LOCUS       NM_178834               2536 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens layilin (LAYN), transcript variant 2, mRNA.
ACCESSION   NM_178834
VERSION     NM_178834.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Arito,M., Tsutiya,A., Sato,M., Omoteyama,K., Sato,T., Motonaga,Y.,
            Suematsu,N., Kurokawa,M.S. and Kato,T.
  TITLE     Role of layilin in regulating mitochondria-mediated apoptosis: a
            study on B cell lymphoma (BCL)-2 family proteins
  JOURNAL   BMC Mol Cell Biol 25 (1), 24 (2024)
   PUBMED   39455917
  REMARK    GeneRIF: Role of layilin in regulating mitochondria-mediated
            apoptosis: a study on B cell lymphoma (BCL)-2 family proteins.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Liu,C., Wang,Y., Zhang,Y.H., Yuan,Z., Zhang,Z., Zeng,X., Guan,Z.,
            Bahabayi,A. and Lu,S.
  TITLE     Elevated Layilin-Positive Monocyte Levels in the Peripheral Blood
            of Patients with Systemic Lupus Erythematosus Reflect Their
            Autoimmune Status
  JOURNAL   Immunol Invest 52 (7), 879-896 (2023)
   PUBMED   37642473
  REMARK    GeneRIF: Elevated Layilin-Positive Monocyte Levels in the
            Peripheral Blood of Patients with Systemic Lupus Erythematosus
            Reflect Their Autoimmune Status.
REFERENCE   3  (bases 1 to 2536)
  AUTHORS   Jiawen,W., Jinfu,W., Jianyong,L., Yaoguang,Z. and Jianye,W.
  TITLE     Comprehensive landscape of the miRNA-regulated prognostic marker
            LAYN with immune infiltration and stemness in pan-cancer
  JOURNAL   J Cancer Res Clin Oncol 149 (13), 10989-11011 (2023)
   PUBMED   37335337
  REMARK    GeneRIF: Comprehensive landscape of the miRNA-regulated prognostic
            marker LAYN with immune infiltration and stemness in pan-cancer.
REFERENCE   4  (bases 1 to 2536)
  AUTHORS   Cao,L., Zhu,L. and Cheng,L.
  TITLE     ncRNA-Regulated LAYN Serves as a Prognostic Biomarker and
            Correlates with Immune Cell Infiltration in Hepatocellular
            Carcinoma: A Bioinformatics Analysis
  JOURNAL   Biomed Res Int 2022, 5357114 (2022)
   PUBMED   36398067
  REMARK    GeneRIF: ncRNA-Regulated LAYN Serves as a Prognostic Biomarker and
            Correlates with Immune Cell Infiltration in Hepatocellular
            Carcinoma: A Bioinformatics Analysis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2536)
  AUTHORS   Tsutiya,A., Arito,M., Tagashira,T., Sato,M., Omoteyama,K., Sato,T.,
            Suematsu,N., Kurokawa,M.S. and Kato,T.
  TITLE     Layilin promotes mitochondrial fission by cyclin-dependent kinase 1
            and dynamin-related protein 1 activation in HEK293T cells
  JOURNAL   Biochem Biophys Res Commun 549, 143-149 (2021)
   PUBMED   33676182
  REMARK    GeneRIF: Layilin promotes mitochondrial fission by cyclin-dependent
            kinase 1 and dynamin-related protein 1 activation in HEK293T cells.
REFERENCE   6  (bases 1 to 2536)
  AUTHORS   Forteza,R.M., Casalino-Matsuda,S.M., Falcon,N.S., Valencia
            Gattas,M. and Monzon,M.E.
  TITLE     Hyaluronan and layilin mediate loss of airway epithelial barrier
            function induced by cigarette smoke by decreasing E-cadherin
  JOURNAL   J Biol Chem 287 (50), 42288-42298 (2012)
   PUBMED   23048036
  REMARK    GeneRIF: Hyaluronan and layilin mediate loss of airway epithelial
            barrier function induced by cigarette smoke by decreasing
            E-cadherin.
REFERENCE   7  (bases 1 to 2536)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   8  (bases 1 to 2536)
  AUTHORS   Bono,P., Cordero,E., Johnson,K., Borowsky,M., Ramesh,V., Jacks,T.
            and Hynes,R.O.
  TITLE     Layilin, a cell surface hyaluronan receptor, interacts with merlin
            and radixin
  JOURNAL   Exp Cell Res 308 (1), 177-187 (2005)
   PUBMED   15913605
REFERENCE   9  (bases 1 to 2536)
  AUTHORS   Bono,P., Rubin,K., Higgins,J.M. and Hynes,R.O.
  TITLE     Layilin, a novel integral membrane protein, is a hyaluronan
            receptor
  JOURNAL   Mol Biol Cell 12 (4), 891-900 (2001)
   PUBMED   11294894
REFERENCE   10 (bases 1 to 2536)
  AUTHORS   Borowsky,M.L. and Hynes,R.O.
  TITLE     Layilin, a novel talin-binding transmembrane protein homologous
            with C-type lectins, is localized in membrane ruffles
  JOURNAL   J Cell Biol 143 (2), 429-442 (1998)
   PUBMED   9786953
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK055539.1, AK296231.1 and DA608521.1.
            
            On May 20, 2020 this sequence version replaced NM_178834.4.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' coding
            region, but maintains the reading frame, compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK055654.1, BC025407.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375614.7/ ENSP00000364764.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1854              AK055539.1         214-2067
            1855-2330           AK296231.1         1696-2171
            2331-2536           DA608521.1         342-547
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.1"
     gene            1..2536
                     /gene="LAYN"
                     /note="layilin"
                     /db_xref="GeneID:143903"
                     /db_xref="HGNC:HGNC:29471"
                     /db_xref="MIM:618843"
     exon            1..209
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1249
                     /gene="LAYN"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="layilin isoform 2 precursor"
                     /protein_id="NP_849156.1"
                     /db_xref="CCDS:CCDS31676.1"
                     /db_xref="GeneID:143903"
                     /db_xref="HGNC:HGNC:29471"
                     /db_xref="MIM:618843"
                     /translation="MRPGTALQAVLLAVLLVGLRAATGRLLSGQPVCRGGTQRPCYKV
                     IYFHDTSRRLNFEEAKEACRRDGGQLVSIESEDEQKLIEKFIENLLPSDGDFWIGLRR
                     REEKQSNSTACQDLYAWTDGSISQFRNWYVDEPSCGSEVCVVMYHQPSAPAGIGGPYM
                     FQWNDDRCNMKNNFICKYSDEKPAVPSREAEGEETELTTPVLPEETQEEDAKKTFKES
                     REAALNLAYILIPSIPLLLLLVVTTVVCWVWICRKRKREQPDPSTKKQHTIWPSPHQG
                     NSPDLEVYNVIRKQSEADLAETRPDLKNISFRVCSGEATPDDMSCDYDNMAVNPSESG
                     FVTLVSVESGFVTNDIYEFSPDQMGRSKESGWVENEIYGY"
     exon            210..507
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     exon            508..665
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     exon            666..698
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     exon            699..782
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     exon            783..885
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     exon            886..2536
                     /gene="LAYN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1726..1731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAYN"
                     /note="hexamer: AATAAA"
     regulatory      1736..1741
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAYN"
                     /note="hexamer: AATAAA"
     polyA_site      1751
                     /gene="LAYN"
                     /note="major polyA site"
     polyA_site      1761
                     /gene="LAYN"
     regulatory      2285..2290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAYN"
                     /note="hexamer: AATAAT"
     polyA_site      2303
                     /gene="LAYN"
ORIGIN      
        1 gtgcggtccg tcggtggcct agagatgctg ctgccgcggt tgcagttgtc gcgcacgcct
       61 ctgcccgcca gcccgctcca ccgccgtagc gcccgagtgt cggggggcgc acccgagtcg
      121 ggccatgagg ccgggaaccg cgctacaggc cgtgctgctg gccgtgctgc tggtggggct
      181 gcgggccgcg acgggtcgcc tgctgagtgg gcagccagtc tgccggggag ggacacagag
      241 gccttgttat aaagtcattt acttccatga tacttctcga agactgaact ttgaggaagc
      301 caaagaagcc tgcaggaggg atggaggcca gctagtcagc atcgagtctg aagatgaaca
      361 gaaactgata gaaaagttca ttgaaaacct cttgccatct gatggtgact tctggattgg
      421 gctcaggagg cgtgaggaga aacaaagcaa tagcacagcc tgccaggacc tttatgcttg
      481 gactgatggc agcatatcac aatttaggaa ctggtatgtg gatgagccat cctgcggcag
      541 cgaggtctgc gtggtcatgt accatcagcc atcggcaccc gctggcatcg gaggccccta
      601 catgttccag tggaatgatg accggtgcaa catgaagaac aatttcattt gcaaatattc
      661 tgatgagaaa ccagcagttc cttctagaga agctgaaggt gaggaaacag agctgacaac
      721 acctgtactt ccagaagaaa cacaggaaga agatgccaaa aaaacattta aagaaagtag
      781 agaagctgcc ttgaatctgg cctacatcct aatccccagc attccccttc tcctcctcct
      841 tgtggtcacc acagttgtat gttgggtttg gatctgtaga aaaagaaaac gggagcagcc
      901 agaccctagc acaaagaagc aacacaccat ctggccctct cctcaccagg gaaacagccc
      961 ggacctagag gtctacaatg tcataagaaa acaaagcgaa gctgacttag ctgagacccg
     1021 gccagacctg aagaatattt cattccgagt gtgttcggga gaagccactc ccgatgacat
     1081 gtcttgtgac tatgacaaca tggctgtgaa cccatcagaa agtgggtttg tgactctggt
     1141 gagcgtggag agtggatttg tgaccaatga catttatgag ttctccccag accaaatggg
     1201 gaggagtaag gagtctggat gggtggaaaa tgaaatatat ggttattagg acatataaaa
     1261 aactgaaact gacaacaatg gaaaagaaat gataagcaaa atcctcttat tttctataag
     1321 gaaaatacac agaaggtcta tgaacaagct tagatcaggt cctgtggatg agcatgtggt
     1381 ccccacgacc tcctgttgga cccccacgtt ttggctgtat cctttatccc agccagtcat
     1441 ccagctcgac cttatgagaa ggtaccttgc ccaggtctgg cacatagtag agtctcaata
     1501 aatgtcactt ggttggttgt atctaacttt taagggacag agctttacct ggcagtgata
     1561 aagatgggct gtggagcttg gaaaaccacc tctgttttcc ttgctctata cagcagcaca
     1621 tattatcata cagacagaaa atccagaatc ttttcaaagc ccacatatgg tagcacaggc
     1681 tggcctgtgc atcggcaatt ctcatatctg tttttttcaa agaataataa aatcaaataa
     1741 agagcaggaa acagagtgtt agtctgtgtc tacagccctt cctctgcatg tggccacagg
     1801 ggaccttttt ttgtttctcc tgacatccag acttggaaat atctaactac ttgcaaaact
     1861 aaaaatgagg ccaggcgcag tggctgacgc ctgtaatccc agaaccttgg gagaccaaga
     1921 ttggaggata gcttgagttc aggagttcca gaccttcctg ggcaaaatag tgagactctg
     1981 actctacaaa aaatttaaaa attagcaggg catggtggca tgcgcctgca gtcccagcta
     2041 ctcaggaggc cgaggtgggg gaattgcttg agcccaggag gttgaggctg cagtgagcta
     2101 ggatcacgcc actgctctcc tgcgtgggca acagaatgag accctgtctc taaacaaaaa
     2161 tattttttaa agaaactaaa aatgaaattt gagtctcagc ttctaggctt atgtactccc
     2221 atccctgata ctacacgttg ttctgtttgc atgtttgtta ctgcagcatc aacataacat
     2281 gactaataat gatgacagtt ctaccttttg ttgacagacc tcattgtttc aattgtgttt
     2341 ggcctcaaat tcagagaaaa gtttctcagc ccagcatcaa tcatatacac attggaggtc
     2401 tgaaagccat tatttaaatc accagcaatt tccctcaatc agcttcataa tctcatattt
     2461 taacctttcc tcatatgtct tattttttaa tagctcagca actttgcttc acatatggag
     2521 ccccaacctc tagagt
//
