LOCUS       NM_001349206            5448 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens lipin 1 (LPIN1), transcript variant 12, mRNA.
ACCESSION   NM_001349206
VERSION     NM_001349206.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Wang,F., Liu,Y., Dong,Y., Zhao,M., Huang,H., Jin,J., Fan,L. and
            Xiang,R.
  TITLE     Haploinsufficiency of Lipin3 leads to hypertriglyceridemia and
            obesity by disrupting the expression and nucleocytoplasmic
            localization of Lipin1
  JOURNAL   Front Med 18 (1), 180-191 (2024)
   PUBMED   37776435
  REMARK    GeneRIF: Haploinsufficiency of Lipin3 leads to hypertriglyceridemia
            and obesity by disrupting the expression and nucleocytoplasmic
            localization of Lipin1.
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Renard,P., Caccavelli,L., Legendre,A., Tuchmann-Durand,C.,
            Balakirouchenane,D., Blanchet,B., Narjoz,C., Straube,M., Hubas,A.,
            Garros,A., Mention,K., Bednarek,N., Goudin,N., Broissand,C.,
            Schlatter,J., Cisternino,S., Cagnard,N., van Endert,P., Diana,J.,
            de Calbiac,H. and de Lonlay,P.
  TITLE     Hydroxychloroquine sulfate: A novel treatment for lipin-1
            deficiency?
  JOURNAL   Biomed Pharmacother 163, 114813 (2023)
   PUBMED   37150031
  REMARK    GeneRIF: Hydroxychloroquine sulfate: A novel treatment for lipin-1
            deficiency?
REFERENCE   3  (bases 1 to 5448)
  AUTHORS   Dai,W., Zheng,P., Luo,D., Xie,Q., Liu,F., Shao,Q., Zhao,N. and
            Qian,K.
  TITLE     LPIN1 Is a Regulatory Factor Associated With Immune Response and
            Inflammation in Sepsis
  JOURNAL   Front Immunol 13, 820164 (2022)
   PUBMED   35222395
  REMARK    GeneRIF: LPIN1 Is a Regulatory Factor Associated With Immune
            Response and Inflammation in Sepsis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5448)
  AUTHORS   Hsu,W.H., Huang,Y.H., Chen,P.R. and Hsieh,L.S.
  TITLE     NLIP and HAD-like Domains of Pah1 and Lipin 1 Phosphatidate
            Phosphatases Are Essential for Their Catalytic Activities
  JOURNAL   Molecules 26 (18), 5470 (2021)
   PUBMED   34576941
  REMARK    GeneRIF: NLIP and HAD-like Domains of Pah1 and Lipin 1
            Phosphatidate Phosphatases Are Essential for Their Catalytic
            Activities.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5448)
  AUTHORS   Chen,Y., Rui,B.B., Tang,L.Y. and Hu,C.M.
  TITLE     Lipin family proteins--key regulators in lipid metabolism
  JOURNAL   Ann Nutr Metab 66 (1), 10-18 (2015)
   PUBMED   25678092
  REMARK    Review article
REFERENCE   6  (bases 1 to 5448)
  AUTHORS   Csaki,L.S., Dwyer,J.R., Fong,L.G., Tontonoz,P., Young,S.G. and
            Reue,K.
  TITLE     Lipins, lipinopathies, and the modulation of cellular lipid storage
            and signaling
  JOURNAL   Prog Lipid Res 52 (3), 305-316 (2013)
   PUBMED   23603613
  REMARK    Review article
REFERENCE   7  (bases 1 to 5448)
  AUTHORS   Han,G.S. and Carman,G.M.
  TITLE     Characterization of the human LPIN1-encoded phosphatidate
            phosphatase isoforms
  JOURNAL   J Biol Chem 285 (19), 14628-14638 (2010)
   PUBMED   20231281
REFERENCE   8  (bases 1 to 5448)
  AUTHORS   Cao,H. and Hegele,R.A.
  TITLE     Identification of single-nucleotide polymorphisms in the human
            LPIN1 gene
  JOURNAL   J Hum Genet 47 (7), 370-372 (2002)
   PUBMED   12111372
  REMARK    GeneRIF: LPIN1 mutations are not commonly seen in patients with
            lipodystrophy who had no mutations in known disease genes.
REFERENCE   9  (bases 1 to 5448)
  AUTHORS   Peterfy,M., Phan,J., Xu,P. and Reue,K.
  TITLE     Lipodystrophy in the fld mouse results from mutation of a new gene
            encoding a nuclear protein, lipin
  JOURNAL   Nat Genet 27 (1), 121-124 (2001)
   PUBMED   11138012
REFERENCE   10 (bases 1 to 5448)
  AUTHORS   Lehner,R. and Kuksis,A.
  TITLE     Biosynthesis of triacylglycerols
  JOURNAL   Prog Lipid Res 35 (2), 169-201 (1996)
   PUBMED   8944226
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012456.10 and AI379040.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001349206.1.
            
            Summary: This gene encodes a magnesium-ion-dependent phosphatidic
            acid phosphohydrolase enzyme that catalyzes the penultimate step in
            triglyceride synthesis including the dephosphorylation of
            phosphatidic acid to yield diacylglycerol. Expression of this gene
            is required for adipocyte differentiation and it also functions as
            a nuclear transcriptional coactivator with some peroxisome
            proliferator-activated receptors to modulate expression of other
            genes involved in lipid metabolism. Mutations in this gene are
            associated with metabolic syndrome, type 2 diabetes, acute
            recurrent rhabdomyolysis, and autosomal recessive acute recurrent
            myoglobinuria (ARARM). This gene is also a candidate for several
            human lipodystrophy syndromes. [provided by RefSeq, Mar 2017].
            
            Transcript Variant: This variant (12) contains an alternate
            in-frame exon in the coding region compared to variant 1. The
            encoded isoform (7; also known as beta) is longer than isoform 1.
            Variants 10-12 encode the same isoform (7).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.206387.1,
                                           SRR1803611.40703.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000674199.1/ ENSP00000501331.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AC012456.10        24097-24136
            41-241              AC012456.10        42998-43198
            242-337             AC012456.10        45228-45323
            338-645             AC012456.10        48837-49144
            646-771             AC012456.10        51085-51210
            772-879             AC012456.10        53551-53658
            880-1006            AC012456.10        56984-57110
            1007-1313           AC012456.10        59666-59972
            1314-1407           AC012456.10        61294-61387
            1408-1598           AC012456.10        62351-62541
            1599-1692           AC012456.10        64539-64632
            1693-1762           AC012456.10        65852-65921
            1763-1855           AC012456.10        69379-69471
            1856-1935           AC012456.10        72873-72952
            1936-2062           AC012456.10        80372-80498
            2063-2211           AC012456.10        81888-82036
            2212-2298           AC012456.10        82535-82621
            2299-2451           AC012456.10        92553-92705
            2452-2566           AC012456.10        96949-97063
            2567-2670           AC012456.10        97876-97979
            2671-5437           AC012456.10        102097-104863
            5438-5448           AI379040.1         6-16                c
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.1"
     gene            1..5448
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /note="lipin 1"
                     /db_xref="GeneID:23175"
                     /db_xref="HGNC:HGNC:13345"
                     /db_xref="MIM:605518"
     exon            1..40
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            41..241
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..2830
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /EC_number="3.1.3.4"
                     /note="isoform 7 is encoded by transcript variant 12;
                     phosphatidate phosphatase LPIN1"
                     /codon_start=1
                     /product="phosphatidate phosphatase LPIN1 isoform 7"
                     /protein_id="NP_001336135.1"
                     /db_xref="CCDS:CCDS92716.1"
                     /db_xref="GeneID:23175"
                     /db_xref="HGNC:HGNC:13345"
                     /db_xref="MIM:605518"
                     /translation="MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQ
                     CSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMH
                     LATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRR
                     KSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLS
                     LAVIYPQSASYPNSDREWSPTPSSLVDCKRTAPHLAVAAEGGLSSSCPPQSSLFHPSE
                     SPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQAAKSSSPHKMKESSPLSS
                     RKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAP
                     LLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYF
                     PKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGL
                     SDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQ
                     KPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQL
                     SLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRLTSEQLKSLKLKNGPND
                     VVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQG
                     IAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSA
                     LHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFT
                     VNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLP
                     PFENQDIHSASA"
     exon            242..337
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            338..645
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            646..771
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            772..879
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1006
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1313
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1407
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1598
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1692
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1762
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1855
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1935
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2062
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2211
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2298
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2451
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2566
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..2670
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..5448
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5415..5420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /note="hexamer: AATAAA"
     polyA_site      5448
                     /gene="LPIN1"
                     /gene_synonym="PAP1"
                     /note="major polyA site"
ORIGIN      
        1 gtgtttgcaa tacaaaggcg gccacgcgcg gcgccgctcg gtgcagacca tgaattacgt
       61 ggggcagtta gccggccagg tgtttgtcac cgtgaaggag ctctacaagg ggctgaatcc
      121 cgccacactc tcagggtgca ttgacatcat tgtcatccgc cagcccaatg gaaacctcca
      181 atgctcccct ttccacgtcc gctttgggaa gatgggggtc ctgcgctccc gagagaaagt
      241 ggttgacata gaaatcaatg gggaatctgt ggatttgcat atgaaattgg gagataatgg
      301 agaagcattt tttgttcaag aaacagataa tgatcaggaa gttatcccta tgcacctggc
      361 cacctccccc atcctgtcag aaggagcttc gagaatggaa tgccagctga aaaggggctc
      421 tgtggacagg atgagaggcc tggaccccag cacgccagcc caagtgatcg ctcccagcga
      481 gacgccgtca agcagctctg tagtaaagaa gagaagaaaa aggaggagaa agtcacagct
      541 ggacagcctg aagagagatg acaacatgaa cacatctgag gatgaggaca tgttccccat
      601 cgagatgagc tcggatgagg ccatggagct gctggagagc agcagaactc ttcctaatga
      661 tatacctcca ttccaagatg atattcctga ggaaaacctc tccctggctg tgatttaccc
      721 tcagtcagcc tcatacccta attcggatag agagtggtca cccactccca gtagcctggt
      781 agattgcaaa aggactgccc ctcatcttgc agttgcggcc gagggaggtc tgtctagttc
      841 ttgccctcca cagtcttccc tgttccatcc ttcggaaagt ccttccggtt cccgaccttc
      901 aacacctaaa agtgattcag aattggtcag caagtccacg gaaaggacag ggcagaagaa
      961 cccagaaatg ctttggctgt ggggagagct gccgcaggct gctaagtctt cttctccaca
     1021 caagatgaaa gagtccagcc cattgagcag tagaaaaatt tgtgataaaa gtcactttca
     1081 ggccattcac agcgaatctt cagacacttt tagtgaccaa tcgccaactc tggtcggtgg
     1141 ggcacttttg gaccagaaca agcctcagac agaaatgcag tttgtgaatg aagaagacct
     1201 ggagacctta ggagcagcag cgccactctt gcccatgatc gaggagctca aacccccctc
     1261 tgccagtgta gtccagacag caaacaagac ggattctcct tccaggaaaa gagataaacg
     1321 aagccgacat cttggtgctg acggcgtcta cttggatgac ctcacagaca tggatcctga
     1381 agtggcggcc ctgtattttc ccaaaaacgg agatccttcc ggactcgcaa aacatgcaag
     1441 cgacaacgga gcccggtcag ccaaccagtc cccgcagtcg gtgggcagct cgggcgtgga
     1501 cagtggcgtg gagagcacct cggacgggct gagggacctc ccttccatcg ccatctccct
     1561 ctgcgggggc ctcagcgacc accgggagat cacgaaagat gcattcctgg agcaagctgt
     1621 gtcatatcaa cagtttgtgg acaaccccgc tattatcgat gaccccaatc tcgtggtaaa
     1681 gattgggagt aaatattata actggacaac agcagcaccc ctcctcctgg caatgcaggc
     1741 cttccagaaa cctttgccaa aggccactgt ggaatctatc atgagggata aaatgcccaa
     1801 aaagggagga agatggtggt tttcatggag gggaagaaac accacaatca aggaggaaag
     1861 taagccagag cagtgcttgg ctggcaaggc ccatagcacc ggagagcaac cgccgcagct
     1921 cagcttggcc accagggtaa agcatgaatc atcctccagt gatgaggagc gcgcagctgc
     1981 caagccatca aacgcaggcc acctccctct tctgcctaat gtcagctaca agaagactct
     2041 ccggctgact tccgagcagc ttaaaagctt gaagttgaag aatggcccca acgacgtggt
     2101 tttcagtgtc accacgcagt accaaggcac gtgccgctgt gagggcacca tctatctgtg
     2161 gaactgggat gataaagtca tcatttctga tattgatggg acaattacca gatcagatac
     2221 tcttggccac attttgccca cccttgggaa ggattggacc catcagggca tcgctaagct
     2281 gtaccataaa gtgagccaga atggatataa atttctctac tgttctgccc gtgccatcgg
     2341 gatggcggac atgacgcggg gctacctgca ctgggtcaac gagaggggca cggtgctgcc
     2401 ccaggggccc ctgctgctga gtcccagcag cctcttctct gccctgcaca gagaagtgat
     2461 tgaaaagaag ccagaaaagt ttaaagtcca gtgtttgaca gacatcaaaa acctgttttt
     2521 ccccaacaca gaaccctttt atgctgcttt tggaaaccga ccagctgatg tgtattcata
     2581 caagcaagta ggagtgtctt tgaatagaat atttaccgtc aaccctaaag gagagctggt
     2641 acaggaacat gcaaagacca acatctcttc gtatgtgaga ctctgtgaag tagtcgacca
     2701 cgttttcccg ttgctgaaaa gaagccattc ttcagacttt ccctgttcgg ataccttcag
     2761 taacttcacc ttttggagag agccactgcc accttttgaa aaccaggaca ttcattctgc
     2821 ctcagcgtaa aatgtcccaa gcagcctctt gccagcagtg cagagcctgg ttgtcaccca
     2881 ttaaaggata ggtctccccg gagtgcacag ctccacctgg gagcctggcg cgtcatcatt
     2941 ggcctgacag cagagagaat tgagaagcat ttctcccctg ccccaccccg gggctgacat
     3001 ttctaagcaa gataggaagg gagcactttc taggctagga gttgggtgca tttgtaccgt
     3061 gaaaagcatt cctcagttgt ggcttaatgc cagttacgac gctgcctttc cggcctgctc
     3121 cagcaagtag ctactggttc acgtgcagtt tggggctgtg aaacctaggc agaaggcggc
     3181 tgtctgaggg ctgtccccgc ctaggacagg gtcaatcgag gaatgccaga tgtgcacggt
     3241 ttttggcaaa gtagggggca catttccatt atagcaatgt tagtgccacc accttctgaa
     3301 cacagtgggg agggctgtga aggctcatgt gacctggatc tgaggtctct gatagaaatc
     3361 tggacgccac cgggtccagg cctggcctca gacttggcct tgtggatggg ccccttacag
     3421 tatttgctga ctagtctcat ttttaggtga taaatttttc tttaattcct ttggttaaag
     3481 atagtctatt tcattggcat atctcccccc agtttttgtg gctcaaggct ggaatattta
     3541 tgccttaata tatctatggc agacatttaa gaatgcgctt tatctagctc atggtaactt
     3601 tgcaacgcct tagattaaaa tgacagtaaa tattactaag gcagtatttt gaatgagttt
     3661 gacactgccg gcttccttcc atccagcgag gtggtgctga cagtgtggac ttgagcacac
     3721 ttatgccaaa tgataatgat actgacttct gttgggagct ctccaaagaa actggttggt
     3781 tttaagaaaa tagtttcaag aagttcaact atattctttt agatattatg tattgtttta
     3841 ctctgattag gttactgtga taggcattta ttcatattct ttctatacca ctgtcattaa
     3901 tatattaaaa agatgtatgt gttagactat cgaaagggcc ttattctctc tttctcatag
     3961 actgaccttc ttttggaatt tctgagtcat ttattttcct tagctttttc cactcaaatt
     4021 aagggcaagc gaaaaagtaa taatttggca ttctttaagc ctacagaatg tgattctttc
     4081 acttgtttat tacactggct cgtggacaga acaatttgaa aagtgaaaga attattttgg
     4141 taaaagattt tgctttactt ttcgaagcat tattttttta aagagtgttt tactccaacg
     4201 attgaaacat tttcctattt aaatttcatt gttagaatca caggaggcaa aaaatggaac
     4261 ggttgaatga aattttactc tttctgtgaa agaaaatcca cagagttgtt gcctccgttg
     4321 tagttggtgg gccccgttag cattggatgc ctttgccaaa tggttcatgt ggacacacaa
     4381 aggcaaacag atctgccatc gatcgcagat ttctgtagaa acacggatgt gcatgtgcag
     4441 attccctttt gcaggtatta aaaataatta aaaatagtcc tgcctgaggt tgcagtgagc
     4501 cgagcttgca ctactgcact ccagcctggg tgacagagta agactccatg tcaaaaaaaa
     4561 aaaaaaaaaa aaaaaagtcc tgccttaact aactcctctg cgcttgttca ctagtaacct
     4621 aaagaggcta tattcattct ttatgcaatg agggtatttt tgagtgaatt ttaactgctc
     4681 tgaactaagt ataagctcat gggcctgcaa aggttcagac ggtttctcct ttgcacccag
     4741 gaggaacttt ggctgcgaga atggggggat gtatccctca tgcagttggc atccaggcag
     4801 ccctctgcag cagcacaccc tgcaggcgga gttttcagag gatgcaattt tggatcccga
     4861 attttgatgt accttaaact tccacatcac tgcaccctga aacagagcat gctttccaga
     4921 aagtcacact ctcagatctg tgtcaagttc aatgtgagcc ctggcaaggc tggcatatta
     4981 acacctgcct tctggcttct gaaagtgaga tttgtatatg ggctgcactc acgcatatac
     5041 gagttggttt atctttgtgt acatgactat aacccagtga tgctgaggtc atgtgctgga
     5101 atgctgtatt tggaccacac atttcaaagt tgccctatgg aaatgaatcc tacttagtga
     5161 caagtcatca aatgtttgtc acatgtgatg aagacaaata tgtatacctg gcatagagaa
     5221 aaatatatac ctggtacatt ggagaaaaat aattacactt tcaaagagaa ttccctttgc
     5281 aattttatgt ttggatcacc actgtaagca cactttattt gcatttgatc tgtatttgta
     5341 tatgctgatg caatgataaa aatcactgta atacttcatt gtgttgtact ggatgcaaag
     5401 ctagaaaata ttgcaataaa tgagaccgat gaaagacttc tctgaaaa
//
