LOCUS       NM_001301771            1717 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens lipid droplet assembly factor 1 (LDAF1), transcript
            variant 4, mRNA.
ACCESSION   NM_001301771 XM_005255440
VERSION     NM_001301771.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Chung,J., Wu,X., Lambert,T.J., Lai,Z.W., Walther,T.C. and
            Farese,R.V. Jr.
  TITLE     LDAF1 and Seipin Form a Lipid Droplet Assembly Complex
  JOURNAL   Dev Cell 51 (5), 551-563 (2019)
   PUBMED   31708432
  REMARK    GeneRIF: The LDAF1binds seipin and determines the sites of lipid
            droplet formation in the ER.  LDAF1-seipin complex is the core
            protein machinery that facilitates LD biogenesis.
REFERENCE   3  (bases 1 to 1717)
  AUTHORS   Castro,I.G., Eisenberg-Bord,M., Persiani,E., Rochford,J.J.,
            Schuldiner,M. and Bohnert,M.
  TITLE     Promethin Is a Conserved Seipin Partner Protein
  JOURNAL   Cells 8 (3), 268 (2019)
   PUBMED   30901948
  REMARK    GeneRIF: promethin is indeed an lipid droplet-associated protein
            that forms a complex with seipin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1717)
  AUTHORS   Eisenberg-Bord,M., Mari,M., Weill,U., Rosenfeld-Gur,E.,
            Moldavski,O., Castro,I.G., Soni,K.G., Harpaz,N., Levine,T.P.,
            Futerman,A.H., Reggiori,F., Bankaitis,V.A., Schuldiner,M. and
            Bohnert,M.
  TITLE     Identification of seipin-linked factors that act as determinants of
            a lipid droplet subpopulation
  JOURNAL   J Cell Biol 217 (1), 269-282 (2018)
   PUBMED   29187527
REFERENCE   5  (bases 1 to 1717)
  AUTHORS   Zhong,Q., Pevzner,S.J., Hao,T., Wang,Y., Mosca,R., Menche,J.,
            Taipale,M., Tasan,M., Fan,C., Yang,X., Haley,P., Murray,R.R.,
            Mer,F., Gebreab,F., Tam,S., MacWilliams,A., Dricot,A., Reichert,P.,
            Santhanam,B., Ghamsari,L., Calderwood,M.A., Rolland,T.,
            Charloteaux,B., Lindquist,S., Barabasi,A.L., Hill,D.E., Aloy,P.,
            Cusick,M.E., Xia,Y., Roth,F.P. and Vidal,M.
  TITLE     An inter-species protein-protein interaction network across vast
            evolutionary distance
  JOURNAL   Mol Syst Biol 12 (4), 865 (2016)
   PUBMED   27107014
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1717)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 1717)
  AUTHORS   Toulza,E., Mattiuzzo,N.R., Galliano,M.F., Jonca,N., Dossat,C.,
            Jacob,D., de Daruvar,A., Wincker,P., Serre,G. and Guerrin,M.
  TITLE     Large-scale identification of human genes implicated in epidermal
            barrier function
  JOURNAL   Genome Biol 8 (6), R107 (2007)
   PUBMED   17562024
REFERENCE   8  (bases 1 to 1717)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   9  (bases 1 to 1717)
  AUTHORS   Yu,S., Viswakarma,N., Batra,S.K., Sambasiva Rao,M. and Reddy,J.K.
  TITLE     Identification of promethin and PGLP as two novel up-regulated
            genes in PPARgamma1-induced adipogenic mouse liver
  JOURNAL   Biochimie 86 (11), 743-761 (2004)
   PUBMED   15589683
REFERENCE   10 (bases 1 to 1717)
  AUTHORS   Yu,S., Matsusue,K., Kashireddy,P., Cao,W.Q., Yeldandi,V.,
            Yeldandi,A.V., Rao,M.S., Gonzalez,F.J. and Reddy,J.K.
  TITLE     Adipocyte-specific gene expression and adipogenic steatosis in the
            mouse liver due to peroxisome proliferator-activated receptor
            gamma1 (PPARgamma1) overexpression
  JOURNAL   J Biol Chem 278 (1), 498-505 (2003)
   PUBMED   12401792
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF070596.1, BC015812.1, BX647512.1, CU448562.1, AL694739.1 and
            AF001550.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001301771.1.
            
            Transcript Variant: This variant (4) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. The encoded
            isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 all
            encode the same isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.140203.1,
                                           SRR7346977.621007.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000233047.9/ ENSP00000233047.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AF070596.1         298-356
            60-632              BC015812.1         1-573
            633-633             BX647512.1         522-522
            634-1348            BC015812.1         575-1289
            1349-1672           CU448562.1         38-361              c
            1673-1714           AL694739.1         37-78               c
            1715-1715           AF001550.1         48039-48039
            1716-1717           AL694739.1         34-35               c
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.3"
     gene            1..1717
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /note="lipid droplet assembly factor 1"
                     /db_xref="GeneID:57146"
                     /db_xref="HGNC:HGNC:30136"
                     /db_xref="MIM:611304"
     exon            1..73
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /inference="alignment:Splign:2.1.0"
     exon            74..267
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..657
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /note="isoform 2 is encoded by transcript variant 4;
                     PROMETHIN; transmembrane protein 159"
                     /codon_start=1
                     /product="lipid droplet assembly factor 1 isoform 2"
                     /protein_id="NP_001288700.1"
                     /db_xref="CCDS:CCDS10595.1"
                     /db_xref="GeneID:57146"
                     /db_xref="HGNC:HGNC:30136"
                     /db_xref="MIM:611304"
                     /translation="MAKEEPQSISRDLQELQKKLSLLIDSFQNNSKVVAFMKSPVGQY
                     LDSHPFLAFTLLVFIVMSAVPVGFFLLIVVLTTLAALLGVIILEGLVISVGGFSLLCI
                     LCGLGFVSLAMSGMMIASYVVVSSLISCWFSPRPLTQQNTSCDFLPAMKSAEFEGLYQ
                     E"
     exon            268..436
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /inference="alignment:Splign:2.1.0"
     exon            437..575
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /inference="alignment:Splign:2.1.0"
     exon            576..1717
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1686..1691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /note="hexamer: AATAAA"
     polyA_site      1717
                     /gene="LDAF1"
                     /gene_synonym="TMEM159"
                     /note="major polyA site"
ORIGIN      
        1 ctcttgttcc tcctcctgcc tctcttcgct tcgcctgcaa acgcggtggg ggctgctcgg
       61 cggtcaggag cagcaagaga cagagcgaca tgagagattg gaccgcgggc tgcactggag
      121 aatttactgg taggataatt catccctaaa gagattgaag tgagcttcag aatggcaaaa
      181 gaggagcccc agagtatctc aagggacttg caggaactgc agaagaagct gtctctgctg
      241 atagactcct tccagaataa ctcaaaggtg gtggccttta tgaagtctcc agtgggtcag
      301 tacttggaca gccatccgtt tctggccttc accttgctgg tgttcattgt catgtcggcc
      361 gttcctgttg gattcttcct gctcatcgtg gtgcttacca ccctggctgc tctgctgggg
      421 gtcataatat tggaaggatt ggtcatctct gtgggtggct tctcactgct ctgcatcctc
      481 tgtggtttgg gcttcgtatc actcgccatg tcggggatga tgatagcatc ttatgtagtg
      541 gtctccagcc tcatcagctg ctggttttct cccaggccac tgacacagca aaacaccagt
      601 tgtgactttc tgccagccat gaagtctgca gaattcgagg ggctttacca ggaatgagtg
      661 actgctcaga ggccgggctt cttttcaagt actgctggat catactcacc ccttgggatt
      721 atggcttaga agaagggggc tgggtaggca agcactcctt ggctgtgtcc tctcgctttt
      781 tcacttactt gtaggatccc gcagcagcca atttagggat tgtgttgttc ttgtgttggt
      841 tcccatgtaa agaagcagca gagaaatgcg atggttcaac agctcgccct gccccaagta
      901 tgcagacttg accttggcgg ggtcctgggc ttctagagtc tagcccgttg accccaaagc
      961 ctcagggctt atgtccaacg gtcccattgg gagcaacagt gattgtttat agttttttgt
     1021 tttcagaaat gagggcagct gttaattttt tcactcatgt gaaacaaaat gaaaaaaaaa
     1081 atccaaaaca gaacaagccc ttctgtggta tttgcttttt atcagaaaga acagcaacat
     1141 ttggcctctc caagttggaa aatagagtcc aaatgtaact ttgtggccaa gagtattttc
     1201 aaaaaagtct aaaggtgtag tttccactgt aactgttgag tactgttaag tactgttaat
     1261 cttttacata tttgcacttg gtactgttgt attgttctaa agagcttgtc ctttaagtaa
     1321 tttctttctt tttttttttt tttttttttt gtgacggagt ttccctcttg ttgcccaggc
     1381 tggagtgcaa tggtgcagtc tcggctcact gcaacctcca actcccaggt tcaagtgagt
     1441 ctcgtgcctc agcctcccaa gtagctggga ttacaggcac ccgccaccgt gcccggctag
     1501 tttttttttg tatttttttt agtacagatg gggtttcacc atgttggtca ggctagtctc
     1561 aaactcctga cctcaggtgc tccacctgcc ttggcctccc aaagtgctgg gattacaggc
     1621 gtgagccact gtgcccagcc taagccattt cttaaaataa aaatgctaaa ggactagtaa
     1681 gtaaaaataa aacttcctat gggatttccc agtggaa
//
