LOCUS       NM_013975               8382 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.
ACCESSION   NM_013975
VERSION     NM_013975.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Deng,F., Laasik,M., Salminen,L., Lapatto,L., Huhtinen,K., Li,Y.,
            Hautaniemi,S., Hynninen,J., Niemi,M. and Lehtonen,R.
  TITLE     Toxicity and therapy outcome associations in LIG3, SLCO1B3, ABCB1,
            OPRM1 and GSTP1 in high-grade serous ovarian cancer
  JOURNAL   Basic Clin Pharmacol Toxicol 132 (6), 521-531 (2023)
   PUBMED   36988399
  REMARK    GeneRIF: Toxicity and therapy outcome associations in LIG3,
            SLCO1B3, ABCB1, OPRM1 and GSTP1 in high-grade serous ovarian
            cancer.
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   McNally,J.R., Ames,A.M., Admiraal,S.J. and O'Brien,P.J.
  TITLE     Human DNA ligases I and III have stand-alone end-joining
            capability, but differ in ligation efficiency and specificity
  JOURNAL   Nucleic Acids Res 51 (2), 796-805 (2023)
   PUBMED   36625284
  REMARK    GeneRIF: Human DNA ligases I and III have stand-alone end-joining
            capability, but differ in ligation efficiency and specificity.
REFERENCE   3  (bases 1 to 8382)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 8382)
  AUTHORS   Rashid,I., Hammel,M., Sverzhinsky,A., Tsai,M.S., Pascal,J.M.,
            Tainer,J.A. and Tomkinson,A.E.
  TITLE     Direct interaction of DNA repair protein tyrosyl DNA
            phosphodiesterase 1 and the DNA ligase III catalytic domain is
            regulated by phosphorylation of its flexible N-terminus
  JOURNAL   J Biol Chem 297 (2), 100921 (2021)
   PUBMED   34181949
  REMARK    GeneRIF: Direct interaction of DNA repair protein tyrosyl DNA
            phosphodiesterase 1 and the DNA ligase III catalytic domain is
            regulated by phosphorylation of its flexible N-terminus.
REFERENCE   5  (bases 1 to 8382)
  AUTHORS   Bian,R., Dang,W., Song,X., Liu,L., Jiang,C., Yang,Y., Li,Y., Li,L.,
            Li,X., Hu,Y., Bao,R. and Liu,Y.
  TITLE     Rac GTPase activating protein 1 promotes gallbladder cancer via
            binding DNA ligase 3 to reduce apoptosis
  JOURNAL   Int J Biol Sci 17 (9), 2167-2180 (2021)
   PUBMED   34239347
  REMARK    GeneRIF: Rac GTPase activating protein 1 promotes gallbladder
            cancer via binding DNA ligase 3 to reduce apoptosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8382)
  AUTHORS   Mackey,Z.B., Ramos,W., Levin,D.S., Walter,C.A., McCarrey,J.R. and
            Tomkinson,A.E.
  TITLE     An alternative splicing event which occurs in mouse pachytene
            spermatocytes generates a form of DNA ligase III with distinct
            biochemical properties that may function in meiotic recombination
  JOURNAL   Mol Cell Biol 17 (2), 989-998 (1997)
   PUBMED   9001252
REFERENCE   7  (bases 1 to 8382)
  AUTHORS   Kubota,Y., Nash,R.A., Klungland,A., Schar,P., Barnes,D.E. and
            Lindahl,T.
  TITLE     Reconstitution of DNA base excision-repair with purified human
            proteins: interaction between DNA polymerase beta and the XRCC1
            protein
  JOURNAL   EMBO J 15 (23), 6662-6670 (1996)
   PUBMED   8978692
REFERENCE   8  (bases 1 to 8382)
  AUTHORS   Caldecott,K.W., Tucker,J.D., Stanker,L.H. and Thompson,L.H.
  TITLE     Characterization of the XRCC1-DNA ligase III complex in vitro and
            its absence from mutant hamster cells
  JOURNAL   Nucleic Acids Res 23 (23), 4836-4843 (1995)
   PUBMED   8532526
REFERENCE   9  (bases 1 to 8382)
  AUTHORS   Chen,J., Tomkinson,A.E., Ramos,W., Mackey,Z.B., Danehower,S.,
            Walter,C.A., Schultz,R.A., Besterman,J.M. and Husain,I.
  TITLE     Mammalian DNA ligase III: molecular cloning, chromosomal
            localization, and expression in spermatocytes undergoing meiotic
            recombination
  JOURNAL   Mol Cell Biol 15 (10), 5412-5422 (1995)
   PUBMED   7565692
REFERENCE   10 (bases 1 to 8382)
  AUTHORS   Wei,Y.F., Robins,P., Carter,K., Caldecott,K., Pappin,D.J., Yu,G.L.,
            Wang,R.P., Shell,B.K., Nash,R.A., Schar,P. et al.
  TITLE     Molecular cloning and expression of human cDNAs encoding a novel
            DNA ligase IV and DNA ligase III, an enzyme active in DNA repair
            and recombination
  JOURNAL   Mol Cell Biol 15 (6), 3206-3216 (1995)
   PUBMED   7760816
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB100562.1, BC068005.1,
            BM726768.1, AW166526.1, AA872458.1 and AC004223.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_013975.3.
            
            Summary: This gene is a member of the DNA ligase family. Each
            member of this family encodes a protein that catalyzes the joining
            of DNA ends but they each have a distinct role in DNA metabolism.
            The protein encoded by this gene is involved in excision repair and
            is located in both the mitochondria and nucleus, with translation
            initiation from the upstream start codon allowing for transport to
            the mitochondria and translation initiation from a downstream start
            codon allowing for transport to the nucleus. Additionally,
            alternate transcriptional splice variants, encoding different
            isoforms, have been characterized. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (alpha) represents the longer
            transcript and encodes the longer isoform (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.177858.1,
                                           SRR1803611.130782.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000378526.9/
                                                  ENSP00000367787.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                DB100562.1         15-70
            57-3194             BC068005.1         46-3183
            3195-3440           BM726768.1         415-660
            3441-3683           AW166526.1         1-243               c
            3684-3708           AA872458.1         1-25                c
            3709-8382           AC004223.2         80583-85256         c
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..8382
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="DNA ligase 3"
                     /db_xref="GeneID:3980"
                     /db_xref="HGNC:HGNC:6600"
                     /db_xref="MIM:600940"
     exon            1..111
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            112..662
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..3145
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /EC_number="6.5.1.1"
                     /note="isoform alpha precursor is encoded by transcript
                     variant alpha; ligase II, DNA, ATP-dependent;
                     polydeoxyribonucleotide synthase [ATP] 3; ligase III, DNA,
                     ATP-dependent"
                     /codon_start=1
                     /product="DNA ligase 3 isoform alpha precursor"
                     /protein_id="NP_039269.2"
                     /db_xref="CCDS:CCDS11284.2"
                     /db_xref="GeneID:3980"
                     /db_xref="HGNC:HGNC:6600"
                     /db_xref="MIM:600940"
                     /translation="MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLL
                     HGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGT
                     AGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDL
                     TELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFV
                     TSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADN
                     PSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIF
                     NCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDE
                     QQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDV
                     VERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIK
                     YDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDN
                     KTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEI
                     PNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLN
                     EGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLAR
                     LQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTAD
                     GISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEE
                     NKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSP
                     VKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDM
                     TSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC"
     exon            663..806
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            807..1004
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1156
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1323
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1401
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1570
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1726
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1858
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1938
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2026
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2104
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2228
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2371
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2446
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2593
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2789
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..2911
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     exon            2912..8382
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3425..3430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="hexamer: AATAAA"
     polyA_site      3444
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="major polyA site"
     regulatory      3685..3690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="hexamer: AATGAA"
     polyA_site      3703
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
     polyA_site      4633
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
     regulatory      7781..7786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="hexamer: TATAAA"
     polyA_site      7805
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
     regulatory      7857..7862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
                     /note="hexamer: AATAAA"
     polyA_site      7884
                     /gene="LIG3"
                     /gene_synonym="LIG2; LIG3alpha; MTDPS20"
ORIGIN      
        1 gctctaggac ccggatttaa agagacaggc gctccaaccg tcgtgggctg cccgcggcct
       61 gtaatgagca agttccgagg cctacggtga gcgccggagc cggagaggca gctatatgtc
      121 tttggctttc aagatcttct ttccacaaac cctccgtgca ctcagccgaa aagaactgtg
      181 cctattccga aaacatcact ggcgtgatgt aagacaattc agccagtggt cagaaacaga
      241 tctgcttcat ggacatcccc tcttcctgag aagaaagcct gttctatcat tccagggaag
      301 ccatctaaga tcacgtgcca cctaccttgt tttcttgcca gggttgcatg tgggactctg
      361 cagtggcccc tgtgagatgg ctgagcaacg gttctgtgtg gactatgcca agcgtggcac
      421 agctggctgc aaaaaatgca aggaaaagat tgtgaagggc gtatgccgaa ttggcaaagt
      481 ggtgcccaat cccttctcag agtctggggg tgatatgaaa gagtggtacc acattaaatg
      541 catgtttgag aaactagagc gggcccgggc caccacaaaa aaaatcgagg acctcacaga
      601 gctggaaggc tgggaagagc tggaagataa tgagaaggaa cagataaccc agcacattgc
      661 agatctgtct tctaaggcag caggtacacc aaagaagaaa gctgttgtcc aggctaagtt
      721 gacaaccact ggccaggtga cttctccagt gaaaggcgcc tcatttgtca ccagtaccaa
      781 tccccggaaa ttttctggct tttcagccaa gcccaacaac tctggggaag ccccctcgag
      841 ccccacccct aagagaagtc tgtcttcaag caaatgtgac cccaggcata aggactgtct
      901 gctacgggag tttcgaaagt tatgcgccat ggtggccgat aatcctagct acaacacgaa
      961 gacccagatc atccaggact tccttcggaa aggctcagca ggagatggtt tccacggtga
     1021 tgtgtaccta acagtgaagc tgctgctgcc aggagtcatt aagactgttt acaacttgaa
     1081 cgataagcag attgtgaagc ttttcagtcg catttttaac tgcaacccag atgatatggc
     1141 acgggaccta gagcagggtg acgtgtcaga gacaatcaga gtcttctttg agcagagcaa
     1201 gtctttcccc ccagctgcca agagcctcct taccatccag gaagtggatg agttccttct
     1261 gcggctgtcc aagctcacca aggaggatga gcagcaacag gccctacagg acattgcctc
     1321 caggtgtaca gccaatgacc ttaaatgcat catcaggttg atcaaacatg atctgaagat
     1381 gaactcaggt gcaaaacatg tgttagacgc ccttgacccc aatgcctatg aagccttcaa
     1441 agcctcgcgc aacctgcagg atgtggtgga gcgggtcctt cacaacgcgc aggaggtgga
     1501 gaaggagccg ggccagagac gagctctgag cgtccaggcc tcgctgatga cacctgtgca
     1561 gcccatgttg gcggaggcct gcaagtccgt tgagtatgca atgaagaaat gtcccaatgg
     1621 catgttctct gagatcaagt acgatggaga gcgagtccag gtgcataaga atggagacca
     1681 cttcagctac ttcagccgca gtctcaagcc cgtccttcct cacaaggtgg cccactttaa
     1741 ggactacatt ccccaggctt ttcctggggg ccacagcatg atcttggatt ctgaagtgct
     1801 tctgattgac aacaagacag gcaaaccact gccctttggg actctgggag tacacaagaa
     1861 agcagccttc caggatgcta atgtctgcct gtttgttttt gattgtatct actttaatga
     1921 tgtcagcttg atggacagac ctctgtgtga gcggcggaag tttcttcatg acaacatggt
     1981 tgaaattcca aaccggatca tgttctcaga aatgaagcga gtcacaaaag ctttggactt
     2041 ggctgacatg ataacccggg tgatccagga gggattggag gggctggtgc tgaaggatgt
     2101 gaagggtaca tatgagcctg ggaagcggca ctggctgaaa gtgaagaaag actatttgaa
     2161 cgagggggcc atggccgaca cagctgacct ggtggtcctt ggagccttct atgggcaagg
     2221 gagcaaaggc ggcatgatgt caatcttcct catgggctgc tacgaccctg gcagccagaa
     2281 gtggtgcaca gtcaccaagt gtgcaggagg ccatgatgat gccacgcttg cccgcctgca
     2341 gaatgaacta gacatggtga agatcagcaa ggaccccagc aaaataccca gctggttgaa
     2401 ggtcaacaag atctactatc ctgacttcat cgtcccagac ccaaagaaag ctgccgtgtg
     2461 ggagatcaca ggggctgaat tctccaaatc ggaggctcat acagctgacg ggatctccat
     2521 ccgattccct cgctgcaccc gaatccgaga tgataaggac tggaaatctg ccactaacct
     2581 tccccaactc aaggaactgt accagttgtc caaggagaag gcagacttca ctgtagtggc
     2641 tggagatgag gggagctcca ctacaggggg tagcagtgaa gagaataagg gtccctcagg
     2701 gtctgctgtg tcccgcaagg cccccagcaa gccctcagcc agtaccaaga aagcagaagg
     2761 gaagctgagt aactccaaca gcaaagatgg caacatgcag actgcaaagc cttccgctat
     2821 gaaggtgggg gagaagctgg ccacaaagtc ttctccagtg aaagtagggg agaagcggaa
     2881 agctgctgat gagacgctgt gccaaacaaa ggtattgctg gacatcttca ctggggtgcg
     2941 gctttacttg ccaccctcca caccagactt cagccgtctc agacgctact ttgtggcatt
     3001 cgacggggac ctggtacagg aatttgatat gacttcagcc acgcacgtgc tgggtagcag
     3061 ggacaagaac cctgcggccc agcaggtctc cccagagtgg atttgggcat gtatccggaa
     3121 acggagactg gtagctccct gctaggtttg ctgtcttccc tctccctcag gccatactct
     3181 cctttaccat actactggac tggactcagg ctggaggcag atagacacag tataggggga
     3241 atgggcttgc ttctcccaaa cccaccagtt ctccactgtc tcttctggac caggaattag
     3301 ttgctgtggg tgccacagct gaagtcagtt tgtcttgctg gtttaaatag atctttcaga
     3361 gctgggtgct gggtttgcca tctttttgtt ttctttgaaa agcagcttag ttaccctttt
     3421 tataaataaa atatcttgca gttatctttg tcctttcccc acctacaccc ccaataattt
     3481 ccctagagat taaggagtaa aggctgggct atggcagctc tgtccacaaa gccttctctc
     3541 ccatccttgc ctgttccttt gtacttccag gctcatttta aagttgtatt taaaggactg
     3601 ccctcggaaa tgcttctgtt tagcggaact tgtattcagc ctgacacgct ttgccaggaa
     3661 caaacctcat gtgaaagaaa acaaaatgaa tttttttact ttcttctctg tgttctccta
     3721 ttacatggtg aggatcccca gtttgaaggg aggggactag ggtcaggtag gaaaatgggg
     3781 cctttatgaa gaaaggggag gttatttggg ccactcctct ggagggatag agggctccag
     3841 gaagatctgc atccccaaaa cctggaaaca agactgttct ttaagaataa aaatccacat
     3901 ggggcttgag gccaagaaca gcccttgttg ccaccaacat ggagactttg taccatatcc
     3961 cattcttagt gctcgagtgt tccaacctga agttgaagaa gccaccctgg ctgcacatgc
     4021 catcagccat gactgtgtat gctctggtgg tggtgtctta cttcctcatg actggaggaa
     4081 taattatatg atattgttga acccccaagt attggctgat gaacgtgggc atcagaggcc
     4141 agtagctttc ttggcctaca aagtaaatgg acagtatatt atggaaggat ttgcatccag
     4201 cttcctgttt acaatgggag gtttaggttt cataatgctg gaccagtcga atgcaccaaa
     4261 tatcccaaaa ctcaatcgat tttttttctt ctattcattg gattcgtctg tgtcctactg
     4321 agttttttca tggctggagt attcatgtga atgaaactgc cgggctatct gatgggttag
     4381 agcgccttta agaagaaatc agtggatact ggatttgctc ctgtcaatga agttctaaag
     4441 gctgtaccaa tcctccaata tgaaatgtgg gaaagaatga agagcagcag taaaagaaat
     4501 acctagcgaa aaaaacagga agcatattga agctcggact agaatttctt cttggtatca
     4561 gagagacaag tttatcacag tatttttttt tttcctgctg acctattgat aataccaaca
     4621 atgttgagtg gcattttctt cttagttttt aatttcttaa agaaaatata ctccatatct
     4681 gcagggaaaa aaataaaaat ccacacctga gaacagatcc atacaacctg cttacaaaga
     4741 acagctcctg tgctacctga ggctgattta tttgaagaac aaaaggaaga gagacaattt
     4801 agtgtagaca agtgctattc aatagaactt tttgcagcag tggaaatatc cataaatctg
     4861 cagtatccaa ccaaactagc cacagacagc tgctgagcaa ttgaaatgaa gctggcacaa
     4921 ctaaggaact gaagttctta ttttaattca acttcaaatg gtcacatgtc actagtgact
     4981 accacactgg acagtgcagg tgtagacgga gttcagatca gactcctgaa acttaatggc
     5041 tctaacctta ggcaagttac gttaacatct aagcctcaat ccctccaagt agaatgggtg
     5101 cacctaaatc atagggttgt cctgttgtcc tgagcaacat tatgtaaagc acttagccca
     5161 gagcctagca catagttatc tagagttggg aacgtcactc ctggtgtgca tgtccagacc
     5221 ctacccggga gaagggccta acctatgact ttagaaccct cagttgcagg tgtgcatgtt
     5281 ccagccaaca ctccactctg agtgcaggct catcaggaca ccatatggtt gtaaactggc
     5341 tgccatgtcc caatccttcc agctcttgag ggtcagttct caccttatga acaaattaac
     5401 aggctctgga cctataagat caaggaccac tgcgccccct ggccacccca ccgccccccc
     5461 ccaactttga tctgattgac tttggcttcc acctactgtt taaaatgtgt atgttggggg
     5521 agtagggaca gcacggggga ggggtttact ttcctgactc tagcccagga catttggtgt
     5581 ttttgaatag gcaccattca gccttgcttc agtattagga ataagatact ttttattcca
     5641 tcctctatgg agacaaaaag ctgctcctcc tttgagtact gacctacata gatttccatt
     5701 tctctcttca gcctcaggcc aaacaaacaa ccggacaggg cagcagaaca aggctgtctc
     5761 ctcaggatcc tgccaaaggc actgtcctct ccaacctgga cccagcccca cttgccctta
     5821 ttggttgctt acacccagag tgcacactca caggaaaggg cctctgctgg ctgcaggtga
     5881 tcatcttgtc ccttctctgc cttagtgtgt gttattgcca tttcaatgtc agtggttttt
     5941 tatgtatttt cttccagtct ttgctgctga gatctgactg tatatactat tttgtatcct
     6001 ttcatgtatt tctttcatgt atttcaatgc agccctcaac atcatcatca taaatctctc
     6061 cagcagaatg ctggaggcct ccctcttctg aacctctaca tgctgctggc tggcatcaag
     6121 cagaggtcat cactgggtgc tgaacaggtc agctccctag ggcaatgtga gcaaagtgtt
     6181 gtggctcatc ctcgatcctt tatgcccttt tggcaagatt cctttctctt ctcagtcaca
     6241 gcagatggac acataatctt gttctcccag gggctgcatt ctcagtagtc ttttccctgc
     6301 tccttgggcg cctggtcagg gaggtttgta caagcctgcg cacacacagc agtctgtcac
     6361 gaggctgggc caaccttttt ttttttttta aacagactct tgctctgtca ttcaggctgg
     6421 agtgcagtgg tgcaatctca gctcactgca acctctgctt cccgggttca caagcaattc
     6481 tcatgcctca gcctcccaag tggctaagac ttaacaaaca ggcacacacc atcactcgca
     6541 gctaagtttt tgtattttta gtagagacca cgtttcgcca tgttgtccag actggtctca
     6601 aactcctggc ctcgagtcat ccacgcacct cagcctccca aagtgctggg attacaggcg
     6661 tgagccatgg cgcccagcct gggccaacct tttctgagcc atagtccagc tgtactacag
     6721 ctgtatccaa aaggtaccat gtatctccca gggtaacctg aagacctaac tgggcatact
     6781 aactgtcctc agatttcagt tctttaaggc ccatctcttc tagatcagtg taaatatctg
     6841 aactcacttg gtgcttcaac ccaattggtc ttgggattca tcctttacct acccagctct
     6901 cattctcttc taggttgttc catggcctct tccatatatt acatttgtgg tcttgagcaa
     6961 gttagttagt tacaacaaca ctgagcatct tgaccaaggt cacttggccc aaccacaggc
     7021 taatcccagg ccatgtgcac atcccaggat tagcttgtgg tttatcagtt gtcaattttt
     7081 tttttttttt aatacagggt ctcactctgt caccctggct ggagtgcggt ggcgatcaga
     7141 gctcactgca gcttcaatct cctggctcaa gtgatcctcc cacctcagcc ttccaagtag
     7201 ttgggactac ccacatgcac caccatgcac ggctaatttt ttggtttttt tgggtttttt
     7261 gtttgtgttt ttttttgttt tgttttttga gacagagtct cgctctttcg cccaggccgg
     7321 actgcagtgg cgcaatcttg gctcactgca agctccgcct cccgggttca caccattctc
     7381 ctgcctcagc ctcccgagta gctgggacta caggcacccg ccaccacgcc tggctaattt
     7441 tttgtatttt tagtagagac ggggtttcac cgtgttagcc agggtggtct cgatctcctg
     7501 acctcgtgat ctgcccgcct cagcctccca aagtgctggg attacaggcg tgagccaccg
     7561 cacccggccc ttgtttgttt ttttaagtag agacaaggtc tcactacatt gcccaggttg
     7621 gtctcaaact cctgagctca agcagtcttc ccaccttggc ctcctaaagt gtggaaatta
     7681 tagcatgaac cactgtcagg ctcttttgtc agtcctatca acctctaaca tcctcgcacc
     7741 agacaggaca ggtgtctgta ttgaaacttt attacaaaat tataaagcag agctctgtaa
     7801 caaaataata cacatttggg tttgctttaa cctccaagta agtctgagaa aatcttaata
     7861 aaagccactt gaagtaacaa ttcacatcca agagatttcc acaaatttat acaatgtata
     7921 ttgagcacta gttcctgtac agcttattct tattagtttg gatccaacta ttccaatgta
     7981 ttatgaacca gtcagctatc tgtcttttga aacaagtctt aactgaaatc tcagagtaat
     8041 cagcaaaagc tacggaataa ttctaagaat tagatgtttc catatcatta aaaccaagga
     8101 tccatgaggg gcagaaggga ggattcaaag atttaaaaaa aatcaaattt tagaccttgg
     8161 ttaaatatta actggaatgg gatcttggaa ctcccaactt taatttggtg taataaaaat
     8221 gatgcaaaaa aaaaaaaaat cagggttgtt tgacaccttt tttcctaaag ggaaaccttc
     8281 accaaaaggg gataaaagat ttaaaggcaa aatgagtaaa caacctcagt tttaattctt
     8341 actgaggtta ctaaccagct atcagtatat tattttcgat tt
//
