LOCUS       NM_017839               5313 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens lysophosphatidylcholine acyltransferase 2 (LPCAT2),
            mRNA.
ACCESSION   NM_017839
VERSION     NM_017839.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Murano,H., Inoue,S., Hashidate-Yoshida,T., Shindou,H., Shimizu,T.,
            Otaki,Y., Minegishi,Y., Kitaoka,T., Futakuchi,M., Igarashi,A.,
            Nishiwaki,M., Nemoto,T., Sato,M., Kobayashi,M., Sato,K., Hanawa,T.,
            Miyazaki,O. and Watanabe,M.
  TITLE     Lysophospholipid Acyltransferase 9 Promotes Emphysema Formation via
            Platelet-activating Factor
  JOURNAL   Am J Respir Cell Mol Biol 70 (6), 482-492 (2024)
   PUBMED   38377392
  REMARK    GeneRIF: Lysophospholipid Acyltransferase 9 Promotes Emphysema
            Formation via Platelet-activating Factor.
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Cao,N., Zhang,F., Yin,J., Zhang,J., Bian,X., Zheng,G., Li,N.,
            Lin,Y. and Luo,L.
  TITLE     LPCAT2 inhibits colorectal cancer progression via the PRMT1/SLC7A11
            axis
  JOURNAL   Oncogene 43 (22), 1714-1725 (2024)
   PUBMED   38605214
  REMARK    GeneRIF: LPCAT2 inhibits colorectal cancer progression via the
            PRMT1/SLC7A11 axis.
REFERENCE   3  (bases 1 to 5313)
  AUTHORS   Abate,W., Alrammah,H., Kiernan,M., Tonks,A.J. and Jackson,S.K.
  TITLE     Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises
            with TLR4 and regulates macrophage inflammatory gene expression in
            response to LPS
  JOURNAL   Sci Rep 10 (1), 10355 (2020)
   PUBMED   32587324
  REMARK    GeneRIF: Lysophosphatidylcholine acyltransferase 2 (LPCAT2)
            co-localises with TLR4 and regulates macrophage inflammatory gene
            expression in response to LPS.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5313)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5313)
  AUTHORS   Williams,K.A., Lee,M., Hu,Y., Andreas,J., Patel,S.J., Zhang,S.,
            Chines,P., Elkahloun,A., Chandrasekharappa,S., Gutkind,J.S.,
            Molinolo,A.A. and Crawford,N.P.
  TITLE     A systems genetics approach identifies CXCL14, ITGAX, and LPCAT2 as
            novel aggressive prostate cancer susceptibility genes
  JOURNAL   PLoS Genet 10 (11), e1004809 (2014)
   PUBMED   25411967
  REMARK    GeneRIF: three of these five genes (CXCL14, ITGAX, and LPCAT2)
            harbored polymorphisms associated with aggressive disease
            development in a human GWAS cohort consisting of 1,172 prostate
            cancer patients.
            Erratum:[PLoS Genet. 2015 May 01;11(5):e1005127. doi:
            10.1371/journal.pgen.1005127.. PMID: 25933217]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5313)
  AUTHORS   Casabonne,D., Reina,O., Benavente,Y., Becker,N., Maynadie,M.,
            Foretova,L., Cocco,P., Gonzalez-Neira,A., Nieters,A., Boffetta,P.,
            Middeldorp,J.M. and de Sanjose,S.
  TITLE     Single nucleotide polymorphisms of matrix metalloproteinase 9
            (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus
            in chronic lymphocytic leukemia: results from the European
            case-control study EpiLymph
  JOURNAL   Haematologica 96 (2), 323-327 (2011)
   PUBMED   21048031
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-environment interaction. (HuGE Navigator)
REFERENCE   7  (bases 1 to 5313)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   8  (bases 1 to 5313)
  AUTHORS   Agarwal,A.K. and Garg,A.
  TITLE     Enzymatic activity of the human
            1-acylglycerol-3-phosphate-O-acyltransferase isoform 11:
            upregulated in breast and cervical cancers
  JOURNAL   J Lipid Res 51 (8), 2143-2152 (2010)
   PUBMED   20363836
  REMARK    GeneRIF: Our enzymatic assays strongly suggest that the cDNA
            previously identified as LPCAT2/lyso platelet-activating
            factor-acetyltransferase cDNA has AGPAT activity and thus we prefer
            to identify this clone as AGPAT11 as well.
REFERENCE   9  (bases 1 to 5313)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 5313)
  AUTHORS   Shindou,H., Hishikawa,D., Nakanishi,H., Harayama,T., Ishii,S.,
            Taguchi,R. and Shimizu,T.
  TITLE     A single enzyme catalyzes both platelet-activating factor
            production and membrane biogenesis of inflammatory cells. Cloning
            and characterization of acetyl-CoA:LYSO-PAF acetyltransferase
  JOURNAL   J Biol Chem 282 (9), 6532-6539 (2007)
   PUBMED   17182612
  REMARK    GeneRIF: The enzyme acetyl-CoA:LYSO-PAF acetyltransferase catalyzes
            not only biosynthesis of PAF upon acute inflammatory stimulation
            but also incorporation of arachidonoyl-CoA to produce PAF precursor
            membrane glycerophospholipids for membrane biogenesis.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC002472.2, BX641069.1,
            BC005981.1, AW366682.1 and AI915099.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017839.4.
            
            Summary: This gene encodes a member of the lysophospholipid
            acyltransferase family. The encoded enzyme may function in two
            ways: to catalyze the biosynthesis of platelet-activating factor
            (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from
            1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the
            synthesis of glycerophospholipid precursors from arachidonyl-CoA
            and lysophosphatidylcholine. The encoded protein may function in
            membrane biogenesis and production of platelet-activating factor in
            inflammatory cells. The enzyme may localize to the endoplasmic
            reticulum and the Golgi. [provided by RefSeq, Feb 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.277298.1,
                                           SRR1660809.169265.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262134.10/ ENSP00000262134.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1861              BC002472.2         31-1891
            1862-2789           BX641069.1         1835-2762
            2790-2790           BC005981.1         597-597
            2791-4456           BX641069.1         2764-4429
            4457-4457           AW366682.1         517-517             c
            4458-5312           BX641069.1         4431-5285
            5313-5313           AI915099.1         12-12               c
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q12.2"
     gene            1..5313
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="lysophosphatidylcholine acyltransferase 2"
                     /db_xref="GeneID:54947"
                     /db_xref="HGNC:HGNC:26032"
                     /db_xref="MIM:612040"
     exon            1..281
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1745
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /EC_number="2.3.1.51"
                     /EC_number="2.3.1.23"
                     /EC_number="2.3.1.67"
                     /EC_number="2.3.1.25"
                     /note="acyl-CoA:lysophosphatidylcholine acyltransferase 2;
                     lyso-platelet-activating factor (PAF) acetyltransferase;
                     acetyl-CoA:lyso-PAF acetyltransferase; LPCAT-2; LPC
                     acyltransferase 2; acyltransferase-like 1; lysoPC
                     acyltransferase 2; 1-acylglycerophosphocholine
                     O-acyltransferase; 1-alkylglycerophosphocholine
                     O-acetyltransferase; 1-AGPAT 11; LPAAT-alpha; 1-AGP
                     acyltransferase 11; lysophosphatidic acid acyltransferase
                     alpha; 1-acylglycerol-3-phosphate O-acyltransferase 11;
                     acetyl-CoA:lyso-platelet-activating factor
                     acetyltransferase; 1-alkenylglycerophosphocholine
                     O-acyltransferase; lysophospholipid acyltransferase 9"
                     /codon_start=1
                     /product="lysophosphatidylcholine acyltransferase 2"
                     /protein_id="NP_060309.2"
                     /db_xref="CCDS:CCDS10753.1"
                     /db_xref="GeneID:54947"
                     /db_xref="HGNC:HGNC:26032"
                     /db_xref="MIM:612040"
                     /translation="MSRCAQAAEVAATVPGAGVGNVGLRPPMVPRQASFFPPPVPNPF
                     VQQTQIGSARRVQIVLLGIILLPIRVLLVALILLLAWPFAAISTVCCPEKLTHPITGW
                     RRKITQTALKFLGRAMFFSMGFIVAVKGKIASPLEAPVFVAAPHSTFFDGIACVVAGL
                     PSMVSRNENAQVPLIGRLLRAVQPVLVSRVDPDSRKNTINEIIKRTTSGGEWPQILVF
                     PEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCMLTFCQ
                     LFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQ
                     LTLPMEAGLVEFTKISRKLKLDWDGVRKHLDEYASIASSSKGGRIGIEEFAKYLKLPV
                     SDVLRQLFALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITE
                     EEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQT
                     CHVFSLPKEVQTTPSTASNKVSPEKHEESTSDKKDD"
     exon            282..421
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            422..639
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            640..752
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            753..813
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            814..872
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            873..907
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            908..962
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1045
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1171
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1325
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1424
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1560
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..5313
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3812..3817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="hexamer: CATAAA"
     regulatory      3828..3833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="hexamer: ATTAAA"
     polyA_site      3837
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="major polyA site"
     polyA_site      3851
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="major polyA site"
     regulatory      5292..5297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="hexamer: ATTAAA"
     polyA_site      5313
                     /gene="LPCAT2"
                     /gene_synonym="AGPAT11; AYTL1; LPLAT9; LysoPAFAT"
                     /note="major polyA site"
ORIGIN      
        1 agaggctccc cagcgtcgcc ctaggctggg actctagtag gtcttcggct cagttttggc
       61 tgcagcgccc gcgtagatcg cttcggccgg gttctacgcc cggctcaact atgagccggt
      121 gcgcccaggc ggcggaagtg gcggccacag tgccaggtgc cggcgtcggg aacgtggggc
      181 tgcggccgcc catggtgccc cgtcaggcgt ccttcttccc gccgccggtg ccgaacccct
      241 tcgtgcagca gacgcagatc ggctccgcga ggcgggtcca gattgtcctt cttgggatta
      301 tcttgcttcc aattcgtgtc ttattggttg cgttaatttt attacttgca tggccatttg
      361 ctgcaatttc aacagtatgc tgtcctgaaa agctgaccca cccaataact ggttggagga
      421 ggaaaattac tcaaacagct ttgaaatttc tgggtcgtgc tatgttcttt tcaatgggat
      481 ttatagttgc tgtaaaagga aagattgcaa gtcctttgga agcaccagtt tttgttgctg
      541 cccctcattc aacattcttt gatggaattg cctgtgttgt agctgggtta ccttctatgg
      601 tatctcgaaa tgagaatgca caagtccctc tgattggcag actgttacgg gctgtgcaac
      661 cagttttggt gtcccgtgta gatccggatt cccgaaaaaa cacaataaat gaaataataa
      721 agcgaacaac atcaggagga gaatggcccc agatactagt tttcccagaa ggtacttgta
      781 ctaatcgttc ctgtttgatt acttttaaac caggagcctt cattccagga gttccagtgc
      841 agccagtcct cctcagatac ccaaacaagc tggatactgt gacctggaca tggcaaggat
      901 atacattcat tcagctttgt atgcttactt tctgccagct cttcacaaag gtagaagttg
      961 agtttatgcc agttcaagta ccaaatgatg aagaaaaaaa tgatcctgtc ctttttgcca
     1021 ataaagtccg gaatttaatg gcagaagctc tgggaatacc agtaacagat catacctatg
     1081 aagactgcag attgatgatt tcagcaggac agctaacatt gcctatggaa gctgggctgg
     1141 tggaatttac taaaattagc cgaaaattga aattagattg ggatggtgtt cgtaagcatt
     1201 tggatgaata tgcatctatt gcgagttcct caaaaggagg aagaattgga attgaagaat
     1261 tcgccaagta tttaaagttg cctgtttcag atgtcttgag acaacttttt gcactctttg
     1321 acaggaacca tgatggcagc attgacttcc gagagtatgt gattggcctg gctgtcttgt
     1381 gcaacccttc caacacagag gagatcatcc aggtggcatt taagctgttt gacgttgatg
     1441 aggatggcta cataacggag gaagagttct ccaccattct acaggcttcc cttggagtgc
     1501 ctgaccttga tgtttctggt ctcttcaagg aaatagccca aggggactca atttcctatg
     1561 aggaatttaa aagttttgcc ttaaagcatc cagaatatgc taagatattt acaacatacc
     1621 tagacctcca gacgtgccat gtgttttcat taccaaaaga agtccagaca accccctcca
     1681 ccgccagtaa taaagtcagc cctgaaaagc atgaagagag tacctcagac aaaaaagatg
     1741 actgaaagca gtatttccaa taaggaaaac acagtagctt ttgcttgaaa ttgtaaaggc
     1801 acttattgat aatactttta atgtgttggt aatgatgttt aaaattgaaa gatttttaaa
     1861 acaaaaatga tagattttct tactaaaaat gtttttatta accttgcttt tattggaaaa
     1921 aatcaagcaa tatttcgttt tcttttgtgt tatattgtac tttactgatt catttactgg
     1981 tgatacatat gtttttatgg attttccagt ttaatttgca tatacaaatg aatgcaatgg
     2041 tctattggtg agcattgagc aacactgtat aaagttttaa aaatgtaaac actttttaat
     2101 ctactttcct ctaaaaatca ataatattct attatttcta atccttttcc acttgggaaa
     2161 taacaatgaa gaatctgaga atttgacatc tataacttta cagattcatt tttccattta
     2221 aatttcagtt tcttggatca ctgaatatgg gaagggagag cttcactaat tagacgcagc
     2281 ttcttaagaa cttatattct ctttgacata catctctatt gtagtttttt gttttgtttt
     2341 gttttttgag atggagtctt gctctgtcac ccaggctgga gtgcagtggt gcaatctcag
     2401 ctcactgcaa cctctgcctc ctgggttcaa gtgattctcg tacctcagac tcccgagtag
     2461 ttgggattac aggtgcccac caccacaccc gactaatttt tgtattttta gtagccatgt
     2521 ggttttgcca tgttggccag gctggtttcg aactcctgac ctcaggtgat ccacccacct
     2581 cagcctccca aagtgctggg attacaggtg tgagccaccg ttcccggcct attgtagatt
     2641 ttcttaacta cttgctaagg aaatcatatc cttttacatg aactacaggt ttagaacttg
     2701 gttttaagac aactgctatg gccagaaggt aaatgggaat tgccttattg aaggtaacat
     2761 tgattgccta ataagaaaat gaattgtttg ccacagagtt gaatttaatt tgagttagat
     2821 agttcagaat gtagcacttg ccctataaat gaatcagatt tgttctattt atataatatt
     2881 agaattaata tattatcatg taagtgggaa ttttattttg ttaagtggac tctcaaattt
     2941 tagaacttgt gtgaaatatc tcccagaaaa cacaaaaggt ttcaaggtat cacccaaagc
     3001 tagggaatca acaaacgtac tttattaatg caagaccaca atttaagccc caggcaggaa
     3061 tccaacagta acatttttcc tttaggttaa gcagtaattt ttttgaattg tcatttttaa
     3121 tgggtctcac acatgcatat tgctaatatc attcatgttt gaggttttca acaaagaacc
     3181 aatttattaa tataatgtac aaaaatagtt ggttcatttt ctgaattcag tctttgaaat
     3241 aaaactcctt gttttggccg tgtttctgaa tgtattggtg attcaccccc aagctaattt
     3301 tttaaagtca tttttgaagt tgggaagtct catgagagat gttgaagtat gtatttaatc
     3361 aagagtcatg atttcaaact agttttacat attaagcagt tagtgttcta atttaatggg
     3421 taaatgtgtg tttggataaa tatctgaaaa ttttatcctt aagtatatat aattatttgc
     3481 ctcttatatg tcttaaagct atttaaacaa ggtgttaaat gagccaaaac aattaagtaa
     3541 ttagaacagt acatttttat cagagtgtct gtcatatgca atgaatgtat gtaatactaa
     3601 aaaatcatga ctacttttat caaagaaaaa ccacacatta atcctattaa tcatgaaagc
     3661 gtagcattgt aaattaaagg ttttctttga ggctcttgaa agtgatccca ttgctttcct
     3721 gttttaaaaa tattttatgc tctttatttc cacttctgtg aatgtgatat ttctattttg
     3781 tgattatgtt actgaataaa caaacttgct acataaaatt cttagcaatt aaaaaaattc
     3841 tgattctgcc attttgtctc aaatgtaata taccctttta atatcatatg tttacatatt
     3901 tatgaatgat taatcatttt tgtttgcatt aaactttatg aaatgtcaga aatgatttta
     3961 ctctaatgaa actcaaattt tgcccaaagt agcaatattc ttaaagatgt tgaattttga
     4021 ctggttgaat tttatatagt aacacacaag tcgtgatcat caatattatt gcagaccagg
     4081 actctgctta tatgccttgc taacaaagat ttctttcaca gaggcttttc tcctctcaag
     4141 acttaaagta agaattacat tttattaagt cagttaaatg caccatggct tcatatagta
     4201 atataaaaaa actctttgaa gtgagaaata ttatatccta aaacctctaa accacaaaca
     4261 ttcaattgaa agagttctgt tgaaggataa tgacatactg actgcttaca gaccaagttg
     4321 cttgcatttt gtatgtttag ccctcctttg ccactgcttt tagagccttg gaaggctaag
     4381 tgtgatagta atgctagctc taatgcatat ttaaaggaga ctgcctcgct tttagaagac
     4441 atctggtctg ctctctgcat gaggcacagc agtaaagctc tttgattccc agaatcaaga
     4501 actctcccct tcagactatt accgaatgca aggtggttaa ttgaaggcca ctaattgatg
     4561 ctcaaataga aggatattga ctatattgga acagatggag tctctactac aaaagtcttt
     4621 gggtatttgt ttcttacata gaaaatgcta acatgaatag aaagatactg gtgcaagacc
     4681 attcccggga aagtagacat acttacattt ttttcctttt ctgctcattt gaatgaactc
     4741 aattttatgt taaattgtta tatcagagtg acaaataagt gctatggctt gatagaagtg
     4801 aagctcttca catatattca aaatacatat cacaaacttt ggtaaatagg atagtaatct
     4861 gaagaacttt tgcccttttt accccattta ctgtaactct tgtttctagg taatcgttct
     4921 ctctcaacaa acttctcaag cgtctgtgta acaagccaca tgttctaaca aattgtctcc
     4981 atcgcacttc aacagccagg tccctatttt ttataacgta ttaaccttat tattttctta
     5041 ttattttaaa agaatctatg cacattagca aaatttaaaa gatagagaaa aatataaaca
     5101 gaaaaaatta tgtttacttc taccacccta aatcaactat tatcaatttt atacatattt
     5161 tactccatct tttttcaaag tttcttacat tttccaatgt cattaaaatt ctctgtgaat
     5221 gtaaatttta aaaactgtac ctactgtttt ttggaaatct gtaacaagct atgtgtaatc
     5281 attcttctaa tattaaacat attgtgtcca gaa
//
