LOCUS       NM_207338               2826 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens lactase like (LCTL), transcript variant 1, mRNA.
ACCESSION   NM_207338
VERSION     NM_207338.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Kim,J., Eskiocak,U., Stadler,G., Lou,Z., Kuro-o,M., Shay,J.W. and
            Wright,W.E.
  TITLE     Short hairpin RNA screen indicates that Klotho beta/FGF19 protein
            overcomes stasis in human colonic epithelial cells
  JOURNAL   J Biol Chem 286 (50), 43294-43300 (2011)
   PUBMED   22020932
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Ito,S., Fujimori,T., Hayashizaki,Y. and Nabeshima,Y.
  TITLE     Identification of a novel mouse membrane-bound family 1
            glycosidase-like protein, which carries an atypical active site
            structure
  JOURNAL   Biochim Biophys Acta 1576 (3), 341-345 (2002)
   PUBMED   12084582
  REMARK    GeneRIF: The authors identified and cloned the mouse lactase-like
            gene (also known as Lctl, Klph). It is a member of the family 1
            glycosidases but lacks one of the conserved glutamic acid residues
            important for catalysis. Its function remains to be determined.
REFERENCE   3  (bases 1 to 2826)
  AUTHORS   Mian,I.S.
  TITLE     Sequence, structural, functional, and phylogenetic analyses of
            three glycosidase families
  JOURNAL   Blood Cells Mol Dis 24 (2), 83-100 (1998)
   PUBMED   9779294
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC116913.6.
            
            On Aug 8, 2019 this sequence version replaced NM_207338.3.
            
            Summary: This gene encodes a member of family 1 glycosidases.
            Glycosidases are enzymes that hydrolyze glycosidic bonds and are
            classified into families based on primary amino acid sequence. Most
            members of family 1 have two conserved glutamic acid residues,
            which are required for enzymatic activity. The mouse ortholog of
            this protein has been characterized and has a domain structure of
            an N-terminal signal peptide, glycosidase domain, transmembrane
            domain, and a short cytoplasmic tail. It lacks one of the conserved
            glutamic acid residues important for catalysis, and its function
            remains to be determined (PMID: 12084582). Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jun
            2013].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.68360.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1968540,
                                           SAMEA1968968 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000695545.1/ ENSP00000512002.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-142               AC116913.6         136525-136666       c
            143-282             AC116913.6         135916-136055       c
            283-446             AC116913.6         135344-135507       c
            447-534             AC116913.6         134579-134666       c
            535-644             AC116913.6         134184-134293       c
            645-773             AC116913.6         131855-131983       c
            774-869             AC116913.6         131674-131769       c
            870-924             AC116913.6         128650-128704       c
            925-1086            AC116913.6         128390-128551       c
            1087-1361           AC116913.6         123652-123926       c
            1362-1488           AC116913.6         122711-122837       c
            1489-1688           AC116913.6         122330-122529       c
            1689-1752           AC116913.6         120709-120772       c
            1753-2826           AC116913.6         118200-119273       c
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..2826
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /note="lactase like"
                     /db_xref="GeneID:197021"
                     /db_xref="HGNC:HGNC:15583"
                     /db_xref="MIM:617060"
     exon            1..142
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            143..282
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..1868
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; klotho gamma; KL lactase phlorizin hydrolase;
                     lactase-like protein; klotho/lactase-phlorizin
                     hydrolase-related protein; gammaklotho"
                     /codon_start=1
                     /product="lactase-like protein isoform 1 precursor"
                     /protein_id="NP_997221.2"
                     /db_xref="CCDS:CCDS10220.1"
                     /db_xref="GeneID:197021"
                     /db_xref="HGNC:HGNC:15583"
                     /db_xref="MIM:617060"
                     /translation="MKPVWVATLLWMLLLVPRLGAARKGSPEEASFYYGTFPLGFSWG
                     VGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVACDGYYKVQEDIILLRE
                     LHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSNITPIVTLHHWDLPQ
                     LLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGYETGHHAPG
                     LKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDL
                     EAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKG
                     TSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFR
                     RLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIK
                     GYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPRE
                     VESWYLKALETCSINNQMLAAEPLLSHMQMVTEIVVPTVCSLCVLITAVLLMLLLRRQ
                     S"
     exon            283..446
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            447..534
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            535..644
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            645..773
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            774..869
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            870..924
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1086
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1361
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1488
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1688
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1752
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..2826
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2110..2115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /note="hexamer: AATGAA"
     polyA_site      2156
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
     regulatory      2803..2808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /note="hexamer: AATAAA"
     polyA_site      2826
                     /gene="LCTL"
                     /gene_synonym="KLG; KLPH"
                     /note="major polyA site"
ORIGIN      
        1 acagaccaca cccccaacca gagccttcgg ctgtgctgtg gctcctctct caggggacag
       61 cgtaggccct gggggtcagt tggagagggc tctgactacc agcgactgct ctgggggtgt
      121 ctgcgatcaa ggacgatcct gggtatgggg gagggccagg caccatgaag ccagtgtggg
      181 tcgccaccct tctgtggatg ctactgctgg tgcccaggct gggggccgcc cggaaggggt
      241 ccccagaaga ggcctccttc tactatggaa ccttccctct tggcttctcc tggggcgtgg
      301 gcagttctgc ctaccagacg gagggcgcct gggaccagga cgggaaaggg cctagcatct
      361 gggacgtctt cacacacagt gggaagggga aagtgcttgg gaatgagacg gcagatgtag
      421 cctgtgacgg ctactacaag gtccaggagg acatcattct gctgagggaa ctgcacgtca
      481 accactaccg attctccctg tcttggcccc ggctcctgcc cacaggcatc cgagccgagc
      541 aggtgaacaa gaagggaatc gaattctaca gtgatcttat cgatgccctt ctgagcagca
      601 acatcactcc catcgtgacc ttgcaccact gggatctgcc acagctgctc caggtcaaat
      661 acggtgggtg gcagaatgtg agcatggcca actacttcag agactacgcc aacctgtgct
      721 ttgaggcctt tggggaccgt gtgaagcact ggatcacgtt cagtgatcct cgggcaatgg
      781 cagaaaaagg ctatgagacg ggccaccatg cgccgggcct gaagctccgc ggcaccggcc
      841 tgtacaaggc agcacaccac atcattaagg cccacgccaa agcctggcat tcttataaca
      901 ccacgtggcg cagcaagcag caaggtctgg tgggaatttc attgaactgt gactgggggg
      961 aacctgtgga cattagtaac cccaaggacc tagaggctgc cgagagatac ctacagttct
     1021 gtctgggctg gtttgccaac cccatttatg ccggtgacta cccccaagtc atgaaggact
     1081 acattggaag aaagagtgca gagcaaggcc tggagatgtc gaggttaccg gtgttctcac
     1141 tccaggagaa gagctacatt aaaggcacat ccgatttctt gggattaggt cattttacta
     1201 ctcggtacat cacggaaagg aactacccct cccgccaggg gcccagctac cagaacgatc
     1261 gtgacttgat agagctggtt gacccaaact ggccagatct ggggtctaaa tggctatatt
     1321 ctgtgccatg gggatttagg aggctcctta actttgctca gactcaatac ggtgatcctc
     1381 ccatatatgt gatggaaaat ggagcatctc aaaaattcca ctgtactcaa ttatgtgatg
     1441 agtggagaat tcaatacctt aaaggataca taaatgaaat gctaaaagct ataaaagatg
     1501 gtgctaatat aaaggggtat acttcctggt ctctgttgga taagtttgaa tgggagaaag
     1561 gatactcaga tagatatgga ttctactatg ttgaatttaa cgacagaaat aagcctcgct
     1621 atccaaaggc ttcagttcaa tattacaaga agattatcat tgccaatggg tttcccaatc
     1681 caagagaggt ggaaagttgg tacctcaaag ctttggaaac ttgctctatc aacaatcaga
     1741 tgcttgctgc agagcccttg ctaagtcaca tgcaaatggt tacggagatc gtggtaccca
     1801 ctgtctgctc cctctgtgtc ctcatcactg ctgttctact aatgctcctc ctgaggaggc
     1861 agagctgaga caggattatc aattttggag cttcataaga gaatcttcag gatcttcctc
     1921 ccttttctgc tttgagggtt tccatacatt gctgttttca ggttctacaa taattacctt
     1981 tttttctctt tctctttttg gcttgtgctg ggatttaaga attagaaaat aaaaataagc
     2041 agaaattaaa aaaaaaaaca cttgtaatct cacccctcct aaagttaatg ttaagatttt
     2101 gctgtatata atgaatgtat attatgaatt atttacaaaa atggcatcat agtatacaaa
     2161 ctgttgtgac ttgattgttt atcactctaa aatctgtcca gaaatattac tttaatgttc
     2221 aaatgattgc catctcagca ctgtcccata cctgatagat aaaatcaatc tggctgggca
     2281 cagtggctca cacctgtaat cccagcactt tgggaggctg aggctggtga atcacttgag
     2341 gccagaagtt tgagaccagc ctggccaaca tggttaaacc ctgtctctac taaaaatata
     2401 gaaactagct gggcatggtg gcgggcacct gtaatcccat ttacttggga ggctgaggca
     2461 ggagaatcgc ttgaacctgg gaggtgaagg ttgcagtgag ctgagatcac accactgcac
     2521 tccagcctgt gcaacagaga aagactccgt ttcaaaagag aaaagaaaag aaatctatag
     2581 ataacaatcc ttccaggttg taaatattat tagccaacca cagaaaagca tgaagtgggt
     2641 accaagtaaa tgagcacgta cactcttctg acactttagt ctgaatttag tctgacaact
     2701 ctgactaaat tcaacttact ttagtcaatg aaatacctca aagaaatatt acatgtttat
     2761 tttttattta tcttctaggt aactcaatat cgtctttttt ataataaaga ttcattttac
     2821 tcttaa
//
