LOCUS       NM_175920              12383 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens leucyl and cystinyl aminopeptidase (LNPEP), transcript
            variant 2, mRNA.
ACCESSION   NM_175920
VERSION     NM_175920.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Marusina,A.I., Ji-Xu,A., Le,S.T., Toussi,A., Tsoi,L.C., Li,Q.,
            Luxardi,G., Nava,J., Downing,L., Leal,A.R., Kuzminykh,N.Y.,
            Kruglinskaya,O., Bruggen,M.C., Adamopoulos,I.E., Merleev,A.A.,
            Gudjonsson,J.E. and Maverakis,E.
  TITLE     Cell-Specific and Variant-Linked Alterations in Expression of
            ERAP1, ERAP2, and LNPEP Aminopeptidases in Psoriasis
  JOURNAL   J Invest Dermatol 143 (7), 1157-1167 (2023)
   PUBMED   36716917
  REMARK    GeneRIF: Cell-Specific and Variant-Linked Alterations in Expression
            of ERAP1, ERAP2, and LNPEP Aminopeptidases in Psoriasis.
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   Khaliq,O.P., Konoshita,T., Moodley,J. and Naicker,T.
  TITLE     The role of LNPEP and ANPEP gene polymorphisms in the pathogenesis
            of pre-eclampsia
  JOURNAL   Eur J Obstet Gynecol Reprod Biol 252, 160-165 (2020)
   PUBMED   32619880
  REMARK    GeneRIF: The role of LNPEP and ANPEP gene polymorphisms in the
            pathogenesis of pre-eclampsia.
REFERENCE   3  (bases 1 to 12383)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 12383)
  AUTHORS   Liu,W.H., Fang,Y.N., Wu,C.C., Chen,M.C., Chang,J.P., Lin,Y.S.,
            Pan,K.L., Ho,W.C., Chang,T.H., Huang,Y.K., Fang,C.Y., Chen,C.J. and
            Lee,W.C.
  TITLE     Differential Gene Expression Profile of Renin-Angiotensin System in
            the Left Atrium in Mitral Regurgitation Patients
  JOURNAL   Dis Markers 2018, 6924608 (2018)
   PUBMED   30581499
  REMARK    GeneRIF: LNPEP expression in the left atrium in mitral
            regurgitation patients significantly differed from those in aortic
            valve disease patients and normal controls.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12383)
  AUTHORS   Mpakali,A., Saridakis,E., Harlos,K., Zhao,Y., Kokkala,P.,
            Georgiadis,D., Giastas,P., Papakyriakou,A. and Stratikos,E.
  TITLE     Ligand-Induced Conformational Change of Insulin-Regulated
            Aminopeptidase: Insights on Catalytic Mechanism and Active Site
            Plasticity
  JOURNAL   J Med Chem 60 (7), 2963-2972 (2017)
   PUBMED   28328206
  REMARK    GeneRIF: This study describes a crystal structure of
            insulin-regulated aminopeptidase in complex with a recently
            developed bioactive and selective inhibitor at 2.53 A resolution.
REFERENCE   6  (bases 1 to 12383)
  AUTHORS   Itoh,C., Watanabe,M., Nagamatsu,A., Soeda,S., Kawarabayashi,T. and
            Shimeno,H.
  TITLE     Two molecular species of oxytocinase (L-cystine aminopeptidase) in
            human placenta: purification and characterization
  JOURNAL   Biol Pharm Bull 20 (1), 20-24 (1997)
   PUBMED   9013800
REFERENCE   7  (bases 1 to 12383)
  AUTHORS   Rogi,T., Tsujimoto,M., Nakazato,H., Mizutani,S. and Tomoda,Y.
  TITLE     Human placental leucine aminopeptidase/oxytocinase. A new member of
            type II membrane-spanning zinc metallopeptidase family
  JOURNAL   J Biol Chem 271 (1), 56-61 (1996)
   PUBMED   8550619
REFERENCE   8  (bases 1 to 12383)
  AUTHORS   Tsujimoto,M., Mizutani,S., Adachi,H., Kimura,M., Nakazato,H. and
            Tomoda,Y.
  TITLE     Identification of human placental leucine aminopeptidase as
            oxytocinase
  JOURNAL   Arch Biochem Biophys 292 (2), 388-392 (1992)
   PUBMED   1731608
REFERENCE   9  (bases 1 to 12383)
  AUTHORS   Mizutani,S., Akiyama,H., Kurauchi,O., Taira,H., Narita,O. and
            Tomoda,Y.
  TITLE     In vitro degradation of angiotensin II (A-II) by human placental
            subcellular fractions, pregnancy sera and purified placental
            aminopeptidases
  JOURNAL   Acta Endocrinol (Copenh) 110 (1), 135-139 (1985)
   PUBMED   3898693
REFERENCE   10 (bases 1 to 12383)
  AUTHORS   Beckman,L., Bjorling,G. and Christodoulou,C.
  TITLE     Pregnancy enzymes and placental polymorphism. II. Leucine
            aminopeptidase
  JOURNAL   Acta Genet Stat Med 16 (2), 122-131 (1966)
   PUBMED   5953194
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008850.7, U62769.1,
            AC008865.3, U62768.1, BC028692.1, BQ923659.1 and BG622952.1.
            
            On May 31, 2019 this sequence version replaced NM_175920.3.
            
            Summary: This gene encodes a zinc-dependent aminopeptidase that
            cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin,
            dynorphin A and other peptide hormones. The protein can be secreted
            in maternal serum, reside in intracellular vesicles with the
            insulin-responsive glucose transporter GLUT4, or form a type II
            integral membrane glycoprotein. The protein catalyzes the final
            step in the conversion of angiotensinogen to angiotensin IV (AT4)
            and is also a receptor for AT4. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) contains an alternate 5' exon,
            which results in a distinct 5' UTR and use of a downstream start
            codon, compared to variant 1. The resulting isoform (2) has a
            shorter N-terminus when compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U62769.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC008850.7         88935-88953         c
            20-1482             U62769.1           11-1473
            1483-1483           AC008865.3         6956-6956
            1484-3619           U62768.1           1220-3355
            3620-3906           BC028692.1         3350-3636
            3907-3942           BQ923659.1         605-640
            3943-4281           BG622952.1         49-387
            4282-12383          AC008865.3         43327-51428
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q15"
     gene            1..12383
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="leucyl and cystinyl aminopeptidase"
                     /db_xref="GeneID:4012"
                     /db_xref="HGNC:HGNC:6656"
                     /db_xref="MIM:151300"
     exon            1..344
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            345..1185
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             368..3403
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /EC_number="3.4.11.3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     placental leucine aminopeptidase; angiotensin IV receptor;
                     AT (4) receptor; vasopressinase; insulin-responsive
                     aminopeptidase; insulin-regulated aminopeptidase;
                     leucyl-cystinyl aminopeptidase; oxytocinase; otase;
                     insulin-regulated membrane aminopeptidase"
                     /codon_start=1
                     /product="leucyl-cystinyl aminopeptidase isoform 2"
                     /protein_id="NP_787116.2"
                     /db_xref="CCDS:CCDS43346.1"
                     /db_xref="GeneID:4012"
                     /db_xref="HGNC:HGNC:6656"
                     /db_xref="MIM:151300"
                     /translation="MIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGL
                     GEHEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAF
                     VIVVAVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVP
                     LRYELSLHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISRVTFMSAVSSQEKQA
                     EILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFA
                     ATQFEPLAARSAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEF
                     SESVKMSTYLVAFIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQN
                     YFEIQYPLKKLDLVAIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAH
                     ELAHQWFGNLVTMKWWNDLWLNEGFATFMEYFSLEKIFKELSSYEDFLDARFKTMKKD
                     SLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVLYLHNHS
                     YASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLN
                     MKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINM
                     NGYYIVHYADDDWEALIHQLKINPYVLSDKDRANLINNIFELAGLGKVPLKRAFDLIN
                     YLGNENHTAPITEALFQTDLIYNLLEKLGYMDLASRLVTRVFKLLQNQIQQQTWTDEG
                     TPSMRELRSALLEFACTHNLGNCSTTAMKLFDDWMASNGTQSLPTDVMTTVFKVGAKT
                     DKGWSFLLGKYISIGSEAEKNKILEALASSEDVRKLYWLMKSSLNGDNFRTQKLSFII
                     RTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLFSTKTHLSEVQAF
                     FENQSEATFRLRCVQEALEVIQLNIQWMEKNLKSLTWWL"
     exon            1186..1324
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1456
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1577
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1732
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1846
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1978
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2110
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2271
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2360
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2544
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2701
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2886
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2887..3048
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..3189
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3271
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3272..12383
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12362..12367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="hexamer: AATAAA"
     polyA_site      12383
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="major polyA site"
ORIGIN      
        1 acagctgaac ttgggcaagc tcctaactcc actaagtaca gaagtggagc cgtacagcca
       61 tgaatgtcag gcatgttgtg gggactagtt gtattagttt tctattgcca ccataacaaa
      121 ttaccacaaa cttaagcaat atagtacaaa tttatcttaa agttattggc aggtcagaag
      181 cctgatacgg atctcattgg gctaaaacga aggagttggc agggctgtgt gttcctttct
      241 gtaggctctg ggcaggatcc atttcctgca cttttttggc ttttggaggt cgtccacatt
      301 ccttggctcc tggccctgct ccttcatctt caaagctacc aaagatcggc ttcagctccc
      361 caggaatatg attgaaaaca gcatgtttga ggaagaacca gatgtggtgg atttagccaa
      421 agagccttgt ttacatcctc tagagcctga tgaggtggaa tatgagcccc ggggttcccg
      481 actgctggtg cggggtcttg gtgagcatga gatggaggag gatgaagagg attatgagtc
      541 atcagcaaag ctgctgggca tgtccttcat gaatagaagc tcaggccttc ggaacagtgc
      601 aactggttac aggcagagcc cagatggggc ttgttcagta ccctctgcaa ggaccatggt
      661 ggtctgtgct tttgtcatcg tggttgctgt ttctgtaatc atggtgattt acttactgcc
      721 cagatgtacc tttaccaaag aaggctgcca taaaaaaaac cagtcaattg gactaattca
      781 gccatttgca acaaatggga aattgtttcc atgggcacag atcaggcttc ccactgccgt
      841 tgtgccacta cgctatgaac tcagcctaca cccgaaccta acctcgatga cattcagggg
      901 ttctgtgaca atttcagttc aggctcttca ggtcacatgg aatatcattc ttcatagcac
      961 aggtcataat atttcaagag tgacctttat gtcagcagtt tcaagccaag aaaaacaagc
     1021 tgagatcctg gaatatgcat atcatggaca gatcgccatt gttgcccccg aagcccttct
     1081 agcagggcac aattatacgt tgaagataga gtactcggca aatatatcta gttcttatta
     1141 tgggttttat ggcttctcct acacagatga aagtaatgag aaaaagtact ttgcagcaac
     1201 tcagtttgaa cccctggcag caagatctgc ttttccttgt tttgatgaac cagcatttaa
     1261 agccactttt atcatcaaga tcataaggga tgagcaatac accgctttat caaatatgcc
     1321 taagaagtca tcagtcgttc tagatgatgg acttgttcag gatgagtttt ctgagagtgt
     1381 gaagatgagc acttacttgg ttgctttcat tgtgggagag atgaagaacc tgagtcagga
     1441 cgtaaatgga accctggttt ctatatatgc tgtaccagaa aagattggtc aagttcatta
     1501 tgccttggaa acaactgtga agcttcttga gttttttcaa aactactttg aaattcagta
     1561 cccacttaag aaattggatt tggtggctat tcctgacttt gaagcaggag caatggaaaa
     1621 ttggggtttg ctcaccttcc gagaggagac acttctgtat gacagtaaca cttcttcaat
     1681 ggcggataga aagctggtga ctaaaatcat tgctcatgag ctggcccacc agtggtttgg
     1741 caatctggta acaatgaagt ggtggaatga cctatggcta aatgaaggtt ttgccacttt
     1801 catggagtat ttctctttgg aaaaaatatt caaagagctt tctagttatg aagatttctt
     1861 agatgctcga tttaaaacca tgaagaaaga ttccttaaat tcatctcatc caatatcatc
     1921 atctgttcag tcttcagaac aaattgaaga aatgtttgat tctctttcct attttaaggg
     1981 atcttctctc ttgttgatgt tgaaaactta ccttagtgaa gatgtgtttc aacatgctgt
     2041 tgtcctttac ctgcataatc acagctatgc atctattcaa agtgatgatc tgtgggatag
     2101 ttttaatgag gtcacaaacc aaacactaga tgtaaagaga atgatgaaaa cctggaccct
     2161 gcagaaagga tttcctttag tgactgttca aaagaaagga aaggaacttt ttatacaaca
     2221 agagagattc tttttaaata tgaagcctga aattcagcct tcagatacaa gctacctgtg
     2281 gcatattcca ctatcctatg tcactgaagg aagaaattat tcaaaatatc aatcggtatc
     2341 attactggat aagaaatcag gtgtcatcaa tcttacagaa gaagtgctgt gggtcaaagt
     2401 gaatataaac atgaatggtt attatattgt acactatgca gatgatgatt gggaagcact
     2461 aatccatcag ttgaaaataa atccttatgt tctgagtgac aaagaccgag ccaaccttat
     2521 caacaacatc tttgaacttg caggcctagg caaggtacct ctcaagaggg cctttgattt
     2581 gattaattat cttggaaatg agaaccatac tgcacccatc accgaagccc tgtttcagac
     2641 agacctcatc tataacctcc ttgaaaaact gggatacatg gatctggcct caagactggt
     2701 gactagggta tttaaattac ttcaaaacca aattcaacaa caaacttgga ctgatgaggg
     2761 cactccatct atgcgagagc ttcggtcagc cctgctagag tttgcttgca cccacaacct
     2821 ggggaactgc tctactactg ccatgaaact gtttgatgac tggatggcat ccaatggaac
     2881 tcaaagccta cctactgatg tcatgacaac tgtgttcaaa gttggagcaa aaactgacaa
     2941 aggctggtca ttccttttgg gcaaatacat ttctataggc tctgaagcag agaagaacaa
     3001 aatactagaa gcacttgcca gctcagagga tgtgcggaag ctttactggt taatgaaaag
     3061 tagcctgaat ggagataact tccgaacaca gaagctgtct tttatcatta gaacagtggg
     3121 tcgacatttt cctggacact tactggcatg ggattttgtc aaagagaact ggaataagct
     3181 tgtacagaag ttccctctgg ggtcctatac catacaaaat attgttgctg gatcaactta
     3241 cctgttttca acaaagacac atttatctga ggttcaggca ttctttgaaa atcagtcaga
     3301 ggcaaccttc cggcttcgtt gtgtccagga ggctttggaa gtcattcagt tgaatatcca
     3361 gtggatggag aagaacctca aaagtctcac atggtggctg tagcatgcac aaccgcacct
     3421 cattttgttg cccattcaga gagcttgtaa gcttgggctc tgccgctttt gcaaaagcca
     3481 aggtaaagcc aggatcgctg ccaagttgtt tgcactcttt ggagttctag ttagctcagg
     3541 gcctgactgt atttttcatc catcttttct gaagtgtctt tgggcagtat gtagttattt
     3601 attacaaaat tatattcacc taaatgccaa ccatctacaa aaacaatgag taatttttct
     3661 actttgaaga tacacagatg gggacaaaaa ccctgttttg gaattctgtt ctattcctca
     3721 gtatccagaa agttactgac acagtaaaac aaggaaagtt ctaccctaag agccgccatc
     3781 acttcaggcc gctggtttgt cagccatctg ttgcttctta ttgatagatg gcattggaat
     3841 gtggtacaaa gttagctctg aagaatatgg taacgaagac aataaagcat gcactgtaag
     3901 aactgacctc aggtgtgcag atctactttg atttggggtt ttgattaatt ctttattttt
     3961 ctgaaaaaaa gtttaaataa tgtctgtaac tattgtgttt ttttcctgcc gaatagccag
     4021 gtgctacaga tttttaaaat ttttgttgta ttgaaaagct aaacaaggcc aaaaggttaa
     4081 attttttgaa tatttaagac tttctttttc atcttttata gcgttaccat aggaaactgt
     4141 tccaaactga gttagcttta attataatta ttttattcat aaatggtgat agtccccaga
     4201 tctgtacacc tttatcactc cctgccgtag atatacttta ggttagtatt tctacattcg
     4261 tggcaagcat tttggtaaca ccagtggttt tcattgtgac ttttatcact atcactaaga
     4321 atgcaattta agttgccttt tggcatcata gatcatagaa actttctctc tgaatcatac
     4381 ccatgtgtga attttcattt tatatgatag tgtagcatat tagaagcata ttttaaaaca
     4441 gagcctttga gaaattgaaa aacaattatt tgaagttcac catagtacct taaaggaaca
     4501 aagagaatgt gggataggag taacttgaat tcgtgtttag tatggtgggc tttgaatttt
     4561 tttcactatc aaaaatagtg cttttgttta gttaaaaatt gtagtttttc tttttttgtg
     4621 tgtccaaact aacataaaag gagtggtcaa agaaggaaag aagaaaaatg agggaggatg
     4681 tccgaaactg tatggcattt tactccttct gaaagagaaa tactgtactt gaatttttga
     4741 gctatgcaga cttataccta gattgttgtg tttctttgat tcttaggaga aaagctttct
     4801 tcctaataat tcttgagaac tttatatttt gtttgaaaac agtttctatg tacataaaaa
     4861 gggtatttac ccatttgtga gcacttaaaa cactccacag agtcagacag tgggaaaggt
     4921 cacccttttt ttattcggca ggtatcaagt tagaatgtat ggaattgtga ataaaattat
     4981 attttatttt atgaaaatat ttttatataa taaaatttaa aaggctggta agctaaaaaa
     5041 atagtcttct ttaatcctag tgtgttttcc ccaatttagt cttttctaaa atttatctgt
     5101 tttagagtag aaaatattat atcctgagaa tcacagaatc atctaaatat gtcttattca
     5161 actgaagtga ttgtaagtct ttattcttgt gctttggtta aatgaatgat aatttagttg
     5221 atagtgaaag ttaatgtgtt tttatatttt tagagtgagg gaaaaatact aaacttttga
     5281 gagttttgtt gtcgttgctg gttttttacc atgtggtact aaattccact ttgatacaaa
     5341 atttaaattt tgtaacttct caaaatcact taagattctt cagttttacc tgtgcttctt
     5401 gacatcgctc ctataatgta tgttagttaa ctctgcatat ggcaaatctt gtcttgaatt
     5461 atatcatttc tctccctctc tctctctctc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5521 tgtgtgtgtg tgtgtgtgta gtgtgggggg tattgttatg agtaaaactt tatcaaaact
     5581 tggcctgaaa aagagataac tcatgatcca ttgctatctt tattactcaa aggctgttca
     5641 ttcccataat accagatcca taatagttgt agtttttttg atgttgttgc ccttacctta
     5701 attatttgta gttttagtag gccttgcctc agtttttccc acagaattcc ataattttaa
     5761 tgttgaacta acccatctat ataggacttc taaaaactac gtcaggaatg taaggttaaa
     5821 tgagaggtag aacttgtagt gtaataggta taatggtagc tttcaaagtt ttatcttctc
     5881 aaaacattgt cttagccagt gtatttctga gaactcagac tgaagcacag aagtcacagt
     5941 atgcttcttt tatttaagca cacagattca tgacacaagt acttgttgaa caagtgttct
     6001 cattgcagag agatctactt gaatataaaa gggtcatcat actagattat taaaaaagaa
     6061 ttggaaatct tgagttgtgg aagaaatgtt tatgctttgg agatctaaaa aagttatgaa
     6121 tgtaggaatt agataagtcc agtttgttct gttaatataa aatggttaga tatattaaac
     6181 tgcaagtagt atcagcttca ccataagctt gggccttcct ccatcttgtt ttatttaagt
     6241 ctctttaaat gaaattttgt tgtcattgtt tcagcattca tgaactttct tactatcaca
     6301 ggttctactt taattttcct tgccatatgt gtttccattt tgtgggacat tagccaccac
     6361 aggaacacaa gagttgtcag aagactcctg ggtgtacaga gcaaatcaag ctgcatcagt
     6421 attataggat accaaacaga atcctttcta ttaggaaata agaaatcagt taagtgttgg
     6481 ctgggcgcgg tggcttatgc ctgtaatccc agcattttgg gatgccaagg tgggcagacc
     6541 atttgaggcc aggagttcta aaccagtttg gccgacatgg caaaacccca tctctactaa
     6601 aaatataaaa attagctggg catggtggtg cacacctgta atcccagcta cctgggaggc
     6661 acgagaatca cttgaaccca ggaggcggag gttgcagtga gccaaggttg tgccactgca
     6721 ctccagcctg ggtgacaaag cgagactgtc tcaaaaaaca aaaaacaaaa aaaaaagagt
     6781 taagtgttga ttttgttgga ttactgacag tttgttgaga tggattttaa aaagagtcta
     6841 tttaaaccaa agtacctgga accataatct gccatcagat tgctaaacat tcacataatc
     6901 cccatgtttc atggggcttc agggaaggca aatacatctg agatgagcca cctccaagga
     6961 cagcatttcc tgggctgcaa aactagatgt ggattttgtt acttaggcct ctctaaaaac
     7021 tgaggccatg gatatgacat agcttttctt ctcacctatg cctttgttac ttttgtattt
     7081 attggaatta aatctctttt taaagagctt ccttccccct tcatcccatt tgtattttta
     7141 aaaactaagt tatatatata tttgcttgtt tttgcattct tttattttgt gctccattcc
     7201 ctgctttgcc agttaagtac tacctgaaaa gtagcggttc aaaagtatgg taaatctcct
     7261 cccttaacct tattgcctgt gtgggtgaca gctaatggaa aagttgatcg atttttttca
     7321 gtggtttttt tgggagtata tactttaaaa atgtacatac atgtttttga tagtttttat
     7381 attatttcat tttgtatatt tttttcctga tgaagagtga tatatcatcc tatgaaaatt
     7441 tcatctaccc ttcccatttt acctgtatgg gcagtagcta cagactagtt atttggtttc
     7501 cttctttgga agaaggtggt gatgttgaga ggagagcaag ctatattctt ttaaaagtgt
     7561 gtacagatgt ttttgctatt ttgtgttatt tcattttgta tttcattcat ccattttgtg
     7621 gacgtgtaga taaaacgcgg tggtccaaaa gcatgacaga tgtccccttt gcgtttctcc
     7681 ttattgttcg catggacaat aactgacaga gtagtcctag ttgctgttgt tttgatctag
     7741 tcttggggct ggggttgtat attattcttt caagtgtgta taaatattgt gccacctttt
     7801 tatacttcat tttatacttg tttccttgta tggatgcata tatacttaat gaaaattgag
     7861 gttcagagta tgacaaatct cttttccctt ttccttactg cctatttagg caatagctaa
     7921 tagagtagtt tgttttgttt tggggtgtgg gggagagtgg agtatgtact taatgtatat
     7981 aaataaactt gctactacat tgtattacta atttggttca cactgtgtcc ttctcatgga
     8041 tgtgaaagta ccaatgaaat gtggagatct cgatgtataa cagtctccca ttctcctcat
     8101 cccttattga ctgctgggca gtagctaaca gagtagttgt caagtggcgt atttgaggtg
     8161 agggggaggg acggggaagg taggaaaatg cacttaatgt atatacatgt ttttactact
     8221 acattgtatt aattcatcgg gtgcccagac tgaaaggtac ctaatgaacc atggagttcc
     8281 ggatgtataa cagtctcctc ttttcccttt cccttattgc ccatgtgggc aatacttaag
     8341 agtagtttgg gtttattgaa gattttttgc taggagagag aaaatttttt gctaggagag
     8401 gtttcaaggt aagagtatat actttaaaca tgtatataaa tgtttttgct acttttctgt
     8461 cactaccttt cttaccttgt cctttacatg gatatatgaa gaaaattgag gttcagcctg
     8521 tagcagctct cctcttctgc ccttccctta gattgtctgt gtgggcaggt gtctaagaga
     8581 gtagttgatt agcttcaggg attctttggt gggtgttggg gtatatactt ttgtatatgt
     8641 atgtacatat ttttatttct ttattatagt gtttcattgg gtacccggtc cttcacttgg
     8701 atgtgtaggt tcctaatgag ccatggagtt atggatgtat aacaatctgt tctcccttta
     8761 cccttttaat gtatatgggc aataatttta aagtagtttg gattttttta agttttgagg
     8821 ggtgggaggg gtctaaggca gaatatatcc tttttaaaaa tgtatatata cttttcatta
     8881 actatattgt attatttcat gctgtaccca gtccattgct tggacttacg ggtacctaat
     8941 gaaacgtgga ggtccggatg tatgaaaatc tcctcttttc ccctttcctt attgcctctg
     9001 taggcaataa ttatagagta gtatagatgg tttttctttt ttatccatct gcctatctcc
     9061 aggaggatgg ggtgtgcact tttagaaatg tatataaatg ttcttgctcc tttttcctgt
     9121 tacttcattg tgtgccaagg cctttccatg gatacttagg tacgtaatga aaattgaggt
     9181 tcattctatg aaaaaatctc cccttctccc cttcccttat tgcctgtctt ggcaatggcc
     9241 agtagagtag ttatttaagg gattttgttt ttttgttttt ttgttttttt tttggtaggg
     9301 gaggtgggga atttctgggt ttgtcttttt tttaatgtat ataaaagttt gctagttgtt
     9361 gttattgttg ttctttcact ctcaacacag ttttttgttt ggagttgtag gaatcaaatg
     9421 taatgaggga cccagacccg agagtgtggt aaatattctt tgccttcctc agagcctgta
     9481 tacacaagca agtagtatgt gagcactaca gggcttgggt tttggttttt tgcttttgag
     9541 ggttttaatg aggagtggga ttgttgagag gaaaaggcaa gatatataat ttttttaatg
     9601 tatataaatg ttttgctcct ttttgatgtt aacttcattt tgaatctggt cctttgtatg
     9661 accacttagg tacttaatag taataaagtt gagattgact ctatgaaaaa tatcccatac
     9721 tccccatccc tcattgccta gggggcaatg gtagaataga tgtttggttt cttttggggg
     9781 tgtctgagtg tatatgtgtg tgtatataca cacaagaaca cacatattta tatactatac
     9841 acacacattt taatgtatat aaatatttgc tacattctgt gtgttatata atgtggtacc
     9901 cagtcctctg ctgggacatg gatgtacata atgaaacatg gaggtccaga cgtatgataa
     9961 ctctcctgtt tcccttccct cattgcctac aggggcaata gtttcatatc ttgggttttt
    10021 tattgtttaa ttttttttta tggggagggg ttctttgggt gggtaatagt cagggggaaa
    10081 ggacagtgtc tatacttttt aaagatgtat ataaatgttt catgttattg gttttgtacc
    10141 tagtcctttg catggatata taggtaccta atgaaaatcg aggatcagtg tatgacaaat
    10201 ctcccatcct cccctttcct tattgcctgt gtcggcaata ggaagtagaa tagttgtgtg
    10261 ttgtttactt acttgtctgt tttagagaga tttctatttt tggtagggga atattcttaa
    10321 tatgttttca tatctttatt tcattttgta gtcttttgca tggctatgta gggacctaat
    10381 gaaagtcgag tttcataata tgacagctca cttcttttcc tacatatttc ctcacttagc
    10441 agtagctagt gtagttattt tgtggttatt ttatttcatt ctctaggatc tattccatta
    10501 aggatgcaag tgtgtagatg catatatatc atttgcttct ttcttccgcc attattttcc
    10561 tttaacactt agtcccttgc aaggggtctg actataccta acaaaaataa agacctacta
    10621 aatccctgcc atctggctgg aaggtgtagc agtgtattaa tgtaactaat agcaacaggt
    10681 gtgcagcaga taagacagga gaagacactc agctatggtt gagttgtgaa agctaattag
    10741 atccagcatt tcttatgttg attcagattg aatgaggtag tgaaaaggga cctgtgcttt
    10801 tgtaaattct agaaaggcaa gaggacaata ttgaataaag ctgtgtttaa acttcattgc
    10861 tcatatatct tttagtaact ggtaaaccgg cgaagctgat aagccaataa gagtgctgtc
    10921 tagaacgcat ttgcaaaccg ttaagagtag cttctttttg aggcctggac ttctgattca
    10981 tctatagaag tctattatga ttccatcaat tgcatagctt gtgtacctgg tagaaattgt
    11041 gtcttggaat gaccctttcg agttattgac atggctctga tgaatagaac atgagcccca
    11101 aaactaaatc caaaaggaat tttctatctt tcatccccca catgtggcaa gacaagttgg
    11161 ccctttctta cccagaggtc ttttgtgtga ctgcatcttt ctcctccgtt ctccattgtg
    11221 tgctttccat tttgtcttta gtgcctatac tgttaggtgt tttcttcact ggcattcaca
    11281 aatttaagcc attgctgcct cattagcctt gtattttgtg tgcatatcat gtatccagac
    11341 ctgtatgttc gctttaagca ttcttatatc acactgtctc ctcatctacc atatggtaaa
    11401 tgttaaaact ccacatttgt ctgcatcagg gaaaatgcat gggcacacat cctccctccc
    11461 tccctctctg ctctcctccc ttccttcagg cctcttagca ttgtttgttt tcccatttct
    11521 gatactacta ctccatgctg aagatttgcc atattactat tttggaaaca ttgagtgata
    11581 gaactcctag aaaatttgca aagaaatgtt acatactgta tatcaaactc tcagattcta
    11641 gtgttgaaaa agtagcctat actttgctat tacttatacc tgctgccata gaaaaaaata
    11701 agtttattca tgacacattt acatttgatc ataaataaaa gaaaaaaggg cacctttttg
    11761 gagttagtca tggtagtcat tagtgatatt tctgaacagt tcttaattta aaatacttca
    11821 aaggaagtaa aggtcatggc ttagctgaag gaaatgctcc agaaattgga ctgtgtaaac
    11881 catcagtaca ataatacgct gtgtatgtat gtgtatataa aatgagaatt atggcataat
    11941 tggagcattt gcattaatca acaaactcac attgagacaa aacttagttt tacagctgtc
    12001 ttgattaaag ccaagtgttc catgttgctg tgaagaatag cctctttcaa atactttgga
    12061 aagtagttac ttggaaactt gtaaaggtat tacattttta tatttaaaca cctatagaga
    12121 tcttcaattc cttgagtctg agcttgtggg tggaattcta aatttgtatc ataatctgtc
    12181 ttttgtgaaa cattttgaaa atatgtatat ataatattgt atatgcaaat tgtgttgttt
    12241 cacttgtaaa gggaaaaggc ttatttttct ttatatttct gataacttgt tttgcatatg
    12301 accagcactg actgaaaggc atgtgtagct gcaaacactg ttgctttttt tgtgaaatga
    12361 aaataaaagt atttaaatac aaa
//
