LOCUS       NM_022458               7950 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens limb development membrane protein 1 (LMBR1),
            transcript variant 2, mRNA.
ACCESSION   NM_022458
VERSION     NM_022458.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Xia,L., Yang,Z. and Hu,C.
  TITLE     PAR1 Activation, via LMBR1/BMP Axis, Promotes the Osteogenesis of
            Periodontal Ligament Stem Cells (PDLSCs) and Alleviates the
            Inhibitory Effect of Sodium Butyrate on PDLSCs Osteogenesis
  JOURNAL   Discov Med 36 (187), 1657-1671 (2024)
   PUBMED   39190381
  REMARK    GeneRIF: PAR1 Activation, via LMBR1/BMP Axis, Promotes the
            Osteogenesis of Periodontal Ligament Stem Cells (PDLSCs) and
            Alleviates the Inhibitory Effect of Sodium Butyrate on PDLSCs
            Osteogenesis.
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Potuijt,J.W.P., Hoogeboom,J., de Graaff,E., van Nieuwenhoven,C.A.
            and Galjaard,R.J.H.
  TITLE     Variable expression of subclinical phenotypes instead of reduced
            penetrance in families with mild triphalangeal thumb phenotypes
  JOURNAL   J Med Genet 57 (10), 660-663 (2020)
   PUBMED   32179704
  REMARK    GeneRIF: Variable expression of subclinical phenotypes instead of
            reduced penetrance in families with mild triphalangeal thumb
            phenotypes.
REFERENCE   3  (bases 1 to 7950)
  AUTHORS   Xu,J., Wu,J., Teng,X., Cai,L., Yuan,H., Chen,X., Hu,M., Wang,X.,
            Jiang,N. and Chen,H.
  TITLE     Large duplication in LMBR1 gene in a large Chinese pedigree with
            triphalangeal thumb polysyndactyly syndrome
  JOURNAL   Am J Med Genet A 182 (9), 2117-2123 (2020)
   PUBMED   32662247
  REMARK    GeneRIF: Large duplication in LMBR1 gene in a large Chinese
            pedigree with triphalangeal thumb polysyndactyly syndrome.
REFERENCE   4  (bases 1 to 7950)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 7950)
  AUTHORS   Xu,C., Yang,X., Zhou,H., Li,Y., Xing,C., Zhou,T., Zhong,D.,
            Lian,C., Yan,M., Chen,T., Liao,Z., Gao,B., Su,D., Wang,T.,
            Sharma,S., Mohan,C., Ahituv,N., Malik,S., Li,Q.Z. and Su,P.
  TITLE     A novel ZRS variant causes preaxial polydactyly type I by increased
            sonic hedgehog expression in the developing limb bud
  JOURNAL   Genet Med 22 (1), 189-198 (2020)
   PUBMED   31395945
  REMARK    GeneRIF: Results identify a novel g.101779T>A variant segregating
            with all individuals affected with preaxial polydactyly type
            1(PPDI).
REFERENCE   6  (bases 1 to 7950)
  AUTHORS   Dundar,M., Gordon,T.M., Ozyazgan,I., Oguzkaya,F., Ozkul,Y.,
            Cooke,A., Wilkinson,A.G., Holloway,S., Goodman,F.R. and Tolmie,J.L.
  TITLE     A novel acropectoral syndrome maps to chromosome 7q36
  JOURNAL   J Med Genet 38 (5), 304-309 (2001)
   PUBMED   11333865
REFERENCE   7  (bases 1 to 7950)
  AUTHORS   Ianakiev,P., van Baren,M.J., Daly,M.J., Toledo,S.P.,
            Cavalcanti,M.G., Neto,J.C., Silveira,E.L., Freire-Maia,A.,
            Heutink,P., Kilpatrick,M.W. and Tsipouras,P.
  TITLE     Acheiropodia is caused by a genomic deletion in C7orf2, the human
            orthologue of the Lmbr1 gene
  JOURNAL   Am J Hum Genet 68 (1), 38-45 (2001)
   PUBMED   11090342
REFERENCE   8  (bases 1 to 7950)
  AUTHORS   Clark,R.M., Marker,P.C. and Kingsley,D.M.
  TITLE     A novel candidate gene for mouse and human preaxial polydactyly
            with altered expression in limbs of Hemimelic extra-toes mutant
            mice
  JOURNAL   Genomics 67 (1), 19-27 (2000)
   PUBMED   10945466
REFERENCE   9  (bases 1 to 7950)
  AUTHORS   Heus,H.C., Hing,A., van Baren,M.J., Joosse,M., Breedveld,G.J.,
            Wang,J.C., Burgess,A., Donnis-Keller,H., Berglund,C., Zguricas,J.,
            Scherer,S.W., Rommens,J.M., Oostra,B.A. and Heutink,P.
  TITLE     A physical and transcriptional map of the preaxial polydactyly
            locus on chromosome 7q36
  JOURNAL   Genomics 57 (3), 342-351 (1999)
   PUBMED   10329000
REFERENCE   10 (bases 1 to 7950)
  AUTHORS   Hing,A.V., Helms,C., Slaugh,R., Burgess,A., Wang,J.C., Herman,T.,
            Dowton,S.B. and Donis-Keller,H.
  TITLE     Linkage of preaxial polydactyly type 2 to 7q36
  JOURNAL   Am J Med Genet 58 (2), 128-135 (1995)
   PUBMED   8533803
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA238712.1, BC017663.1,
            AC005534.2 and H18430.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_022458.3.
            
            Summary: This gene encodes a member of the LMBR1-like membrane
            protein family. Another member of this protein family has been
            shown to be a lipocalin transmembrane receptor. A highly conserved,
            cis-acting regulatory module for the sonic hedgehog gene is located
            within an intron of this gene. Consequently, disruption of this
            genic region can alter sonic hedgehog expression and affect limb
            patterning, but it is not known if this gene functions directly in
            limb development. Mutations and chromosomal deletions and
            rearrangements in this genic region are associated with acheiropody
            and preaxial polydactyly, which likely result from altered sonic
            hedgehog expression. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.105364.1,
                                           SRR1803613.115062.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000353442.10/ ENSP00000326604.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DA238712.1         26-61
            37-2802             BC017663.1         1-2766
            2803-4751           AC005534.2         33960-35908
            4752-4865           H18430.1           20-133              c
            4866-7950           AC005534.2         36023-39107
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.3"
     gene            1..7950
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="limb development membrane protein 1"
                     /db_xref="GeneID:64327"
                     /db_xref="HGNC:HGNC:13243"
                     /db_xref="MIM:605522"
     exon            1..256
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..1663
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="isoform b is encoded by transcript variant 2; limb
                     region 1 protein homolog; differentiation-related gene 14
                     protein; limb region 1 homolog"
                     /codon_start=1
                     /product="limb region 1 protein homolog isoform b"
                     /protein_id="NP_071903.2"
                     /db_xref="CCDS:CCDS5945.1"
                     /db_xref="GeneID:64327"
                     /db_xref="HGNC:HGNC:13243"
                     /db_xref="MIM:605522"
                     /translation="MEGQDEVSAREQHFHSQVRESTICFLLFAILYVVSYFIITRYKR
                     KSDEQEDEDAIVNRISLFLSTFTLAVSAGAVLLLPFSIISNEILLSFPQNYYIQWLNG
                     SLIHGLWNLASLFSNLCLFVLMPFAFFFLESEGFAGLKKGIRARILETLVMLLLLALL
                     ILGIVWVASALIDNDAASMESLYDLWEFYLPYLYSCISLMGCLLLLLCTPVGLSRMFT
                     VMGQLLVKPTILEDLDEQIYIITLEEEALQRRLNGLSSSVEYNIMELEQELENVKTLK
                     TKLERRKKASAWERNLVYPAVMVLLLIETSISVLLVACNILCLLVDETAMPKGTRGPG
                     IGNASLSTFGFVGAALEIILIFYLMVSSVVGFYSLRFFGNFTPKKDDTTMTKIIGNCV
                     SILVLSSALPVMSRTLGITRFDLLGDFGRFNWLGNFYIVLSYNLLFAIVTTLCLVRKF
                     TSAVREELFKALGLHKLHLPNTSRDSETAKPSVNGHQKAL"
     exon            257..329
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            330..369
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            370..509
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            510..613
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            614..740
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            741..809
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            810..874
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            875..947
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1028
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1105
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1183
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1257
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1348
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1415
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1577
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..7950
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2782..2787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: AATAAA"
     polyA_site      2802
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="major polyA site"
     regulatory      4200..4205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: AATAAA"
     polyA_site      4222
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="major polyA site"
     regulatory      4838..4843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: ATTAAA"
     polyA_site      4860
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
     regulatory      5779..5784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: AATAAA"
     polyA_site      5802
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
     regulatory      6658..6663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: AATACA"
     polyA_site      6679
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
     regulatory      7872..7877
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
                     /note="hexamer: AATAAA"
     polyA_site      7910
                     /gene="LMBR1"
                     /gene_synonym="ACHP; C7orf2; DIF14; LSS; PPD2; THYP; TPT;
                     TPTPS; ZRS"
ORIGIN      
        1 agtcagagct gctcgtctga ggctgctgag gcgacggccg gtgtcgtggt cgcggtacct
       61 gttccaacac ggctcgcggg cccgtgccgg ctccggtccc cggcgcggct gtccgagccc
      121 ctgcggcggg cggacgatgg tgtggcggag cacgcggacg cgggcggcgc ggcggcgggc
      181 atgaaggagg atggaagggc aggacgaggt gtcggcgcgg gagcagcact tccacagcca
      241 agtgcgggag tccacgatat gtttccttct ttttgccatt ctctacgttg tttcctactt
      301 catcatcaca agatacaaga gaaaatcaga tgaacaagaa gatgaagatg ccatcgtcaa
      361 caggatttcg ttgtttttga gcacgttcac tctcgcagtg tcagctgggg ctgttttgct
      421 tttacccttc tcaatcatca gcaatgaaat cctgctttct tttcctcaga actactatat
      481 tcagtggcta aatggctccc tgattcatgg tttgtggaat cttgcttccc ttttttccaa
      541 cctttgttta tttgtattga tgccctttgc ctttttcttt ctggaatcag aaggctttgc
      601 tggcctgaaa aagggaatcc gagcccgcat tttagagact ttggtcatgc ttcttcttct
      661 tgcgttactc attcttggga tagtgtgggt agcttcagca ctcattgaca acgatgccgc
      721 aagcatggaa tctttatatg atctctggga gttctatcta ccctatttat attcctgtat
      781 atcattgatg ggatgtttgt tacttctctt gtgtacacca gttggccttt ctcgtatgtt
      841 cacagtgatg ggtcagttgc tagtgaagcc aacaattctt gaagacctgg atgaacaaat
      901 ttatatcatt accttagagg aagaagcact ccagagacga ctaaatgggc tgtcttcatc
      961 ggtggaatac aacataatgg agttggaaca agaacttgaa aatgtaaaga ctcttaagac
     1021 aaaattagag aggcgaaaaa aggcttcagc atgggaaaga aatttggtgt atcccgctgt
     1081 tatggttctc cttcttattg agacatccat ctcggtcctc ttggtggctt gtaatattct
     1141 ttgcctattg gttgatgaaa cagcaatgcc aaaaggaaca agggggcctg gaataggaaa
     1201 tgcctctctt tctacgtttg gttttgtggg agctgcgctt gaaatcattt tgattttcta
     1261 tcttatggtg tcctctgttg tcggcttcta tagccttcga ttttttggaa actttactcc
     1321 caagaaagat gacacaacta tgacaaagat cattggaaat tgtgtgtcca tcttggtttt
     1381 gagctctgct ctgcctgtga tgtcgagaac actgggaatc actagatttg atctacttgg
     1441 cgactttgga aggtttaatt ggctgggaaa tttctatatt gtattatcct acaatttgct
     1501 ttttgctatt gtgacaacat tgtgtctggt ccgaaaattc acctctgcag ttcgagaaga
     1561 acttttcaag gccctagggc ttcataaact tcacttacca aatacttcaa gggattcaga
     1621 aacagccaag ccttctgtaa atgggcatca gaaagcactg tgagacgcac agacggcgtc
     1681 ttctgccacc aagagacccg agaactccag attcacgaca ttcctgtccc atgtagaagc
     1741 atttccattc aaccgtggcc cctcttcaga acctagacct atcagtgcca tttttttttc
     1801 ataatctacg aagaacttgg ctatggctga tcttttttaa atttaacttt ctgatggacc
     1861 ctgtagtttc cagttaagtg cagattcctt acagacatat agaacagcgc attcttctgt
     1921 agacatttgc tcatgttggt aaatacaatc acccatatga aaaaattgtt ttcacctgat
     1981 atgaaaatgt tagaaaaggc aaactccggg acttctaaag atttacttaa atcccattat
     2041 gtactttatt cagaatgtag aagctgactt gaaaggcatc cttggtacta agtgaagctt
     2101 attcagaaaa tgcatttttc aaatgcaatg gcaactgctt gtagatatca tttttgcagt
     2161 gtatgttgga gctgtaatgg ttgcaattat gtttcttatt tccttaaaag caaaaagcgt
     2221 agtttctgat ttatgttata gaatgatact gattagactt tgagccaagg ggaaaatact
     2281 aaattctttt aaacctggag ccttagagag ccacaggaat atcttctgtt gtacagtcta
     2341 ataagctgtg gtaggaagta tcatgtaatc acagtttaat gacagtttat gtatatatat
     2401 aattcagtat tccctctgat aacatagttg ccagtgttta atacacttgt aacttggatt
     2461 tttaccttat aggctatatg tatactcagt tttttaaagc atttttttca gagatcactt
     2521 aattccccat gcttctgcaa tgcatataaa aactataaat gccgagtggt agaaactcct
     2581 ctttcttcat agtcctcagg ctttggttac atttgcatat gccatttgaa gcctccagct
     2641 tttaccagtt taacatccaa agttcacagc atcagcattc atggtgtaag aacagttttg
     2701 cagtataaca cgatctgata atcattcagt tattaaattg taaataatta ttgggatggt
     2761 ttcttggctt taagtccact gaataaaaac tatgaaattg cactctgtgt caaccatcca
     2821 ctaggataga ataccgaaat ctgtgcatgc aaaaatagga gatgggccca tttgcacaca
     2881 attcgtagtt atgcagtctg ctatataaat atgttcacat gcactgtgtg tatgaaaata
     2941 gatggtctgt gttcagacaa aagtaaaaca tttttttcaa attgttacat ttaaaggttt
     3001 tctgggagaa atttatgaaa cgcaggctgt gtctatttga catcagaaat ttccacttta
     3061 aaccaaaata ataagaaact ttaatctgta tatttacaac ctttgttgag tacacttccc
     3121 ccttatttat acgtctgcat ttccttccga gcttcacatc tttctaaaat gcagcttggt
     3181 tttaaaataa aagaacattc attttgtgat tctaaacaag cttcagtaaa taccaccagt
     3241 atagtactgg tgaatttctc agcataaaat cgacatacct aaaaagttaa taaaattcag
     3301 ctcttttcca atttcattgt tatgcctatt gaagtattaa ttgccaggtt tgatttttag
     3361 tgaagcttgg agtccatact ttgagcagac caagtgaagg gaagaacaga aagaaactca
     3421 ggagtagagt aatatcactt ctcacttaca ccactttcag gcacatccaa agagttccta
     3481 gatacttgga aaatgtctga aaatttttaa gtaaaatact aaacttttca gtgtttagct
     3541 caactttttg ttcatttgga agtttctctc catccgagga cttaagccag ttttggattt
     3601 gtaagccctg agtacaatac acttcctgga ggcatcctca ctgctgttga agcaaaggat
     3661 atgcatgggg tggaaggacg gcttcgaacc tgggactcat atgccttgag aacaaataga
     3721 ttgttacagc cttgggctgc tgcgtaatca cggttcctcg aggctcttcc tgagcacatg
     3781 cccaagcatc tgcctctgga gagactgact ccaaatgcag gtgcttccat tggagctagg
     3841 tcggaggctg ctttatatga cgaactccag aaatggatgc cagaatacgg aggccaaacg
     3901 ttctgagtcc tggtaaggac agtcgctctg ggggtcctca ttttactgca gttcctgcac
     3961 gccagtgaaa gagaggagat agaccctgga aggcagagct gcagatgctc atcatcaggt
     4021 caattctgga gctacagttt tgtttctgac tggataggga tgcaccagtg actgtcacat
     4081 caagcagtcc ttttattctc tctcctttag tatcgatttt aaagggcatt aggcactatg
     4141 gttccagagt ttcttgggga aaacttgcag attcttatta attggttctg caatacttaa
     4201 ataaattatt ttacaattat aagttttcag attataacat ttgtattaat ttttactgat
     4261 tttccaagat acttcttaga tttactattt acgtagcttt atgtacattc tctgtaaaaa
     4321 tagacctcta aatatgaggc tttacatgaa atttgtacac acatacacac taatgttagc
     4381 tccttaaatt gctgcactaa ggtgctggtt agtagagatg gacggagcct ctcgcgtttt
     4441 gctctcagat gtgttaaagg cgcacgtgta cctgctctca gcggcagtgc ggcctcccca
     4501 tctgctgggt gcccatggcc ctccctgcag cctcagtgat gacctcgtct gccagggaca
     4561 caggttttca tcatttacag gctcttatgt gctagttttg ttggtagcac gttatttaat
     4621 gcataaaggc agaattctta caagtttttt tttttaatgt gaacatagat gcagcaccga
     4681 ctttttaaac ttgaaaaaac tggtataatg ttaactttta aaaataacat ttggacacac
     4741 tagtaattga tttttgttta cagattgttt tgtttacaaa ttgttagtct ttgtttctat
     4801 gagatacttt tagtgtgact ttttaaatgt cttagaaatt aaaagttgta caaaaagtga
     4861 tttcatattt ggtttataag catttatatg tggggtttat ttgttctttt gttttttcca
     4921 tcttaaatat tatcatggct aaaacttaag ggtatttata gtttaattcc atttcagttt
     4981 tatagagggc agtaattatt ctgatgaatg ttgaattaag aaatggatat tttctttctc
     5041 tgttgtgcag ttattggtag atcaatttct tataacccac aatgtagcat caataattga
     5101 tagcatgtat tttatttaat tacttgaatt atttagactt gatttctcta attttttcca
     5161 taaaaggact gaacagcacc tacttgtggt ctggacagct taacccagag ttcctggaag
     5221 aataaatgct gttagcatct ggttaattta ctggcagaca gaagcctact tacagtggct
     5281 ttcaactttt tgaccacagt aagaaataac ccattacaca cacacacaca cacacactct
     5341 ctctctctct ctctctctct ctctgtctct cagatacgta taagcaaaaa tttaacaaga
     5401 cagtacttgt ttttcctaag tgtgcatgct ggcatcttct gttttattat ttttaaaaat
     5461 actagacaca gacactaagt tactttgtgg cctcctaata gatggcagac ttcagtttga
     5521 aaagcatccc tttggaatgt ggtttaaaag aagaaataat acaaagacct ttttggagtt
     5581 tttttttttt ttggtttttg aatattgttt aggtaaaaat tattgctgca aacacaactg
     5641 aagcaaaagt actgtgtacg caaattactt gttgcactgt agaaaatgta atatcaggag
     5701 aagttcctaa aactcacaaa tatatacaaa atttcaaaac ttggtgattg atccatatgt
     5761 cttgaaggaa tattaggcaa taaacttacc ttttcaagag aagttattta acttccaatt
     5821 ctgtgttctt taattaggaa aaaatttttt caaggttaac tataatcaaa taggaacttc
     5881 acacaaaacc tcattattct tggatatgga tttggtgttc tgctgttact tctaaaggtg
     5941 gtacagaagt caagtttatg agcctggatt atgaacagca gggcggccct gatgtgagtg
     6001 gttgtggagg actagagtga tgtagaagaa tctgttgtgt ctgagtgtga ggaggtggag
     6061 agtcacgtgt gggctatggg tctgagaact gaagttttaa cagttactaa aactgttttg
     6121 tggctgttgt aatgtcttct cctttccttt cctttgaaaa gctgttactg aatgaacgat
     6181 gccgcttaca aacactgaca cttcagaacc aagcatattt gatactgctg tagctattta
     6241 tattgattct gcaacttctc tttaacagca tcttgaagtc atccacagtt gttctatatg
     6301 gactccacag tagagtcaga gaactaagtg acaagtggag catcgtcctc ctcctgctca
     6361 cttcctcctc gccacctctc tggcctggct atccttcagt ctgaagagtg attcactttt
     6421 tcttttcttt ttagtcttta ctgtggaatc tcatcaaaga cttggaagca ggggtggtga
     6481 gaggggcctg gggcgtggtg tggagggtcg agttctttgt cgtcccacag tcacccccag
     6541 gaccaccttg cgaagccgaa aatacacatc tgcattttac cgacaaggaa cgccactgag
     6601 aaagctgcag acatttgcct ggggtcacgc ttacagtggg cagatgaact ggatgtaaat
     6661 acaggccagt acaaccgcag tctcattttt tctttgtttt aggttttcag tttcttgttc
     6721 attcatgtac cttacagttc tattatctgg ttgataaaga ttcatgacag gtatcctcat
     6781 tctagattat aacctttatt cttatcaaat gcctccagtc ccacaattcg ctaagaattc
     6841 tctttctgga cttgatagtg atttaatgat tccccccgcc catggaatgg taggctccta
     6901 attaagtgga tttaaaggca aaaagggatg tgtgagaagg cacaagaagt ggcttcatct
     6961 tgcaaggtga ctgtcagaca agtgggttcc aacctgttaa aataagggag gaatggccac
     7021 atcccgtagg gactgctgta gctgaccagg aagacacaat gagggcagca gacagggctc
     7081 tccctgtccg ctgagtcctc ccatgaactc atgtttccaa aggccccact ctttcttggt
     7141 cagtgtccca gtttttatag aagagacctg atgaggctgt ggagtaattg tataaaagct
     7201 tgcactgttt tcctgacttg acctgacaat gtggcgttat cttcaaattg tccagagaag
     7261 tagctacttc attcaggtcc tttttagcgt tctgtggttg caacttggtc tcgcaagctt
     7321 tcgatgggcg cgccattcca tggccacttt tcagtaagaa aattgcctga ttttctgtta
     7381 actgtcacag gctgccctgg accattcctc agaactcatg ggattttcgc gaccctcatc
     7441 ctcagtgaag ttagatgacc attcctgcct ttcatcccac cgattttcct aagtatctgt
     7501 tgcctgcaac taacactagt tcctgtgtca gtaaagtact gcacttggtt gcaaagaata
     7561 gagatggact ctgccaacat aggcacggaa ggtttacggg gtggatggca tggctcacag
     7621 atgtgatgag aaagccccgg aagaagtgtt gggcacagcc ttaggcacaa cctttcctgg
     7681 gctcttccca tcactgaatg atccatgtat ctccgggact ctccttctag atccaaagtt
     7741 tagggcggcg gaatcggact gctctggcct ttgacacgtg cctgtgcttt gcctgtcaac
     7801 gctatgggga gagagatgtc agcttacaga aaattgaggt tctgtgattc agtttccctc
     7861 tgctctctta gaataaaagt gcttactcat tgtttaaagc attctccaaa ttagtctgca
     7921 aattttttga tagtatacat ccttaaaaaa
//
