LOCUS       NM_014793               6925 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA.
ACCESSION   NM_014793
VERSION     NM_014793.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Jeon,B.N., Kim,M.K., Choi,W.I., Koh,D.I., Hong,S.Y., Kim,K.S.,
            Kim,M., Yun,C.O., Yoon,J., Choi,K.Y., Lee,K.R., Nephew,K.P. and
            Hur,M.W.
  TITLE     KR-POK interacts with p53 and represses its ability to activate
            transcription of p21WAF1/CDKN1A
  JOURNAL   Cancer Res 72 (5), 1137-1148 (2012)
   PUBMED   22253232
  REMARK    GeneRIF: Kr-pok interacted directly with the DNA binding and
            oligomerization domains of p53. This interaction was essential for
            Kr-pok to bind the distal promoter region of the CDKN1A gene,
            p21WAF1, and to inhibit p53-mediated transcriptional activation of
            CDKN1A.
REFERENCE   3  (bases 1 to 6925)
  AUTHORS   Suzuki,Y., Noma,A., Suzuki,T., Ishitani,R. and Nureki,O.
  TITLE     Structural basis of tRNA modification with CO2 fixation and
            methylation by wybutosine synthesizing enzyme TYW4
  JOURNAL   Nucleic Acids Res 37 (9), 2910-2925 (2009)
   PUBMED   19287006
  REMARK    GeneRIF: This paper reports crystal structures of a yeast homolog,
            TYW4.
REFERENCE   4  (bases 1 to 6925)
  AUTHORS   Dennis,J.H., Fan,H.Y., Reynolds,S.M., Yuan,G., Meldrim,J.C.,
            Richter,D.J., Peterson,D.G., Rando,O.J., Noble,W.S. and
            Kingston,R.E.
  TITLE     Independent and complementary methods for large-scale structural
            analysis of mammalian chromatin
  JOURNAL   Genome Res 17 (6), 928-939 (2007)
   PUBMED   17568008
REFERENCE   5  (bases 1 to 6925)
  AUTHORS   Noma,A., Kirino,Y., Ikeuchi,Y. and Suzuki,T.
  TITLE     Biosynthesis of wybutosine, a hyper-modified nucleoside in
            eukaryotic phenylalanine tRNA
  JOURNAL   EMBO J 25 (10), 2142-2154 (2006)
   PUBMED   16642040
REFERENCE   6  (bases 1 to 6925)
  AUTHORS   De Baere,I., Derua,R., Janssens,V., Van Hoof,C., Waelkens,E.,
            Merlevede,W. and Goris,J.
  TITLE     Purification of porcine brain protein phosphatase 2A leucine
            carboxyl methyltransferase and cloning of the human homologue
  JOURNAL   Biochemistry 38 (50), 16539-16547 (1999)
   PUBMED   10600115
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC015949.2 and AC009852.9.
            
            On May 17, 2019 this sequence version replaced NM_014793.4.
            
            Summary: The protein encoded by this intronless gene belongs to the
            highly variable methyltransferase superfamily. This gene is the
            inferred homolog of the Saccharomyces cerevisiae
            carboxymethyltransferase gene PPM2 that is essential for the
            synthesis of the hypermodified guanosine Wybutosine (yW). [provided
            by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR1803613.31183.1, SRR1803616.44518.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305641.7/ ENSP00000307214.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2798              BC015949.2         33-2830
            2799-6925           AC009852.9         157441-161567       c
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..6925
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="leucine carboxyl methyltransferase 2"
                     /db_xref="GeneID:9836"
                     /db_xref="HGNC:HGNC:17558"
                     /db_xref="MIM:611246"
     exon            1..6925
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..2145
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /EC_number="2.1.1.290"
                     /EC_number="2.3.1.231"
                     /note="tRNA-Wybutosine-synthesizing protein 4;
                     p21WAF1/CIP1 promoter-interacting protein; tRNA(Phe)
                     (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-
                     methyltransferase; tRNA(Phe)
                     (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-
                     methoxycarbonyltransferase; tRNA-yW synthesizing protein
                     4"
                     /codon_start=1
                     /product="tRNA wybutosine-synthesizing protein 4"
                     /protein_id="NP_055608.2"
                     /db_xref="CCDS:CCDS10094.1"
                     /db_xref="GeneID:9836"
                     /db_xref="HGNC:HGNC:17558"
                     /db_xref="MIM:611246"
                     /translation="MGPRSRERRAGAVQNTNDSSALSKRSLAARGYVQDPFAALLVPG
                     AARRAPLIHRGYYVRARAVRHCVRAFLEQIGAPQAALRAQILSLGAGFDSLYFRLKTA
                     GRLARAAVWEVDFPDVARRKAERIGETPELCALTGPFERGEPASALCFESADYCILGL
                     DLRQLQRVEEALGAAGLDAASPTLLLAEAVLTYLEPESAAALIAWAAQRFPNALFVVY
                     EQMRPQDAFGQFMLQHFRQLNSPLHGLERFPDVEAQRRRFLQAGWTACGAVDMNEFYH
                     CFLPAEERRRVENIEPFDEFEEWHLKCAHYFILAASRGDTLSHTLVFPSSEAFPRVNP
                     ASPSGVFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLL
                     SRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLH
                     FFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVL
                     SDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLR
                     PISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLS
                     SEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSL
                     SAGQ"
     regulatory      2773..2778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="hexamer: AATAAA"
     polyA_site      2798
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="major polyA site"
     regulatory      3236..3241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="hexamer: AATAAA"
     polyA_site      3268
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
     regulatory      6795..6800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="hexamer: ATTAAA"
     polyA_site      6817
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
     regulatory      6902..6907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
                     /note="hexamer: AATAAA"
     polyA_site      6925
                     /gene="LCMT2"
                     /gene_synonym="PPM2; TYW4"
ORIGIN      
        1 ccctaccgag gtccgtgagg tgtgtgctaa ccaaggggcg gctcacaacc gtgacagact
       61 gccattcctg agtctcttct ggccatgggc ccccggagcc gtgagcgtcg ggcaggcgcg
      121 gtacagaaca ccaacgacag cagcgccctc agcaagcgtt ccctggccgc gcgcgggtac
      181 gtgcaggacc cctttgccgc gttgctggtt ccgggcgcgg cgcgccgcgc accgctcatt
      241 caccgaggct actacgtccg cgcacgcgcc gtgaggcact gcgtgcgcgc ttttttggag
      301 cagattggcg cgccccaggc cgcgcttcgc gcgcagatct tgtctctcgg cgctggcttc
      361 gactcgctct attttcgctt aaaaaccgcg ggccgcctgg cccgggctgc agtctgggag
      421 gtggattttc cggacgtggc gcggcgcaaa gcagaaagga ttggagagac gccagagctg
      481 tgcgcgttaa ccgggccttt cgagaggggg gagcccgcgt ccgcgctgtg ctttgagagc
      541 gcagactact gcatcctggg tctggacttg cggcagctcc agcgagtgga ggaggccctg
      601 ggcgccgcgg ggctcgacgc agcctcaccc actctgctcc tggccgaggc ggtgctgacc
      661 tacctcgagc cggagagtgc cgcggccctc atcgcctggg cagcccagcg ttttcctaat
      721 gcccttttcg tggtctatga gcagatgagg cctcaagacg cctttggcca gttcatgctg
      781 caacattttc ggcagctaaa ctcccccctg catggcctgg agcgttttcc tgacgtggag
      841 gcgcagcggc gccgcttcct tcaagctggc tggaccgcct gcggtgccgt ggacatgaat
      901 gaattctatc actgctttct tcccgcagaa gaacgccggc gggtggaaaa tattgaaccc
      961 tttgacgaat ttgaggagtg gcatctgaag tgcgcccatt atttcattct ggcagcttct
     1021 aggggagaca ccctctccca caccctagtg tttccatcct cagaggcatt tcctcgcgta
     1081 aatcctgctt cgccttcagg ggtattccct gccagcgtag tcagtagcga gggccaggtc
     1141 ccaaacctga agagatatgg ccacgcctct gtcttcttga gcccagacgt tattctcagt
     1201 gcaggaggat ttggagagca ggaggggcgg cactgccgag tgagccagtt tcacttgctc
     1261 tcaagagatt gtgactctga atggaaaggc agccaaatag gcagttgtgg gactggagtt
     1321 cagtgggatg gacgccttta tcacaccatg acaagactct cagagagtcg ggttctggtt
     1381 ctgggaggga gactgtcccc agtaagtcca gccttggggg ttctccagct tcattttttt
     1441 aagagtgagg ataataacac tgaggacctg aaagtgacaa taacaaaggc tggccgaaag
     1501 gatgattcca ctttgtgttg ttggcggcat tcaacaacag aagtgtcctg tcagaatcag
     1561 gaatatttgt ttgtgtatgg gggtcgaagc gtggtggaac ctgtactaag tgactggcat
     1621 ttcctccatg tagggacaat ggcttgggtc aggatcccag tggagggaga agtacctgaa
     1681 gcccggcatt ctcacagtgc ctgcacttgg caagggggag cccttattgc tggaggtctc
     1741 ggggcttctg aggagccatt gaactctgtg ctctttctga gaccaatctc ttgtggattc
     1801 ctctgggagt cagtagacat ccagcctccc attaccccaa ggtactccca cacagctcat
     1861 gtgctcaatg gaaagctgtt actggttgga gggatctgga ttcattcctc ctcatttcct
     1921 ggagtgactg tgatcaattt gactacagga ttgagctctg agtatcagat tgacacaaca
     1981 tatgtgccat ggccattaat gttacacaac catactagta tccttcttcc tgaagagcaa
     2041 cagctcctgc tccttggagg tggtgggaac tgcttttcct ttggtaccta cttcaacccc
     2101 catacagtca cattagacct ttcttcctta agtgctgggc agtaaggact ggactaatat
     2161 tcaggaccca ctaaagtaga caataaagtt ttccacaaat aggatgaccc tctagctata
     2221 gatactgcca ctcctccttt ccccatcctt tttttccctt agcactattc agtgcaaaaa
     2281 gtgaaaaagg ttggtaaaat aggtaaaata cctagaaaca atcactacag aaaacagctg
     2341 aagacagtgg ccatgcagtc cgagaggagt agtggtctgc ctctaatttt ctaatctaag
     2401 ttcgtttatt gagttacagt ggtctttagt aaagtaaaac aatttcccaa tcccaggcct
     2461 tgtgatttga gatggtacct tagaaaaagt tacacgcagt tccgtggttg aatatatttg
     2521 agatggtacc ttagaaaaag tttcacgcag atccttggtt gaatatagtt gagggagcgt
     2581 agtattgaca attcttcatg taggaaacct gaaatgaaca cagtcacagt ttgattaaaa
     2641 cattgtcctg tttgttgcaa cagaaaactc ggatagtttt aacaacagga aacacttgta
     2701 ggacttcctt taccaacata ctttttaaat gttttgctat tggttccata tttatttaga
     2761 tttataagtg tcaataaagc aaacttttga tgcctcaatt cattttgcac ggggtataat
     2821 aatgttttgt tccagatata ctctccacct ttcactgttc tgtgctccaa gaggctgacc
     2881 tttatgggct gcatcaagga gctctcttta ctttttggtt gagttccacc agtcagaggc
     2941 accagagggg aatgggggca aaaagaagag tgagagtgag ttggtattta ttcccaattc
     3001 ttcccctgtt ggtttgcaag ttggcagtgg cagcattctt ctgtcaaagg ctacagctcc
     3061 tgccgtgtaa tcctttcctg aagacagagt tctaattctg catcatccca ggtttcagta
     3121 attgttcctt tacttcacct tttcaggcct aggagttgtg atggctcttt gctagacctg
     3181 aactgtactg tccaatgtgg tagctattga caacatgtga ctatttaaac gaattaataa
     3241 attcagtggc ttagtcacat tgaccacatt ttaagtgctt aataactatg tgtggctttg
     3301 gctactgtat tagacaatgt agatgtagaa catttcatcg ttacagaaat ttctactgga
     3361 cactgctgac tggatatctt gacttggttt cccttaaccc tactcagccc cttatagtta
     3421 gcatcccatt aaactcaatt atccctttta aatgggaatc tgttgcctag tgagccatgt
     3481 gagatcaatg aaatagctat tcatccttaa aatttgagcc ttaatttgtc atctaaatta
     3541 tgcatacctt gtgaaatata cacctcaaaa aatgtagtgg ctttggtggt aataagtgta
     3601 ttactttagt atttcttctt agtcctagaa accttagtag ttttcattct acaagtgcct
     3661 aaatgattat tatgaaccat cattgatgac aaattgcatc ctttatttct attaattttg
     3721 catataaatg gatgtgaaag gtgattaata taaaaaccaa gtagcaaaca caaacctaag
     3781 tgtatggaga ggacagtctt ggctctgtta gatgaagcct ccccggggga gaccagctga
     3841 atgagggttg tggtataggc tgggcatggt ggctcacgcc tataattcca gcactttggg
     3901 aggccaagac agaaggatca cttaaagcca ggagtttaag accagcccag gcaacaaagt
     3961 cagatcccca tctctacaaa aaaataagta ggcacggtgg tgtgtgcttg tagtcttgga
     4021 tactctggag gctgaggtga tagtgagctg tgattgtgcc actgcactcc tgcctggttg
     4081 acagagtgag accccgtctc ttaaaaagaa aaaaaaaaaa ggttgttgtc caacttcagc
     4141 tttcctgaga ctttgtaaga gccatttcta gggaaagcat tggttatggc tatgactgag
     4201 tcctgggaaa cagggcttat tacagctggg aagacatctg gcaaggcgat cagtgggcca
     4261 ggtagtagaa aactggcagg gaatatgatc agcaggagaa aatggctgtt ctgtatttcc
     4321 tgtttctaag tttactacag cacaccccag ctttcaggag cagataaaag cagaacactg
     4381 tgatgaggcc aggcgcagtg gcacatgcct tgatcccagc actttgggag gccaaggctg
     4441 gcagatcact tgaactcagg agttggagac cagcctggcc aatatggtga aactccgtct
     4501 ctacccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat tatccatcta tctatatata
     4561 caaagattag ccagggatgg tggcgcatgc ctgtagtccc agctactcag gtactgaggt
     4621 gggaggatta cttgagcctg gcaggtcgag ccaagatcat gcaactgctc tccagcttgg
     4681 gtgacagagt gagaccctgt ctctagtaat aacaacactg taatgaaatg agtgttctca
     4741 gctatgtagt agagtgtacc agaattcaaa cctagatcta actccaaagc ccaggttaca
     4801 ccttccacta tgatgctgct tacatcatgc catcagccat tatttcatta cttttggggg
     4861 gaaaagaagg gaggagggag gtcatctaga gaatgtggac gtgtgccaga cctaaattag
     4921 gagtgcaata gtgcctattc tcttgagcac tgtccattag ggcgccttat gagtaacagg
     4981 gcttgttctc ccgagagaaa ttacccacat ttcctataag tgattaccag attaagcatg
     5041 ccctccacct ctaactgagc atcatgctca gttaaggaat tattcatctc tcgagctcta
     5101 ctcattagag cacttagaag tattgtcagc aatatttacc ctgtgccttt ggagcagcat
     5161 tcaatataga ctagcgttga accacagttt cattaacagt aatctgcaca gttggaggtg
     5221 tgagccagat tgagaaccaa agctggataa taattcttgc ttctccattt cacttttgaa
     5281 gtttttgttc taaaattctg atacggagct ctggagtagt tatgagagtt aatgaacagg
     5341 tctcttcctc ttttagggat tacttctaga ccagataaac tacttaagga aggaaagtaa
     5401 tttcttattt tgaatctcca ctctgggtca gggtttcaaa aatagtgtat cataatagtg
     5461 aagatgaaaa caggttaaga acaactgttg gaggatatgc tcttcaaggc cagtgctgtt
     5521 gcttcttttt gtactttctg agttttaaaa ctgagtgccc gtttttcatc tttctggagc
     5581 tttaagtctt cttaatgatc tttgggagat ggtatgtatc caatatgttc caggacccag
     5641 gtgtttcctt ggtctttttc agtgatctca gaccttgaag tcctagctta ttccatatgt
     5701 attttgtgat cacaaaccca acctcaagca gcccatgctt accctgggac ttatcccaca
     5761 tgtcatctcc tggaaaacag ccctatatga ctgccccaaa tggaaccaac cgctttctcc
     5821 ctgtgcaaga gaagcctttg aagagaagct ctttgaagag tttggtgaat aaaagaacta
     5881 gttaataagg tgaggttttt tgggtttgtt tgtttgtttg tttgtttgtt tgctttttgt
     5941 tttttgtttt gagacggagt cttgctctgt tgcccaggct agagtgcagt ggctcgatct
     6001 cggctcactg caacctccac ctcctgggtt caagtgattc tcctgcctcg gcttccggag
     6061 tagctggaat tacaggtgcg caccaccatg cccggctaat ttttttttta tttattttta
     6121 gtagagatgg ggtttcacca tgttggtcag gctggtatcc aactcctgac ctcaagtgat
     6181 ctgcccgcct cggcctccca aagtgctgag attacaggcg tgagccacca tgcctggcct
     6241 aggtgggttt ttaaccatag ggacagtcca ataatgtcta gtttgacttt aagattaagc
     6301 cttttaactt cagtaatgaa ggaaggggtg taaaatagca ttaagccggc aagttaggtc
     6361 atgtaacttg gctaatcaca ttagacttga tgtttaaaga gagaaggaga gtgtgtagag
     6421 caggaattat gaaatttata atcagcttta gagtaggctg agatttcctt ggatgtcaat
     6481 aattatgagt aaacaagaat tctgtcatcc cacatgtaag taagcagtta caagactgct
     6541 tacttatctg tcttggacat tgatagaaag cacatgattg ggaaggggca gttccttgac
     6601 tgagggaagg aattgtgtct ggttcacttt tctatcacca acatagtaga tgctcaacaa
     6661 atacttgttg gatagaggtc cactcttgta ctcaaataaa ttatatttga atattactat
     6721 agttcttatg cacaaactga tgtcaataac tcctgtaatt gttgtgtatg tgtgtgtgtc
     6781 agggggaggt aagcattaaa attttaaaga acccacagtt taagagtctt tggaaataca
     6841 ttcaactttt ctgacccagg taaaggtggt agactttctg gtgtcatttc tatctttatg
     6901 gaataaacct gaaccttctc cctga
//
