LOCUS       NM_012318               5371 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens leucine zipper and EF-hand containing transmembrane
            protein 1 (LETM1), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_012318
VERSION     NM_012318.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,L., Liu,W. and Li,M.
  TITLE     Circ_0061140 Contributes to Ovarian Cancer Progression by Targeting
            miR-761/LETM1 Signaling
  JOURNAL   Biochem Genet 61 (2), 628-650 (2023)
   PUBMED   36056285
  REMARK    GeneRIF: Circ_0061140 Contributes to Ovarian Cancer Progression by
            Targeting miR-761/LETM1 Signaling.
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Austin,S., Mekis,R., Mohammed,S.E.M., Scalise,M., Wang,W.A.,
            Galluccio,M., Pfeiffer,C., Borovec,T., Parapatics,K., Vitko,D.,
            Dinhopl,N., Demaurex,N., Bennett,K.L., Indiveri,C. and
            Nowikovsky,K.
  TITLE     TMBIM5 is the Ca2+ /H+ antiporter of mammalian mitochondria
  JOURNAL   EMBO Rep 23 (12), e54978 (2022)
   PUBMED   36321428
REFERENCE   3  (bases 1 to 5371)
  AUTHORS   Kaiyrzhanov,R., Mohammed,S.E.M., Maroofian,R., Husain,R.A.,
            Catania,A., Torraco,A., Alahmad,A., Dutra-Clarke,M., Gronborg,S.,
            Sudarsanam,A., Vogt,J., Arrigoni,F., Baptista,J., Haider,S.,
            Feichtinger,R.G., Bernardi,P., Zulian,A., Gusic,M., Efthymiou,S.,
            Bai,R., Bibi,F., Horga,A., Martinez-Agosto,J.A., Lam,A., Manole,A.,
            Rodriguez,D.P., Durigon,R., Pyle,A., Albash,B., Dionisi-Vici,C.,
            Murphy,D., Martinelli,D., Bugiardini,E., Allis,K., Lamperti,C.,
            Reipert,S., Risom,L., Laugwitz,L., Di Nottia,M., McFarland,R.,
            Vilarinho,L., Hanna,M., Prokisch,H., Mayr,J.A., Bertini,E.S.,
            Ghezzi,D., Ostergaard,E., Wortmann,S.B., Carrozzo,R., Haack,T.B.,
            Taylor,R.W., Spinazzola,A., Nowikovsky,K. and Houlden,H.
  TITLE     Bi-allelic LETM1 variants perturb mitochondrial ion homeostasis
            leading to a clinical spectrum with predominant nervous system
            involvement
  JOURNAL   Am J Hum Genet 109 (9), 1692-1712 (2022)
   PUBMED   36055214
REFERENCE   4  (bases 1 to 5371)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 5371)
  AUTHORS   Zhao,Q., Chen,S. and Chen,L.
  TITLE     LETM1 (leucine zipper-EF-hand-containing transmembrane protein 1)
            silence reduces the proliferation, invasion, migration and
            angiogenesis in esophageal squamous cell carcinoma via KIF14
            (kinesin family member 14)
  JOURNAL   Bioengineered 12 (1), 7656-7665 (2021)
   PUBMED   34605738
  REMARK    GeneRIF: LETM1 (leucine zipper-EF-hand-containing transmembrane
            protein 1) silence reduces the proliferation, invasion, migration
            and angiogenesis in esophageal squamous cell carcinoma via KIF14
            (kinesin family member 14).
REFERENCE   6  (bases 1 to 5371)
  AUTHORS   Hasegawa,A. and van der Bliek,A.M.
  TITLE     Inverse correlation between expression of the Wolfs Hirschhorn
            candidate gene Letm1 and mitochondrial volume in C. elegans and in
            mammalian cells
  JOURNAL   Hum Mol Genet 16 (17), 2061-2071 (2007)
   PUBMED   17606466
  REMARK    GeneRIF: Here, we present cellular and biochemical analysis of
            Letm1
REFERENCE   7  (bases 1 to 5371)
  AUTHORS   Nowikovsky,K., Froschauer,E.M., Zsurka,G., Samaj,J., Reipert,S.,
            Kolisek,M., Wiesenberger,G. and Schweyen,R.J.
  TITLE     The LETM1/YOL027 gene family encodes a factor of the mitochondrial
            K+ homeostasis with a potential role in the Wolf-Hirschhorn
            syndrome
  JOURNAL   J Biol Chem 279 (29), 30307-30315 (2004)
   PUBMED   15138253
REFERENCE   8  (bases 1 to 5371)
  AUTHORS   Schlickum,S., Moghekar,A., Simpson,J.C., Steglich,C., O'Brien,R.J.,
            Winterpacht,A. and Endele,S.U.
  TITLE     LETM1, a gene deleted in Wolf-Hirschhorn syndrome, encodes an
            evolutionarily conserved mitochondrial protein
  JOURNAL   Genomics 83 (2), 254-261 (2004)
   PUBMED   14706454
  REMARK    GeneRIF: LETM1 is evolutionarily conserved throughout the
            eukaryotic kingdom and located in the mitochondria.
REFERENCE   9  (bases 1 to 5371)
  AUTHORS   Endele,S., Fuhry,M., Pak,S.J., Zabel,B.U. and Winterpacht,A.
  TITLE     LETM1, a novel gene encoding a putative EF-hand Ca(2+)-binding
            protein, flanks the Wolf-Hirschhorn syndrome (WHS) critical region
            and is deleted in most WHS patients
  JOURNAL   Genomics 60 (2), 218-225 (1999)
   PUBMED   10486213
REFERENCE   10 (bases 1 to 5371)
  AUTHORS   Battaglia,A., Carey,J.C. and South,S.T.
  TITLE     Wolf-Hirschhorn Syndrome - RETIRED CHAPTER, FOR HISTORICAL
            REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301362
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF061025.1 and AC105448.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_012318.2.
            
            Summary: This gene encodes a protein that is localized to the inner
            mitochondrial membrane. The protein functions to maintain the
            mitochondrial tubular shapes and is required for normal
            mitochondrial morphology and cellular viability. Mutations in this
            gene cause Wolf-Hirschhorn syndrome, a complex malformation
            syndrome caused by the deletion of parts of the distal short arm of
            chromosome 4. Related pseudogenes have been identified on
            chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because transcript sequence consistent with
            the reference genome assembly was not available for all regions of
            the RefSeq transcript. The extent of this transcript is supported
            by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.244606.1,
                                           SRR1660809.61502.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000302787.3/
                                                  ENSP00000305653.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1996              AF061025.1         92-2087
            1997-1997           AC105448.5         5819-5819           c
            1998-2586           AF061025.1         2089-2677
            2587-5371           AC105448.5         431-3215            c
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.3"
     gene            1..5371
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="leucine zipper and EF-hand containing transmembrane
                     protein 1"
                     /db_xref="GeneID:3954"
                     /db_xref="HGNC:HGNC:6556"
                     /db_xref="MIM:604407"
     exon            1..288
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..2426
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="Mdm38 homolog; LETM1 and EF-hand domain-containing
                     protein 1, mitochondrial; leucine zipper-EF-hand
                     containing transmembrane protein 1; mitochondrial
                     proton/calcium exchanger protein; electroneutral
                     mitochondrial K(+)/H(+)exchanger"
                     /codon_start=1
                     /product="mitochondrial proton/calcium exchanger protein
                     precursor"
                     /protein_id="NP_036450.1"
                     /db_xref="CCDS:CCDS3355.1"
                     /db_xref="GeneID:3954"
                     /db_xref="HGNC:HGNC:6556"
                     /db_xref="MIM:604407"
                     /translation="MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGL
                     RNCLNVPFGCCTPIHPVYTSSRGDHLGCWALRPECLRIVSRAPWTSTSVGFVAVGPQC
                     LPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPAEVVVKKSLGQRVLDE
                     LKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLVPFLVFVVV
                     PFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNK
                     AAKGSATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLE
                     LQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTED
                     RLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQV
                     KVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPG
                     TEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEK
                     EELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLIS
                     QLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGK
                     VNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS"
     exon            289..349
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            350..800
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            801..944
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1082
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1286
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1406
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1538
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1682
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1814
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..1949
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2137
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2138..2276
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..5371
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2536..2541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="hexamer: AATAAA"
     polyA_site      2561
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="major polyA site"
     regulatory      5343..5348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="hexamer: ATTAAA"
     polyA_site      5371
                     /gene="LETM1"
                     /gene_synonym="CONDMIM; KHE; Mdm38; SLC55A1"
                     /note="major polyA site"
ORIGIN      
        1 gccccgcgcc tccggacgtc tgtgccggga caaggccgcc gctggtgccg ggtccttgag
       61 gagagcgcct cccgtccgag gccagccgcc tctgtcagcc gtccgcgcgg gccgggtctg
      121 aagcgccgcc gggacggcga agagccgcgg ccgccgcgga gaaggaggca gcgcaggagg
      181 ccggagcggc cgccgcgccc gagcacatgg cgtccatctt actgaggagc tgccgcggcc
      241 gggcgcccgc ccgcctcccg ccgccgcctc ggtacaccgt cccgcggggt agtccagggg
      301 atcctgctca tctcagctgt gccagcaccc tggggttgag gaactgcctg aatgttccat
      361 ttggctgctg cactcccatc caccctgtgt acacatcctc cagaggcgat cacctcggct
      421 gttgggctct gaggcccgag tgccttcgca tagtgtcgag agcgccatgg acctctacct
      481 ctgtgggttt tgtggctgtg ggacctcagt gccttcctgt gcgtggctgg cactcttcgc
      541 gccctgttcg cgatgactcg gtagtagaga agtccctcaa gtccttgaag gacaagaaca
      601 agaagctgga ggaaggcggc ccggtgtaca gcccccccgc agaggtggtg gtgaagaagt
      661 ccctggggca gcgggtgctg gacgagctga agcactacta ccatggcttc cgcctgctat
      721 ggatcgacac caagatcgcg gcacgcatgc tctggcgcat cctcaacggc cacagcctga
      781 cccgccggga gcgcaggcag tttctccgga tctgcgctga cctcttccgc ctggtgccgt
      841 tccttgtgtt cgtggtggtg ccgttcatgg agtttctgct gcctgttgct gtgaagctct
      901 tccccaacat gttgccatcc acatttgaga ctcagtcact caaggaggag aggctgaaga
      961 aggagcttcg ggtcaagctg gagctggcca agttcctcca ggacaccatc gaggagatgg
     1021 ccttgaagaa caaggcagcc aagggcagcg ccaccaaaga cttctctgtg tttttccaga
     1081 agatccggga aacaggggag aggcccagca atgaggaaat catgcgtttt tccaaattat
     1141 ttgaggatga gctgaccctg gacaacctga cacggccgca gctggtggcc ctgtgcaagc
     1201 tgctggagct acagtccatc ggcaccaaca acttcctgcg cttccagctt accatgcggc
     1261 tgcgctccat aaaggcagac gacaagctga ttgctgagga aggggtggac agcctgaatg
     1321 tcaaggagct gcaggcagcg tgtcgggcac gaggcatgcg ggccctgggc gtcacggaag
     1381 accgcctgag gggtcagctg aagcagtggc tggacctgca cctgcatcag gagatcccca
     1441 catcgctgct catcctgtcc cgggccatgt acctcccgga caccctctct ccagccgacc
     1501 agctcaagtc cacactgcag accctcccag agattgtggc aaaggaagca caggtgaaag
     1561 tggccgaggt ggagggcgag caggtggaca acaaggccaa gctggaggcc acgctgcagg
     1621 aggaggcggc catccagcag gagcaccgtg agaaggagct gcagaagcgc tcggaggtgg
     1681 cgaaggattt tgagcccgaa cgtgtggtag ctgctcccca aaggccgggg accgagccac
     1741 agccagaaat gcctgacaca gtcctgcagt cagagacctt gaaggacact gccccggtgc
     1801 tggagggctt gaaggaggaa gagatcacga aggaggaaat cgacatcctc agcgatgcct
     1861 gctctaagct gcaggagcag aagaagtcac tcaccaagga gaaggaggag ctggagctgc
     1921 tgaaggagga tgtgcaggac tacagcgagg acttgcagga gatcaagaag gaactttcaa
     1981 agactggtga agaaaagtac gtggaagaat ctaaagccag caagagattg acaaaaaggg
     2041 tgcagcaaat gatcgggcag atcgatggct tgatctcgca gctggagatg gaccagcagg
     2101 ctggcaagct ggccccggcc aacggcatgc ccacggggga gaacgtcatc agtgtcgctg
     2161 agctcatcaa cgccatgaag caagtcaagc acattcccga aagcaagctc accagcctgg
     2221 ccgcagcact ggatgaaaac aaggatggca aggtcaacat cgacgacctc gtcaaggtga
     2281 ttgagctggt ggacaaagaa gatgttcaca tctccaccag ccaggtggct gagattgtag
     2341 caacactgga aaaagaggag aaggtggagg agaaggagaa ggccaaagag aaggcagaga
     2401 aggaggtcgc agaggtgaag agctagaacc actggcctgg gcacctgtcc tcctgctgtg
     2461 ccgtcaccct ggcaagggcc gtgagggcga ttgctttgtg gtgattctca gtggctcatc
     2521 taatattttg gctggaataa atcagagact tccataatca agtaaatttt aattttcatc
     2581 attccacgga gattcagtct gtctggaatc ccggaacccc tccacagaat cgtgtctgga
     2641 tccacactgt ggtgtggctg ccacggcctc ctggctccag aggcagctgg gctgggccaa
     2701 ggcaggaagg cgcccccatg tgtgtggcct cagtctcagc atctgactgt gctgccctgt
     2761 ccccagggaa gagaatgagg accacgtgga ctccgcccac acagagctgg ctgctgtgcc
     2821 caccctcagg ggcgtcagga gacacagcct ggcctcctca gggctgaaga tgcctccatt
     2881 cgcctgtgga ccttgatttc tagatttcag tgtcaataga cctgtcttcc tgcacacttt
     2941 cagttggatg tgggtcattg tggagacaga ggtgtctgcc atctctgtgg cctctggaga
     3001 gtgacgtctc cctgctggga gtgctggttc tcacgtgcgg tttcccttgt gtatcggagc
     3061 tctttctcgg ctttctattt cccccagttc tttaagcagt caattggcac agagtttccc
     3121 acgggggctg cagtggattc actgtgtcag ggatgagcct ggcttggggg ttggtggggc
     3181 tctgaagcgc atttggggtt ctttgtagct tctaatgtga ggtttgagtc cagtgcgccc
     3241 acagcagcat gcccttctca ccccttgcag tgttgcaggc tcaggcccct gggctccttc
     3301 agcaagcagg ctagtcaatg aggcatgagg atccggcaca gggcatcccc tggggcttca
     3361 agggcaatac ccccgtgctt agggtttgcc ctgtgccctc tgggtggggt ggcctccccg
     3421 cactcggcag catggccagg ctggccaggg cgtgggcagg tggtgtcctg tggcacctcc
     3481 atcctcctgc ccagccggct gtgtcacact catcttttta aggtcaggtt ggttcctggc
     3541 aaaaatgtac ctccaggggc ctccaagcat aggatttgga agacaggaac ggcacaggcg
     3601 tccaggaaag cagctgcact cagacaatgc cttctccatt acttgaagct tctttctgtt
     3661 cagccattaa agaaacttga caaaatagga acaggagata tttctcaatt gtaaaccttt
     3721 gtgcagggac agttggcttc cagaggcttt cagctttcag ttatttgaga agtttgtttt
     3781 agatccttaa ctaatattta tgaattctct caccctgtgt aagtagcctg gtgttcgttt
     3841 gtccaagtgt acttaccctt gagaacaagt cttgcagttg ccaggtgtgt gcagacgcct
     3901 ctcctgtgag cggcatcgcg ggcccagtca gctgccccgg ctctcatcct cgtcctcact
     3961 gccttggctg agcactgttg acgtgaacag ccactgcaac aggcagccca tcccactgtc
     4021 ccctctgccc tggactccgc atggcctcca aaagccagct tgccctccgg gtgctgcagg
     4081 ggctttgcag gtggggaagg ctctggtggg tgcctttggt ggcttccgtg acatcagccg
     4141 tgctctgatg caggcttgct ccctcgtcat tctccctcgt cattctccct catcgttctg
     4201 tgtgcatttt tctctatgtc ctgacctttc ttcttgcacc taaaagtggt gtctgggtgc
     4261 aaatgtaagc agtggtggat ccagaaatat gagctttgtg taataagctt cttaaaacag
     4321 tcctttctta cacctgccca gggccagacc ctcggtcttg gcctttttca ggagctagga
     4381 tatctgcgcc tggtgaaaat gaaacgaatt cctgatttag gaattcagtg tttcctcctc
     4441 actccagcta agcactcact tttttttttt tttttttttt tttttgatac agagtctcgc
     4501 tctgtcaccc aggctggagt gcagtggtgc gatctcggct cactgcaagc tccacctccc
     4561 gggttcaagc gattcttctg cctcagcctc ctgagtaatt gggattacag gcatgcgcca
     4621 ccatgcccag ctaatttttt tttttttttt tgagacagag tcttgctctg tctcccaggc
     4681 tggagtgcaa tggcgtgatc tcggctcact gccagctccg cttcctgggt tcacgccatt
     4741 ctcctgcctc agcctcccga gtagccagga ctacaagcac ccgccaccac acctggctaa
     4801 tttttttgta tttttattag agacagtgtt ttaccgtgtt agccaggatg gtctcgatct
     4861 cctgacttcg tgatctactc acctcggcct cccaaagtgc tgggattacg ggcaagtttt
     4921 tattagagat ggggtttcat catgttggtc aggctggtct tcaactcctg acctcaggtg
     4981 atccacccgc ctcagcctcc caaagtgctg ggattacagg catgagccac cgcgcccggc
     5041 ctgaactctc agttctaacg cagggattca ggaattgggc tttcagactc cttctgcagt
     5101 gtcacagtcc agactttttt taaatgaagg acaccccgca gtggctcaca gtgggtccca
     5161 gggctagcag gagcgtgctg gggagccggc tctgtctttg ttcgcagtgg gtcccggtgc
     5221 tggcaggagt gtgctggtga gccggctctg tctttgtaaa tccttcaggg gtcctacggc
     5281 tgactccacc ggacagcccg tcctgggccg tgttaagcac cttttgtaga aatcgtattt
     5341 ttattaaaac atcaaatctg tgttcctgta a
//
