LOCUS       NM_016571               5640 bp    mRNA    linear   PRI 03-AUG-2024
DEFINITION  Homo sapiens lengsin, lens protein with glutamine synthetase domain
            (LGSN), transcript variant 1, mRNA.
ACCESSION   NM_016571
VERSION     NM_016571.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Aragam,N., Wang,K.S. and Pan,Y.
  TITLE     Genome-wide association analysis of gender differences in major
            depressive disorder in the Netherlands NESDA and NTR
            population-based samples
  JOURNAL   J Affect Disord 133 (3), 516-521 (2011)
   PUBMED   21621269
  REMARK    GeneRIF: In major depressive disorder genome-wide association
            study, best male-specific SNP was rs9362774 within LGSN gene
REFERENCE   3  (bases 1 to 5640)
  AUTHORS   Nakatsugawa,M., Hirohashi,Y., Torigoe,T., Asanuma,H., Takahashi,A.,
            Inoda,S., Kiriyama,K., Nakazawa,E., Harada,K., Takasu,H.,
            Tamura,Y., Kamiguchi,K., Shijubo,N., Honda,R., Nomura,N.,
            Hasegawa,T., Takahashi,H. and Sato,N.
  TITLE     Novel spliced form of a lens protein as a novel lung cancer
            antigen, Lengsin splicing variant 4
  JOURNAL   Cancer Sci 100 (8), 1485-1493 (2009)
   PUBMED   19459848
  REMARK    GeneRIF: Novel spliced form of a lens protein as a novel lung
            cancer antigen, Lengsin splicing variant 4, is reported.
REFERENCE   4  (bases 1 to 5640)
  AUTHORS   Wyatt,K., White,H.E., Wang,L., Bateman,O.A., Slingsby,C.,
            Orlova,E.V. and Wistow,G.
  TITLE     Lengsin is a survivor of an ancient family of class I glutamine
            synthetases re-engineered by evolution for a role in the vertebrate
            lens
  JOURNAL   Structure 14 (12), 1823-1834 (2006)
   PUBMED   17161372
  REMARK    GeneRIF: Lengsin belongs to the predominantly prokaryotic class 1
            branch of glutamine synthetases but has lost enzyme activity
REFERENCE   5  (bases 1 to 5640)
  AUTHORS   Grassi,F., Moretto,N., Rivetti,C., Cellai,S., Betti,M.,
            Marquez,A.J., Maraini,G. and Ottonello,S.
  TITLE     Structural and functional properties of lengsin, a pseudo-glutamine
            synthetase in the transparent human lens
  JOURNAL   Biochem Biophys Res Commun 350 (2), 424-429 (2006)
   PUBMED   17010935
  REMARK    GeneRIF: A potential chaperone-like role of Lengsin was
            investigated.
REFERENCE   6  (bases 1 to 5640)
  AUTHORS   Wistow,G.
  TITLE     The NEIBank project for ocular genomics: data-mining gene
            expression in human and rodent eye tissues
  JOURNAL   Prog Retin Eye Res 25 (1), 43-77 (2006)
   PUBMED   16005676
  REMARK    Review article
REFERENCE   7  (bases 1 to 5640)
  AUTHORS   Barragan,I., Marcos,I., Borrego,S. and Antinolo,G.
  TITLE     Mutation screening of three candidate genes, ELOVL5, SMAP1 and
            GLULD1 in autosomal recessive retinitis pigmentosa
  JOURNAL   Int J Mol Med 16 (6), 1163-1167 (2005)
   PUBMED   16273301
REFERENCE   8  (bases 1 to 5640)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Behal,A., Touchman,J.W.,
            Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human lens for the NEIBank
            Project: over 2000 non-redundant transcripts, novel genes and
            splice variants
  JOURNAL   Mol Vis 8, 171-184 (2002)
   PUBMED   12107413
  REMARK    GeneRIF: Lengsin is major novel protein of the vertebrate eye lens.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 5640)
  AUTHORS   Wistow,G., Bernstein,S.L., Ray,S., Wyatt,M.K., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human iris for the NEIBank
            Project: steroid-response factors and similarities with retinal
            pigment epithelium
  JOURNAL   Mol Vis 8, 185-195 (2002)
   PUBMED   12107412
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121949.13, AF242388.1,
            BC130368.1, AL590456.5 and AL450346.4.
            
            On May 17, 2019 this sequence version replaced NM_016571.2.
            
            Summary: This gene encodes a protein with similarity to the GS I
            members of the glutamine synthetase superfamily. The encoded
            protein is referred to as a pseudo-glutamine synthetase because it
            has no glutamine synthesis activity and may function as a chaperone
            protein. This protein is localized to the lens and may be
            associated with cataract disease. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF242388.1, BC130368.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145122, SAMN03267766
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370657.9/ ENSP00000359691.4
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL121949.13        4256-4261           c
            7-1454              AF242388.1         1-1448
            1455-1870           BC130368.1         1448-1863
            1871-5606           AL590456.5         101-3836            c
            5607-5640           AL450346.4         168434-168467       c
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q12"
     gene            1..5640
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="lengsin, lens protein with glutamine synthetase
                     domain"
                     /db_xref="GeneID:51557"
                     /db_xref="HGNC:HGNC:21016"
                     /db_xref="MIM:611470"
     exon            1..70
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1570
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="isoform a is encoded by transcript variant 1;
                     glutamate-ammonia ligase (glutamine synthase) domain
                     containing 1; glutamate-ammonia ligase domain-containing
                     protein 1"
                     /codon_start=1
                     /product="lengsin isoform a"
                     /protein_id="NP_057655.2"
                     /db_xref="CCDS:CCDS4964.1"
                     /db_xref="GeneID:51557"
                     /db_xref="HGNC:HGNC:21016"
                     /db_xref="MIM:611470"
                     /translation="MNNEEDLLQEDSTRDEGNETEANSMNTLRRTRKKVTKPYVCSTE
                     VGETDMSNSNDCMRDSSQILTPPQLSSRMKHIRQAMAKNRLQFVRFEATDLHGVSRSK
                     TIPAHFFQEKVSHGVCMPRGYLEVIPNPKDNEMNNIRATCFNSDIVLMPELSTFRVLP
                     WADRTARVICDTFTVTGEPLLTSPRYIAKRQLSHLQASGFSLLSAFIYDFCIFGVPEI
                     LNSKIISFPALTFLNNHDQPFMQELVDGLYHTGANVESFSSSTRPGQMEISFLPEFGI
                     SSADNAFTLRTGVKEVARKYNYIASFFIETGFCDSGILSHSLWDVDRKKNMFCSTSGT
                     EQLTITGKKWLAGLLKHSAALSCLMAPSVSCRKRYSKDRKDLKKSVPTTWGYNDNSCI
                     FNIKCHGEKGTRIENKLGSATANPYLVLAATVAAGLDGLHSSNEVLAGPDESTDFYQV
                     EPSEIPLKLEDALVALEEDQCLRQALGETFIRYFVAMKKYELENEEIAAERNKFLEYF
                     I"
     exon            71..203
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /inference="alignment:Splign:2.1.0"
     exon            204..370
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /inference="alignment:Splign:2.1.0"
     exon            371..5640
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2086..2091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="hexamer: AATAAA"
     polyA_site      2108
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="major polyA site"
     regulatory      4361..4366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="hexamer: AGTAAA"
     polyA_site      4388
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="major polyA site"
     regulatory      5325..5330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="hexamer: AATAAA"
     polyA_site      5343
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
     regulatory      5617..5622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
                     /note="hexamer: AATAAA"
     polyA_site      5640
                     /gene="LGSN"
                     /gene_synonym="GLULD1; LGS"
ORIGIN      
        1 gatattgtgg tttctaacgc tgaacttaaa aagtgttgag atgaataatg aagaggacct
       61 tctgcaggag gactcaacaa gagatgaagg caatgagact gaagccaaca gcatgaacac
      121 attaagaagg acaaggaaga aagtcactaa accatatgtt tgttcaactg aagtgggaga
      181 aacggatatg tccaattcaa atgattgcat gagggacagc agtcaaattt tgaccccacc
      241 tcaactctct tctagaatga aacacattag acaagccatg gccaaaaatc gcctccagtt
      301 tgtacgattt gaagcaacag acctccacgg cgtgtccagg tctaagacta tccctgcaca
      361 cttttttcaa gagaaagtga gccatggtgt ttgcatgccc cgaggttatc ttgaagtgat
      421 accaaatcca aaggacaatg aaatgaataa cataagagcc acatgtttta atagcgacat
      481 agtcctaatg ccagagttat caacctttag agttttgcca tgggctgaca gaactgcaag
      541 agtgatatgt gataccttca ctgtgactgg tgagcctctt ttgacttccc caaggtacat
      601 tgcaaagagg cagctgagcc atctgcaggc ctctggcttt tccctgcttt ctgctttcat
      661 ctatgatttt tgcatttttg gtgtgcccga aattttaaat tcaaagatta tatcttttcc
      721 tgctttaaca tttttaaata accatgatca gcccttcatg caggaacttg ttgatggctt
      781 gtatcacact ggagccaatg tcgagagttt ttcctcctct accaggcctg gtcagatgga
      841 aatctctttc ctgcctgaat ttggcattag ctcagctgat aatgcattta ccctcagaac
      901 aggtgtcaaa gaagtggcaa ggaaatataa ttacattgcc agcttcttca ttgagactgg
      961 attttgtgat tcagggattt tgtctcatag tctctgggat gtcgatagga agaaaaacat
     1021 gttctgcagc acttctggaa ctgagcagct cacgatcact gggaaaaaat ggttggcagg
     1081 actcttgaag cactctgctg cgctcagctg cctgatggcg ccttctgtta gctgccgaaa
     1141 gcgttattcc aaggacagga aagacctgaa gaagagtgtg cctacaacat ggggatacaa
     1201 tgacaacagc tgtatattta atatcaaatg tcatggagag aaaggcaccc ggatagaaaa
     1261 taaactaggc tcagcaacag caaaccctta cttggtgctg gctgcaactg ttgctgccgg
     1321 cttagatgga cttcatagca gtaatgaggt cttggctggt ccagatgaga gcacagactt
     1381 ttaccaagtg gaaccttctg agatcccttt aaaactagaa gatgcccttg tggcactgga
     1441 agaagatcaa tgcctgagac aggctctagg agaaaccttt attcgatatt ttgttgccat
     1501 gaagaaatat gagttggaga atgaagaaat agctgcagag agaaataaat tcttagagta
     1561 ttttatttag aatagagctc acaactactc ctttagacat gtaattgtta cttaaagcta
     1621 atctaccaaa acaaaaagac tgaacttttg taattaacaa cagcaacaat aacaagatta
     1681 cgaatgcttt tgactttttt ttttgtccat ggaatatttg acagatgaag taggactgct
     1741 gagaagattc tgataacaga aacaaacaac aaagttgtgg tgaagctata atatgcaaac
     1801 ttaccagatc ttgtcagtca tttcctatgt gtatgttgac ctggataaga atatccaatt
     1861 ttgggtggca ataaatatat tcgcacatta agaaaaaaaa gacttaagca ttagaaagca
     1921 aaatttaaat gactaaaaaa gaaaaaaaag aaaaggagaa aaaattttaa tggtacacat
     1981 aggttatgat ttccagggag acaaatatac taatatagtc tcgctaatca aataatattg
     2041 tgaatccagg ttgtatgcac cattatggtc tttctaaaca agttaaataa acttttgcca
     2101 aaatataatg acaggtaggc agttttcccc taagtctctt ctcttttcac ccttctccac
     2161 tagctcctga aaagcttatg aacttcaagg ttataatgtc ctcaagaaac aaatccctaa
     2221 ggaatagaat tggagctgag aacaacgagg tcagatttta aaagtagtta aagcctttct
     2281 tttaacaatg cctcatatag aagttcaata cacaaagtat agatagaaag atgtttgtgt
     2341 gtgagggtag gtgggctctg tctactctgc ttcattcttt gaaactagat gttcctctga
     2401 aacctctagt gctctgtgga gtgtgagaac aactgcctgg ctagccaggg ctatgtttgg
     2461 agtttaagtt tgttattcaa attactgaat ctgctgctca cagaggttgg tacctcccca
     2521 gagaatatta cctgttccag gcctttgttt gaatgagata atattgtaac tatcacaaaa
     2581 gtttagctgc ttttgggaag aagtccaaaa tccacttact agcaaatgaa acattattct
     2641 actgcttagg atcaaacagc agattttatg ttcaattaaa actgactgta gtctcaattt
     2701 agatagcaat tgcacaagca gaggattaga atttggctca gatcctctta ttaaaattca
     2761 tattactcag tagcttaaat agtgaaagtc agtctatcct tcactaatta ctcccttctt
     2821 ttttcctttg agcaggagaa gtgtgtttgg atacataaga agaaagttcc cagcactttg
     2881 ggaggccaag gcaggaggat tgcttgaggc caggagtttg agtccagcct ggaccacata
     2941 gtgagacccc tgtttctaca aaaaaaaaaa aaaaaatgcc tcgcatggtg gcacatgcct
     3001 gtagtcccag ctacgtggga ggctgagaag ggaggatcgc ttgagcctgg gagtttcagg
     3061 ctgcagtgag ccataatcac accactgcac tccagcttgg gtgacagagt gagacactgt
     3121 cttaaaaaaa agagaagaat aaaattcatg gatgcatatg gggtagagag aaagatagtg
     3181 tagggtagct gggggaagga agggaacctg tcctgaaacc tccagaatga aatcccacac
     3241 attacatcat gggagggata aggaagactc tcatgccaat tttctagctg tctgctagaa
     3301 aattcccctc tgttcatttt cccctctgtt ctcagtaata aaacctcaat atgttctttg
     3361 aaagcaatga aaaatgaaat gatatttctg agccttcatt aaggttggat gtggtcatgt
     3421 tattaggttc agtgcaaaga ggtgaaagta gaagtgttgc atataagttc tttaggacat
     3481 tttcttttct tttcttgtat tttttttttt tttccgagat ggagtctcgc tttgtcaccc
     3541 aggctgaagt gcaatgtggg ggggtcttgg ctcactgcaa cctctgcccc ctgggttcaa
     3601 gtaattctcc tgcctcagcc tcccaagtag ctgggatccc aggtgcctgc taccatgcct
     3661 ggccttttag tagagatggg gtttcaccat attggccagt ctggtcttga actcctgaac
     3721 tcaggtgatc agcctgcctt ggcctcccaa agtgctagga ttacaggcat gagccaccat
     3781 gccctaccac actttagaac atttttttaa atgacggatg ctgtgtgctc tgccctttct
     3841 ttctcttgct gccttcaccc ttcctgctga gtggcctaca gataatgtgg ctaggattat
     3901 gtccttggga agggaggttt gctccacagg gtacagtaat aaggtggaat ctctgctttg
     3961 tagagcagtt tgacttgcct acatccagat gtttacctga gagagaacca aacttctatc
     4021 ttctttaagc cacaatgatt ttgattcttt gttagttgca atgaaattta ttttctcttt
     4081 tccatgtgtg tgttgtatgt gtgtttgtgt gtgagtgtgt gcatatgagt ggggaatttg
     4141 agggatttgg ggatgaaaga acaaaggtat aagaaccagt gaaagacgac ttcctcaata
     4201 ttcccacttc tagagaggat aatctgagtt aatcaaaggg atccacacat ccaaaaaatg
     4261 ggattggctc taacccatcc ctataaagtg gcatatccct ttatagtcca cgggccttaa
     4321 tgaaacatgt ctaatgcatt gacttcattt taccataatg agtaaagatg ctgtaaaaag
     4381 tctgataatc acagtgtttg aagccgtttt ttcccctttc gcattattag cataacttaa
     4441 aaacacagta ctcaggatgc catgtgaagg aattatataa ctaggaatat tatcttcagt
     4501 attaagtgta cagtcgtgag tcaaagaaaa tattcagcca tagattacaa atcagttgat
     4561 aacgtaagtt ggtgtggcag tttccaccaa gatttcagtt aggtgtgtgc tgatgtatat
     4621 tcttgttctt taaggcaacc caaaaagagg cattggccag cagagttcag ggccaaagac
     4681 aaattggggt aggtacatgt aaaaatcagt tatctatttt tctatttctt tcatctgttc
     4741 ataggctttc tattgttgaa aggactttct gctgatgctt ctaccttctt ggtctgggtt
     4801 aagagggtca gacattcatt cagcagcact tattgagcac catttgtgtg ctaagccttc
     4861 agctgggagc catcatacta ggggagacca aaacaaagat gagcagacaa tgaaagagtt
     4921 ttggatctgt gtatcttaag cagaacataa ataatttttt ttgtagacct aatgctgaaa
     4981 aaagctattt ggaaagcttc tttatggctc gaaaagggtg ccttataaat agctttacaa
     5041 aaattgcttt cttattttca tttactataa ttgttctaat caaagcttag gggaaaaatg
     5101 tgattactga atttaaagtt ggtaattaaa tgagtataca taagaaatgt aaattaaaag
     5161 cgataatatt ttaagactag aaattaaaac attagtattg tagtaacaag tttctatttt
     5221 caaggacatt ctataatcta aaatttcatt ttacagtgtg tgaataacaa caacgcttga
     5281 aatgattatt tgaaggcaat gaaagatcaa aaaactttaa tctgaataaa aattgaaagc
     5341 atacagtgaa gtctgagctt atcagtaaaa cagaacagtc tattgacata tctccagtca
     5401 cctgtttgac aaaatacact caccaatatg tttatgatat aggtatgact ggacaatacc
     5461 tttaaccttg ggttttctat gacaggggta agaatttgtg tctttaaaca ttacctatgt
     5521 gcacagtgcc ttccctgtta aagctcttct catgctgcct ataattaggg gtttacatga
     5581 tacttcctaa ttgctaatta ggatgtgaat tctcagaata aaaggtacag atcttactta
//
