LOCUS       NM_005569               3667 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a,
            mRNA.
ACCESSION   NM_005569
VERSION     NM_005569.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3667)
  AUTHORS   Su,Y., Xu,B., Shen,Q., Lei,Z., Zhang,W. and Hu,T.
  TITLE     LIMK2 Is a Novel Prognostic Biomarker and Correlates With Tumor
            Immune Cell Infiltration in Lung Squamous Cell Carcinoma
  JOURNAL   Front Immunol 13, 788375 (2022)
   PUBMED   35273591
  REMARK    GeneRIF: LIMK2 Is a Novel Prognostic Biomarker and Correlates With
            Tumor Immune Cell Infiltration in Lung Squamous Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3667)
  AUTHORS   Li,J., Liu,Y. and Zheng,L.
  TITLE     The Diagnostic and Prognostic Value of LIMK1/2: A Pan-Cancer
            Analysis
  JOURNAL   Ann Clin Lab Sci 51 (5), 615-624 (2021)
   PUBMED   34686503
  REMARK    GeneRIF: The Diagnostic and Prognostic Value of LIMK1/2: A
            Pan-Cancer Analysis.
REFERENCE   5  (bases 1 to 3667)
  AUTHORS   He,Y., Zhang,L., He,Y., Yu,H., Li,S. and Li,Q.
  TITLE     Involvement of LIMK2 in actin cytoskeleton remodeling during the
            definitive endoderm differentiation
  JOURNAL   In Vitro Cell Dev Biol Anim 57 (5), 493-500 (2021)
   PUBMED   33977398
  REMARK    GeneRIF: Involvement of LIMK2 in actin cytoskeleton remodeling
            during the definitive endoderm differentiation.
REFERENCE   6  (bases 1 to 3667)
  AUTHORS   Nomoto,S., Tatematsu,Y., Takahashi,T. and Osada,H.
  TITLE     Cloning and characterization of the alternative promoter regions of
            the human LIMK2 gene responsible for alternative transcripts with
            tissue-specific expression
  JOURNAL   Gene 236 (2), 259-271 (1999)
   PUBMED   10452946
REFERENCE   7  (bases 1 to 3667)
  AUTHORS   Yang,N., Higuchi,O., Ohashi,K., Nagata,K., Wada,A., Kangawa,K.,
            Nishida,E. and Mizuno,K.
  TITLE     Cofilin phosphorylation by LIM-kinase 1 and its role in
            Rac-mediated actin reorganization
  JOURNAL   Nature 393 (6687), 809-812 (1998)
   PUBMED   9655398
REFERENCE   8  (bases 1 to 3667)
  AUTHORS   Osada,H., Hasada,K., Inazawa,J., Uchida,K., Ueda,R., Takahashi,T.
            and Takahashi,T.
  TITLE     Subcellular localization and protein interaction of the human LIMK2
            gene expressing alternative transcripts with tissue-specific
            regulation
  JOURNAL   Biochem Biophys Res Commun 229 (2), 582-589 (1996)
   PUBMED   8954941
REFERENCE   9  (bases 1 to 3667)
  AUTHORS   Hiraoka,J., Okano,I., Higuchi,O., Yang,N. and Mizuno,K.
  TITLE     Self-association of LIM-kinase 1 mediated by the interaction
            between an N-terminal LIM domain and a C-terminal kinase domain
  JOURNAL   FEBS Lett 399 (1-2), 117-121 (1996)
   PUBMED   8980133
REFERENCE   10 (bases 1 to 3667)
  AUTHORS   Okano,I., Hiraoka,J., Otera,H., Nunoue,K., Ohashi,K., Iwashita,S.,
            Hirai,M. and Mizuno,K.
  TITLE     Identification and characterization of a novel family of
            serine/threonine kinases containing two N-terminal LIM motifs
  JOURNAL   J Biol Chem 270 (52), 31321-31330 (1995)
   PUBMED   8537403
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX385507.2, D45906.1,
            BC013051.1 and AC002073.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005569.3.
            
            Summary: There are approximately 40 known eukaryotic LIM proteins,
            so named for the LIM domains they contain. LIM domains are highly
            conserved cysteine-rich structures containing 2 zinc fingers.
            Although zinc fingers usually function by binding to DNA or RNA,
            the LIM motif probably mediates protein-protein interactions. LIM
            kinase-1 and LIM kinase-2 belong to a small subfamily with a unique
            combination of 2 N-terminal LIM motifs and a C-terminal protein
            kinase domain. The protein encoded by this gene is phosphorylated
            and activated by ROCK, a downstream effector of Rho, and the
            encoded protein, in turn, phosphorylates cofilin, inhibiting its
            actin-depolymerizing activity. It is thought that this pathway
            contributes to Rho-induced reorganization of the actin
            cytoskeleton. At least three transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2a) differs in the 5' UTR and
            coding region as well as the 3' UTR and coding region, compared to
            variant 1. The resulting isoform (2a) is shorter and has distinct
            N- and C-termini compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D45906.1, SRR1803617.62527.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331728.9/ ENSP00000332687.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               BX385507.2         35-247
            214-1319            D45906.1           217-1322
            1320-1883           BC013051.1         1376-1939
            1884-3667           AC002073.1         26807-28590         c
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..3667
                     /gene="LIMK2"
                     /note="LIM domain kinase 2"
                     /db_xref="GeneID:3985"
                     /db_xref="HGNC:HGNC:6614"
                     /db_xref="MIM:601988"
     exon            1..127
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..2028
                     /gene="LIMK2"
                     /EC_number="2.7.11.1"
                     /note="isoform 2a is encoded by transcript variant 2a"
                     /codon_start=1
                     /product="LIM domain kinase 2 isoform 2a"
                     /protein_id="NP_005560.1"
                     /db_xref="CCDS:CCDS13891.1"
                     /db_xref="GeneID:3985"
                     /db_xref="HGNC:HGNC:6614"
                     /db_xref="MIM:601988"
                     /translation="MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQ
                     DSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCK
                     VIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTE
                     GRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVE
                     EVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLE
                     GTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHG
                     EVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFI
                     GVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHR
                     DLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVG
                     NPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEK
                     FVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDH
                     TVSMQYGLTRDSPP"
     exon            128..227
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            228..363
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            364..473
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            474..662
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            663..768
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            769..965
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1152
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1239
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1371
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1428
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1494
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1669
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1725
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1883
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..3667
                     /gene="LIMK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3292..3297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIMK2"
                     /note="hexamer: AATAAA"
     polyA_site      3321
                     /gene="LIMK2"
     regulatory      3638..3643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIMK2"
                     /note="hexamer: AATAAA"
     polyA_site      3667
                     /gene="LIMK2"
                     /note="major polyA site"
ORIGIN      
        1 gtcttcccgc gcctgaggcg gcggcggcag gagctgaggg gagttgtagg gaactgaggg
       61 gagctgctgt gtcccccgcc tcctcctccc catttccgcg ctcccgggac catgtccgcg
      121 ctggcgggtg aagatgtctg gaggtgtcca ggctgtgggg accacattgc tccaagccag
      181 atatggtaca ggactgtcaa cgaaacctgg cacggctctt gcttccggtg ttcagaatgc
      241 caggattccc tcaccaactg gtactatgag aaggatggga agctctactg ccccaaggac
      301 tactggggga agtttgggga gttctgtcat gggtgctccc tgctgatgac agggcctttt
      361 atggtggctg gggagttcaa gtaccaccca gagtgctttg cctgtatgag ctgcaaggtg
      421 atcattgagg atggggatgc atatgcactg gtgcagcatg ccaccctcta ctgtgggaag
      481 tgccacaatg aggtggtgct ggcacccatg tttgagagac tctccacaga gtctgttcag
      541 gagcagctgc cctactctgt cacgctcatc tccatgccgg ccaccactga aggcaggcgg
      601 ggcttctccg tgtccgtgga gagtgcctgc tccaactacg ccaccactgt gcaagtgaaa
      661 gaggtcaacc ggatgcacat cagtcccaac aatcgaaacg ccatccaccc tggggaccgc
      721 atcctggaga tcaatgggac ccccgtccgc acacttcgag tggaggaggt ggaggatgca
      781 attagccaga cgagccagac acttcagctg ttgattgaac atgaccccgt ctcccaacgc
      841 ctggaccagc tgcggctgga ggcccggctc gctcctcaca tgcagaatgc cggacacccc
      901 cacgccctca gcaccctgga caccaaggag aatctggagg ggacactgag gagacgttcc
      961 ctaaggcgca gtaacagtat ctccaagtcc cctggcccca gctccccaaa ggagcccctg
     1021 ctgttcagcc gtgacatcag ccgctcagaa tcccttcgtt gttccagcag ctattcacag
     1081 cagatcttcc ggccctgtga cctaatccat ggggaggtcc tggggaaggg cttctttggg
     1141 caggctatca aggtgacaca caaagccacg ggcaaagtga tggtcatgaa agagttaatt
     1201 cgatgtgatg aggagaccca gaaaactttt ctgactgagg tgaaagtgat gcgcagcctg
     1261 gaccacccca atgtgctcaa gttcattggt gtgctgtaca aggataagaa gctgaacctc
     1321 ctgacagagt acattgaggg gggcacactg aaggactttc tgcgcagtat ggatccgttc
     1381 ccctggcagc agaaggtcag gtttgccaaa ggaatcgcct ccggaatggc ctatttgcac
     1441 tctatgtgca tcatccaccg ggatctgaac tcgcacaact gcctcatcaa gttggacaag
     1501 actgtggtgg tggcagactt tgggctgtca cggctcatag tggaagagag gaaaagggcc
     1561 cccatggaga aggccaccac caagaaacgc accttgcgca agaacgaccg caagaagcgc
     1621 tacacggtgg tgggaaaccc ctactggatg gcccctgaga tgctgaacgg aaagagctat
     1681 gatgagacgg tggatatctt ctcctttggg atcgttctct gtgagatcat tgggcaggtg
     1741 tatgcagatc ctgactgcct tccccgaaca ctggactttg gcctcaacgt gaagcttttc
     1801 tgggagaagt ttgttcccac agattgtccc ccggccttct tcccgctggc cgccatctgc
     1861 tgcagactgg agcctgagag cagaccagca ttctcgaaat tggaggactc ctttgaggcc
     1921 ctctccctgt acctggggga gctgggcatc ccgctgcctg cagagctgga ggagttggac
     1981 cacactgtga gcatgcagta cggcctgacc cgggactcac ctccctagcc ctggcccagc
     2041 cccctgcagg ggggtgttct acagccagca ttgcccctct gtgccccatt cctgctgtga
     2101 gcagggccgt ccgggcttcc tgtggattgg cggaatgttt agaagcagaa caagccattc
     2161 ctattacctc cccaggaggc aagtgggcgc agcaccaggg aaatgtatct ccacaggttc
     2221 tggggcctag ttactgtctg taaatccaat acttgcctga aagctgtgaa gaagaaaaaa
     2281 acccctggcc tttgggccag gaggaatctg ttactcgaat ccacccagga actccctggc
     2341 agtggattgt gggaggctct tgcttacact aatcagcgtg acctggacct gctgggcagg
     2401 atcccagggt gaacctgcct gtgaactctg aagtcactag tccagctggg tgcaggagga
     2461 cttcaagtgt gtggacgaaa gaaagactga tggctcaaag ggtgtgaaaa agtcagtgat
     2521 gctccccctt tctactccag atcctgtcct tcctggagca aggttgaggg agtaggtttt
     2581 gaagagtccc ttaatatgtg gtggaacagg ccaggagtta gagaaagggc tggcttctgt
     2641 ttacctgctc actggctcta gccagcccag ggaccacatc aatgtgagag gaagcctcca
     2701 cctcatgttt tcaaacttaa tactggagac tggctgagaa cttacggaca acatcctttc
     2761 tgtctgaaac aaacagtcac aagcaaagga agaggctggg ggactagaaa gaggccctgc
     2821 cctctagaaa gctcagatct tggcttctgt tactcatact cgggtgggct ccttagtcag
     2881 atgcctaaaa cattttgcct aaagctcgat gggttctgga ggacagtgtg gcttgtcaca
     2941 ggcctagagt ctgagggagg ggagtgggag tctcagcaat ctcttggtct tggcttcatg
     3001 gcaaccactg ctcacccttc aacatgcctg gtttaggcag cagcttgggc tgggaagagg
     3061 tggtggcaga gtctcaaagc tgagatgctg agagagatag ctccctgagc tgggccatct
     3121 gacttctacc tcccatgttt gctctcccaa ctcattagct cctgggcagc atcctcctga
     3181 gccacatgtg caggtactgg aaaacctcca tcttggctcc cagagctcta ggaactcttc
     3241 atcacaacta gatttgcctc ttctaagtgt ctatgagctt gcaccatatt taataaattg
     3301 ggaatgggtt tggggtatta atgcaatgtg tggtggttgt attggagcag ggggaattga
     3361 taaaggagag tggttgctgt taatattatc ttatctattg ggtggtatgt gaaatattgt
     3421 acatagacct gatgagttgt gggaccagat gtcatctctg gtcagagttt acttgctata
     3481 tagactgtac ttatgtgtga agtttgcaag cttgctttag ggctgagccc tggactccca
     3541 gcagcagcac agttcagcat tgtgtggctg gttgtttcct ggctgtcccc agcaagtgta
     3601 ggagtggtgg gcctgaactg ggccattgat cagactaaat aaattaagca gttaacataa
     3661 ctggcaa
//
