LOCUS       NM_002314               3355 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1,
            mRNA.
ACCESSION   NM_002314
VERSION     NM_002314.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Kippenhan,J.S., Gregory,M.D., Nash,T., Kohn,P., Mervis,C.B.,
            Eisenberg,D.P., Garvey,M.H., Roe,K., Morris,C.A., Kolachana,B.,
            Pani,A.M., Sorcher,L. and Berman,K.F.
  TITLE     Dorsal visual stream and LIMK1: hemideletion, haplotype, and
            enduring effects in children with Williams syndrome
  JOURNAL   J Neurodev Disord 15 (1), 29 (2023)
   PUBMED   37633900
  REMARK    GeneRIF: Dorsal visual stream and LIMK1: hemideletion, haplotype,
            and enduring effects in children with Williams syndrome.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Noh,J.M., Choi,S.C., Song,M.H., Kim,K.S., Jun,S., Park,J.H.,
            Kim,J.H., Kim,K., Ko,T.H., Choi,J.I., Gim,J.A., Kim,J.H., Jang,Y.,
            Park,Y., Na,J.E., Rhyu,I.J. and Lim,D.S.
  TITLE     The Activation of the LIMK/Cofilin Signaling Pathway via
            Extracellular Matrix-Integrin Interactions Is Critical for the
            Generation of Mature and Vascularized Cardiac Organoids
  JOURNAL   Cells 12 (16), 2029 (2023)
   PUBMED   37626839
  REMARK    GeneRIF: The Activation of the LIMK/Cofilin Signaling Pathway via
            Extracellular Matrix-Integrin Interactions Is Critical for the
            Generation of Mature and Vascularized Cardiac Organoids.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3355)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 3355)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3355)
  AUTHORS   Li,J., Liu,Y. and Zheng,L.
  TITLE     The Diagnostic and Prognostic Value of LIMK1/2: A Pan-Cancer
            Analysis
  JOURNAL   Ann Clin Lab Sci 51 (5), 615-624 (2021)
   PUBMED   34686503
  REMARK    GeneRIF: The Diagnostic and Prognostic Value of LIMK1/2: A
            Pan-Cancer Analysis.
REFERENCE   6  (bases 1 to 3355)
  AUTHORS   Osborne,L.R., Martindale,D., Scherer,S.W., Shi,X.M., Huizenga,J.,
            Heng,H.H., Costa,T., Pober,B., Lew,L., Brinkman,J., Rommens,J.,
            Koop,B. and Tsui,L.C.
  TITLE     Identification of genes from a 500-kb region at 7q11.23 that is
            commonly deleted in Williams syndrome patients
  JOURNAL   Genomics 36 (2), 328-336 (1996)
   PUBMED   8812460
REFERENCE   7  (bases 1 to 3355)
  AUTHORS   Frangiskakis,J.M., Ewart,A.K., Morris,C.A., Mervis,C.B.,
            Bertrand,J., Robinson,B.F., Klein,B.P., Ensing,G.J., Everett,L.A.,
            Green,E.D., Proschel,C., Gutowski,N.J., Noble,M., Atkinson,D.L.,
            Odelberg,S.J. and Keating,M.T.
  TITLE     LIM-kinase1 hemizygosity implicated in impaired visuospatial
            constructive cognition
  JOURNAL   Cell 86 (1), 59-69 (1996)
   PUBMED   8689688
REFERENCE   8  (bases 1 to 3355)
  AUTHORS   Tassabehji,M., Metcalfe,K., Fergusson,W.D., Carette,M.J.,
            Dore,J.K., Donnai,D., Read,A.P., Proschel,C., Gutowski,N.J., Mao,X.
            and Sheer,D.
  TITLE     LIM-kinase deleted in Williams syndrome
  JOURNAL   Nat Genet 13 (3), 272-273 (1996)
   PUBMED   8673124
REFERENCE   9  (bases 1 to 3355)
  AUTHORS   Okano,I., Hiraoka,J., Otera,H., Nunoue,K., Ohashi,K., Iwashita,S.,
            Hirai,M. and Mizuno,K.
  TITLE     Identification and characterization of a novel family of
            serine/threonine kinases containing two N-terminal LIM motifs
  JOURNAL   J Biol Chem 270 (52), 31321-31330 (1995)
   PUBMED   8537403
REFERENCE   10 (bases 1 to 3355)
  AUTHORS   Mizuno,K., Okano,I., Ohashi,K., Nunoue,K., Kuma,K., Miyata,T. and
            Nakamura,T.
  TITLE     Identification of a human cDNA encoding a novel protein kinase with
            two repeats of the LIM/double zinc finger motif
  JOURNAL   Oncogene 9 (6), 1605-1612 (1994)
   PUBMED   8183554
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005056.2, D26309.1 and
            AW016257.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002314.3.
            
            Summary: There are approximately 40 known eukaryotic LIM proteins,
            so named for the LIM domains they contain. LIM domains are highly
            conserved cysteine-rich structures containing 2 zinc fingers.
            Although zinc fingers usually function by binding to DNA or RNA,
            the LIM motif probably mediates protein-protein interactions. LIM
            kinase-1 and LIM kinase-2 belong to a small subfamily with a unique
            combination of 2 N-terminal LIM motifs and a C-terminal protein
            kinase domain. LIMK1 is a serine/threonine kinase that regulates
            actin polymerization via phosphorylation and inactivation of the
            actin binding factor cofilin. This protein is ubiquitously
            expressed during development and plays a role in many cellular
            processes associated with cytoskeletal structure. This protein also
            stimulates axon growth and may play a role in brain development.
            LIMK1 hemizygosity is implicated in the impaired visuospatial
            constructive cognition of Williams syndrome. Alternative splicing
            results in multiple transcript variants encoding distinct
            isoforms.[provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments. The exon
            structure of the 5' UTR has been inferred from cross-species
            comparisons and lacks direct experimental support.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.8350.1,
                                           SRR1660805.38201.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336180.7/ ENSP00000336740.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC005056.2         10205-10338         c
            135-3354            D26309.1           43-3262
            3355-3355           AW016257.1         17-17               c
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.23"
     gene            1..3355
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /note="LIM domain kinase 1"
                     /db_xref="GeneID:3984"
                     /db_xref="HGNC:HGNC:6613"
                     /db_xref="MIM:601329"
     exon            1..242
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..2131
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; LIM
                     motif-containing protein kinase"
                     /codon_start=1
                     /product="LIM domain kinase 1 isoform 1"
                     /protein_id="NP_002305.1"
                     /db_xref="CCDS:CCDS5563.1"
                     /db_xref="GeneID:3984"
                     /db_xref="HGNC:HGNC:6613"
                     /db_xref="MIM:601329"
                     /translation="MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNAD
                     WHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGEL
                     KYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLP
                     HTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIH
                     VGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSP
                     AYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIF
                     RPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLE
                     HPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAY
                     LHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYT
                     VVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGF
                     LDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQL
                     DRGFWETYRRGESGLPAHPEVPD"
     exon            243..339
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            340..478
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            479..588
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            589..795
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            796..901
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1068
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1252
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1339
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1471
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1531
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1597
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1754
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1810
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1968
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..3355
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3332..3337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /note="hexamer: AATAAA"
     polyA_site      3355
                     /gene="LIMK1"
                     /gene_synonym="LIMK; LIMK-1"
                     /note="major polyA site"
ORIGIN      
        1 gctcgctccc caagccgccg cggcgccgag ccggtttccc cgccggtgtc cgagaggcgc
       61 ccccggcccg gcccggcccg gcccgcgccc tccgcccccg cctccccggg ccggcggcgg
      121 tgggcgagct cgcgggcccg gccgccccca gccccagccc cgccgggccc cgccccccgt
      181 cgagtgcatg aggttgacgc tactttgttg cacctggagg gaagaacgta tgggagagga
      241 aggaagcgag ttgcccgtgt gtgcaagctg cggccagagg atctatgatg gccagtacct
      301 ccaggccctg aacgcggact ggcacgcaga ctgcttcagg tgttgtgact gcagtgcctc
      361 cctgtcgcac cagtactatg agaaggatgg gcagctcttc tgcaagaagg actactgggc
      421 ccgctatggc gagtcctgcc atgggtgctc tgagcaaatc accaagggac tggttatggt
      481 ggctggggag ctgaagtacc accccgagtg tttcatctgc ctcacgtgtg ggacctttat
      541 cggtgacggg gacacctaca cgctggtgga gcactccaag ctgtactgcg ggcactgcta
      601 ctaccagact gtggtgaccc ccgtcatcga gcagatcctg cctgactccc ctggctccca
      661 cctgccccac accgtcaccc tggtgtccat cccagcctca tctcatggca agcgtggact
      721 ttcagtctcc attgaccccc cgcacggccc accgggctgt ggcaccgagc actcacacac
      781 cgtccgcgtc cagggagtgg atccgggctg catgagccca gatgtgaaga attccatcca
      841 cgtcggagac cggatcttgg aaatcaatgg cacgcccatc cgaaatgtgc ccctggacga
      901 gattgacctg ctgattcagg aaaccagccg cctgctccag ctgaccctcg agcatgaccc
      961 tcacgataca ctgggccacg ggctggggcc tgagaccagc cccctgagct ctccggctta
     1021 tactcccagc ggggaggcgg gcagctctgc ccggcagaaa cctgtcttga ggagctgcag
     1081 catcgacagg tctccgggcg ctggctcact gggctccccg gcctcccagc gcaaggacct
     1141 gggtcgctct gagtccctcc gcgtagtctg ccggccacac cgcatcttcc ggccgtcgga
     1201 cctcatccac ggggaggtgc tgggcaaggg ctgcttcggc caggctatca aggtgacaca
     1261 ccgtgagaca ggtgaggtga tggtgatgaa ggagctgatc cggttcgacg aggagaccca
     1321 gaggacgttc ctcaaggagg tgaaggtcat gcgatgcctg gaacacccca acgtgctcaa
     1381 gttcatcggg gtgctctaca aggacaagag gctcaacttc atcactgagt acatcaaggg
     1441 cggcacgctc cggggcatca tcaagagcat ggacagccag tacccatgga gccagagagt
     1501 gagctttgcc aaggacatcg catcagggat ggcctacctc cactccatga acatcatcca
     1561 ccgagacctc aactcccaca actgcctggt ccgcgagaac aagaatgtgg tggtggctga
     1621 cttcgggctg gcgcgtctca tggtggacga gaagactcag cctgagggcc tgcggagcct
     1681 caagaagcca gaccgcaaga agcgctacac cgtggtgggc aacccctact ggatggcacc
     1741 tgagatgatc aacggccgca gctatgatga gaaggtggat gtgttctcct ttgggatcgt
     1801 cctgtgcgag atcatcgggc gggtgaacgc agaccctgac tacctgcccc gcaccatgga
     1861 ctttggcctc aacgtgcgag gattcctgga ccgctactgc cccccaaact gccccccgag
     1921 cttcttcccc atcaccgtgc gctgttgcga tctggacccc gagaagaggc catcctttgt
     1981 gaagctggaa cactggctgg agaccctccg catgcacctg gccggccacc tgccactggg
     2041 cccacagctg gagcagctgg acagaggttt ctgggagacc taccggcgcg gcgagagcgg
     2101 actgcctgcc caccctgagg tccccgactg agccagggcc actcagctgc ccctgtcccc
     2161 acctctggag aatccacccc caccagattc ctccgcggga ggtggccctc agctgggaca
     2221 gtggggaccc aggcttctcc tcagagccag gccctgactt gccttctccc accccgtgga
     2281 ccgcttcccc tgccttctct ctgccgtggc ccagagccgg cccagctgca cacacacacc
     2341 atgctctcgc cctgctgtaa cctctgtctt ggcagggctg tcccctcttg cttctccttg
     2401 catgagctgg agggcctgtg tgagttacgc ccctttccac acgccgctgc cccagcaacc
     2461 ctgttcacgc tccacctgtc tggtccatag ctccctggag gctgggccag gaggcagcct
     2521 ccgaaccatg ccccatataa cgcttgggtg cgtgggaggg cgcacatcag ggcagaggcc
     2581 aagttccagg tgtctgtgtt cccaggaacc aaatggggag tctggggccc gttttccccc
     2641 cagggggtgt ctaggtagca acaggtatcg aggactctcc aaacccccaa agcagagaga
     2701 gggctgatcc catggggcgg aggtccccag tggctgagca aacagcccct tctctcgctt
     2761 tgggtctttt ttttgtttct ttcttaaagc cactttagtg agaagcaggt accaagcctc
     2821 agggtgaagg gggtcccttg agggagcgtg gagctgcggt gccctggccg gcgatgggga
     2881 ggagccggct ccggcagtga gaggataggc acagtggacc gggcaggtgt ccaccagcag
     2941 ctcagcccct gcagtcatct cagagcccct tcccgggcct ctcccccaag gctccctgcc
     3001 cctcctcatg cccctctgtc ctctgcgttt tttctgtgta atctattttt taagaagagt
     3061 ttgtattatt ttttcatacg gctgcagcag cagctgccag gggcttggga ttttattttt
     3121 gtggcgggcg ggggtgggag ggccattttg tcactttgcc tcagttgagc atctaggaag
     3181 tattaaaact gtgaagcttt ctcagtgcac tttgaacctg gaaaacaatc ccaacaggcc
     3241 cgtgggacca tgacttaggg aggtgggacc cacccacccc catccaggaa ccgtgacgtc
     3301 caaggaacca aacccagacg cagaacaata aaataaattc cgtactcccc accca
//
