LOCUS       NM_017773               3271 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens lymphocyte transmembrane adaptor 1 (LAX1), transcript
            variant 1, mRNA.
ACCESSION   NM_017773
VERSION     NM_017773.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3271)
  AUTHORS   Banton,M.C., Inder,K.L., Valk,E., Rudd,C.E. and Schneider,H.
  TITLE     Rab8 binding to immune cell-specific adaptor LAX facilitates
            formation of trans-Golgi network-proximal CTLA-4 vesicles for
            surface expression
  JOURNAL   Mol Cell Biol 34 (8), 1486-1499 (2014)
   PUBMED   24515439
  REMARK    GeneRIF: TRIM required LAX for binding to Rab8 in a complex, a
            novel CTLA-4/TRIM/LAX/Rab8 effector complex in the transport of
            CTLA-4 to the surfaces of T cells
REFERENCE   4  (bases 1 to 3271)
  AUTHORS   Hrdinka,M., Otahal,P. and Horejsi,V.
  TITLE     The transmembrane region is responsible for targeting of adaptor
            protein LAX into 'heavy rafts'
  JOURNAL   PLoS One 7 (5), e36330 (2012)
   PUBMED   22662118
  REMARK    GeneRIF: We identified the transmembrane domain of LAX as a first
            motif targeting transmembrane protein constructs to
            detergent-resistant heavy rafts, a novel type of membrane
            microdomains containing a number of physiologically important
            proteins.
REFERENCE   5  (bases 1 to 3271)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 3271)
  AUTHORS   Ehret,G.B., O'Connor,A.A., Weder,A., Cooper,R.S. and Chakravarti,A.
  TITLE     Follow-up of a major linkage peak on chromosome 1 reveals
            suggestive QTLs associated with essential hypertension: GenNet
            study
  JOURNAL   Eur J Hum Genet 17 (12), 1650-1657 (2009)
   PUBMED   19536175
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 3271)
  AUTHORS   Shapiro,M.J., Nguyen,C.T., Aghajanian,H., Zhang,W. and Shapiro,V.S.
  TITLE     Negative regulation of TCR signaling by linker for activation of X
            cells via phosphotyrosine-dependent and -independent mechanisms
  JOURNAL   J Immunol 181 (10), 7055-7061 (2008)
   PUBMED   18981125
  REMARK    GeneRIF: inhibition of signaling events involved in T cell
            activation by LAX occurs through mechanisms both dependent on and
            independent of its tyrosine phosphorylation.
REFERENCE   8  (bases 1 to 3271)
  AUTHORS   Zhu,M., Granillo,O., Wen,R., Yang,K., Dai,X., Wang,D. and Zhang,W.
  TITLE     Negative regulation of lymphocyte activation by the adaptor protein
            LAX
  JOURNAL   J Immunol 174 (9), 5612-5619 (2005)
   PUBMED   15843560
REFERENCE   9  (bases 1 to 3271)
  AUTHORS   Zhu,M., Janssen,E., Leung,K. and Zhang,W.
  TITLE     Molecular cloning of a novel gene encoding a membrane-associated
            adaptor protein (LAX) in lymphocyte signaling
  JOURNAL   J Biol Chem 277 (48), 46151-46158 (2002)
   PUBMED   12359715
  REMARK    GeneRIF: functions to negatively regulate signaling in lymphocytes
            GeneRIF: Overexpression of LAX in Jurkat cells specifically
            inhibits T cell receptor-mediated p38 MAPK activation and NFAT/AP-1
            transcriptional activation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC414389.1, BC089408.1 and AC114402.2.
            
            On May 17, 2019 this sequence version replaced NM_017773.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089408.1, SRR1163655.64134.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000442561.7/ ENSP00000406970.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                DC414389.1         28-53
            27-2298             BC089408.1         1-2272
            2299-2477           BC089408.1         2274-2452
            2478-2478           AC114402.2         84650-84650
            2479-3118           BC089408.1         2454-3093
            3119-3119           AC114402.2         85291-85291
            3120-3271           BC089408.1         3095-3246
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..3271
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /note="lymphocyte transmembrane adaptor 1"
                     /db_xref="GeneID:54900"
                     /db_xref="HGNC:HGNC:26005"
                     /db_xref="MIM:619622"
     exon            1..472
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /inference="alignment:Splign:2.1.0"
     CDS             384..1580
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /note="isoform a is encoded by transcript variant 1;
                     LAT-like membrane associated protein; linker for
                     activation of x cells; lymphocyte transmembrane adapter 1;
                     membrane-associated adapter protein LAX"
                     /codon_start=1
                     /product="lymphocyte transmembrane adapter 1 isoform a"
                     /protein_id="NP_060243.2"
                     /db_xref="CCDS:CCDS1441.2"
                     /db_xref="GeneID:54900"
                     /db_xref="HGNC:HGNC:26005"
                     /db_xref="MIM:619622"
                     /translation="MDGVTPTLSTIRGRTLESSTLHVTPRSLDRNKDQITNIFSGFAG
                     LLAILLVVAVFCILWNWNKRKKRQVPYLRVTVMPLLTLPQTRQRAKNIYDILPWRQED
                     LGRHESRSMRIFSTESLLSRNSESPEHVPSQAGNAFQEHTAHIHATEYAVGIYDNAMV
                     PQMCGNLTPSAHCINVRASRDCASISSEDSHDYVNVPTAEEIAETLASTKSPSRNLFV
                     LPSTQKLEFTEERDEGCGDAGDCTSLYSPGAEDSDSLSNGEGSSQISNDYVNMTGLDL
                     SAIQERQLWVAFQCCRDYENVPAADPSGSQQQAEKDVPSSNIGHVEDKTDDPGTHVQC
                     VKRTFLASGDYADFQPFTQSEDSQMKHREEMSNEDSSDYENVLTAKLGGRDSEQGPGT
                     QLLPDE"
     exon            473..582
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /inference="alignment:Splign:2.1.0"
     exon            583..693
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /inference="alignment:Splign:2.1.0"
     exon            694..773
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /inference="alignment:Splign:2.1.0"
     exon            774..3271
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3138
                     /gene="LAX1"
                     /gene_synonym="LAX"
                     /note="major polyA site"
ORIGIN      
        1 actctgcttt gggtgttgaa ggaagacgag cttctcacgg agcctctctt gagcctcttg
       61 gcagtttccc cctctgtgcc cctcacgttt ccaccagaaa cgtgaaggca gaggccacag
      121 attctccctg agccacctca cttggaagca ccatgtccgg atgagatcgc acttcctgca
      181 gtgggcatta gccacgtcca ggtagaacca aacctgttgc ttttgtatgt tgggtcaact
      241 tggcctgacg tttcagaggt agacacgaga tagggagttt gttgcggggg tggggagaag
      301 tggtagacat gctggctaac tgattatgat taagagaaac ttagaagctg aagccagaga
      361 gcatctcaaa ggttcctgat acaatggatg gtgtcactcc aaccctttcg acaatcagag
      421 ggaggacctt ggagtccagc actctgcatg tgactccccg cagcctggac agaaataaag
      481 accagatcac caacatcttt tccgggtttg cgggactcct cgccatcctc ctggtcgttg
      541 cggttttctg catcttgtgg aattggaata aacggaagaa gcgacaagtt ccttacctcc
      601 gagttaccgt catgcccttg ctgactttgc cacaaaccag acaaagagcc aaaaatattt
      661 atgacatctt gccttggcga caggaagacc tggggagaca tgagtcgagg agtatgcgca
      721 ttttcagtac tgagagcctc ctctccagaa attctgagag cccggagcat gtgccctccc
      781 aagcaggcaa tgccttccag gagcatacag cccacatcca tgccacagag tacgcggtgg
      841 gtatctatga caacgccatg gtcccccaga tgtgtgggaa cctcactccc tcggcacact
      901 gcatcaatgt cagagcttcc agagactgcg caagcatttc ttcagaggat tcgcatgatt
      961 atgtcaatgt ccccacagca gaagagattg ctgagactct agcttctacc aaaagccctt
     1021 ccagaaatct ctttgttctt cccagtaccc agaagctgga gtttactgag gaaagagatg
     1081 agggctgtgg agatgctggt gactgcacca gtttgtattc tccaggagct gaggacagtg
     1141 attcactcag caatggagaa ggttcttctc agatctcaaa tgactatgtc aacatgacag
     1201 ggttggatct cagtgccatc caggaaaggc agctctgggt ggcttttcag tgctgcagag
     1261 actatgaaaa tgttccagca gcagatccca gtggaagcca gcagcaggct gagaaagatg
     1321 tgccatcctc aaacataggt catgtcgagg acaagacaga tgatcccggg acccatgtcc
     1381 aatgtgtcaa aaggacattc cttgcttcag gggattatgc agactttcag ccattcacac
     1441 agagtgagga cagtcagatg aaacatagag aagagatgtc aaatgaggac tccagtgact
     1501 atgaaaatgt gctaactgcc aagttaggag gcagggactc tgagcagggg cctggcactc
     1561 agctccttcc tgatgaatga agacccaggt acccagccat aaagccacat tgagtagtct
     1621 atcccatagg attgactact gcagagtcta gtgcagaccc gtgatcacct tagtgcttca
     1681 gtggattcac tggttagatt aaaaagaggc tgagatgagc agtgaactaa gaggccacac
     1741 aaaagcagag gtttgggaat tccagaaggg aattcttctc aagcagagtg tggttatctc
     1801 ctgtaccagc ctaagaatgt ttgctgaaac tgcttcctag aactgtgaag aaagcaggaa
     1861 agtagtgcac agtagtctaa gattattacc ttcattaata ccaacaggct gcaaagcaag
     1921 agtatagatt attgtataat ccagtcagag gtcaaaagga aggaagaagt tggagtggag
     1981 tggggtgggc aatttccatt ttaaagagtg taggcaggcc aggtgcagtg gctcatgcct
     2041 gtaatcccag cattttggta ggccgaggag ggcagatcac ttggtgccag gagttctaga
     2101 ccagcctggc caacatgacc catctttact aaaaatacaa aaattagcca ggcgtggtgg
     2161 tgtgcccctg taatcccagc tactcaggag gctgaagcag gagaatcgct tgaacccggg
     2221 aggtggaggt tgcagcaagc cgagattgtg ccactgtact ccagcctggt gacagagcga
     2281 gactccctct caaaaaaaaa aacaaacaaa aaagaagtgt gggcaaccaa atgtaggtaa
     2341 ggatgaagaa gaacaggaac tctcactggt ggctgatggg attgcaaagc gtcagggcag
     2401 ctttgaaaaa cttttcagca gtttcttata agcataactt atcatatggc ccagccattt
     2461 cactactaga aattgacgca agtgaactga aaacttatgt tcacacaaaa actcgcacat
     2521 gactgtttat agcagcttca ttcataatta ccaaaaacta gaaataaccc agatatcctt
     2581 caacaggtga ttggataagc tgttgatatc cattccttga gtttatccat actcaacagt
     2641 aaaaaatgaa ctcttggttc acacaataat atagacatat cttaaatcca ttttgctaag
     2701 tgaaagaagc taggcctaaa agtctacatg ttgggcgatt ctatttatat gacattctgg
     2761 aaaaggcaaa actataggga tgaagaacag atcagttgtt gtcaggtttg agaaaaggag
     2821 ggaagtttgc tacaaagggg ctgcaaaggg aataggctga tggagctgtt ctgtatggta
     2881 ctgtgtggtg gatacatgac aatatccatt tgcaaacaca agaactatac acatggccag
     2941 gcgcggtggc tcacacccgt aatcccagca ctttgcgggg ccgaggcagg ggaacacaag
     3001 gtcaggagat caagactgtc ctggacaaca tggtgaaacc ctgtctctac tgtctctaac
     3061 atttgtaaac ctgtctctaa tttgtaattt gtaaaccctg tctctcatat ttgtaaaact
     3121 acaaaaatta gcttggcatg gtggtgtgcg cctgtagtcc cagctactag gaggctgagg
     3181 caggagaatc acttgaacca ggaaggcgga ctgcattcca gcctgggcaa cagagaaaga
     3241 ctctgtctca aaaaaaaaaa aaaaaaaaaa a
//
