LOCUS       NM_031490               8195 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens lon peptidase 2, peroxisomal (LONP2), transcript
            variant 1, mRNA.
ACCESSION   NM_031490
VERSION     NM_031490.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 8195)
  AUTHORS   Wu,W., Liu,F., Wu,K., Chen,Y., Wu,H., Dai,G. and Zhang,W.
  TITLE     Lon Peptidase 2, Peroxisomal (LONP2) Contributes to Cervical
            Carcinogenesis via Oxidative Stress
  JOURNAL   Med Sci Monit 24, 1310-1320 (2018)
   PUBMED   29502128
  REMARK    GeneRIF: Findings suggest that LONP2 promotes cervical
            tumorigenesis via oxidative stress.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8195)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 8195)
  AUTHORS   Boldt,K., van Reeuwijk,J., Lu,Q., Koutroumpas,K., Nguyen,T.M.,
            Texier,Y., van Beersum,S.E., Horn,N., Willer,J.R., Mans,D.A.,
            Dougherty,G., Lamers,I.J., Coene,K.L., Arts,H.H., Betts,M.J.,
            Beyer,T., Bolat,E., Gloeckner,C.J., Haidari,K., Hetterschijt,L.,
            Iaconis,D., Jenkins,D., Klose,F., Knapp,B., Latour,B.,
            Letteboer,S.J., Marcelis,C.L., Mitic,D., Morleo,M., Oud,M.M.,
            Riemersma,M., Rix,S., Terhal,P.A., Toedt,G., van Dam,T.J., de
            Vrieze,E., Wissinger,Y., Wu,K.M., Apic,G., Beales,P.L.,
            Blacque,O.E., Gibson,T.J., Huynen,M.A., Katsanis,N., Kremer,H.,
            Omran,H., van Wijk,E., Wolfrum,U., Kepes,F., Davis,E.E., Franco,B.,
            Giles,R.H., Ueffing,M., Russell,R.B. and Roepman,R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8195)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   7  (bases 1 to 8195)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 8195)
  AUTHORS   Omi,S., Nakata,R., Okamura-Ikeda,K., Konishi,H. and Taniguchi,H.
  TITLE     Contribution of peroxisome-specific isoform of Lon protease in
            sorting PTS1 proteins to peroxisomes
  JOURNAL   J Biochem 143 (5), 649-660 (2008)
   PUBMED   18281296
REFERENCE   9  (bases 1 to 8195)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   10 (bases 1 to 8195)
  AUTHORS   Kikuchi,M., Hatano,N., Yokota,S., Shimozawa,N., Imanaka,T. and
            Taniguchi,H.
  TITLE     Proteomic analysis of rat liver peroxisome: presence of
            peroxisome-specific isozyme of Lon protease
  JOURNAL   J Biol Chem 279 (1), 421-428 (2004)
   PUBMED   14561759
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ548761.1, AC023818.5 and
            BX104260.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_031490.4.
            
            Summary: In human, peroxisomes function primarily to catalyze fatty
            acid beta-oxidation and, as a by-product, produce hydrogen peroxide
            and superoxide. The protein encoded by this gene is an
            ATP-dependent protease that likely plays a role in maintaining
            overall peroxisome homeostasis as well as proteolytically degrading
            peroxisomal proteins damaged by oxidation. The protein has an
            N-terminal Lon N substrate recognition domain, an ATPase domain, a
            proteolytic domain, and, in some isoforms, a C-terminal peroxisome
            targeting sequence. Alternative splicing results in multiple
            transcript variants encoding distinct isoforms. [provided by
            RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ548761.1, SRR1803614.211694.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285737.9/ ENSP00000285737.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4086              AJ548761.1         1-4086
            4087-4723           AC023818.5         75674-76310         c
            4724-4825           BX104260.1         321-422
            4826-8195           AC023818.5         72202-75571         c
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q12.1"
     gene            1..8195
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /note="lon peptidase 2, peroxisomal"
                     /db_xref="GeneID:83752"
                     /db_xref="HGNC:HGNC:20598"
                     /db_xref="MIM:617774"
     exon            1..322
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..2648
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /EC_number="3.4.21.53"
                     /note="isoform 1 is encoded by transcript variant 1;
                     peroxisomal LON protease like; peroxisomal Lon protease
                     homolog 2; lon protease homolog 2, peroxisomal; lon
                     protease 2; lon protease-like protein 2"
                     /codon_start=1
                     /product="lon protease homolog 2, peroxisomal isoform 1"
                     /protein_id="NP_113678.2"
                     /db_xref="CCDS:CCDS10734.1"
                     /db_xref="GeneID:83752"
                     /db_xref="HGNC:HGNC:20598"
                     /db_xref="MIM:617774"
                     /translation="MSSVSPIQIPSRLPLLLTHEGVLLPGSTMRTSVDSARNLQLVRS
                     RLLKGTSLQSTILGVIPNTPDPASDAQDLPPLHRIGTAALAVQVVGSNWPKPHYTLLI
                     TGLCRFQIVQVLKEKPYPIAEVEQLDRLEEFPNTCKMREELGELSEQFYKYAVQLVEM
                     LDMSVPAVAKLRRLLDSLPREALPDILTSIIRTSNKEKLQILDAVSLEERFKMTIPLL
                     VRQIEGLKLLQKTRKPKQDDDKRVIAIRPIRRITHISGTLEDEDEDEDNDDIVMLEKK
                     IRTSSMPEQAHKVCVKEIKRLKKMPQSMPEYALTRNYLELMVELPWNKSTTDRLDIRA
                     ARILLDNDHYAMEKLKKRVLEYLAVRQLKNNLKGPILCFVGPPGVGKTSVGRSVAKTL
                     GREFHRIALGGVCDQSDIRGHRRTYVGSMPGRIINGLKTVGVNNPVFLLDEVDKLGKS
                     LQGDPAAALLEVLDPEQNHNFTDHYLNVAFDLSQVLFIATANTTATIPAALLDRMEII
                     QVPGYTQEEKIEIAHRHLIPKQLEQHGLTPQQIQIPQVTTLDIITRYTREAGVRSLDR
                     KLGAICRAVAVKVAEGQHKEAKLDRSDVTEREGCREHILEDEKPESISDTTDLALPPE
                     MPILIDFHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPLGGEIMFVEASRMDGEGQ
                     LTLTGQLGDVMKESAHLAISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPAGAVTKD
                     GPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKQV
                     IIPRRNEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFTVKTRPGLLNSKL"
     exon            323..557
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            558..689
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            690..812
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            813..976
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1071
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1330
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1472
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1623
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1750
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1884
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2027
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2235
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2426
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..8195
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6328..6333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /note="hexamer: AATAAA"
     polyA_site      6350
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /note="major polyA site"
     regulatory      7857..7862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
                     /note="hexamer: AATAAA"
     polyA_site      7879
                     /gene="LONP2"
                     /gene_synonym="LONP; LONPL; PLON; PSLON"
ORIGIN      
        1 gggtctgagg tttggtgact gcggggcagg ccgggggcag ctgtctgtct ggctcttttt
       61 gacagccccc agtgcgaaag gctgccagca tgtcatcagt gagccccatc cagatcccca
      121 gtcgcctccc gctgctgctc acccacgagg gcgtcctgct gcccggctcc accatgcgca
      181 ccagcgtgga ctcggcccgc aacctgcagc tggtgcggag ccgccttctg aagggcacgt
      241 cgctgcaaag caccatcctg ggcgtcatcc ccaacacgcc tgaccccgcc agcgacgcgc
      301 aggacctgcc gccgctgcac aggattggca cagctgcact ggccgttcag gttgtgggca
      361 gtaactggcc caagccccac tacactctgt tgattacagg cctatgccgt ttccagattg
      421 tacaggtctt aaaagagaag ccatatccca ttgctgaagt ggagcagttg gaccgacttg
      481 aggagtttcc caacacctgt aaaatgaggg aggagctagg agaactatca gagcagtttt
      541 acaaatatgc agtacaattg gttgaaatgt tggatatgtc tgtccctgca gttgctaaat
      601 tgagacgtct tttagatagt cttccaaggg aagctttacc agacatcttg acatcaatta
      661 tccgaacaag caacaaagag aaactccaga ttttagatgc tgtgagccta gaggagcggt
      721 tcaagatgac tataccactg cttgtcagac aaattgaagg cctgaaattg cttcaaaaaa
      781 ccagaaaacc caagcaagat gatgataaga gggttatagc aatacgccct attaggagaa
      841 ttacacatat ctcaggtact ttagaagatg aagatgaaga tgaagataat gatgacattg
      901 tcatgctaga gaaaaaaata cgaacatcta gtatgccaga gcaggcccat aaagtctgtg
      961 tcaaagagat aaagagactc aaaaaaatgc ctcagtcaat gccagaatat gctctgacta
     1021 gaaattattt ggaacttatg gtagaacttc cttggaacaa aagtacaact gaccgcctgg
     1081 acattagggc agcccggatt cttctggata atgaccatta cgccatggaa aaattgaaga
     1141 aaagagtact ggaatacttg gctgtcagac agctcaaaaa taacctgaag ggcccaatcc
     1201 tatgctttgt tggccctcct ggagttggta aaacaagtgt gggaagatca gtggccaaga
     1261 ctctaggtcg agagttccac aggattgcac ttggaggagt atgtgatcag tctgacattc
     1321 gaggacacag gcgcacctat gttggcagca tgcctggtcg catcatcaac ggcttgaaga
     1381 ctgtgggagt gaacaaccca gtgttcctat tagatgaggt tgacaaactg ggaaaaagtc
     1441 tacagggtga tccagcagca gctctgcttg aggtgttgga tcctgaacaa aaccataact
     1501 tcacagatca ttatctaaat gtggcctttg acctttctca agttcttttt atagctactg
     1561 ccaacaccac tgctaccatt ccagctgcct tgttggacag aatggagatc attcaggttc
     1621 caggttatac acaggaggag aagatagaga ttgcccatag gcacttgatc cccaagcagc
     1681 tggaacaaca tgggctgact ccacagcaga ttcagatacc ccaggtcacc actcttgaca
     1741 tcatcaccag gtataccaga gaggcagggg ttcgttctct ggatagaaaa cttggggcca
     1801 tttgccgagc tgtggccgtg aaggtggcag aaggacagca taaggaagcc aagttggacc
     1861 gttctgatgt gactgagaga gaaggttgca gagaacacat cttagaagat gaaaaacctg
     1921 aatctatcag tgacactact gacttggctc taccacctga aatgccgatt ttgattgatt
     1981 tccatgctct gaaagacatc cttgggcccc cgatgtatga aatggaggta tctcagcgtt
     2041 tgagtcagcc aggagtagca ataggtttgg cttggactcc cttaggtgga gaaatcatgt
     2101 tcgtggaggc gagtcgaatg gatggcgagg gccagttaac tctgaccggc cagctcgggg
     2161 acgtgatgaa ggagtccgcc cacctcgcta tcagctggct ccgcagcaac gcaaagaagt
     2221 accagctgac caatgctttt ggaagttttg atcttcttga caacacagac atccatctgc
     2281 acttcccagc tggagctgtc acaaaagatg gaccatctgc tggagttacc atagtaacct
     2341 gtctcgcctc actttttagt gggcggctgg tacgttcaga tgtagccatg actggagaaa
     2401 ttacactgag aggtcttgtt cttccagtgg gtggaattaa agacaaagtg ctggcggcac
     2461 acagagcggg actgaagcaa gtcattattc ctcggagaaa tgaaaaagac cttgagggaa
     2521 tcccaggcaa cgtacgacag gatttaagtt ttgtcacagc aagctgcctg gatgaggttc
     2581 ttaatgcagc ttttgatggt ggctttactg tcaagaccag acctggtctg ttaaatagca
     2641 aactgtaggt ccaaatctca attttttaga attttaagtt atgaagtgct caaaggtact
     2701 gacacagttg attttattca caccattagg ggtatgcaag atgtccctgt tttataaaca
     2761 taatcacaac agtaataaac ctcaagtagt ggctagtgtt tagtatagaa atataagatg
     2821 ttgatttagt aaactgataa aaatcgaatt cttgtctttt tagtgggatc cttactgtcc
     2881 ctggaaagat atagcatagt ggttctcagc acagtctcca gaacagaagc atctgtagta
     2941 cctggtaact tgttagaaat gtacattctc aggctccaca gcaggccgcc tgaatcaaat
     3001 cctgggaggt ggggacagaa atctgtgttt taagaagcct tccaggtaat tctgctgcac
     3061 actcaagttc aggaaccacc ggtatagacc attaccttag tggatttacc tgtagagttt
     3121 attggatcct gaaaccaatc aattacttag aactaggcaa agatgaaagt atagccaact
     3181 attcttggct atatatatat attcaagtgg gccgggcgtg atggctcaca cctgtaattc
     3241 cagcactttg ggaggtcgag gtaggcagat caccgagccc aagagttcaa gacaatcctg
     3301 gccaacggcg aaactctgtc tctacaaaaa atatacaggc gtgttagcat gtgcctgtaa
     3361 tcccagcttc ttgggaagct gaggcacaag aattgcctga acccaggagg tggaggttgc
     3421 agtgagctgg gatcgcgcca ttgcactcca gcctggctga cagagcgaga ctgtctctaa
     3481 aaaaaaaaga ctcaagtgga ccctacaatg aagcctacac atcccaatag aagccccttc
     3541 ttatgctgag ggaagcagcc ctcagaacat gatagcttgt atccagcaga gtggcacgtg
     3601 ctggcacacc tcacagaagc accctggccc tggatgcctg caacctcaga agagtgcagc
     3661 tcccagaggg aggcagccat ccatctggga tggtcctaag catggaatcc taactcctga
     3721 ttccgtctcc tatttcttgc ttggctacgc cagttcccaa atctggtaga tgtccatgcc
     3781 catgtgctcc tgctgggact caattcaggc tatgtatgac tatgaagtca ggctcatctg
     3841 cttactggct gtgtgaactt tttgtatctt ggttttcttc atccatgaaa tccaagtaat
     3901 actacctaat tgttactgtg gagattaagt tcaaatgcaa tgtatagtaa tattaagcaa
     3961 tttctagtta ttattctagc cagtaatgga cttcagaatc ttttattaca caatataaga
     4021 atatgtatgt aaagacattt tggaatttcc tggatgagaa ggaagtctgg gctgggcatg
     4081 gtggctcacg cctgtaaccc tagcacttta ggaaatcgag gcgagtggat cacttaagct
     4141 caggagttca aggccagcct gggcaacatg gcaaaacccc atttctacaa aaaatacaaa
     4201 aattagctgg gcatggtggc acccgcctgt agtccagcta cttgaggctg agatgggagg
     4261 atgagggagg tcggggctgc agtgagccaa gatcacgcca ctgcactcca gcaccctggg
     4321 cgacagagtg agaccctgtc tcaaaaaaaa aaaaaaaaaa aaagattggg ccaaaatact
     4381 gtgataaaat agcaggcctg ctgataaaag tttatctgaa tgcattgaga ggaaaagtcc
     4441 agacctagga ctagttatgg cagttggaga gaaagaacat cgggatgttt gaaaatatgc
     4501 cattgactat cttaactact gtaattttat catttccaac gtcatctaac tggggactag
     4561 aacaaactgt gaattcactt tcagcaacca gagggcgcta atccacaccc acatcgctct
     4621 gccctgttcc acccagcagg ggcaacaagg atataacttg gggttctcgg tattcttcct
     4681 ttagtcctga cacaggcagc cttgcacttt gtagcagcag gagggcactt gctttaagca
     4741 tatctttcga aaggcatcca ttgagagaat aatgcattct ccccttgctg tgtatgacat
     4801 ggaacagtat gaccattgca ctagctttgt ttttggtttg ttttgttttt tttttaattc
     4861 caggggtggg aggttggttg gttgaagtct aagctcttcc tttttcacct gggttttttt
     4921 tttttggggg gggtgggggg ttaggggtgg ggcgggtggg gaagagccaa aattttaacc
     4981 atttttaagc atataatttg ggggtatcag ttactggatc taagcatgtc cactctacac
     5041 gctttttttt tttttttttt tttttttttt tgagatggag tcttgctctg ttacccaggc
     5101 tgcactgcag tggcatgatc tcggctcact gcaacctctg cctccccagt tcaagcaatt
     5161 ctcatgcctc agcctcccaa gtagctggga ccacaggtgt gtgccaccac gcccagctaa
     5221 tttttgtatt ttttagtaga gacagggttt caccatgttg gccaggctgg tctcaaactc
     5281 ccaacctcaa atgatccggc caccttggcc tcccaaaatg ctggcattac aggcgtgagc
     5341 caccacaccc ggcttacatg cattttttat caccacaaac tgaaactctg tacccactga
     5401 aaaagagctc caaattccac cctcacccca gaccctggta accactattt cactttcttt
     5461 ctgaatttcc cttcatatga gtgggatcaa acaagagttg tccttttgtg tttagcattc
     5521 ttacttagca agccatcaac tgctcgaaca gtcactgggg caactacttt tcgcccagtg
     5581 tcctccagaa aagaaagatc tggagggtca ggccactctt tttcttcaaa ataatggggt
     5641 gcagaagggt agggatgaac ctcttcctcc tttgccactt tagctttagc tcaaaggaac
     5701 tccaactttt tattatttgc aacagttagc tttctttgtt tcacatctct gccttgcaga
     5761 accatgacct atcaaggtgt tttgtcaaac aaaaactaat actttccctg ggaaaaagcc
     5821 atgtggtagc aagtaccaga aatttttatg gccaaataat acaccactat atccatagac
     5881 cgcatttggt ttatccactc atctgctgat tgtttccccc tcttgggtat tatgagtaat
     5941 gctatgaaca tgggtgtaca catctctctt gaagtccctt cttaggtcct ttagggatac
     6001 atatccagga gcagaactac tagatcacat atgctaaggt ctgaatgtcc ccccaccaaa
     6061 ttcgtatgtt gaagcaacca ccaacgtgat aggtgaggct ttaggaggtg attatgtcat
     6121 gagagctcca tcctcattaa tggatttagt gcctttataa aggggctcaa gggaaccaat
     6181 tacctccttt ttgcccttcg attcccttcc accatatgag gacacagcaa caggccccgt
     6241 cctagaagct gagactgggc cctcaataga tgataccagt ctgccagcac gctgatcgtg
     6301 ggcttctcag tcttcacaac tatgagaaat aaattcgtac ttttaaatta cccagtctca
     6361 ggtattctgt tacggttaac acaaatgact aagacaacag gataattctg tttaactttt
     6421 tgagaactgc caaacttttc caccgcagct gcaccatttg acattccttc cagcaatgca
     6481 caaatgttcc agtttctcca catccttgcc agcactcatt ttctgggttt tttataatag
     6541 ccctccaaat gggtgtgacg tattttgatt ctatgtccct aatgactagt gatgttgagc
     6601 attttctagc attgattttt aagatgttac ccaaagaccc cttgtatcaa aataagctgg
     6661 atttttttat tgaaaattat taactctaga aattttagtt taaactagac ttagggatat
     6721 gtgtatttta ccggtattcc acgttttatg catgggtttt taaaacttct caagtattaa
     6781 aactaaaagc tttaggtgct ttgcttatca agaaatccta cactgtccac tggagacatc
     6841 catgttttta cttggctctg cccctttagt ggtccctgtg aaccttacct caaaccatgc
     6901 atctggggca gagatcctta cttgcttggt ggttacaaat gcaaatacag tgaagaatgt
     6961 catctttgtg attgttcctg aaatagttca cgagaaatcc atgaccgtaa agtactgtga
     7021 tagtgatgtc taccactgtg agcttccagt actaggtgat tggtctgcat tcacagtgac
     7081 caaaatcagc tatgtggcca ggtaattcac tgctgagggc tttggatttt cctttatgaa
     7141 ctactgaaat gaggtcaact tgactattac taagggacat tttgctacaa agaatgttag
     7201 ttttgccaat tccctttcca aatctaaaat ttattttaac caggatttta gatgtaaaca
     7261 tcaagtagtt ttggttgttt caatgaagta acatgtttaa gctcacatta tttgaagtac
     7321 ttcagttcct attgccatga aaattgtatc cagcagctaa aaaaaaaaaa aaaaaaaaag
     7381 actacagtta gtcattatcc aatttgatga tttatggtcc aacactaatg ctcatttttt
     7441 ttgtttgttt tacaaacatt tggtggatac cacaatgaaa actgcactta aaaaacaaaa
     7501 atgctgaaag aggaaggaaa tatcaaaaag gtctgaatag acaacaggca aatatggtga
     7561 gtggtcattg agatgtttta aaagttacag caaaaggact tctaaaacaa ttttaggaaa
     7621 agctttgtca tgaaaattca tttctttgga aatactgtag tttgtactta atgacactgt
     7681 cagcacattt ctaggcaata caaactcttg aggctaaatc ctcatcctga catgacagtg
     7741 caagcctgtc aaaatgtgac cccaaaacca ggtgtccttt tgctccatta tttatatctg
     7801 taaacctgtt attattttca gaattcagaa aggcctaaga aaattacatc tatgataata
     7861 aagtgtattt ctttgtcaag tttctagagc tatataagga tagcgaaaat atttggttca
     7921 gcaagacact ggggtatcaa gtcaggcata ataagcactt tatagcacat ctagccttcc
     7981 tcattccctt acgcagtgga catcataccc ttttgtggag gagggaccag gcagagaagg
     8041 tatttaaata aaaactggta gatacagagt cagaagtcaa cccaggcagt tagaatctac
     8101 aacccacact gtttgccacc aaatcttagt agatgcttga taaggaattt tgtttggatg
     8161 gtgccatttt tagataaacg tacgtttctt ttggt
//
