LOCUS       NM_001199050             931 bp    mRNA    linear   PRI 23-APR-2024
DEFINITION  Homo sapiens LEM domain containing 1 (LEMD1), transcript variant 1,
            mRNA.
ACCESSION   NM_001199050
VERSION     NM_001199050.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Li,L. and Zhang,P.
  TITLE     Elevation of LEM Domain Containing 1 Predicts Poor Prognosis of
            NSCLC Patients and Triggers Malignant Stemness and Invasion of
            NSCLC Cells by Stimulating PI3K/AKT Pathway
  JOURNAL   Curr Mol Med 24 (3), 366-378 (2024)
   PUBMED   36967459
  REMARK    GeneRIF: Elevation of LEM Domain Containing 1 Predicts Poor
            Prognosis of NSCLC Patients and Triggers Malignant Stemness and
            Invasion of NSCLC Cells by Stimulating PI3K/AKT Pathway.
REFERENCE   2  (bases 1 to 931)
  AUTHORS   Li,Z., Wang,J., Wu,J., Li,N. and Jiang,C.
  TITLE     Long Noncoding RNA LEMD1-AS1 Increases LEMD1 Expression and
            Activates PI3K-AKT Pathway to Promote Metastasis in Oral Squamous
            Cell Carcinoma
  JOURNAL   Biomed Res Int 2022, 3543948 (2022)
   PUBMED   35983249
  REMARK    GeneRIF: Long Noncoding RNA LEMD1-AS1 Increases LEMD1 Expression
            and Activates PI3K-AKT Pathway to Promote Metastasis in Oral
            Squamous Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 931)
  AUTHORS   Li,D., Wang,D., Liu,H. and Jiang,X.
  TITLE     LEM domain containing 1 (LEMD1) transcriptionally activated by
            SRY-related high-mobility-group box 4 (SOX4) accelerates the
            progression of colon cancer by upregulating phosphatidylinositol
            3-kinase (PI3K)/protein kinase B (Akt) signaling pathway
  JOURNAL   Bioengineered 13 (4), 8087-8100 (2022)
   PUBMED   35294319
  REMARK    GeneRIF: LEM domain containing 1 (LEMD1) transcriptionally
            activated by SRY-related high-mobility-group box 4 (SOX4)
            accelerates the progression of colon cancer by upregulating
            phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)
            signaling pathway.
REFERENCE   4  (bases 1 to 931)
  AUTHORS   Luo,Z., Hao,S., Yuan,J., Zhu,K., Liu,S., Zhang,J. and Yao,L.
  TITLE     Long non-coding RNA LINC00958 promotes colorectal cancer
            progression by enhancing the expression of LEM domain containing 1
            via microRNA miR-3064-5p
  JOURNAL   Bioengineered 12 (1), 8100-8115 (2021)
   PUBMED   34672237
  REMARK    GeneRIF: Long non-coding RNA LINC00958 promotes colorectal cancer
            progression by enhancing the expression of LEM domain containing 1
            via microRNA miR-3064-5p.
REFERENCE   5  (bases 1 to 931)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 931)
  AUTHORS   Li,Q., Ge,Y., Chen,X., Wang,L., Xia,Y., Xu,Z., Li,Z., Wang,W.,
            Yang,L., Zhang,D. and Xu,Z.
  TITLE     LEM domain containing 1 promotes proliferation via activating the
            PI3K/Akt signaling pathway in gastric cancer
  JOURNAL   J Cell Biochem 120 (9), 15190-15201 (2019)
   PUBMED   31021450
  REMARK    GeneRIF: LEM domain containing 1 promotes proliferation via
            activating the PI3K/Akt signaling pathway in gastric cancer.
REFERENCE   7  (bases 1 to 931)
  AUTHORS   Takeda,R., Hirohashi,Y., Shen,M., Wang,L., Ogawa,T., Murai,A.,
            Yamamoto,E., Kubo,T., Nakatsugawa,M., Kanaseki,T., Tsukahara,T.,
            Nishidate,T., Okita,K., Kutomi,G., Sato,N., Takemasa,I. and
            Torigoe,T.
  TITLE     Identification and functional analysis of variants of a
            cancer/testis antigen LEMD1 in colorectal cancer stem-like cells
  JOURNAL   Biochem Biophys Res Commun 485 (3), 651-657 (2017)
   PUBMED   28219643
  REMARK    GeneRIF: Data show that LEM domain containing 1 (LEMD1)has five
            transcript splicing variants (variant 1 [V1] - variant 5 [V5]), and
            that LEMD1 V1, V2 and V3 is expressed in testis and CR-CSCs/CICs,
            whereas LEMD1 V4 and V5 is ubiquitously expressed.
REFERENCE   8  (bases 1 to 931)
  AUTHORS   Sasahira,T., Kurihara,M., Nakashima,C., Kirita,T. and Kuniyasu,H.
  TITLE     LEM domain containing 1 promotes oral squamous cell carcinoma
            invasion and endothelial transmigration
  JOURNAL   Br J Cancer 115 (1), 52-58 (2016)
   PUBMED   27280633
  REMARK    GeneRIF: Downregulation of LEMD1 expression inhibited adhesion and
            transmigration of OSCCs.
REFERENCE   9  (bases 1 to 931)
  AUTHORS   Ghafouri-Fard,S., Ousati Ashtiani,Z., Sabah Golian,B.,
            Hasheminasab,S.M. and Modarressi,M.H.
  TITLE     Expression of two testis-specific genes, SPATA19 and LEMD1, in
            prostate cancer
  JOURNAL   Arch Med Res 41 (3), 195-200 (2010)
   PUBMED   20682177
  REMARK    GeneRIF: Of the prostate cancer samples, 10, 10, 23 and 40% showed
            ODF1, ODF2, LEMD1 and SPATA19 specific bands, respectively, but
            none of the BPH samples expressed any of these genes.
REFERENCE   10 (bases 1 to 931)
  AUTHORS   Yuki,D., Lin,Y.M., Fujii,Y., Nakamura,Y. and Furukawa,Y.
  TITLE     Isolation of LEM domain-containing 1, a novel testis-specific gene
            expressed in colorectal cancers
  JOURNAL   Oncol Rep 12 (2), 275-280 (2004)
   PUBMED   15254688
  REMARK    GeneRIF: LEM domain-containing 1 is a novel testis-specific gene
            expressed in colorectal cancers
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC399934.1, AB096677.1 and AI923802.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001199050.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036636.2, SRR5189667.303178.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367153.9/ ENSP00000356121.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DC399934.1         33-35
            4-924               AB096677.1         1-921
            925-931             AI923802.1         7-13                c
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..931
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /note="LEM domain containing 1"
                     /db_xref="GeneID:93273"
                     /db_xref="HGNC:HGNC:18725"
                     /db_xref="MIM:610480"
     exon            1..67
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            68..187
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..651
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cancer/testis antigen 50; LEM domain-containing protein 1;
                     LEM domain protein 1"
                     /codon_start=1
                     /product="LEM domain-containing protein 1 isoform 1"
                     /protein_id="NP_001185979.1"
                     /db_xref="CCDS:CCDS55679.1"
                     /db_xref="GeneID:93273"
                     /db_xref="HGNC:HGNC:18725"
                     /db_xref="MIM:610480"
                     /translation="MVDVKCLSDCKLQNQLEKLGFSPGPILPSTRKLYEKKLVQLLVS
                     PPCAPPVMNGPRELDGAQDSDDSEELNIILQGNIILSTEKSKKLKKWPEASTTKRKAV
                     DTYCLDYKPSKGRRWAARAPSTRITYGTITKERDYCAEDQTIESWREEGFPVGLKLAV
                     LGIFIIVVFVYLTVENKSLFG"
     exon            188..310
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            311..375
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            376..452
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..931
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      889..894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /note="hexamer: ATTAAA"
     polyA_site      931
                     /gene="LEMD1"
                     /gene_synonym="CT50; LEMP-1"
                     /note="major polyA site"
ORIGIN      
        1 agagtgaaac tcacccagct ttagtaacca actcgattgc atagacttta gataaccatg
       61 tgaaggggat tctaccatca gaaaagaggc caaacttcta tcatcatggt ggatgtgaag
      121 tgtctgagtg actgtaaatt gcagaaccaa cttgagaagc ttggattttc acctggccca
      181 atactacctt ccaccagaaa gttgtatgaa aaaaagttag tacagttgtt ggtctcacct
      241 ccctgtgcac cacctgtgat gaatggaccc agagagctgg atggagcgca ggacagtgat
      301 gacagcgaag agcttaatat cattttgcaa ggaaatatca tactctcaac agaaaaaagc
      361 aagaaactca aaaaatggcc tgaggcttcc accactaaac gcaaagctgt agatacctat
      421 tgcttggatt ataagccttc caagggaaga aggtgggctg caagagcacc aagcaccaga
      481 atcacatatg ggactatcac caaagagaga gactactgcg cggaagacca gactatcgag
      541 agctggagag aagaaggttt cccagtgggc ttgaagcttg ctgtgcttgg tattttcatc
      601 attgtggtgt ttgtctacct gactgtggaa aataagtcgc tgtttggtta agtaatttag
      661 gagcaaagca atgctccaag cgaggcctcc tgcttcagga aagaaccaaa acactaccct
      721 gaagggccag cctagcctgc agccctccct tgcagggagc cttcccttgc actgtgctgc
      781 tctcacagat cggtgtctgg gctcagccag gtggaaggaa cctgcctaac caggcacctg
      841 tgttaagagc atgatggtta ggaaatcccc caagtcatgt caactctcat taaaggtgct
      901 tccatatttg agcaggcgtc aaacaaggaa a
//
