LOCUS       NM_031206               2470 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens LAS1 like ribosome biogenesis factor (LAS1L),
            transcript variant 1, mRNA.
ACCESSION   NM_031206
VERSION     NM_031206.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Li,Y., Yang,Y., Sears,R.C., Dai,M.S. and Sun,X.X.
  TITLE     USP36 SUMOylates Las1L and Promotes Its Function in Pre-Ribosomal
            RNA ITS2 Processing
  JOURNAL   Cancer Res Commun 4 (10), 2835-2845 (2024)
   PUBMED   39356143
  REMARK    GeneRIF: USP36 SUMOylates Las1L and Promotes Its Function in
            Pre-Ribosomal RNA ITS2 Processing.
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Weeks,S.E., Kammerud,S.C., Metge,B.J., AlSheikh,H.A.,
            Schneider,D.A., Chen,D., Wei,S., Mobley,J.A., Ojesina,A.I.,
            Shevde,L.A. and Samant,R.S.
  TITLE     Inhibiting beta-catenin disables nucleolar functions in
            triple-negative breast cancer
  JOURNAL   Cell Death Dis 12 (3), 242 (2021)
   PUBMED   33664239
  REMARK    GeneRIF: Inhibiting beta-catenin disables nucleolar functions in
            triple-negative breast cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2470)
  AUTHORS   Gordon,J., Pillon,M.C. and Stanley,R.E.
  TITLE     Nol9 Is a Spatial Regulator for the Human ITS2 Pre-rRNA
            Endonuclease-Kinase Complex
  JOURNAL   J Mol Biol 431 (19), 3771-3786 (2019)
   PUBMED   31288032
REFERENCE   4  (bases 1 to 2470)
  AUTHORS   Fanis,P., Gillemans,N., Aghajanirefah,A., Pourfarzad,F.,
            Demmers,J., Esteghamat,F., Vadlamudi,R.K., Grosveld,F.,
            Philipsen,S. and van Dijk,T.B.
  TITLE     Five friends of methylated chromatin target of
            protein-arginine-methyltransferase[prmt]-1 (chtop), a complex
            linking arginine methylation to desumoylation
  JOURNAL   Mol Cell Proteomics 11 (11), 1263-1273 (2012)
   PUBMED   22872859
  REMARK    GeneRIF: Recruitment of the protein complex 5FMC to Zbp-89, a
            zinc-finger transcription factor, affects its sumoylation status
            and transactivation potential.[5FMC]
REFERENCE   5  (bases 1 to 2470)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 2470)
  AUTHORS   Schillewaert,S., Wacheul,L., Lhomme,F. and Lafontaine,D.L.
  TITLE     The evolutionarily conserved protein Las1 is required for pre-rRNA
            processing at both ends of ITS2
  JOURNAL   Mol Cell Biol 32 (2), 430-444 (2012)
   PUBMED   22083961
  REMARK    GeneRIF: Analysis of high-molecular-weight RNAs confirmed that
            Las1L is required for ITS2 processing,which separates the 5.8S and
            25S/28S rRNAs, as 32S was found to accumulate and 12S to diminish
            upon Las1L depletion.
REFERENCE   7  (bases 1 to 2470)
  AUTHORS   Pfefferle,S., Schopf,J., Kogl,M., Friedel,C.C., Muller,M.A.,
            Carbajo-Lozoya,J., Stellberger,T., von Dall'Armi,E., Herzog,P.,
            Kallies,S., Niemeyer,D., Ditt,V., Kuri,T., Zust,R., Pumpor,K.,
            Hilgenfeld,R., Schwarz,F., Zimmer,R., Steffen,I., Weber,F.,
            Thiel,V., Herrler,G., Thiel,H.J., Schwegmann-Wessels,C.,
            Pohlmann,S., Haas,J., Drosten,C. and von Brunn,A.
  TITLE     The SARS-coronavirus-host interactome: identification of
            cyclophilins as target for pan-coronavirus inhibitors
  JOURNAL   PLoS Pathog 7 (10), e1002331 (2011)
   PUBMED   22046132
REFERENCE   8  (bases 1 to 2470)
  AUTHORS   Castle,C.D., Cassimere,E.K., Lee,J. and Denicourt,C.
  TITLE     Las1L is a nucleolar protein required for cell proliferation and
            ribosome biogenesis
  JOURNAL   Mol Cell Biol 30 (18), 4404-4414 (2010)
   PUBMED   20647540
  REMARK    GeneRIF: Data demonstrate that Las1L is essential for cell
            proliferation and biogenesis of the 60S ribosomal subunit.
REFERENCE   9  (bases 1 to 2470)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   10 (bases 1 to 2470)
  AUTHORS   Scherl,A., Coute,Y., Deon,C., Calle,A., Kindbeiter,K.,
            Sanchez,J.C., Greco,A., Hochstrasser,D. and Diaz,J.J.
  TITLE     Functional proteomic analysis of human nucleolus
  JOURNAL   Mol Biol Cell 13 (11), 4100-4109 (2002)
   PUBMED   12429849
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA619280.1 and AF116730.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jan 3, 2020 this sequence version replaced NM_031206.6.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF116730.1, BC014545.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374811.8/ ENSP00000363944.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                DA619280.1         20-62
            44-2470             AF116730.1         1-2427
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..2470
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /note="LAS1 like ribosome biogenesis factor"
                     /db_xref="GeneID:81887"
                     /db_xref="HGNC:HGNC:25726"
                     /db_xref="MIM:300964"
     exon            1..308
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2277
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein LAS1 homolog; ribosomal biogenesis protein LAS1L;
                     endoribonuclease LAS1L"
                     /codon_start=1
                     /product="ribosomal biogenesis protein LAS1L isoform 1"
                     /protein_id="NP_112483.1"
                     /db_xref="CCDS:CCDS14381.1"
                     /db_xref="GeneID:81887"
                     /db_xref="HGNC:HGNC:25726"
                     /db_xref="MIM:300964"
                     /translation="MSWESGAGPGLGSQGMDLVWSAWYGKCVKGKGSLPLSAHGIVVA
                     WLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLDV
                     TGGLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCLAQEVNIPDWIVDLRHELTH
                     KKMPHINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEFREGIEEEDQEEDKNIV
                     VDDITEQKPEPQDDGKSTESDVKADGDSKGSEEVDSHCKKALSHKELYERARELLVSY
                     EEEQFTVLEKFRYLPKAIKAWNNPSPRVECVLAELKGVTCENREAVLDAFLDDGFLVP
                     TFEQLAALQIEYEDGQTEVQRGEGTDPKSHKNVDLNDVLVPKPFSQFWQPLLRGLHSQ
                     NFTQALLERMLSELPALGISGIRPTYILRWTVELIVANTKTGRNARRFSAGQWEARRG
                     WRLFNCSASLDWPRMVESCLGSPCWASPQLLRIIFKAMGQGLPDEEQEKLLRICSIYT
                     QSGENSLVQEGSEASPIGKSPYTLDSLYWSVKPASSSFGSEAKAQQQEEQGSVNDVKE
                     EEKEEKEVLPDQVEEEEENDDQEEEEEDEDDEDDEEEDRMEVGPFSTGQESPTAENAR
                     LLAQKRGALQGSAWQVSSEDVRWDTFPLGRMPGQTEDPAELMLENYDTMYLLDQPVLE
                     QRLEPSTCKTDTLGLSCGVGSGNCSNSSSSNFEGLLWSQGQLHGLKTGLQLF"
     exon            309..434
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            435..504
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            505..586
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            587..871
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            872..918
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1028
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1114
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1165
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1372
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1520
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1999
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2150
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2470
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2453..2458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /note="hexamer: AATAAA"
     polyA_site      2470
                     /gene="LAS1L"
                     /gene_synonym="dJ475B7.2; Las1; Las1-like; MRXSWTS; WTS"
                     /note="major polyA site"
ORIGIN      
        1 ggagtgaggg tagcacgctg agctgaaggc tgtgcggagc ggcgcggcac agagcctgtt
       61 gttgagctca gtatgtcgtg ggaatccggg gccgggccag gtctaggttc ccaggggatg
      121 gatctcgtgt ggagtgcgtg gtacggaaag tgcgttaaag ggaaagggtc gttgccactc
      181 tcggcccacg gcatcgtggt cgcctggctc agcagggccg agtgggacca ggtgacggtt
      241 tatctgttct gtgacgacca taagttgcag cggtacgcgc ttaaccgcat cacggtgtgg
      301 aggagcaggt caggcaacga actccctctg gcagtggctt ctactgctga cctgatacgc
      361 tgtaagctct tggatgtaac tggtggcttg ggcactgatg aacttagact gctctatggc
      421 atggcattgg tcaggtttgt gaatcttatc tcagagagga agacaaagtt tgccaaggtc
      481 cccctcaagt gtctggctca agaggtaaat attccggatt ggattgttga ccttcgccat
      541 gagttgaccc acaagaaaat gccccatata aatgactgcc gcagaggctg ctactttgtc
      601 ctggattggc tccagaagac ctattggtgc cgccaactgg agaacagcct gagagagacc
      661 tgggagttgg aggagttcag ggaagggata gaggaagagg atcaagagga agataagaac
      721 attgttgttg atgacatcac agaacagaaa ccagagcctc aggatgatgg gaaaagtacg
      781 gagtcagatg taaaggccga tggagacagc aaaggcagcg aagaggtgga ttctcattgc
      841 aaaaaggccc tgagtcataa agagctatat gaaagagccc gagaactgct ggtatcatac
      901 gaagaggagc agtttacggt gctggagaaa tttaggtatt tacctaaggc cattaaggcg
      961 tggaataacc cgtccccacg tgtagaatgt gtcctggcag agctcaaggg cgttacatgc
     1021 gagaacaggg aggctgtgct ggatgctttt ctggatgatg gcttccttgt ccccacattt
     1081 gaacagttgg cagctttgca gatagaatat gaagatggtc agactgaggt ccagagaggg
     1141 gaaggtactg acccaaagtc acacaaaaac gtggacttga atgacgtcct ggtgccaaag
     1201 ccgttctctc agttctggca gcccctgctc aggggcctgc actcccagaa cttcacgcag
     1261 gccctattgg agaggatgct ctctgaactg ccagccttgg ggatcagcgg gatccggcct
     1321 acctacatcc tcagatggac cgttgaactg atcgtggcca acaccaagac tggacggaat
     1381 gctcgccgat tttctgcagg ccagtgggaa gcaagaaggg gctggaggct gttcaactgc
     1441 tccgcctccc ttgactggcc ccggatggtt gagtcctgct tgggctcacc ttgctgggcc
     1501 agcccccaac tccttcggat catcttcaaa gccatggggc agggcctgcc agacgaggag
     1561 caggagaagc tgctgcgcat ctgttccatt tatacccaga gtggagaaaa cagcctggtg
     1621 caggagggct ctgaggcctc ccccattggg aagtctccat atacactaga cagcctgtat
     1681 tggagcgtca agccagccag ctccagcttc gggtctgaag caaaggccca gcaacaggag
     1741 gagcagggca gtgttaatga tgtcaaggaa gaggagaagg aggagaaaga ggtcttgcca
     1801 gaccaggtag aggaggagga agaaaatgat gaccaagagg aggaagagga ggatgaagat
     1861 gatgaagatg atgaagagga agacagaatg gaggtggggc ctttctctac agggcaagag
     1921 tcccccactg ccgagaatgc taggcttctg gcccagaaaa gaggagcttt gcagggctct
     1981 gcatggcagg ttagctcaga agacgtgcga tgggacacat ttcccctagg ccgaatgcca
     2041 ggtcagaccg aggacccagc agagctcatg ctggagaatt atgacaccat gtatcttttg
     2101 gaccagcctg tgctagagca gcggctggaa ccctcaacat gcaagactga caccttgggc
     2161 ctgagctgtg gtgtcggcag tggcaactgc agcaacagca gcagcagcaa cttcgagggc
     2221 cttctctgga gccaggggca gctgcatggg ctcaaaactg gcctgcagct cttctgatgg
     2281 ccatccctgg tgcaagtgtt catccagccg tgccagggca acagcccacc ccctagtaca
     2341 actgatgctc cctgagacaa cctgggagac agcctggatc agccacatca actcagttgt
     2401 ccaccacagg ggaattttga atgtcttttg tttttgtttt gttttgaaaa ataataaaca
     2461 ggcaactgta
//
