LOCUS       NM_000894                524 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens luteinizing hormone subunit beta (LHB), mRNA.
ACCESSION   NM_000894
VERSION     NM_000894.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Khamis,H., Rudnizky,S., Melamed,P. and Kaplan,A.
  TITLE     Single molecule characterization of the binding kinetics of a
            transcription factor and its modulation by DNA sequence and
            methylation
  JOURNAL   Nucleic Acids Res 49 (19), 10975-10987 (2021)
   PUBMED   34606618
  REMARK    GeneRIF: Single molecule characterization of the binding kinetics
            of a transcription factor and its modulation by DNA sequence and
            methylation.
REFERENCE   2  (bases 1 to 524)
  AUTHORS   Pnueli,L., Shalev,D., Refael,T., David,C., Boehm,U. and Melamed,P.
  TITLE     Proliferating primary pituitary cells as a model for studying
            regulation of gonadotrope chromatin and gene expression
  JOURNAL   Mol Cell Endocrinol 533, 111349 (2021)
   PUBMED   34090968
  REMARK    GeneRIF: Proliferating primary pituitary cells as a model for
            studying regulation of gonadotrope chromatin and gene expression.
REFERENCE   3  (bases 1 to 524)
  AUTHORS   Zhou,Y., Shen,L., Dong,B., Liu,C., Lv,W., Chi,J., Che,K., Gao,Y.,
            Wang,Y. and Wang,Y.
  TITLE     Elevated circulating luteinizing hormone levels are associated with
            diabetic macroalbuminuria in Chinese men and postmenopausal women:
            A cross-sectional study
  JOURNAL   J Diabetes 12 (11), 819-833 (2020)
   PUBMED   32475064
  REMARK    GeneRIF: Elevated circulating luteinizing hormone levels are
            associated with diabetic macroalbuminuria in Chinese men and
            postmenopausal women: A cross-sectional study.
REFERENCE   4  (bases 1 to 524)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 524)
  AUTHORS   Keutmann,H.T., Hua,Q.X. and Weiss,M.A.
  TITLE     Structure of a receptor-binding fragment from human luteinizing
            hormone beta-subunit determined by [1H]- and [15N]nuclear magnetic
            resonance spectroscopy
  JOURNAL   Mol Endocrinol 6 (6), 904-913 (1992)
   PUBMED   1495492
REFERENCE   6  (bases 1 to 524)
  AUTHORS   Weiss,J., Axelrod,L., Whitcomb,R.W., Harris,P.E., Crowley,W.F. and
            Jameson,J.L.
  TITLE     Hypogonadism caused by a single amino acid substitution in the beta
            subunit of luteinizing hormone
  JOURNAL   N Engl J Med 326 (3), 179-183 (1992)
   PUBMED   1727547
REFERENCE   7  (bases 1 to 524)
  AUTHORS   Weisshaar,G., Hiyama,J., Renwick,A.G. and Nimtz,M.
  TITLE     NMR investigations of the N-linked oligosaccharides at individual
            glycosylation sites of human lutropin
  JOURNAL   Eur J Biochem 195 (1), 257-268 (1991)
   PUBMED   1991473
REFERENCE   8  (bases 1 to 524)
  AUTHORS   Keutmann,H.T., Charlesworth,M.C., Mason,K.A., Ostrea,T., Johnson,L.
            and Ryan,R.J.
  TITLE     A receptor-binding region in human choriogonadotropin/lutropin beta
            subunit
  JOURNAL   Proc Natl Acad Sci U S A 84 (7), 2038-2042 (1987)
   PUBMED   3470775
REFERENCE   9  (bases 1 to 524)
  AUTHORS   Jameson,J.L., Lindell,C.M. and Habener,J.F.
  TITLE     Evolution of different transcriptional start sites in the human
            luteinizing hormone and chorionic gonadotropin beta-subunit genes
  JOURNAL   DNA 5 (3), 227-234 (1986)
   PUBMED   2424697
REFERENCE   10 (bases 1 to 524)
  AUTHORS   Sairam,M.R. and Li,C.H.
  TITLE     Human pituitary lutropin. Isolation, properties, and the complete
            amino acid sequence of the beta-subunit
  JOURNAL   Biochim Biophys Acta 412 (1), 70-81 (1975)
   PUBMED   1191677
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008687.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 15, 2019 this sequence version replaced NM_000894.2.
            
            Summary: This gene is a member of the glycoprotein hormone beta
            chain family and encodes the beta subunit of luteinizing hormone
            (LH). Glycoprotein hormones are heterodimers consisting of a common
            alpha subunit and an unique beta subunit which confers biological
            specificity. LH is expressed in the pituitary gland and promotes
            spermatogenesis and ovulation by stimulating the testes and ovaries
            to synthesize steroids. The genes for the beta chains of chorionic
            gonadotropin and for luteinizing hormone are contiguous on
            chromosome 19q13.3. Mutations in this gene are associated with
            hypogonadism which is characterized by infertility and
            pseudohermaphroditism. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CD107666.1, SRR18074968.1076963.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000649238.3/ ENSP00000497294.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AC008687.5         28375-28399         c
            26-193              AC008687.5         27855-28022         c
            194-524             AC008687.5         27288-27618         c
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..524
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /note="luteinizing hormone subunit beta"
                     /db_xref="GeneID:3972"
                     /db_xref="HGNC:HGNC:6584"
                     /db_xref="MIM:152780"
     exon            1..25
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..436
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /note="interstitial cell stimulating hormone, beta chain;
                     lutropin subunit beta; luteinizing hormone beta subunit;
                     lutropin beta chain; luteinizing hormone beta polypeptide"
                     /codon_start=1
                     /product="lutropin subunit beta precursor"
                     /protein_id="NP_000885.1"
                     /db_xref="CCDS:CCDS12748.1"
                     /db_xref="GeneID:3972"
                     /db_xref="HGNC:HGNC:6584"
                     /db_xref="MIM:152780"
                     /translation="MEMLQGLLLLLLLSMGGAWASREPLRPWCHPINAILAVEKEGCP
                     VCITVNTTICAGYCPTMMRVLQAVLPPLPQVVCTYRDVRFESIRLPGCPRGVDPVVSF
                     PVALSCRCGPCRRSTSDCGGPKDHPLTCDHPQLSGLLFL"
     exon            26..193
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            194..524
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      503..508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /note="hexamer: AATAAA"
     polyA_site      524
                     /gene="LHB"
                     /gene_synonym="CGB4; HH23; LSH-B; LSH-beta"
                     /note="major polyA site"
ORIGIN      
        1 tgcaccaagg atggagatgc tccaggggct gctgctgttg ctgctgctga gcatgggcgg
       61 ggcatgggca tccagggagc cgcttcggcc atggtgccac cccatcaatg ccatcctggc
      121 tgtcgagaag gagggctgcc cagtgtgcat caccgtcaac accaccatct gtgccggcta
      181 ctgccccacc atgatgcgcg tgctgcaggc ggtcctgccg cccctgcctc aggtggtgtg
      241 cacctaccgt gatgtgcgct tcgagtccat ccggctccct ggctgcccgc gtggtgtgga
      301 ccccgtggtc tccttccctg tggctctcag ctgtcgctgt ggaccctgcc gccgcagcac
      361 ctctgactgt gggggtccca aagaccaccc cttgacctgt gaccaccccc aactctcagg
      421 cctcctcttc ctctaaagac cctccccgca gccttccaag tccatcccga ctcctggagc
      481 cctgacaccc cgatcctccc acaataaagg cttctcaatc cgca
//
