LOCUS       NM_005575              12134 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens leucyl and cystinyl aminopeptidase (LNPEP), transcript
            variant 1, mRNA.
ACCESSION   NM_005575
VERSION     NM_005575.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12134)
  AUTHORS   Marusina,A.I., Ji-Xu,A., Le,S.T., Toussi,A., Tsoi,L.C., Li,Q.,
            Luxardi,G., Nava,J., Downing,L., Leal,A.R., Kuzminykh,N.Y.,
            Kruglinskaya,O., Bruggen,M.C., Adamopoulos,I.E., Merleev,A.A.,
            Gudjonsson,J.E. and Maverakis,E.
  TITLE     Cell-Specific and Variant-Linked Alterations in Expression of
            ERAP1, ERAP2, and LNPEP Aminopeptidases in Psoriasis
  JOURNAL   J Invest Dermatol 143 (7), 1157-1167 (2023)
   PUBMED   36716917
  REMARK    GeneRIF: Cell-Specific and Variant-Linked Alterations in Expression
            of ERAP1, ERAP2, and LNPEP Aminopeptidases in Psoriasis.
REFERENCE   2  (bases 1 to 12134)
  AUTHORS   Khaliq,O.P., Konoshita,T., Moodley,J. and Naicker,T.
  TITLE     The role of LNPEP and ANPEP gene polymorphisms in the pathogenesis
            of pre-eclampsia
  JOURNAL   Eur J Obstet Gynecol Reprod Biol 252, 160-165 (2020)
   PUBMED   32619880
  REMARK    GeneRIF: The role of LNPEP and ANPEP gene polymorphisms in the
            pathogenesis of pre-eclampsia.
REFERENCE   3  (bases 1 to 12134)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 12134)
  AUTHORS   Liu,W.H., Fang,Y.N., Wu,C.C., Chen,M.C., Chang,J.P., Lin,Y.S.,
            Pan,K.L., Ho,W.C., Chang,T.H., Huang,Y.K., Fang,C.Y., Chen,C.J. and
            Lee,W.C.
  TITLE     Differential Gene Expression Profile of Renin-Angiotensin System in
            the Left Atrium in Mitral Regurgitation Patients
  JOURNAL   Dis Markers 2018, 6924608 (2018)
   PUBMED   30581499
  REMARK    GeneRIF: LNPEP expression in the left atrium in mitral
            regurgitation patients significantly differed from those in aortic
            valve disease patients and normal controls.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12134)
  AUTHORS   Mpakali,A., Saridakis,E., Harlos,K., Zhao,Y., Kokkala,P.,
            Georgiadis,D., Giastas,P., Papakyriakou,A. and Stratikos,E.
  TITLE     Ligand-Induced Conformational Change of Insulin-Regulated
            Aminopeptidase: Insights on Catalytic Mechanism and Active Site
            Plasticity
  JOURNAL   J Med Chem 60 (7), 2963-2972 (2017)
   PUBMED   28328206
  REMARK    GeneRIF: This study describes a crystal structure of
            insulin-regulated aminopeptidase in complex with a recently
            developed bioactive and selective inhibitor at 2.53 A resolution.
REFERENCE   6  (bases 1 to 12134)
  AUTHORS   Itoh,C., Watanabe,M., Nagamatsu,A., Soeda,S., Kawarabayashi,T. and
            Shimeno,H.
  TITLE     Two molecular species of oxytocinase (L-cystine aminopeptidase) in
            human placenta: purification and characterization
  JOURNAL   Biol Pharm Bull 20 (1), 20-24 (1997)
   PUBMED   9013800
REFERENCE   7  (bases 1 to 12134)
  AUTHORS   Rogi,T., Tsujimoto,M., Nakazato,H., Mizutani,S. and Tomoda,Y.
  TITLE     Human placental leucine aminopeptidase/oxytocinase. A new member of
            type II membrane-spanning zinc metallopeptidase family
  JOURNAL   J Biol Chem 271 (1), 56-61 (1996)
   PUBMED   8550619
REFERENCE   8  (bases 1 to 12134)
  AUTHORS   Tsujimoto,M., Mizutani,S., Adachi,H., Kimura,M., Nakazato,H. and
            Tomoda,Y.
  TITLE     Identification of human placental leucine aminopeptidase as
            oxytocinase
  JOURNAL   Arch Biochem Biophys 292 (2), 388-392 (1992)
   PUBMED   1731608
REFERENCE   9  (bases 1 to 12134)
  AUTHORS   Mizutani,S., Akiyama,H., Kurauchi,O., Taira,H., Narita,O. and
            Tomoda,Y.
  TITLE     In vitro degradation of angiotensin II (A-II) by human placental
            subcellular fractions, pregnancy sera and purified placental
            aminopeptidases
  JOURNAL   Acta Endocrinol (Copenh) 110 (1), 135-139 (1985)
   PUBMED   3898693
REFERENCE   10 (bases 1 to 12134)
  AUTHORS   Beckman,L., Bjorling,G. and Christodoulou,C.
  TITLE     Pregnancy enzymes and placental polymorphism. II. Leucine
            aminopeptidase
  JOURNAL   Acta Genet Stat Med 16 (2), 122-131 (1966)
   PUBMED   5953194
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008850.7, U62768.1,
            AC008865.3, BC028692.1, BQ923659.1 and BG622952.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005575.2.
            
            Summary: This gene encodes a zinc-dependent aminopeptidase that
            cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin,
            dynorphin A and other peptide hormones. The protein can be secreted
            in maternal serum, reside in intracellular vesicles with the
            insulin-responsive glucose transporter GLUT4, or form a type II
            integral membrane glycoprotein. The protein catalyzes the final
            step in the conversion of angiotensinogen to angiotensin IV (AT4)
            and is also a receptor for AT4. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.179072.1,
                                           SRR1803614.114069.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000231368.10/ ENSP00000231368.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                AC008850.7         111239-111306       c
            69-1233             U62768.1           54-1218
            1234-1234           AC008865.3         6956-6956
            1235-3370           U62768.1           1220-3355
            3371-3657           BC028692.1         3350-3636
            3658-3693           BQ923659.1         605-640
            3694-4032           BG622952.1         49-387
            4033-12134          AC008865.3         43327-51428
FEATURES             Location/Qualifiers
     source          1..12134
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q15"
     gene            1..12134
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="leucyl and cystinyl aminopeptidase"
                     /db_xref="GeneID:4012"
                     /db_xref="HGNC:HGNC:6656"
                     /db_xref="MIM:151300"
     exon            1..95
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..3154
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /EC_number="3.4.11.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     placental leucine aminopeptidase; angiotensin IV receptor;
                     AT (4) receptor; vasopressinase; insulin-responsive
                     aminopeptidase; insulin-regulated aminopeptidase;
                     leucyl-cystinyl aminopeptidase; oxytocinase; otase;
                     insulin-regulated membrane aminopeptidase"
                     /codon_start=1
                     /product="leucyl-cystinyl aminopeptidase isoform 1"
                     /protein_id="NP_005566.2"
                     /db_xref="CCDS:CCDS4087.1"
                     /db_xref="GeneID:4012"
                     /db_xref="HGNC:HGNC:6656"
                     /db_xref="MIM:151300"
                     /translation="MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEV
                     EYEPRGSRLLVRGLGEHEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGA
                     CSVPSARTMVVCAFVIVVAVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKL
                     FPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISR
                     VTFMSAVSSQEKQAEILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYG
                     FSYTDESNEKKYFAATQFEPLAARSAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKK
                     SSVVLDDGLVQDEFSESVKMSTYLVAFIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHY
                     ALETTVKLLEFFQNYFEIQYPLKKLDLVAIPDFEAGAMENWGLLTFREETLLYDSNTS
                     SMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFATFMEYFSLEKIFKELSSY
                     EDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLKTYLSED
                     VFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQKGFPLVTVQKK
                     GKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVIN
                     LTEEVLWVKVNINMNGYYIVHYADDDWEALIHQLKINPYVLSDKDRANLINNIFELAG
                     LGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLEKLGYMDLASRLVTRVFKL
                     LQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAMKLFDDWMASNGTQSLP
                     TDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASSEDVRKLYWLMKSSL
                     NGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTY
                     LFSTKTHLSEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKSLTWWL"
     exon            96..936
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1075
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1207
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1328
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1483
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1597
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1729
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1861
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..2022
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2111
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2295
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2452
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2637
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2638..2799
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2800..2940
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3022
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..12134
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12113..12118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="hexamer: AATAAA"
     polyA_site      12134
                     /gene="LNPEP"
                     /gene_synonym="CAP; IRAP; P-LAP; PLAP"
                     /note="major polyA site"
ORIGIN      
        1 agtcagctgg gccgcctcag ctctcggagt aggaagctcg ggcgctccgg ctgtaaggag
       61 ccgcggcggg gggaaaatgg agcccttcac caatgatcgg cttcagctcc ccaggaatat
      121 gattgaaaac agcatgtttg aggaagaacc agatgtggtg gatttagcca aagagccttg
      181 tttacatcct ctagagcctg atgaggtgga atatgagccc cggggttccc gactgctggt
      241 gcggggtctt ggtgagcatg agatggagga ggatgaagag gattatgagt catcagcaaa
      301 gctgctgggc atgtccttca tgaatagaag ctcaggcctt cggaacagtg caactggtta
      361 caggcagagc ccagatgggg cttgttcagt accctctgca aggaccatgg tggtctgtgc
      421 ttttgtcatc gtggttgctg tttctgtaat catggtgatt tacttactgc ccagatgtac
      481 ctttaccaaa gaaggctgcc ataaaaaaaa ccagtcaatt ggactaattc agccatttgc
      541 aacaaatggg aaattgtttc catgggcaca gatcaggctt cccactgccg ttgtgccact
      601 acgctatgaa ctcagcctac acccgaacct aacctcgatg acattcaggg gttctgtgac
      661 aatttcagtt caggctcttc aggtcacatg gaatatcatt cttcatagca caggtcataa
      721 tatttcaaga gtgaccttta tgtcagcagt ttcaagccaa gaaaaacaag ctgagatcct
      781 ggaatatgca tatcatggac agatcgccat tgttgccccc gaagcccttc tagcagggca
      841 caattatacg ttgaagatag agtactcggc aaatatatct agttcttatt atgggtttta
      901 tggcttctcc tacacagatg aaagtaatga gaaaaagtac tttgcagcaa ctcagtttga
      961 acccctggca gcaagatctg cttttccttg ttttgatgaa ccagcattta aagccacttt
     1021 tatcatcaag atcataaggg atgagcaata caccgcttta tcaaatatgc ctaagaagtc
     1081 atcagtcgtt ctagatgatg gacttgttca ggatgagttt tctgagagtg tgaagatgag
     1141 cacttacttg gttgctttca ttgtgggaga gatgaagaac ctgagtcagg acgtaaatgg
     1201 aaccctggtt tctatatatg ctgtaccaga aaagattggt caagttcatt atgccttgga
     1261 aacaactgtg aagcttcttg agttttttca aaactacttt gaaattcagt acccacttaa
     1321 gaaattggat ttggtggcta ttcctgactt tgaagcagga gcaatggaaa attggggttt
     1381 gctcaccttc cgagaggaga cacttctgta tgacagtaac acttcttcaa tggcggatag
     1441 aaagctggtg actaaaatca ttgctcatga gctggcccac cagtggtttg gcaatctggt
     1501 aacaatgaag tggtggaatg acctatggct aaatgaaggt tttgccactt tcatggagta
     1561 tttctctttg gaaaaaatat tcaaagagct ttctagttat gaagatttct tagatgctcg
     1621 atttaaaacc atgaagaaag attccttaaa ttcatctcat ccaatatcat catctgttca
     1681 gtcttcagaa caaattgaag aaatgtttga ttctctttcc tattttaagg gatcttctct
     1741 cttgttgatg ttgaaaactt accttagtga agatgtgttt caacatgctg ttgtccttta
     1801 cctgcataat cacagctatg catctattca aagtgatgat ctgtgggata gttttaatga
     1861 ggtcacaaac caaacactag atgtaaagag aatgatgaaa acctggaccc tgcagaaagg
     1921 atttccttta gtgactgttc aaaagaaagg aaaggaactt tttatacaac aagagagatt
     1981 ctttttaaat atgaagcctg aaattcagcc ttcagataca agctacctgt ggcatattcc
     2041 actatcctat gtcactgaag gaagaaatta ttcaaaatat caatcggtat cattactgga
     2101 taagaaatca ggtgtcatca atcttacaga agaagtgctg tgggtcaaag tgaatataaa
     2161 catgaatggt tattatattg tacactatgc agatgatgat tgggaagcac taatccatca
     2221 gttgaaaata aatccttatg ttctgagtga caaagaccga gccaacctta tcaacaacat
     2281 ctttgaactt gcaggcctag gcaaggtacc tctcaagagg gcctttgatt tgattaatta
     2341 tcttggaaat gagaaccata ctgcacccat caccgaagcc ctgtttcaga cagacctcat
     2401 ctataacctc cttgaaaaac tgggatacat ggatctggcc tcaagactgg tgactagggt
     2461 atttaaatta cttcaaaacc aaattcaaca acaaacttgg actgatgagg gcactccatc
     2521 tatgcgagag cttcggtcag ccctgctaga gtttgcttgc acccacaacc tggggaactg
     2581 ctctactact gccatgaaac tgtttgatga ctggatggca tccaatggaa ctcaaagcct
     2641 acctactgat gtcatgacaa ctgtgttcaa agttggagca aaaactgaca aaggctggtc
     2701 attccttttg ggcaaataca tttctatagg ctctgaagca gagaagaaca aaatactaga
     2761 agcacttgcc agctcagagg atgtgcggaa gctttactgg ttaatgaaaa gtagcctgaa
     2821 tggagataac ttccgaacac agaagctgtc ttttatcatt agaacagtgg gtcgacattt
     2881 tcctggacac ttactggcat gggattttgt caaagagaac tggaataagc ttgtacagaa
     2941 gttccctctg gggtcctata ccatacaaaa tattgttgct ggatcaactt acctgttttc
     3001 aacaaagaca catttatctg aggttcaggc attctttgaa aatcagtcag aggcaacctt
     3061 ccggcttcgt tgtgtccagg aggctttgga agtcattcag ttgaatatcc agtggatgga
     3121 gaagaacctc aaaagtctca catggtggct gtagcatgca caaccgcacc tcattttgtt
     3181 gcccattcag agagcttgta agcttgggct ctgccgcttt tgcaaaagcc aaggtaaagc
     3241 caggatcgct gccaagttgt ttgcactctt tggagttcta gttagctcag ggcctgactg
     3301 tatttttcat ccatcttttc tgaagtgtct ttgggcagta tgtagttatt tattacaaaa
     3361 ttatattcac ctaaatgcca accatctaca aaaacaatga gtaatttttc tactttgaag
     3421 atacacagat ggggacaaaa accctgtttt ggaattctgt tctattcctc agtatccaga
     3481 aagttactga cacagtaaaa caaggaaagt tctaccctaa gagccgccat cacttcaggc
     3541 cgctggtttg tcagccatct gttgcttctt attgatagat ggcattggaa tgtggtacaa
     3601 agttagctct gaagaatatg gtaacgaaga caataaagca tgcactgtaa gaactgacct
     3661 caggtgtgca gatctacttt gatttggggt tttgattaat tctttatttt tctgaaaaaa
     3721 agtttaaata atgtctgtaa ctattgtgtt tttttcctgc cgaatagcca ggtgctacag
     3781 atttttaaaa tttttgttgt attgaaaagc taaacaaggc caaaaggtta aattttttga
     3841 atatttaaga ctttcttttt catcttttat agcgttacca taggaaactg ttccaaactg
     3901 agttagcttt aattataatt attttattca taaatggtga tagtccccag atctgtacac
     3961 ctttatcact ccctgccgta gatatacttt aggttagtat ttctacattc gtggcaagca
     4021 ttttggtaac accagtggtt ttcattgtga cttttatcac tatcactaag aatgcaattt
     4081 aagttgcctt ttggcatcat agatcataga aactttctct ctgaatcata cccatgtgtg
     4141 aattttcatt ttatatgata gtgtagcata ttagaagcat attttaaaac agagcctttg
     4201 agaaattgaa aaacaattat ttgaagttca ccatagtacc ttaaaggaac aaagagaatg
     4261 tgggatagga gtaacttgaa ttcgtgttta gtatggtggg ctttgaattt ttttcactat
     4321 caaaaatagt gcttttgttt agttaaaaat tgtagttttt ctttttttgt gtgtccaaac
     4381 taacataaaa ggagtggtca aagaaggaaa gaagaaaaat gagggaggat gtccgaaact
     4441 gtatggcatt ttactccttc tgaaagagaa atactgtact tgaatttttg agctatgcag
     4501 acttatacct agattgttgt gtttctttga ttcttaggag aaaagctttc ttcctaataa
     4561 ttcttgagaa ctttatattt tgtttgaaaa cagtttctat gtacataaaa agggtattta
     4621 cccatttgtg agcacttaaa acactccaca gagtcagaca gtgggaaagg tcaccctttt
     4681 tttattcggc aggtatcaag ttagaatgta tggaattgtg aataaaatta tattttattt
     4741 tatgaaaata tttttatata ataaaattta aaaggctggt aagctaaaaa aatagtcttc
     4801 tttaatccta gtgtgttttc cccaatttag tcttttctaa aatttatctg ttttagagta
     4861 gaaaatatta tatcctgaga atcacagaat catctaaata tgtcttattc aactgaagtg
     4921 attgtaagtc tttattcttg tgctttggtt aaatgaatga taatttagtt gatagtgaaa
     4981 gttaatgtgt ttttatattt ttagagtgag ggaaaaatac taaacttttg agagttttgt
     5041 tgtcgttgct ggttttttac catgtggtac taaattccac tttgatacaa aatttaaatt
     5101 ttgtaacttc tcaaaatcac ttaagattct tcagttttac ctgtgcttct tgacatcgct
     5161 cctataatgt atgttagtta actctgcata tggcaaatct tgtcttgaat tatatcattt
     5221 ctctccctct ctctctctct ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     5281 gtgtgtgtgt agtgtggggg gtattgttat gagtaaaact ttatcaaaac ttggcctgaa
     5341 aaagagataa ctcatgatcc attgctatct ttattactca aaggctgttc attcccataa
     5401 taccagatcc ataatagttg tagttttttt gatgttgttg cccttacctt aattatttgt
     5461 agttttagta ggccttgcct cagtttttcc cacagaattc cataatttta atgttgaact
     5521 aacccatcta tataggactt ctaaaaacta cgtcaggaat gtaaggttaa atgagaggta
     5581 gaacttgtag tgtaataggt ataatggtag ctttcaaagt tttatcttct caaaacattg
     5641 tcttagccag tgtatttctg agaactcaga ctgaagcaca gaagtcacag tatgcttctt
     5701 ttatttaagc acacagattc atgacacaag tacttgttga acaagtgttc tcattgcaga
     5761 gagatctact tgaatataaa agggtcatca tactagatta ttaaaaaaga attggaaatc
     5821 ttgagttgtg gaagaaatgt ttatgctttg gagatctaaa aaagttatga atgtaggaat
     5881 tagataagtc cagtttgttc tgttaatata aaatggttag atatattaaa ctgcaagtag
     5941 tatcagcttc accataagct tgggccttcc tccatcttgt tttatttaag tctctttaaa
     6001 tgaaattttg ttgtcattgt ttcagcattc atgaactttc ttactatcac aggttctact
     6061 ttaattttcc ttgccatatg tgtttccatt ttgtgggaca ttagccacca caggaacaca
     6121 agagttgtca gaagactcct gggtgtacag agcaaatcaa gctgcatcag tattatagga
     6181 taccaaacag aatcctttct attaggaaat aagaaatcag ttaagtgttg gctgggcgcg
     6241 gtggcttatg cctgtaatcc cagcattttg ggatgccaag gtgggcagac catttgaggc
     6301 caggagttct aaaccagttt ggccgacatg gcaaaacccc atctctacta aaaatataaa
     6361 aattagctgg gcatggtggt gcacacctgt aatcccagct acctgggagg cacgagaatc
     6421 acttgaaccc aggaggcgga ggttgcagtg agccaaggtt gtgccactgc actccagcct
     6481 gggtgacaaa gcgagactgt ctcaaaaaac aaaaaacaaa aaaaaaagag ttaagtgttg
     6541 attttgttgg attactgaca gtttgttgag atggatttta aaaagagtct atttaaacca
     6601 aagtacctgg aaccataatc tgccatcaga ttgctaaaca ttcacataat ccccatgttt
     6661 catggggctt cagggaaggc aaatacatct gagatgagcc acctccaagg acagcatttc
     6721 ctgggctgca aaactagatg tggattttgt tacttaggcc tctctaaaaa ctgaggccat
     6781 ggatatgaca tagcttttct tctcacctat gcctttgtta cttttgtatt tattggaatt
     6841 aaatctcttt ttaaagagct tccttccccc ttcatcccat ttgtattttt aaaaactaag
     6901 ttatatatat atttgcttgt ttttgcattc ttttattttg tgctccattc cctgctttgc
     6961 cagttaagta ctacctgaaa agtagcggtt caaaagtatg gtaaatctcc tcccttaacc
     7021 ttattgcctg tgtgggtgac agctaatgga aaagttgatc gatttttttc agtggttttt
     7081 ttgggagtat atactttaaa aatgtacata catgtttttg atagttttta tattatttca
     7141 ttttgtatat ttttttcctg atgaagagtg atatatcatc ctatgaaaat ttcatctacc
     7201 cttcccattt tacctgtatg ggcagtagct acagactagt tatttggttt ccttctttgg
     7261 aagaaggtgg tgatgttgag aggagagcaa gctatattct tttaaaagtg tgtacagatg
     7321 tttttgctat tttgtgttat ttcattttgt atttcattca tccattttgt ggacgtgtag
     7381 ataaaacgcg gtggtccaaa agcatgacag atgtcccctt tgcgtttctc cttattgttc
     7441 gcatggacaa taactgacag agtagtccta gttgctgttg ttttgatcta gtcttggggc
     7501 tggggttgta tattattctt tcaagtgtgt ataaatattg tgccaccttt ttatacttca
     7561 ttttatactt gtttccttgt atggatgcat atatacttaa tgaaaattga ggttcagagt
     7621 atgacaaatc tcttttccct tttccttact gcctatttag gcaatagcta atagagtagt
     7681 ttgttttgtt ttggggtgtg ggggagagtg gagtatgtac ttaatgtata taaataaact
     7741 tgctactaca ttgtattact aatttggttc acactgtgtc cttctcatgg atgtgaaagt
     7801 accaatgaaa tgtggagatc tcgatgtata acagtctccc attctcctca tcccttattg
     7861 actgctgggc agtagctaac agagtagttg tcaagtggcg tatttgaggt gagggggagg
     7921 gacggggaag gtaggaaaat gcacttaatg tatatacatg tttttactac tacattgtat
     7981 taattcatcg ggtgcccaga ctgaaaggta cctaatgaac catggagttc cggatgtata
     8041 acagtctcct cttttccctt tcccttattg cccatgtggg caatacttaa gagtagtttg
     8101 ggtttattga agattttttg ctaggagaga gaaaattttt tgctaggaga ggtttcaagg
     8161 taagagtata tactttaaac atgtatataa atgtttttgc tacttttctg tcactacctt
     8221 tcttaccttg tcctttacat ggatatatga agaaaattga ggttcagcct gtagcagctc
     8281 tcctcttctg cccttccctt agattgtctg tgtgggcagg tgtctaagag agtagttgat
     8341 tagcttcagg gattctttgg tgggtgttgg ggtatatact tttgtatatg tatgtacata
     8401 tttttatttc tttattatag tgtttcattg ggtacccggt ccttcacttg gatgtgtagg
     8461 ttcctaatga gccatggagt tatggatgta taacaatctg ttctcccttt acccttttaa
     8521 tgtatatggg caataatttt aaagtagttt ggattttttt aagttttgag gggtgggagg
     8581 ggtctaaggc agaatatatc ctttttaaaa atgtatatat acttttcatt aactatattg
     8641 tattatttca tgctgtaccc agtccattgc ttggacttac gggtacctaa tgaaacgtgg
     8701 aggtccggat gtatgaaaat ctcctctttt cccctttcct tattgcctct gtaggcaata
     8761 attatagagt agtatagatg gtttttcttt tttatccatc tgcctatctc caggaggatg
     8821 gggtgtgcac ttttagaaat gtatataaat gttcttgctc ctttttcctg ttacttcatt
     8881 gtgtgccaag gcctttccat ggatacttag gtacgtaatg aaaattgagg ttcattctat
     8941 gaaaaaatct ccccttctcc ccttccctta ttgcctgtct tggcaatggc cagtagagta
     9001 gttatttaag ggattttgtt tttttgtttt tttgtttttt ttttggtagg ggaggtgggg
     9061 aatttctggg tttgtctttt ttttaatgta tataaaagtt tgctagttgt tgttattgtt
     9121 gttctttcac tctcaacaca gttttttgtt tggagttgta ggaatcaaat gtaatgaggg
     9181 acccagaccc gagagtgtgg taaatattct ttgccttcct cagagcctgt atacacaagc
     9241 aagtagtatg tgagcactac agggcttggg ttttggtttt ttgcttttga gggttttaat
     9301 gaggagtggg attgttgaga ggaaaaggca agatatataa tttttttaat gtatataaat
     9361 gttttgctcc tttttgatgt taacttcatt ttgaatctgg tcctttgtat gaccacttag
     9421 gtacttaata gtaataaagt tgagattgac tctatgaaaa atatcccata ctccccatcc
     9481 ctcattgcct agggggcaat ggtagaatag atgtttggtt tcttttgggg gtgtctgagt
     9541 gtatatgtgt gtgtatatac acacaagaac acacatattt atatactata cacacacatt
     9601 ttaatgtata taaatatttg ctacattctg tgtgttatat aatgtggtac ccagtcctct
     9661 gctgggacat ggatgtacat aatgaaacat ggaggtccag acgtatgata actctcctgt
     9721 ttcccttccc tcattgccta caggggcaat agtttcatat cttgggtttt ttattgttta
     9781 attttttttt atggggaggg gttctttggg tgggtaatag tcagggggaa aggacagtgt
     9841 ctatactttt taaagatgta tataaatgtt tcatgttatt ggttttgtac ctagtccttt
     9901 gcatggatat ataggtacct aatgaaaatc gaggatcagt gtatgacaaa tctcccatcc
     9961 tcccctttcc ttattgcctg tgtcggcaat aggaagtaga atagttgtgt gttgtttact
    10021 tacttgtctg ttttagagag atttctattt ttggtagggg aatattctta atatgttttc
    10081 atatctttat ttcattttgt agtcttttgc atggctatgt agggacctaa tgaaagtcga
    10141 gtttcataat atgacagctc acttcttttc ctacatattt cctcacttag cagtagctag
    10201 tgtagttatt ttgtggttat tttatttcat tctctaggat ctattccatt aaggatgcaa
    10261 gtgtgtagat gcatatatat catttgcttc tttcttccgc cattattttc ctttaacact
    10321 tagtcccttg caaggggtct gactatacct aacaaaaata aagacctact aaatccctgc
    10381 catctggctg gaaggtgtag cagtgtatta atgtaactaa tagcaacagg tgtgcagcag
    10441 ataagacagg agaagacact cagctatggt tgagttgtga aagctaatta gatccagcat
    10501 ttcttatgtt gattcagatt gaatgaggta gtgaaaaggg acctgtgctt ttgtaaattc
    10561 tagaaaggca agaggacaat attgaataaa gctgtgttta aacttcattg ctcatatatc
    10621 ttttagtaac tggtaaaccg gcgaagctga taagccaata agagtgctgt ctagaacgca
    10681 tttgcaaacc gttaagagta gcttcttttt gaggcctgga cttctgattc atctatagaa
    10741 gtctattatg attccatcaa ttgcatagct tgtgtacctg gtagaaattg tgtcttggaa
    10801 tgaccctttc gagttattga catggctctg atgaatagaa catgagcccc aaaactaaat
    10861 ccaaaaggaa ttttctatct ttcatccccc acatgtggca agacaagttg gccctttctt
    10921 acccagaggt cttttgtgtg actgcatctt tctcctccgt tctccattgt gtgctttcca
    10981 ttttgtcttt agtgcctata ctgttaggtg ttttcttcac tggcattcac aaatttaagc
    11041 cattgctgcc tcattagcct tgtattttgt gtgcatatca tgtatccaga cctgtatgtt
    11101 cgctttaagc attcttatat cacactgtct cctcatctac catatggtaa atgttaaaac
    11161 tccacatttg tctgcatcag ggaaaatgca tgggcacaca tcctccctcc ctccctctct
    11221 gctctcctcc cttccttcag gcctcttagc attgtttgtt ttcccatttc tgatactact
    11281 actccatgct gaagatttgc catattacta ttttggaaac attgagtgat agaactccta
    11341 gaaaatttgc aaagaaatgt tacatactgt atatcaaact ctcagattct agtgttgaaa
    11401 aagtagccta tactttgcta ttacttatac ctgctgccat agaaaaaaat aagtttattc
    11461 atgacacatt tacatttgat cataaataaa agaaaaaagg gcaccttttt ggagttagtc
    11521 atggtagtca ttagtgatat ttctgaacag ttcttaattt aaaatacttc aaaggaagta
    11581 aaggtcatgg cttagctgaa ggaaatgctc cagaaattgg actgtgtaaa ccatcagtac
    11641 aataatacgc tgtgtatgta tgtgtatata aaatgagaat tatggcataa ttggagcatt
    11701 tgcattaatc aacaaactca cattgagaca aaacttagtt ttacagctgt cttgattaaa
    11761 gccaagtgtt ccatgttgct gtgaagaata gcctctttca aatactttgg aaagtagtta
    11821 cttggaaact tgtaaaggta ttacattttt atatttaaac acctatagag atcttcaatt
    11881 ccttgagtct gagcttgtgg gtggaattct aaatttgtat cataatctgt cttttgtgaa
    11941 acattttgaa aatatgtata tataatattg tatatgcaaa ttgtgttgtt tcacttgtaa
    12001 agggaaaagg cttatttttc tttatatttc tgataacttg ttttgcatat gaccagcact
    12061 gactgaaagg catgtgtagc tgcaaacact gttgcttttt ttgtgaaatg aaaataaaag
    12121 tatttaaata caaa
//
