LOCUS       NM_005356               2091 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens LCK proto-oncogene, Src family tyrosine kinase (LCK),
            transcript variant 2, mRNA.
ACCESSION   NM_005356
VERSION     NM_005356.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Deng,S., Zhang,Y., Wang,H., Liang,W., Xie,L., Li,N., Fang,Y.,
            Wang,Y., Liu,J., Chi,H., Sun,Y., Ye,R., Shan,L., Shi,J., Shen,Z.,
            Wang,Y., Wang,S., Brosseau,J.P., Wang,F., Liu,G., Quan,Y. and Xu,J.
  TITLE     ITPRIPL1 binds CD3epsilon to impede T cell activation and enable
            tumor immune evasion
  JOURNAL   Cell 187 (9), 2305-2323 (2024)
   PUBMED   38614099
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Jaeger-Ruckstuhl,C.A., Lo,Y., Fulton,E., Waltner,O.G.,
            Shabaneh,T.B., Simon,S., Muthuraman,P.V., Correnti,C.E.,
            Newsom,O.J., Engstrom,I.A., Kanaan,S.B., Bhise,S.S.,
            Peralta,J.M.C., Ruff,R., Price,J.P., Stull,S.M., Stevens,A.R.,
            Bugos,G., Kluesner,M.G., Voillet,V., Muhunthan,V., Morrish,F.,
            Olson,J.M., Gottardo,R., Sarthy,J.F., Henikoff,S., Sullivan,L.B.,
            Furlan,S.N. and Riddell,S.R.
  TITLE     Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell
            activation promotes memory-associated gene regulatory networks
  JOURNAL   Immunity 57 (2), 287-302 (2024)
   PUBMED   38354704
REFERENCE   3  (bases 1 to 2091)
  AUTHORS   Keller,B., Kfir-Erenfeld,S., Matusewicz,P., Hartl,F., Lev,A.,
            Lee,Y.N., Simon,A.J., Stauber,T., Elpeleg,O., Somech,R.,
            Stepensky,P., Minguet,S., Schraven,B. and Warnatz,K.
  TITLE     Combined Immunodeficiency Caused by a Novel Nonsense Mutation in
            LCK
  JOURNAL   J Clin Immunol 44 (1), 4 (2023)
   PUBMED   38112969
  REMARK    GeneRIF: Combined Immunodeficiency Caused by a Novel Nonsense
            Mutation in LCK.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2091)
  AUTHORS   Lanz,A.L., Erdem,S., Ozcan,A., Ceylaner,G., Cansever,M.,
            Ceylaner,S., Conca,R., Magg,T., Acuto,O., Latour,S., Klein,C.,
            Patiroglu,T., Unal,E., Eken,A. and Hauck,F.
  TITLE     A Novel Biallelic LCK Variant Resulting in Profound T-Cell Immune
            Deficiency and Review of the Literature
  JOURNAL   J Clin Immunol 44 (1), 1 (2023)
   PUBMED   38100037
  REMARK    GeneRIF: A Novel Biallelic LCK Variant Resulting in Profound T-Cell
            Immune Deficiency and Review of the Literature.
            Review article
            Erratum:[J Clin Immunol. 2024 Jan 16;44(1):41. doi:
            10.1007/s10875-024-01654-4.. PMID: 38225415]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2091)
  AUTHORS   Al Qutami,F., Al Halabi,W. and Hachim,M.Y.
  TITLE     Identification of Breast Cancer LCK Proto-Oncogene as a Master
            Regulator of TNBC Neutrophil Enrichment and Polarization
  JOURNAL   Int J Mol Sci 24 (17), 13269 (2023)
   PUBMED   37686072
  REMARK    GeneRIF: Identification of Breast Cancer LCK Proto-Oncogene as a
            Master Regulator of TNBC Neutrophil Enrichment and Polarization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2091)
  AUTHORS   Shenoy-Scaria,A.M., Kwong,J., Fujita,T., Olszowy,M.W., Shaw,A.S.
            and Lublin,D.M.
  TITLE     Signal transduction through decay-accelerating factor. Interaction
            of glycosyl-phosphatidylinositol anchor and protein tyrosine
            kinases p56lck and p59fyn 1
  JOURNAL   J Immunol 149 (11), 3535-3541 (1992)
   PUBMED   1385527
REFERENCE   7  (bases 1 to 2091)
  AUTHORS   Alai,M., Mui,A.L., Cutler,R.L., Bustelo,X.R., Barbacid,M. and
            Krystal,G.
  TITLE     Steel factor stimulates the tyrosine phosphorylation of the
            proto-oncogene product, p95vav, in human hemopoietic cells
  JOURNAL   J Biol Chem 267 (25), 18021-18025 (1992)
   PUBMED   1381360
REFERENCE   8  (bases 1 to 2091)
  AUTHORS   Weber,J.R., Bell,G.M., Han,M.Y., Pawson,T. and Imboden,J.B.
  TITLE     Association of the tyrosine kinase LCK with phospholipase C-gamma 1
            after stimulation of the T cell antigen receptor
  JOURNAL   J Exp Med 176 (2), 373-379 (1992)
   PUBMED   1500851
REFERENCE   9  (bases 1 to 2091)
  AUTHORS   Molina,T.J., Kishihara,K., Siderovski,D.P., van Ewijk,W.,
            Narendran,A., Timms,E., Wakeham,A., Paige,C.J., Hartmann,K.U.,
            Veillette,A. et al.
  TITLE     Profound block in thymocyte development in mice lacking p56lck
  JOURNAL   Nature 357 (6374), 161-164 (1992)
   PUBMED   1579166
REFERENCE   10 (bases 1 to 2091)
  AUTHORS   Levinson,A.D., Oppermann,H., Levintow,L., Varmus,H.E. and
            Bishop,J.M.
  TITLE     Evidence that the transforming gene of avian sarcoma virus encodes
            a protein kinase associated with a phosphoprotein
  JOURNAL   Cell 15 (2), 561-572 (1978)
   PUBMED   214242
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA007906.1, X13529.1,
            AF228313.1 and AA747421.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005356.4.
            
            Summary: This gene is a member of the Src family of protein
            tyrosine kinases (PTKs). The encoded protein is a key signaling
            molecule in the selection and maturation of developing T-cells. It
            contains N-terminal sites for myristylation and palmitylation, a
            PTK domain, and SH2 and SH3 domains which are involved in mediating
            protein-protein interactions with phosphotyrosine-containing and
            proline-rich motifs, respectively. The protein localizes to the
            plasma membrane and pericentrosomal vesicles, and binds to cell
            surface receptors, including CD4 and CD8, and other signaling
            molecules. Multiple alternatively spliced variants encoding
            different isoforms have been described. [provided by RefSeq, Aug
            2016].
            
            Transcript Variant: This variant (2) is transcribed from the distal
            type II promoter and contains a distinct 5' UTR compared to variant
            1. Variants 1 and 2 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.565415.1,
                                           SRR1163658.539310.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336890.10/ ENSP00000337825.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                DA007906.1         27-93
            68-371              X13529.1           7-310
            372-1642            AF228313.1         221-1491
            1643-2091           AA747421.1         19-467              c
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.2"
     gene            1..2091
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /note="LCK proto-oncogene, Src family tyrosine kinase"
                     /db_xref="GeneID:3932"
                     /db_xref="HGNC:HGNC:6524"
                     /db_xref="MIM:153390"
     exon            1..107
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            108..217
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1642
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /EC_number="2.7.10.2"
                     /note="isoform a is encoded by transcript variant 2;
                     T-lymphocyte specific protein tyrosine kinase p56lck;
                     p56(LSTRA) protein-tyrosine kinase; proto-oncogene
                     tyrosine-protein kinase LCK; leukocyte C-terminal Src
                     kinase; t cell-specific protein-tyrosine kinase;
                     lymphocyte cell-specific protein-tyrosine kinase"
                     /codon_start=1
                     /product="tyrosine-protein kinase Lck isoform a"
                     /protein_id="NP_005347.3"
                     /db_xref="CCDS:CCDS359.1"
                     /db_xref="GeneID:3932"
                     /db_xref="HGNC:HGNC:6524"
                     /db_xref="MIM:153390"
                     /translation="MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEV
                     RDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQS
                     LTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTA
                     GSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCT
                     RLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLK
                     QGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGI
                     KLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDN
                     EYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVI
                     QNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQ
                     P"
     exon            218..299
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            300..390
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            391..489
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            490..593
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            594..743
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            744..896
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1076
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1153
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1307
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1439
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..2091
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2065..2070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /note="hexamer: AATAAA"
     polyA_site      2091
                     /gene="LCK"
                     /gene_synonym="IMD22; LSK; p56lck; pp58lck; YT16"
                     /note="major polyA site"
ORIGIN      
        1 gagacaggtg gtggctacga cggcgaaggg agctgagact gtccaggcag ccaggttagg
       61 ccaggaggac catgtgaatg gggccagagg gctcccgggc tgggcaggga ccatgggctg
      121 tggctgcagc tcacacccgg aagatgactg gatggaaaac atcgatgtgt gtgagaactg
      181 ccattatccc atagtcccac tggatggcaa gggcacgctg ctcatccgaa atggctctga
      241 ggtgcgggac ccactggtta cctacgaagg ctccaatccg ccggcttccc cactgcaaga
      301 caacctggtt atcgctctgc acagctatga gccctctcac gacggagatc tgggctttga
      361 gaagggggaa cagctccgca tcctggagca gagcggcgag tggtggaagg cgcagtccct
      421 gaccacgggc caggaaggct tcatcccctt caattttgtg gccaaagcga acagcctgga
      481 gcccgaaccc tggttcttca agaacctgag ccgcaaggac gcggagcggc agctcctggc
      541 gcccgggaac actcacggct ccttcctcat ccgggagagc gagagcaccg cgggatcgtt
      601 ttcactgtcg gtccgggact tcgaccagaa ccagggagag gtggtgaaac attacaagat
      661 ccgtaatctg gacaacggtg gcttctacat ctcccctcga atcacttttc ccggcctgca
      721 tgaactggtc cgccattaca ccaatgcttc agatgggctg tgcacacggt tgagccgccc
      781 ctgccagacc cagaagcccc agaagccgtg gtgggaggac gagtgggagg ttcccaggga
      841 gacgctgaag ctggtggagc ggctgggggc tggacagttc ggggaggtgt ggatggggta
      901 ctacaacggg cacacgaagg tggcggtgaa gagcctgaag cagggcagca tgtccccgga
      961 cgccttcctg gccgaggcca acctcatgaa gcagctgcaa caccagcggc tggttcggct
     1021 ctacgctgtg gtcacccagg agcccatcta catcatcact gaatacatgg agaatgggag
     1081 tctagtggat tttctcaaga ccccttcagg catcaagttg accatcaaca aactcctgga
     1141 catggcagcc caaattgcag aaggcatggc attcattgaa gagcggaatt atattcatcg
     1201 tgaccttcgg gctgccaaca ttctggtgtc tgacaccctg agctgcaaga ttgcagactt
     1261 tggcctagca cgcctcattg aggacaacga gtacacagcc agggaggggg ccaagtttcc
     1321 cattaagtgg acagcgccag aagccattaa ctacgggaca ttcaccatca agtcagatgt
     1381 gtggtctttt gggatcctgc tgacggaaat tgtcacccac ggccgcatcc cttacccagg
     1441 gatgaccaac ccggaggtga ttcagaacct ggagcgaggc taccgcatgg tgcgccctga
     1501 caactgtcca gaggagctgt accaactcat gaggctgtgc tggaaggagc gcccagagga
     1561 ccggcccacc tttgactacc tgcgcagtgt gctggaggac ttcttcacgg ccacagaggg
     1621 ccagtaccag cctcagcctt gagaggcctt gagaggccct ggggttctcc ccctttctct
     1681 ccagcctgac ttggggagat ggagttcttg tgccatagtc acatggccta tgcacatatg
     1741 gactctgcac atgaatccca cccacatgtg acacatatgc accttgtgtc tgtacacgtg
     1801 tcctgtagtt gcgtggactc tgcacatgtc ttgtacatgt gtagcctgtg catgtatgtc
     1861 ttggacactg tacaaggtac ccctttctgg ctctcccatt tcctgagacc acagagagag
     1921 gggagaagcc tgggattgac agaagcttct gcccacctac ttttctttcc tcagatcatc
     1981 cagaagttcc tcaagggcca ggactttatc taatacctct gtgtgctcct ccttggtgcc
     2041 tggcctggca cacatcagga gttcaataaa tgtctgttga tgactgttgt a
//
