LOCUS       NM_016357               3680 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript
            variant 2, mRNA.
ACCESSION   NM_016357
VERSION     NM_016357.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Obulkasim,H., Adili,A., Liu,Y. and Duan,S.
  TITLE     Expression and molecular insights of lima1 in cholangiocarcinoma
  JOURNAL   Cell Adh Migr 18 (1), 4-17 (2024)
   PUBMED   39076043
  REMARK    GeneRIF: Expression and molecular insights of lima1 in
            cholangiocarcinoma.
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Hecht,M., Alber,N., Marhoffer,P., Johnsson,N. and Gronemeyer,T.
  TITLE     The concerted action of SEPT9 and EPLIN modulates the adhesion and
            migration of human fibroblasts
  JOURNAL   Life Sci Alliance 7 (7), e202201686 (2024)
   PUBMED   38719752
  REMARK    GeneRIF: The concerted action of SEPT9 and EPLIN modulates the
            adhesion and migration of human fibroblasts.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3680)
  AUTHORS   Liu,Z., Yang,L.Y., Hao,J.J., Zhang,N., Fan,Z.L., Cai,H.Q., Cai,Y.,
            Wei,W.Q., Zhang,Y. and Wang,M.R.
  TITLE     Nuclear-cytoplasmic translocation of SQSTM1/p62 protein enhances
            ESCC cell migration and invasion by stabilizing EPLIN expression
  JOURNAL   Exp Cell Res 435 (1), 113910 (2024)
   PUBMED   38185251
  REMARK    GeneRIF: Nuclear-cytoplasmic translocation of SQSTM1/p62 protein
            enhances ESCC cell migration and invasion by stabilizing EPLIN
            expression.
REFERENCE   4  (bases 1 to 3680)
  AUTHORS   Jiang,W., Yang,X., Shi,K., Zhang,Y., Shi,X., Wang,J., Wang,Y.,
            Chenyan,A., Shan,J., Wang,Y., Chang,J., Chen,R., Zhou,T., Zhu,Y.,
            Yu,Y., Li,C. and Li,X.
  TITLE     MAD2 activates IGF1R/PI3K/AKT pathway and promotes
            cholangiocarcinoma progression by interfering USP44/LIMA1 complex
  JOURNAL   Oncogene 42 (45), 3344-3357 (2023)
   PUBMED   37752233
  REMARK    GeneRIF: MAD2 activates IGF1R/PI3K/AKT pathway and promotes
            cholangiocarcinoma progression by interfering USP44/LIMA1 complex.
REFERENCE   5  (bases 1 to 3680)
  AUTHORS   Li,D., Neo,S.P., Gunaratne,J. and Sabapathy,K.
  TITLE     EPLIN-beta is a novel substrate of ornithine decarboxylase antizyme
            1 and mediates cellular migration
  JOURNAL   J Cell Sci 136 (12) (2023)
   PUBMED   37325974
  REMARK    GeneRIF: EPLIN-beta is a novel substrate of ornithine decarboxylase
            antizyme 1 and mediates cellular migration.
REFERENCE   6  (bases 1 to 3680)
  AUTHORS   Maul,R.S., Song,Y., Amann,K.J., Gerbin,S.C., Pollard,T.D. and
            Chang,D.D.
  TITLE     EPLIN regulates actin dynamics by cross-linking and stabilizing
            filaments
  JOURNAL   J Cell Biol 160 (3), 399-407 (2003)
   PUBMED   12566430
REFERENCE   7  (bases 1 to 3680)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 3680)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   9  (bases 1 to 3680)
  AUTHORS   Chen,S., Maul,R.S., Kim,H.R. and Chang,D.D.
  TITLE     Characterization of the human EPLIN (Epithelial Protein Lost in
            Neoplasm) gene reveals distinct promoters for the two EPLIN
            isoforms
  JOURNAL   Gene 248 (1-2), 69-76 (2000)
   PUBMED   10806352
REFERENCE   10 (bases 1 to 3680)
  AUTHORS   Maul,R.S. and Chang,D.D.
  TITLE     EPLIN, epithelial protein lost in neoplasm
  JOURNAL   Oncogene 18 (54), 7838-7841 (1999)
   PUBMED   10618726
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP281423.1, BC110815.1 and
            AL136911.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_016357.4.
            
            Summary: This gene encodes a cytoskeleton-associated protein that
            inhibits actin filament depolymerization and cross-links filaments
            in bundles. It is downregulated in some cancer cell lines.
            Alternatively spliced transcript variants encoding different
            isoforms have been described for this gene, and expression of some
            of the variants maybe independently regulated. [provided by RefSeq,
            Aug 2011].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            acceptor splice site at an internal coding exon compared to variant
            1. This results in an isoform (2, also known as EPLIN-beta) that is
            1 aa shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110815.1, AL136911.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341247.9/ ENSP00000340184.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                BP281423.1         31-43
            14-3123             BC110815.1         1-3110
            3124-3680           AL136911.1         3093-3649
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.12"
     gene            1..3680
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /note="LIM domain and actin binding 1"
                     /db_xref="GeneID:51474"
                     /db_xref="HGNC:HGNC:24636"
                     /db_xref="MIM:608364"
     exon            1..101
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            102..243
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..2404
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sterol regulatory element binding protein 3; epithelial
                     protein lost in neoplasm beta"
                     /codon_start=1
                     /product="LIM domain and actin-binding protein 1 isoform
                     2"
                     /protein_id="NP_057441.1"
                     /db_xref="CCDS:CCDS8802.1"
                     /db_xref="GeneID:51474"
                     /db_xref="HGNC:HGNC:24636"
                     /db_xref="MIM:608364"
                     /translation="MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKA
                     AEETNMEKKRSNTENLSQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRAD
                     HPPAEVTSHAASGAKADQEEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKK
                     MENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRS
                     ASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQA
                     AVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAV
                     RSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKKFQAPARETCVE
                     CQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKG
                     NYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASM
                     EAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPE
                     VPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEE
                     SVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVEN
                     GADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKEL
                     SVEEQIKRNRYYDEDEDEE"
     exon            244..289
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            290..754
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            755..839
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            840..988
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1096
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1154
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1264
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1398
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..3680
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3655..3660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /note="hexamer: AATAAA"
     polyA_site      3680
                     /gene="LIMA1"
                     /gene_synonym="EPLIN; LDLCQ8; SREBP3"
                     /note="major polyA site"
ORIGIN      
        1 agtaggtgtt agcagcttgg tcgcgacagg tgcgctaggt agagcgccgg gacctgtgac
       61 agggctggta gcagcgcaga ggaaaggcgg cttttagcca ggtatttcag tgtctgtaga
      121 caagatggaa tcatctccat ttaatagacg gcaatggacc tcactatcat tgagggtaac
      181 agccaaagaa ctttctcttg tcaacaagaa caagtcatcg gctattgtgg aaatattctc
      241 caagtaccag aaagcagctg aagaaacaaa catggagaag aagagaagta acaccgaaaa
      301 tctctcccag cactttagaa aggggaccct gactgtgtta aagaagaagt gggagaaccc
      361 agggctggga gcagagtctc acacagactc tctacggaac agcagcactg agattaggca
      421 cagagcagac catcctcctg ctgaagtgac aagccacgct gcttctggag ccaaagctga
      481 ccaagaagaa caaatccacc ccagatctag actcaggtca cctcctgaag ccctcgttca
      541 gggtcgatat ccccacatca aggacggtga ggatcttaaa gaccactcaa cagaaagtaa
      601 aaaaatggaa aattgtctag gagaatccag gcatgaagta gaaaaatcag aaatcagtga
      661 aaacacagat gcttcgggca aaatagagaa atataatgtt ccgctgaaca ggcttaagat
      721 gatgtttgag aaaggtgaac caactcaaac taagattctc cgggcccaaa gccgaagtgc
      781 aagtggaagg aagatctctg aaaacagcta ttctctagat gacctggaaa taggcccagg
      841 tcagttgtca tcttctacat ttgactcgga gaaaaatgag agtagacgaa atctggaact
      901 tccacgcctc tcagaaacct ctataaagga tcgaatggcc aagtaccagg cagctgtgtc
      961 caaacaaagc agctcaacca actatacaaa tgagctgaaa gccagtggtg gcgaaatcaa
     1021 aattcataaa atggagcaaa aggagaatgt gcccccaggt cctgaggtct gcatcaccca
     1081 tcaggaaggg gaaaagattt ctgcaaatga gaatagcctg gcagtccgtt ccacccctgc
     1141 cgaagatgac tcccgtgact cccaggttaa gagtgaggtt caacagcctg tccatcccaa
     1201 gccactaagt ccagattcca gagcctccag tctttctgaa agttctcctc ccaaagcaat
     1261 gaagaagttt caggcacctg caagagagac ctgcgtggaa tgtcagaaga cagtctatcc
     1321 aatggagcgt ctcttggcca accagcaggt gtttcacatc agctgcttcc gttgctccta
     1381 ttgcaacaac aaactcagtc taggaacata tgcatcttta catggaagaa tctattgtaa
     1441 gcctcacttc aatcaactct ttaaatctaa gggcaactat gatgaaggct ttgggcacag
     1501 accacacaag gatctatggg caagcaaaaa tgaaaacgaa gagattttgg agagaccagc
     1561 ccagcttgca aatgcaaggg agacccctca cagcccaggg gtagaagatg cccctattgc
     1621 taaggtgggt gtcctggctg caagtatgga agccaaggcc tcctctcagc aggagaagga
     1681 agacaagcca gctgaaacca agaagctgag gatcgcctgg ccacccccca ctgaacttgg
     1741 aagttcagga agtgccttgg aggaagggat caaaatgtca aagcccaaat ggcctcctga
     1801 agacgaaatc agcaagcccg aagttcctga ggatgtcgat ctagatctga agaagctaag
     1861 acgatcttct tcactgaagg aaagaagccg cccattcact gtagcagctt catttcaaag
     1921 cacctctgtc aagagcccaa aaactgtgtc cccacctatc aggaaaggct ggagcatgtc
     1981 agagcagagt gaagagtctg tgggtggaag agttgcagaa aggaaacaag tggaaaatgc
     2041 caaggcttct aagaagaatg ggaatgtggg aaaaacaacc tggcaaaaca aagaatctaa
     2101 aggagagaca gggaagagaa gtaaggaagg tcatagtttg gagatggaga atgagaatct
     2161 tgtagaaaat ggtgcagact ccgatgaaga tgataacagc ttcctcaaac aacaatctcc
     2221 acaagaaccc aagtctctga attggtcgag ttttgtagac aacacctttg ctgaagaatt
     2281 cactactcag aatcagaaat cccaggatgt ggaactctgg gagggagaag tggtcaaaga
     2341 gctctctgtg gaagaacaga taaagagaaa tcggtattat gatgaggatg aggatgaaga
     2401 gtgacaaatt gcaatgatgc tgggccttaa attcatgtta gtgttagcga gccactgccc
     2461 tttgtcaaaa tgtgatgcac ataagcaggt atcccagcat gaaatgtaat ttacttggaa
     2521 gtaactttgg aaaagaattc cttcttaaaa tcaaaaacaa aacaaaaaaa cacaaaaaac
     2581 acattctaaa tactagagat aactttactt aaattcttca ttttagcagt gatgatatgc
     2641 ataagtgctg taaggcttgt aactggggaa atattccacc tgataatagc ccagattcta
     2701 ctgtattccc aaaaggcaat attaaggtag acagatgatt agtagtatat tgttacacac
     2761 tattttggaa ttagagaaca tacagaagga atttaggggc ttaaacatta cgactgaatg
     2821 cactttagta taaagggcac agtttgtata tttttaaatg aataccaatt taatttttta
     2881 gtatttacct gttaagagat tatttagtct ttaaattttt taggttaatt ttcttgctgt
     2941 gatatatatg aggaatttac tactttatgt cctgctctct aaactacatc ctgaactcga
     3001 cgtcctgagg tataatacaa cagagcactt tttgaggcaa ttgaaaaacc aacctacact
     3061 cttcggtgct tagagagatc tgctgtctcc caaataagct tttgtatctg ccagtgaatt
     3121 tactgtactc caaatgattg ctttcttttc tggtgatatc tgtgcttctc ataattactg
     3181 aaagctgcaa tattttagta ataccttcgg gatcactgtc ccccatcttc cgtgttagag
     3241 caaagtgaag agtttaaagg aggaagaaga aagaactgtc ttacaccact tgagctcaga
     3301 cctctaaacc ctgtatttcc cttatgatgt cccctttttg agacactaat ttttaaatac
     3361 ttactagctc tgaaatatat tgatttttat cacagtattc tcagggtgaa attaaaccaa
     3421 ctataggcct ttttcttggg atgattttct agtcttaagg tttggggaca ttataaactt
     3481 gagtacattt gttgtacaca gttgatattc caaattgtat ggatgggagg gagaggtgtc
     3541 ttaagctgta ggcttttctt tgtactgcat ttatagagat ttagctttaa tattttttag
     3601 agatgtaaaa cattctgctt tcttagtctt acctagtctg aaacattttt attcaataaa
     3661 gattttaatt aaaatttgaa
//
