LOCUS       NM_194282               6121 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens lin-54 DREAM MuvB core complex component (LIN54),
            transcript variant 1, mRNA.
ACCESSION   NM_194282 XM_059594
VERSION     NM_194282.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Marceau,A.H., Felthousen,J.G., Goetsch,P.D., Iness,A.N., Lee,H.W.,
            Tripathi,S.M., Strome,S., Litovchick,L. and Rubin,S.M.
  TITLE     Structural basis for LIN54 recognition of CHR elements in cell
            cycle-regulated promoters
  JOURNAL   Nat Commun 7, 12301 (2016)
   PUBMED   27465258
  REMARK    GeneRIF: the DNA-binding properties of LIN54 and the structural
            basis for recognition of a cell cycle genes homology region
            sequence, are described.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6121)
  AUTHORS   Matsuo,T., Kuramoto,H., Kumazaki,T., Mitsui,Y. and Takahashi,T.
  TITLE     LIN54 harboring a mutation in CHC domain is localized to the
            cytoplasm and inhibits cell cycle progression
  JOURNAL   Cell Cycle 11 (17), 3227-3236 (2012)
   PUBMED   22895175
  REMARK    GeneRIF: LIN54 harboring a mutation in CHC domain is localized to
            the cytoplasm and inhibits cell cycle progression.
REFERENCE   4  (bases 1 to 6121)
  AUTHORS   Litovchick,L., Florens,L.A., Swanson,S.K., Washburn,M.P. and
            DeCaprio,J.A.
  TITLE     DYRK1A protein kinase promotes quiescence and senescence through
            DREAM complex assembly
  JOURNAL   Genes Dev 25 (8), 801-813 (2011)
   PUBMED   21498570
REFERENCE   5  (bases 1 to 6121)
  AUTHORS   Schmit,F., Cremer,S. and Gaubatz,S.
  TITLE     LIN54 is an essential core subunit of the DREAM/LINC complex that
            binds to the cdc2 promoter in a sequence-specific manner
  JOURNAL   FEBS J 276 (19), 5703-5716 (2009)
   PUBMED   19725879
  REMARK    GeneRIF: the cysteine-rich CXC domain of LIN54 is a novel
            DNA-binding domain that binds to the cdc2 promoter in a
            sequence-specific manner
REFERENCE   6  (bases 1 to 6121)
  AUTHORS   Litovchick,L., Sadasivam,S., Florens,L., Zhu,X., Swanson,S.K.,
            Velmurugan,S., Chen,R., Washburn,M.P., Liu,X.S. and DeCaprio,J.A.
  TITLE     Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein
            complex represses human cell cycle-dependent genes in quiescence
  JOURNAL   Mol Cell 26 (4), 539-551 (2007)
   PUBMED   17531812
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021105.13.
            
            On Nov 23, 2018 this sequence version replaced NM_194282.3.
            
            Summary: LIN54 is a component of the LIN, or DREAM, complex, an
            essential regulator of cell cycle genes (Schmit et al., 2009
            [PubMed 19725879]).[supplied by OMIM, Dec 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX640966.1, SRR1660807.215599.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340417.8/ ENSP00000341947.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-340               AC021105.13        39143-39482
            341-1056            AC021105.13        65090-65805
            1057-1180           AC021105.13        70960-71083
            1181-1323           AC021105.13        79497-79639
            1324-1540           AC021105.13        103492-103708
            1541-1614           AC021105.13        110004-110077
            1615-1812           AC021105.13        110230-110427
            1813-1904           AC021105.13        111462-111553
            1905-1976           AC021105.13        112668-112739
            1977-2079           AC021105.13        113585-113687
            2080-2217           AC021105.13        113848-113985
            2218-2420           AC021105.13        118821-119023
            2421-6121           AC021105.13        121663-125363
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.22"
     gene            1..6121
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="lin-54 DREAM MuvB core complex component"
                     /db_xref="GeneID:132660"
                     /db_xref="HGNC:HGNC:25397"
                     /db_xref="MIM:613367"
     exon            1..340
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            341..1056
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..2622
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="isoform a is encoded by transcript variant 1;
                     protein lin-54 homolog; CXC domain-containing protein 1"
                     /codon_start=1
                     /product="protein lin-54 homolog isoform a"
                     /protein_id="NP_919258.2"
                     /db_xref="CCDS:CCDS3599.1"
                     /db_xref="GeneID:132660"
                     /db_xref="HGNC:HGNC:25397"
                     /db_xref="MIM:613367"
                     /translation="MEVVPAEVNSLLPEEIMDTGITLVDDDSIEAVIVSSPIPMETEL
                     EEIVNINSTGDSTATPISTEPITVYSNHTNQVAVNTTITKADSNTTVKPAFPSGLQKL
                     GAQTPVTISANQIILNKVSQTSDLKLGNQTLKPDGQKLILTTLGKSGSPIVLALPHSQ
                     LPQAQKVTTQAQSGDAKLPPQQIKVVTIGGRPEVKPVIGVSALTPGSQLINTTTQPSV
                     LQTQQLKTVQIAKKPRTPTSGPVITKLIFAKPINSKAVTGQTTQVSPPVIAGRVLSQS
                     TPGTPSKTITISESGVIGSTLNSTTQTPNKIAISPLKSPNKAVKSTVQTITVGGVSTS
                     QFKTIIPLATAPNVQQIQVPGSKFHYVRLVTATSASSSTQPVSQNPSTNTQPLQQAKP
                     VVVNTTPVRMSVPIVSAQAVKQVVPKPINPTSQIVTTSQPQQRLIMPATPLPQIQPNL
                     TNLPPGTVLAPAPGTGNVGYAVLPAQYVTQLQQSSYVSIASNSTFTGTSGIQTQARLP
                     FNGIIPSESASRPRKPCNCTKSLCLKLYCDCFANGEFCNNCNCTNCYNNLEHENERQK
                     AIKACLDRNPEAFKPKIGKGKEGESDRRHSKGCNCKRSGCLKNYCECYEAKIMCSSIC
                     KCIGCKNFEESPERKTLMHLADAAEVRVQQQTAAKTKLSSQISDLLTRPTPALNSGGG
                     KLPFTFVTKEVAEATCNCLLAQAEQADKKGKSKAAAERMILEEFGRCLMSVINSAGKA
                     KSDPCAMNC"
     exon            1057..1180
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1323
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1540
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1614
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1812
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1904
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1976
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2079
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2217
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2420
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..6121
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4981..4986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="hexamer: AATAAA"
     polyA_site      5009
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="major polyA site"
     regulatory      5735..5740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="hexamer: AATAAA"
     polyA_site      5759
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
     regulatory      6095..6100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
                     /note="hexamer: ATTAAA"
     polyA_site      6121
                     /gene="LIN54"
                     /gene_synonym="CXCDC1; JC8.6; MIP120; TCX1"
ORIGIN      
        1 actggtggtg gcggcggcgg cggcggcccg ggctccggct gtcggggaag ctgctgagct
       61 gtgatggtga tgacgaggtg aaaatggcgg atctttcgaa atacaatccc ggcccctgac
      121 ataccagagg cggcggcgac ggcgacgtcg ccaccccggc tcccttctcg gtccccgggt
      181 accctggagc gctccagttt ggacaaactg ggaaaggaag ctgctggcca gagctatcgt
      241 gcacgggcaa aacaagcccc gccggggctc gggattgccc aggaccttct ggagccccta
      301 cctcggagcc cgagggagga gaaagcggca gccgctggag tgcctgatca actttctagc
      361 gggagaacga tcatggaggt ggtgccagct gaggtgaata gtttgcttcc agaggaaata
      421 atggacactg gtataacttt agtggatgat gatagtattg aggctgttat tgtttcatcc
      481 ccaattccca tggagacaga actggaagaa attgtcaaca taaattctac tggtgactct
      541 acagccacgc ccatttccac ggaaccaatc acagtgtaca gtaaccacac taaccaagtt
      601 gcagtgaata ccacaattac taaagcagat tctaatacca cagtgaaacc agcttttcca
      661 agtggccttc aaaaacttgg tgctcagact cctgtgacta tatcagccaa tcagattatt
      721 ttaaacaaag tatcacagac atctgatctt aaacttggca atcagaccct taaaccagat
      781 ggacagaagt taattttaac aactttgggc aagtctggtt caccaattgt tttagcacta
      841 ccccatagcc aactacccca ggctcagaaa gttacaactc aggcccagtc aggagatgct
      901 aagttaccac cgcagcaaat taaagtagtt accattggag ggaggccaga ggtgaaacct
      961 gtcattggtg tctcagcatt gaccccagga agtcaactga ttaatactac aactcagccc
     1021 tctgtgttac agacccaaca gttaaaaaca gtacagattg ctaagaagcc tcgaacgcca
     1081 acctctggtc cagtaatcac gaagctgatc tttgcaaaac caattaatag taaagcagtt
     1141 acaggacaga caactcaagt ttcaccacca gttattgcag gtagggttct ttcacagtct
     1201 actcccggaa ctccatcaaa gaccataaca atatctgaaa gtggtgttat tggatcaact
     1261 ttaaattcta caacacagac accaaataaa atagccatct cacctttgaa atcgccaaat
     1321 aaggcagtga aatcaactgt gcagaccatc actgttggag gagtgagcac atcacagttt
     1381 aagacaatta ttcctctggc aactgctccc aatgtccagc agattcaagt gcctggaagc
     1441 aagtttcatt atgtccgact tgttactgcc acatcagcca gtagctcaac ccagccagtt
     1501 agtcagaatc ccagtacaaa cactcagcct cttcagcaag caaagccagt ggttgttaat
     1561 acaaccccag tgcggatgtc agttccaatt gtctcagctc aggctgtcaa acaagttgtt
     1621 ccaaaaccaa tcaatccaac ttcacaaata gtaactacta gccagccaca gcaacggctt
     1681 atcatgcctg ccacaccact gccacagatc cagcccaacc tcactaacct gccaccaggc
     1741 actgtcctgg caccagctcc gggaacaggg aatgtgggtt atgcagtgct tccagctcag
     1801 tatgttactc agctacagca gtcttcatat gtatcaatag caagcaactc tacctttact
     1861 ggaacatctg gtatccagac ccaggcacgg cttccattca atggcataat cccatcagag
     1921 tcggccagtc ggccccgaaa gccctgtaat tgtacaaaat cactgtgttt gaaattgtat
     1981 tgtgattgct ttgcaaatgg tgaattttgc aacaactgca attgtactaa ttgttacaac
     2041 aatttggaac atgaaaatga aaggcaaaaa gcaataaagg catgccttga cagaaatcca
     2101 gaagccttta agcctaagat agggaaagga aaggagggag aatctgatcg acgtcatagc
     2161 aaagggtgta attgcaaacg atcaggatgt cttaaaaact actgtgaatg ctatgaggca
     2221 aaaataatgt gttcctcaat atgcaaatgt attggctgta agaattttga agaaagcccg
     2281 gaaaggaaga cattgatgca tttggcagat gcagctgaag taagggtaca gcaacaaaca
     2341 gcagccaaga cgaagttatc ctctcaaatt tcagacttgc ttactaggcc aacaccagct
     2401 ttaaatagtg gaggcggaaa attgccattt acatttgtaa ctaaggaagt agctgaagcc
     2461 acatgtaatt gcctccttgc ccaggcagag caggcagaca agaagggaaa atcaaaggca
     2521 gcagcggaac ggatgatact tgaggaattc ggacgatgtt tgatgagtgt catcaactct
     2581 gcaggaaagg caaaaagtga cccttgtgcc atgaattgct aactcttgca caaaagactg
     2641 ataaatggaa ctgtacagaa aatttaaggt gcagggacac ttgattttct ggaagaaaaa
     2701 caattactgt attttaattc agtccttgtt ttaaaagacc tgaaattata atactgaagg
     2761 agaagaaatt ttaaatgagg aaattagtac attttaaatc ttagttaaat ctgcttatgc
     2821 cccttctaaa ttgaattttt ctttattata tagatttttt aatttgcttg ggtttcttaa
     2881 gaattagatg ttctctttct gatacctttg acaaaaaatg tttataaatt catataattt
     2941 ataatgtatg gtgttgtatg actttgttaa tagaaaagcc aaagcagcag tggttagcac
     3001 ccattctttg ggacttgatc tagaatatct gcagacagaa tgttacataa acaaattctt
     3061 atgaaacaca ttcaaatgac attttgtatt tagaaaagga ctatctttta aagaaaaagc
     3121 agccttttag ggccgattct ggaataatat cctgttgtca ctttgggaat gtcagaaggg
     3181 gaaacaatcc ccaggcacac taaaaatttt ttaaagttat ttaaaaaaac atataaaata
     3241 ttaaaggaca gtaaatctca gaggatgggc aatgtgtttc tataataagg aaaggctaac
     3301 agatgctctg ggctgtctcc attttctttc aaagaggtgg tatgtatttg aagtaataaa
     3361 ttgtcaaagt gattactggg tactattaaa atgataggtg gatataaatg gaagtaaaca
     3421 ttatgtagtg ataatataga acctcacata gtaatcaagt ataaaatttg gcatgggtgg
     3481 agaaacaaag aatagggaag ctgcaaaaga tgaatttaga gaagttttca tctatacaat
     3541 caattatttt tacagacttt tttttttttt tttttgagac ggagtctcgc tgtgtagccc
     3601 aggctggagt gcaatggtga gatctcagct cactgcaacg cctctgcctc ccaggttcaa
     3661 gcgattctcc tgcctcagcc tcccgtgtag ctgggactac aggtgcatgc caccatgccc
     3721 ggctcatttt ttgtattttt agtagagacg gggtttcacc gtgttagcca ggatggtctc
     3781 gatctcctga cctcgtgatt cgcccgcctc ggcctcccaa agtgctggga ttacaggcat
     3841 aagccgccgt gccaggccct ttttatagac ttttgaacta agaggtttca aaccaaatcg
     3901 caactcttac ttgatttaca gaaatgggcc tcctaacaaa attttcaaaa ttgtggtggg
     3961 atttcttttt ataagggaag tttaatatgg cattattttc agccagtgat ttggaaggtc
     4021 tgttttccta taattactaa aataacctaa atcattttca gtattcaaaa actctatgaa
     4081 tttaagagcc tgttgtgaca ggactcttga ttttatgaca agaaaaaacc atgttggaat
     4141 tcaggcattt tgttaggtac cttaatcatc agtaattagt atatgattat ttcatttcct
     4201 acaaggactc tatagttgta tgtattaagt gtgtatatat gttcatatga acatgcaaga
     4261 tataggcaat agaaattaag taatccaaaa gaggattccg ttattcacta aagtatcttc
     4321 cttttaaatt tggatttata aaatcatagt gcagtaactt taaatgtaga tagttcaaac
     4381 attcttagca cctcaatcta gtatgtttaa ttaaaatttg tataaatgta aattagtgta
     4441 agagggtaaa ataataatca agttattttt caaaagattg caataagttt ttggtctaaa
     4501 cttaaacttt gttcattttg tacatactct gtgttaattc taattgcacc tttgtgatgt
     4561 gtatttatat acagtgtgca gaaaatgttc gtgaaatttt gattacaatt gtagattatg
     4621 tatgatggca ttgcttacga atgttttgct tgtaaaaaaa tttcaaggtg caatcaaatg
     4681 ctactagttt ttctgatttt caaaaatcta tctatctaaa atattaagcc ttttccttcc
     4741 tgtgtagtac ttactaaata actttttgta ttcaggcatt tgcatcaaat tgctgaacaa
     4801 gatgaatagc cacattaatt caatgatttt aaaagactat ttggggaggg ttgggtatat
     4861 aattttttag ctctatttac atcccaaagt agaaagatta aatttatatt tactagagct
     4921 attcaaagaa tacacttttc tatattgtaa aaacaggaca gcaaagtaaa tcatacagaa
     4981 aataaacatt tatactattc tgtaaaaaag gttttgcatt ttttctttag tataataaca
     5041 ccactttatt aaccatttaa tatgcaaaaa accttttaaa ttatctgaag taaatttgac
     5101 attacttctg aaaacacaca actaaaaaat tctatcattt tgctcagtta ttactaacat
     5161 aaattgcttt gaaatgagaa tatgtcttct ctttttttcc ccactgtttc ttttaagagc
     5221 agaaactcag ccgggcatgg tggctcacgc ctgtaattcc agcactttgg gaggtcaagg
     5281 cgggtgaatc acttgagtcc aggagttcta gaccagccca ggctacatgg tgaaaccctg
     5341 tctctactaa aaatataaaa aaattcactg cgtgtggtgg cgcacacctg cagtcctcgc
     5401 tactttgaag gcctgagcct gcagaggttg aggctgtagt gagtcaaaat tacaccactg
     5461 cactccagcc tggcaatcag agtgagacct tgtctcaaaa acaaaaatac ctagaaaatc
     5521 aatctactct gtcttttaat gtgaaatgtt cttatgatag ctatctttct tagtttcctt
     5581 tttttctgaa gcactaaaca caacctgtag gtcttatctc tggggtctgg gaaacagaac
     5641 cttaatgtta caggtacaaa agcaaacaga gtgattagtt ccccattttc tggtagtgaa
     5701 caactgacat tttttcaatc ttatgtaaaa tgtgaataaa aataatttta gaaaagttat
     5761 ctatttttat tgttctgtaa cacaaatagt taagaaaatg aatacttgtt atgtaaatga
     5821 agcttcacag caggacctcc ggccataact ttgatcatgt tgtatctctt aagcatttta
     5881 tataggaatt ctggtgtggc tacccaaagc agaagggaag gcaatcaact aaaaatccct
     5941 ttctattgga gttattttca tcatgtaaat tacttcaggc ttttcttgtt agggcttctt
     6001 gaatgcacat tttgtcatta tctgtatacc aaaggtgagc tttttttttt aatccatgtg
     6061 attttatgag ttgaaagcta taaggttttt tttaattaaa aatttccttc taaatggcaa
     6121 a
//
