LOCUS       NM_018368               3900 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens LMBR1 domain containing 1 (LMBRD1), transcript variant
            1, mRNA.
ACCESSION   NM_018368
VERSION     NM_018368.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Kitai,K., Kawaguchi,K., Tomohiro,T., Morita,M., So,T. and
            Imanaka,T.
  TITLE     The lysosomal protein ABCD4 can transport vitamin B12 across
            liposomal membranes in vitro
  JOURNAL   J Biol Chem 296, 100654 (2021)
   PUBMED   33845046
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3900)
  AUTHORS   Fettelschoss,V., Burda,P., Sagne,C., Coelho,D., De Laet,C.,
            Lutz,S., Suormala,T., Fowler,B., Pietrancosta,N., Gasnier,B.,
            Bornhauser,B., Froese,D.S. and Baumgartner,M.R.
  TITLE     Clinical or ATPase domain mutations in ABCD4 disrupt the
            interaction between the vitamin B12-trafficking proteins ABCD4 and
            LMBD1
  JOURNAL   J Biol Chem 292 (28), 11980-11991 (2017)
   PUBMED   28572511
  REMARK    GeneRIF: Data suggest that ABCD4 lysosomal targeting depends on
            co-expression of and interaction with LMBRD1; mutations in LMBRD1
            and ABCD4 that result in cobalamin metabolism disorders cblF and
            cblJ (or mutations in ATPase domain) disrupt interactions between
            LMBRD1 and ABCD4. (LMBRD1 = nuclear export signal-interacting
            protein; ABCD4 = ATP-binding cassette, sub-family D (ALD), member
            4)
REFERENCE   4  (bases 1 to 3900)
  AUTHORS   Kawaguchi,K., Okamoto,T., Morita,M. and Imanaka,T.
  TITLE     Translocation of the ABC transporter ABCD4 from the endoplasmic
            reticulum to lysosomes requires the escort protein LMBD1
  JOURNAL   Sci Rep 6, 30183 (2016)
   PUBMED   27456980
  REMARK    GeneRIF: endogenous ABCD4 was localized to both lysosomes and the
            ER, and its lysosomal localization was disturbed by knockout of
            LMBRD1
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3900)
  AUTHORS   Deme,J.C., Hancock,M.A., Xia,X., Shintre,C.A., Plesa,M., Kim,J.C.,
            Carpenter,E.P., Rosenblatt,D.S. and Coulton,J.W.
  TITLE     Purification and interaction analyses of two human lysosomal
            vitamin B12 transporters: LMBD1 and ABCD4
  JOURNAL   Mol Membr Biol 31 (7-8), 250-261 (2014)
   PUBMED   25535791
  REMARK    GeneRIF: Results propose a model whereby membrane-bound LMBD1 and
            ABCD4 facilitate the vectorial delivery of lysosomal vitamin B12 to
            cytoplasmic MMACHC.
REFERENCE   6  (bases 1 to 3900)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 3900)
  AUTHORS   Rutsch,F., Gailus,S., Miousse,I.R., Suormala,T., Sagne,C.,
            Toliat,M.R., Nurnberg,G., Wittkampf,T., Buers,I., Sharifi,A.,
            Stucki,M., Becker,C., Baumgartner,M., Robenek,H., Marquardt,T.,
            Hohne,W., Gasnier,B., Rosenblatt,D.S., Fowler,B. and Nurnberg,P.
  TITLE     Identification of a putative lysosomal cobalamin exporter altered
            in the cblF defect of vitamin B12 metabolism
  JOURNAL   Nat Genet 41 (2), 234-239 (2009)
   PUBMED   19136951
  REMARK    GeneRIF: LMBRD1 is the gene underlying the cblF defect of cobalamin
            metabolism and suggests that LMBD1 is a lysosomal membrane exporter
            for cobalamin.
REFERENCE   8  (bases 1 to 3900)
  AUTHORS   Wang,Y.H., Chang,S.C., Huang,C., Li,Y.P., Lee,C.H. and Chang,M.F.
  TITLE     Novel nuclear export signal-interacting protein, NESI, critical for
            the assembly of hepatitis delta virus
  JOURNAL   J Virol 79 (13), 8113-8120 (2005)
   PUBMED   15956556
REFERENCE   9  (bases 1 to 3900)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 3900)
  AUTHORS   Sloan,J.L., Carrillo,N., Adams,D. and Venditti,C.P.
  TITLE     Disorders of Intracellular Cobalamin Metabolism
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301503
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA028310.1, BC047073.1,
            AL583831.6, BJ995961.1 and AL358133.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 15, 2018 this sequence version replaced NM_018368.3.
            
            Summary: This gene encodes a lysosomal membrane protein that may be
            involved in the transport and metabolism of cobalamin. This protein
            also interacts with the large form of the hepatitis delta antigen
            and may be required for the nucleocytoplasmic shuttling of the
            hepatitis delta virus. Mutations in this gene are associated with
            the vitamin B12 metabolism disorder termed,
            homocystinuria-megaloblastic anemia complementation type
            F.[provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.26697.1,
                                           SRR1660803.118843.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000649934.3/ ENSP00000497690.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DA028310.1         148-161
            15-1311             BC047073.1         1-1297
            1312-1312           AL583831.6         16212-16212         c
            1313-2053           BC047073.1         1299-2039
            2054-2161           BJ995961.1         1-108               c
            2162-3900           AL358133.11        81211-82949         c
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q13"
     gene            1..3900
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="LMBR1 domain containing 1"
                     /db_xref="GeneID:55788"
                     /db_xref="HGNC:HGNC:23038"
                     /db_xref="MIM:612625"
     exon            1..198
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1752
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable lysosomal cobalamin transporter; liver
                     regeneration p-53 related protein; hepatitis delta
                     antigen-L interacting protein; HDAg-L-interacting protein
                     NESI; nuclear export signal-interacting protein; lysosomal
                     cobalamin transport escort protein LMBD1"
                     /codon_start=1
                     /product="lysosomal cobalamin transport escort protein
                     LMBD1 isoform 1"
                     /protein_id="NP_060838.3"
                     /db_xref="CCDS:CCDS4969.1"
                     /db_xref="GeneID:55788"
                     /db_xref="HGNC:HGNC:23038"
                     /db_xref="MIM:612625"
                     /translation="MATSGAASAELVIGWCIFGLLLLAILAFCWIYVRKYQSRRESEV
                     VSTITAIFSLAIALITSALLPVDIFLVSYMKNQNGTFKDWANANVSRQIEDTVLYGYY
                     TLYSVILFCVFFWIPFVYFYYEEKDDDDTSKCTQIKTALKYTLGFVVICALLLLVGAF
                     VPLNVPNNKNSTEWEKVKSLFEELGSSHGLAALSFSISSLTLIGMLAAITYTAYGMSA
                     LPLNLIKGTRSAAYERLENTEDIEEVEQHIQTIKSKSKDGRPLPARDKRALKQFEERL
                     RTLKKRERHLEFIENSWWTKFCGALRPLKIVWGIFFILVALLFVISLFLSNLDKALHS
                     AGIDSGFIIFGANLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWF
                     FWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIETNIT
                     SDNHKGNSTLSVPKRCDADAPEDQCTVTRTYLFLHKFWFFSAAYYFGNWAFLGVFLIG
                     LIVSCCKGKKSVIEGVDEDSDISDDEPSVYSA"
     exon            199..375
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            376..436
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            437..534
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            535..602
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            603..691
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            692..765
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            766..891
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1044
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1109
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1212
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1317
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1467
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1546
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1638
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..3900
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2032..2037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="hexamer: ATTAAA"
     polyA_site      2053
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="major polyA site"
     regulatory      2083..2088
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="hexamer: AATAAA"
     polyA_site      2108
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
     polyA_site      2137
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
     regulatory      2173..2178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="hexamer: AATATA"
     polyA_site      2198
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
     regulatory      3877..3882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
                     /note="hexamer: AATAAA"
     polyA_site      3900
                     /gene="LMBRD1"
                     /gene_synonym="C6orf209; LMBD1; MAHCF; NESI"
ORIGIN      
        1 acttgctcct ccccctccgc ccctttaacc tttagggtgc gcgggtgcag tatatctcgc
       61 gctctctccc ctttccccct cccctttccc caccccgggc gctcaggttg gtctggaccg
      121 gaagcgaaga tggcgacttc tggcgcggcc tcggcggagc tggtgatcgg ctggtgcata
      181 ttcggcctct tactactggc tattttggca ttctgctgga tatatgttcg taaataccaa
      241 agtcggcggg aaagtgaagt tgtctccacc ataacagcaa ttttttctct agcaattgca
      301 cttatcacat cagcacttct accagtggat atatttttgg tttcttacat gaaaaatcaa
      361 aatggtacat ttaaggactg ggctaatgct aatgtcagca gacagattga ggacactgta
      421 ttatacggtt actatacttt atattctgtt atattgttct gtgtgttctt ctggatccct
      481 tttgtctact tctattatga agaaaaggat gatgatgata ctagtaaatg tactcaaatt
      541 aaaacggcac tcaagtatac tttgggattt gttgtgattt gtgcactgct tcttttagtt
      601 ggtgcctttg ttccattgaa tgttcccaat aacaaaaatt ctacagagtg ggaaaaagtg
      661 aagtccctat ttgaagaact tggaagtagt catggtttag ctgcattgtc attttctatc
      721 agttctctga ccttgattgg aatgttggca gctataactt acacagccta tggcatgtct
      781 gcgttacctt taaatctgat aaaaggcact agaagcgctg cttatgaacg tttggaaaac
      841 actgaagaca ttgaagaagt agaacaacac attcaaacga ttaaatcaaa aagcaaagat
      901 ggtcgacctt tgccagcaag ggataaacgc gccttaaaac aatttgaaga aaggttacga
      961 acacttaaga agagagagag gcatttagaa ttcattgaaa acagctggtg gacaaaattt
     1021 tgtggcgctc tgcgtcccct gaagatcgtc tggggaatat ttttcatctt agttgcattg
     1081 ctgtttgtaa tttctctctt cttgtcaaat ttagataaag ctcttcattc agctggaata
     1141 gattctggtt tcataatttt tggagctaac ctgagtaatc cactgaatat gcttttgcct
     1201 ttactacaaa cagttttccc tcttgattat attcttataa caattattat tatgtacttt
     1261 atttttactt caatggcagg aattcgaaat attggcatat ggttcttttg gattagatta
     1321 tataaaatca gaagaggtag aaccaggccc caagcactcc tttttctctg catgatactt
     1381 ctgcttattg tccttcacac tagctacatg atttatagtc ttgctcccca atatgttatg
     1441 tatggaagcc aaaattactt aatagagact aatataactt ctgataatca taaaggcaat
     1501 tcaacccttt ctgtgccaaa gagatgtgat gcagatgctc ctgaagatca gtgtactgtt
     1561 acccggacat acctattcct tcacaagttc tggttcttca gtgctgctta ctattttggt
     1621 aactgggcct ttcttggggt atttttgatt ggattaattg tatcctgttg taaagggaag
     1681 aaatcggtta ttgaaggagt agatgaagat tcagacataa gtgatgatga gccctctgtc
     1741 tattctgctt gacagccttc tgtcttaaag gttttataat gctgactgaa tatctgttat
     1801 gcatttttaa agtattaaac taacattagg atttgctaac tagctttcat caaaaatggg
     1861 agcatggcta taagacaact atattttatt atatgttttc tgaagtaaca ttgtatcata
     1921 gattaacatt ttaaattacc ataatcatgc tatgtaaata taagactact ggctttgtga
     1981 gggaatgttt gtgcaaaatt ttttcctcta atgtataata gtgttaaatt gattaaaaat
     2041 cttccagaat taatattccc ttttgtcact ttttgaaaac ataataaatc atctgtatct
     2101 gtgccttagg ttctccagag tgatgtggaa ttttaaagtg tctctctctg attgcctcca
     2161 aataatattt acaatatagc tgatgaatgt tactcccata tgcaaattgt tttcagtttt
     2221 ctgcataaaa tctttagcac cactcaagta gatttcatga aaagatttca aactctagtt
     2281 agtgctctaa gagcatggcg ttccaagatg tgttatattg ttcaaatatc agtccattag
     2341 ataacttaca acctctacag tatttaaggc atacatattg ttataggtag cttttgtttt
     2401 gcatctgacc tcaaaatgtg tatttcaata taaatagggg tattgtcaag aataattaat
     2461 cctggaaaaa aaaatcagtt tatggattaa tttaagatat atggcatcat attttggttt
     2521 tagagataat ttttcattct aaagatttga aatttgcaaa agatgtaatg aaagctgtat
     2581 gacatcagta gggtattttg aacacattct ggatagtgag aaataataaa aaattatatt
     2641 ctagtttact aacataagca cattagaact cataaaaaag tatatatcat gatccttgag
     2701 gtaaaatatc attaagtttc tgaaaaaaag tttaggaatt aacaactata tctaagttaa
     2761 ttattctctc ccttaatttt tgtgccattt ttctctgttc tgattcttca tctctcttga
     2821 taaaccttca cagctttcgt atttggcttc tgcttctgca actacccttt agcattattc
     2881 accaggattt cattcccttc tgttcttatt ttacattttc ttttttcttt ttttttaaga
     2941 tggagtttca ctcttgtcgc ccaggctgga gtgcaatggc acgatctcag ctcactgcaa
     3001 cctccccttc atgcattcaa gcaattctcc tgcctcagcc tcctgagtag ctgggattac
     3061 aggcatgtgc caccatgcct ggctagtttt tgtattttta gcagagatgg ggtttcacca
     3121 tcttggccag gctggtcttg aactcctgac ctcatgatcc acccacagtg gcctcccaaa
     3181 gtgctgggat tacaggcata agccaccacg ctcggcctta tattacattt tctttgtgga
     3241 caatctcaca tacccttggc ttctatctat actacttgtt cttaagctct gaactttgag
     3301 ttatctttta gctagaaatt actgcttcaa ctgtaacatt ttgaaaattg aagtattcac
     3361 ttttgtgggg gcaaactaag ccttcttctg cattcatttt tttgacaaat ggcaccgcag
     3421 acacttgatt gcctaaacaa agaatcaggt agatctccat atctcttctg tataactgac
     3481 tcatcaaaca atcatgtcat gttaatttct acttcctatc tttccatttg tcccttcttc
     3541 tccactttct atgttatagg ctgaattgtg tccccttaca attcatatgt tgaaatcttg
     3601 tcccctggta cttcatagaa taatacttta tgtggagata gggcctttta aaagtaatta
     3661 aagtaaaatg aggctattgc gtgaagccca aatccaatat gactggtgtt cttataagaa
     3721 gggggagaga catctagaat gtgtatgtac aaagaagagg ccatgtgagg atgcaatgag
     3781 aaggcagtgg tctgcaagcc aacaggagag gcctcaggag aatccagtgt tgtcaaccct
     3841 ttaatcttga ggttccagct tccagatctg tgagaaaata aatttctgtt gtttaagcca
//
