                     LDKVLTAMNQTWHPEHFFCSHCGEVFGAEGFHEKDKKPYCRKDFLAMFSPKCGGCNRP
                     VLENYLSAMDTVWHPECFVCGDCFTSFSTGSFFELDGRPFCELHYHHRRGTLCHGCGQ
                     PITGRCISAMGYKFHPEHFVCAFCLTQLSKGIFREQNDKTYCQPCFNKLFPL"
     exon            98..255
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            256..302
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            303..402
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            403..570
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            571..744
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            745..826
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            827..975
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1828
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1803..1808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /note="hexamer: AATAAA"
     polyA_site      1828
                     /gene="LPXN"
                     /gene_synonym="LDPL"
                     /note="major polyA site"
ORIGIN      
        1 agttcctttg cggtcctctt cttcagcaca tgccaaagct gttcctcacg gcctgtgaga
       61 caagagcatc ttggatgtag gacaatggaa gagttagatg ccttattgga ggaactggaa
      121 cgctccaccc ttcaggacag tgatgaatat tccaacccag ctcctcttcc cctggatcag
      181 cattccagaa aggagactaa ccttgatgag acttcggaga tcctttctat tcaggataac
      241 acaagtccct tgccggcgca gctcgtgtat actaccaata tccaggagct caatgtctac
      301 agtgaagccc aagagccaaa ggaatcacca ccaccttcta aaacgtcagc agctgctcag
      361 ttggatgagc tcatggctca cctgactgag atgcaggcca aggttgcagt gagagcagat
      421 gctggcaaga agcacttacc agacaagcag gatcacaagg cctccctgga ctcaatgctt
      481 gggggtctgg agcaggaatt gcaggacctt ggcattgcca cagtgcccaa gggccattgt
      541 gcatcctgcc agaaaccgat tgctgggaag gtgatccatg ctctagggca atcatggcat
      601 cctgagcatt ttgtctgtac tcattgcaaa gaagagattg gctccagtcc cttctttgag
      661 cggagtggct tggcctactg ccccaacgac taccaccaac ttttttctcc acgctgtgct
      721 tactgcgctg ctcccatcct ggataaagtg ctgacagcaa tgaaccagac ctggcaccca
      781 gagcacttct tctgctctca ctgcggagag gtgtttggtg cagaaggctt tcatgagaag
      841 gacaagaagc catattgccg aaaggatttc ttagccatgt tctcacccaa gtgtggtggc
      901 tgcaatcgcc cagtgttgga aaactacctt tcagccatgg acactgtctg gcacccagag
      961 tgctttgttt gtggggactg cttcaccagt ttttctactg gctccttctt tgaactggat
     1021 ggacgtccat tctgtgagct ccattaccat caccgccggg gaacgctctg ccatgggtgt
     1081 gggcagccca tcactggccg ttgtatcagt gccatggggt acaagttcca tcctgagcac
     1141 tttgtgtgtg ctttctgcct gacacagttg tcgaagggca ttttcaggga gcagaatgac
     1201 aagacctatt gtcaaccttg cttcaataag ctcttcccac tgtaatgcca actgatccat
     1261 agcctcttca gattccttat aaaatttaaa ccaagagagg agaggaaagg gtaaattttc
     1321 tgttactgac cttctgctta atagtcttat agaaaaagga aaggtgatga gcaaataaag
     1381 gaacttctag actttacatg actaggctga taatcttatt ttttaggctt ctatacagtt
     1441 aattctataa attctctttc tccctctctt ctccaatcaa gcacttggag ttagatctag
     1501 gtccttctat ctcgtccctc tacagatgta ttttccactt gcataattca tgccaacact
     1561 ggttttctta ggtttctcca ttttcacctc tagtgatggc cctactcata tcttctctaa
     1621 tttggtcctg atacttgttt cttttcacgt tttcccattt ccctgtggct cactgtctta
     1681 caatcactgc tgtggaatca tgataccact tttagctctt tgcatcttcc ttcagtgtat
     1741 ttttgttttt caagaggaag tagattttaa ctggacaact ttgagtactg acatcattga
     1801 taaataaact ggcttgtggt ttcaataa
//
