LOCUS       NM_005606               1980 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens legumain (LGMN), transcript variant 1, mRNA.
ACCESSION   NM_005606
VERSION     NM_005606.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Jiang,Y., Li,L., Wu,R., Wu,L., Zhang,B., Wang,J.Z., Liu,R., Liu,F.,
            Wang,J. and Wang,X.
  TITLE     c-Src regulates delta-secretase activation and truncated Tau
            production by phosphorylating the E3 ligase Traf6
  JOURNAL   J Biol Chem 299 (12), 105462 (2023)
   PUBMED   37977223
  REMARK    GeneRIF: c-Src regulates delta-secretase activation and truncated
            Tau production by phosphorylating the E3 ligase Traf6.
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Zhang,W., Cao,L., Yang,J., Zhang,S., Zhao,J., Shi,Z., Liao,K.,
            Wang,H., Chen,B., Qian,Z., Xu,H., Wu,L., Liu,H., Wang,H., Ma,C.,
            Qiu,Y., Ge,J., Chen,J. and Lin,Y.
  TITLE     AEP-cleaved DDX3X induces alternative RNA splicing events to
            mediate cancer cell adaptation in harsh microenvironments
  JOURNAL   J Clin Invest 134 (3), e173299 (2023)
   PUBMED   37988165
  REMARK    GeneRIF: AEP-cleaved DDX3X induces alternative RNA splicing events
            to mediate cancer cell adaptation in harsh microenvironments.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1980)
  AUTHORS   Tan,Y.W., Teo,F.M.S., Ler,S.G., Alli-Shaik,A., Nyo,M., Chong,C.Y.,
            Tan,N.W.H., Wang,R.Y.L., Gunaratne,J. and Chu,J.J.H.
  TITLE     Potential relevance of salivary legumain for the clinical
            diagnostic of hand, foot, and mouth disease
  JOURNAL   J Med Virol 95 (11), e29243 (2023)
   PUBMED   38009231
  REMARK    GeneRIF: Potential relevance of salivary legumain for the clinical
            diagnostic of hand, foot, and mouth disease.
REFERENCE   4  (bases 1 to 1980)
  AUTHORS   Deng,Y., Fan,Y., Wu,D., Zhang,Z., Zhang,M., Huang,Z. and Gao,Y.
  TITLE     Relationship between Increased Plasma Levels of Legumain and
            Properties of Coronary Atherosclerotic Plaque
  JOURNAL   Arq Bras Cardiol 120 (11), e20230395 (2023)
   PUBMED   37909538
  REMARK    GeneRIF: Relationship between Increased Plasma Levels of Legumain
            and Properties of Coronary Atherosclerotic Plaque.
REFERENCE   5  (bases 1 to 1980)
  AUTHORS   Rodriguez-Silvestre,P., Laub,M., Krawczyk,P.A., Davies,A.K.,
            Schessner,J.P., Parveen,R., Tuck,B.J., McEwan,W.A., Borner,G.H.H.
            and Kozik,P.
  TITLE     Perforin-2 is a pore-forming effector of endocytic escape in
            cross-presenting dendritic cells
  JOURNAL   Science 380 (6651), 1258-1265 (2023)
   PUBMED   37347855
REFERENCE   6  (bases 1 to 1980)
  AUTHORS   Halfon,S., Patel,S., Vega,F., Zurawski,S. and Zurawski,G.
  TITLE     Autocatalytic activation of human legumain at aspartic acid
            residues
  JOURNAL   FEBS Lett 438 (1-2), 114-118 (1998)
   PUBMED   9821970
REFERENCE   7  (bases 1 to 1980)
  AUTHORS   Chen,J.M., Dando,P.M., Stevens,R.A., Fortunato,M. and Barrett,A.J.
  TITLE     Cloning and expression of mouse legumain, a lysosomal endopeptidase
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 111-117 (1998)
   PUBMED   9742219
REFERENCE   8  (bases 1 to 1980)
  AUTHORS   Chen,J.M., Dando,P.M., Rawlings,N.D., Brown,M.A., Young,N.E.,
            Stevens,R.A., Hewitt,E., Watts,C. and Barrett,A.J.
  TITLE     Cloning, isolation, and characterization of mammalian legumain, an
            asparaginyl endopeptidase
  JOURNAL   J Biol Chem 272 (12), 8090-8098 (1997)
   PUBMED   9065484
REFERENCE   9  (bases 1 to 1980)
  AUTHORS   Tanaka,T., Inazawa,J. and Nakamura,Y.
  TITLE     Molecular cloning of a human cDNA encoding putative cysteine
            protease (PRSC1) and its chromosome assignment to 14q32.1
  JOURNAL   Cytogenet Cell Genet 74 (1-2), 120-123 (1996)
   PUBMED   8893817
REFERENCE   10 (bases 1 to 1980)
  AUTHORS   Friedman,J. and Razin,A.
  TITLE     Studies on the biological role of DNA methylation. II. Role of
            phiX174 DNA methylation in the process of viral progeny DNA
            synthesis
  JOURNAL   Nucleic Acids Res 3 (10), 2665-2675 (1976)
   PUBMED   136644
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL132987.4, AL136332.5,
            BC013678.1 and CD366588.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005606.6.
            
            Summary: This gene encodes a cysteine protease that has a strict
            specificity for hydrolysis of asparaginyl bonds. This enzyme may be
            involved in the processing of bacterial peptides and endogenous
            proteins for MHC class II presentation in the lysosomal/endosomal
            systems. Enzyme activation is triggered by acidic pH and appears to
            be autocatalytic. Protein expression occurs after monocytes
            differentiate into dendritic cells. A fully mature, active enzyme
            is produced following lipopolysaccharide expression in mature
            dendritic cells. Overexpression of this gene may be associated with
            the majority of solid tumor types. This gene has a pseudogene on
            chromosome 13. Several alternatively spliced transcript variants
            have been described, but the biological validity of only two has
            been determined. These two variants encode the same isoform.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291560.1, AK291534.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334869.9/ ENSP00000334052.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               AL132987.4         136812-136950
            140-306             AL132987.4         152624-152790
            307-404             AL132987.4         166595-166692
            405-486             AL136332.5         34918-34999
            487-572             AL136332.5         36158-36243
            573-648             AL136332.5         37928-38003
            649-711             AL136332.5         38494-38556
            712-778             AL136332.5         39508-39574
            779-897             AL136332.5         40424-40542
            898-987             AL136332.5         40631-40720
            988-1188            AL136332.5         42507-42707
            1189-1359           AL136332.5         45726-45896
            1360-1427           AL136332.5         47672-47739
            1428-1915           AL136332.5         48018-48505
            1916-1973           BC013678.1         948-1005
            1974-1980           CD366588.1         20-26               c
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..1980
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /note="legumain"
                     /db_xref="GeneID:5641"
                     /db_xref="HGNC:HGNC:9472"
                     /db_xref="MIM:602620"
     exon            1..139
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            140..306
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..1470
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /EC_number="3.4.22.34"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; protease, cysteine, 1 (legumain); cysteine
                     protease 1; asparaginyl endopeptidase; protease, cysteine
                     1"
                     /codon_start=1
                     /product="legumain isoform 1 preproprotein"
                     /protein_id="NP_005597.3"
                     /db_xref="CCDS:CCDS9904.1"
                     /db_xref="GeneID:5641"
                     /db_xref="HGNC:HGNC:9472"
                     /db_xref="MIM:602620"
                     /translation="MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYR
                     HQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKD
                     YTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNED
                     LHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYA
                     CYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTIS
                     TMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEE
                     IQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFN
                     WHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY"
     exon            307..404
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            405..486
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            487..572
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..648
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            649..711
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            712..778
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            779..897
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            898..987
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1188
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1359
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1427
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1980
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1955..1960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /note="hexamer: AATAAA"
     polyA_site      1980
                     /gene="LGMN"
                     /gene_synonym="AEP; LGMN1; PRSC1"
                     /note="major polyA site"
ORIGIN      
        1 aatcacagca gtgccgacgt cgtgggtgtt tggtgtgagg ctgcgagccg ccgcgagttc
       61 tcacggtccc gccggcgcca ccaccgcggt cactcaccgc cgccgccgcc accactgcca
      121 ccacggtcgc ctgccacagg tgtctgcaat tgaactccaa ggtgcagaat ggtttggaaa
      181 gtagctgtat tcctcagtgt ggccctgggc attggtgccg ttcctataga tgatcctgaa
      241 gatggaggca agcactgggt ggtgatcgtg gcaggttcaa atggctggta taattatagg
      301 caccaggcag acgcgtgcca tgcctaccag atcattcacc gcaatgggat tcctgacgaa
      361 cagatcgttg tgatgatgta cgatgacatt gcttactctg aagacaatcc cactccagga
      421 attgtgatca acaggcccaa tggcacagat gtctatcagg gagtcccgaa ggactacact
      481 ggagaggatg ttaccccaca aaatttcctt gctgtgttga gaggcgatgc agaagcagtg
      541 aagggcatag gatccggcaa agtcctgaag agtggccccc aggatcacgt gttcatttac
      601 ttcactgacc atggatctac tggaatactg gtttttccca atgaagatct tcatgtaaag
      661 gacctgaatg agaccatcca ttacatgtac aaacacaaaa tgtaccgaaa gatggtgttc
      721 tacattgaag cctgtgagtc tgggtccatg atgaaccacc tgccggataa catcaatgtt
      781 tatgcaacta ctgctgccaa ccccagagag tcgtcctacg cctgttacta tgatgagaag
      841 aggtccacgt acctggggga ctggtacagc gtcaactgga tggaagattc ggacgtggaa
      901 gatctgacta aagagaccct gcacaagcag taccacctgg taaaatcgca caccaacacc
      961 agccacgtca tgcagtatgg aaacaaaaca atctccacca tgaaagtgat gcagtttcag
     1021 ggtatgaaac gcaaagccag ttctcccgtc cccctacctc cagtcacaca ccttgacctc
     1081 acccccagcc ctgatgtgcc tctcaccatc atgaaaagga aactgatgaa caccaatgat
     1141 ctggaggagt ccaggcagct cacggaggag atccagcggc atctggatgc caggcacctc
     1201 attgagaagt cagtgcgtaa gatcgtctcc ttgctggcag cgtccgaggc tgaggtggag
     1261 cagctcctgt ccgagagagc cccgctcacg gggcacagct gctacccaga ggccctgctg
     1321 cacttccgga cccactgctt caactggcac tcccccacgt acgagtatgc gttgagacat
     1381 ttgtacgtgc tggtcaacct ttgtgagaag ccgtatccgc ttcacaggat aaaattgtcc
     1441 atggaccacg tgtgccttgg tcactactga agagctgcct cctggaagct tttccaagtg
     1501 tgagcgcccc accgactgtg tgctgatcag agactggaga ggtggagtga gaagtctccg
     1561 ctgctcgggc cctcctgggg agcccccgct ccagggctcg ctccaggacc ttcttcacaa
     1621 gatgacttgc tcgctgttac ctgcttcccc agtcttttct gaaaaactac aaattagggt
     1681 gggaaaagct ctgtattgag aagggtcata tttgctttct aggaggtttg ttgttttgcc
     1741 tgttagtttt gaggagcagg aagctcatgg gggcttctgt agcccctctc aaaaggagtc
     1801 tttattctga gaatttgaag ctgaaacctc tttaaatctt cagaatgatt ttattgaaga
     1861 gggccgcaag ccccaaatgg aaaactgttt ttagaaaata tgatgatttt tgattgcttt
     1921 tgtatttaat tctgcaggtg ttcaagtctt aaaaaataaa gatttataac agaacccaaa
//
