LOCUS       NM_020117               4760 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant
            1, mRNA.
ACCESSION   NM_020117
VERSION     NM_020117.11
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Chen,W., Lin,Y., Jiang,M., Wang,Q. and Shu,Q.
  TITLE     Identification of LARS as an essential gene for osteosarcoma
            proliferation through large-Scale CRISPR-Cas9 screening database
            and experimental verification
  JOURNAL   J Transl Med 20 (1), 355 (2022)
   PUBMED   35962451
  REMARK    GeneRIF: Identification of LARS as an essential gene for
            osteosarcoma proliferation through large-Scale CRISPR-Cas9
            screening database and experimental verification.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Kim,K., Yoo,H.C., Kim,B.G., Kim,S., Sung,Y., Yoon,I., Yu,Y.C.,
            Park,S.J., Kim,J.H., Myung,K., Hwang,K.Y., Kim,S. and Han,J.M.
  TITLE     O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates
            leucine and glucose availability to regulate mTORC1 and the
            metabolic fate of leucine
  JOURNAL   Nat Commun 13 (1), 2904 (2022)
   PUBMED   35614056
  REMARK    GeneRIF: O-GlcNAc modification of leucyl-tRNA synthetase 1
            integrates leucine and glucose availability to regulate mTORC1 and
            the metabolic fate of leucine.
            Erratum:[Nat Commun. 2022 Oct 21;13(1):6253. doi:
            10.1038/s41467-022-34146-3.. PMID: 36271081]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4760)
  AUTHORS   Bae,J.H. and Kim,J.H.
  TITLE     Leucyl-tRNA synthetase 1 is required for proliferation of TSC-null
            cells
  JOURNAL   Biochem Biophys Res Commun 571, 159-166 (2021)
   PUBMED   34325132
  REMARK    GeneRIF: Leucyl-tRNA synthetase 1 is required for proliferation of
            TSC-null cells.
REFERENCE   4  (bases 1 to 4760)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4760)
  AUTHORS   Tabolacci,E., Molinario,C., Marangi,G., Nobile,V., Arena,V.,
            Mendes,M.I., Smith,D.E.C., Salomons,G.S., Tana,M., Costa,S.,
            Vento,G. and Genuardi,M.
  TITLE     Infantile Liver Failure Syndrome 1 associated with a novel variant
            of the LARS1 gene: Clinical, genetic, and functional
            characterization
  JOURNAL   Clin Genet 99 (4), 601-603 (2021)
   PUBMED   33314043
  REMARK    GeneRIF: Infantile Liver Failure Syndrome 1 associated with a novel
            variant of the LARS1 gene: Clinical, genetic, and functional
            characterization.
REFERENCE   6  (bases 1 to 4760)
  AUTHORS   Lamour,V., Quevillon,S., Diriong,S., N'Guyen,V.C., Lipinski,M. and
            Mirande,M.
  TITLE     Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme
            as a case of horizontal gene transfer
  JOURNAL   Proc Natl Acad Sci U S A 91 (18), 8670-8674 (1994)
   PUBMED   8078941
REFERENCE   7  (bases 1 to 4760)
  AUTHORS   Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
            Noda,T.
  TITLE     Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
            of the anticodon-binding domain and acquisition of a new structural
            unit
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7435-7439 (1994)
   PUBMED   8052601
REFERENCE   8  (bases 1 to 4760)
  AUTHORS   Norcum,M.T.
  TITLE     Structural analysis of the high molecular mass aminoacyl-tRNA
            synthetase complex. Effects of neutral salts and detergents
  JOURNAL   J Biol Chem 266 (23), 15398-15405 (1991)
   PUBMED   1651330
REFERENCE   9  (bases 1 to 4760)
  AUTHORS   Dana,S. and Wasmuth,J.J.
  TITLE     Selective linkage disruption in human-Chinese hamster cell hybrids:
            deletion mapping of the leuS, hexB, emtB, and chr genes on human
            chromosome 5
  JOURNAL   Mol Cell Biol 2 (10), 1220-1228 (1982)
   PUBMED   7177110
REFERENCE   10 (bases 1 to 4760)
  AUTHORS   Giles,R.E., Shimizu,N. and Ruddle,F.H.
  TITLE     Assignment of a human genetic locus to chromosome 5 which corrects
            the heat sensitive lesion associated with reduced leucyl-tRNA
            synthetase activity in ts025Cl Chinese hamster cells
  JOURNAL   Somatic Cell Genet 6 (5), 667-687 (1980)
   PUBMED   6933703
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB037773.1, AC091887.3 and
            CK902545.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020117.10.
            
            Summary: This gene encodes a cytosolic leucine-tRNA synthetase, a
            member of the class I aminoacyl-tRNA synthetase family. The encoded
            enzyme catalyzes the ATP-dependent ligation of L-leucine to
            tRNA(Leu). It is found in the cytoplasm as part of a
            multisynthetase complex and interacts with the arginine tRNA
            synthetase through its C-terminal domain. A mutation in this gene
            was found in affected individuals with infantile liver failure
            syndrome 1. Alternatively spliced transcript variants of this gene
            have been observed. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.12971.1,
                                           SRR1803614.84918.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394434.7/ ENSP00000377954.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3883              AB037773.1         11-3893
            3884-4697           AC091887.3         97324-98137         c
            4698-4760           CK902545.1         28-90               c
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..4760
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="leucyl-tRNA synthetase 1"
                     /db_xref="GeneID:51520"
                     /db_xref="HGNC:HGNC:6512"
                     /db_xref="MIM:151350"
     exon            1..163
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..3688
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /EC_number="6.1.1.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine tRNA ligase 1, cytoplasmic; proliferation-inducing
                     gene 44; cytoplasmic leucyl-tRNA synthetase; cytosolic
                     leucyl-tRNA synthetase; leucine translase"
                     /codon_start=1
                     /product="leucine--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_064502.9"
                     /db_xref="CCDS:CCDS34265.1"
                     /db_xref="GeneID:51520"
                     /db_xref="HGNC:HGNC:6512"
                     /db_xref="MIM:151350"
                     /translation="MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKG
                     KYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACAD
                     KLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGI
                     MKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFV
                     RWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYP
                     SKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNM
                     SYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSV
                     PSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELK
                     IQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYM
                     EPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLG
                     WLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLG
                     IRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHL
                     SYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMR
                     LALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFA
                     SELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQT
                     LLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLK
                     NYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVI
                     ASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNS
                     LELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIR
                     QGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAV
                     FNVDLMSKKIHLTENGIRVDIGDTIIYLVH"
     exon            164..282
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            283..370
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            371..451
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            452..589
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            590..751
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            752..864
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            865..928
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            929..996
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1222
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1310
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1387
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1441
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1582
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1660
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1746
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1812
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1895
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2034
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2247
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2305
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2369
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2553
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2554..2644
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2785
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2926
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3037
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3148
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3253
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3254..3349
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3482
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..4760
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3684..3689
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: ATTAAA"
     polyA_site      3704
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
     regulatory      3863..3868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: AATAAA"
     polyA_site      3883
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="major polyA site"
     regulatory      4733..4738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: AATAAA"
     polyA_site      4760
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
ORIGIN      
        1 agtaaggagc cggatgattg cctcagcagg tgtgaagcgt gtgctttagt ttcgtgggag
       61 gcctggcatc cccgagaggg aggggaaagg taaccactcc tttgtggagg tcgccagggt
      121 cattgtcgtg gatttgcaca gtcggctggg cggtgcaatg gcggaaagaa aaggaacagc
      181 caaagtggac tttttgaaga agattgagaa agaaatccaa cagaaatggg atactgagag
      241 agtgtttgag gtcaatgcat ctaatttaga gaaacagacc agcaagggca agtattttgt
      301 aaccttccca tatccatata tgaatggacg ccttcatttg ggacacacgt tttctttatc
      361 caaatgtgag tttgctgtag ggtaccagcg attgaaagga aaatgttgtc tgtttccctt
      421 tggcctgcac tgtactggaa tgcctattaa ggcatgtgct gataagttga aaagagaaat
      481 agagctgtat ggttgccccc ctgattttcc agatgaagaa gaggaagagg aagaaaccag
      541 tgttaaaaca gaagatataa taattaagga taaagctaaa ggaaaaaaga gtaaagctgc
      601 tgctaaagct ggatcttcta aataccagtg gggcattatg aaatcccttg gcctgtctga
      661 tgaagagata gtaaaatttt ctgaagcaga acattggctt gattatttcc cgccactggc
      721 tattcaggat ttaaaaagaa tgggtttgaa ggtagactgg cgtcgttcct tcatcaccac
      781 tgatgttaat ccttactatg attcatttgt cagatggcaa tttttaacat taagagaaag
      841 aaacaaaatt aaatttggga agcggtatac aatttactct ccgaaagatg gacagccttg
      901 catggatcat gatagacaaa ctggagaggg tgttggacct caggaatata ctttactcaa
      961 attgaaggtg cttgagccat acccatctaa attaagtggc ctgaaaggta aaaatatttt
     1021 cttggtggct gctactctca gacctgagac catgtttggg cagacaaatt gttgggttcg
     1081 tcctgatatg aagtacattg gatttgagac ggtgaatggt gatatattca tctgtaccca
     1141 aaaagcagcc aggaatatgt cataccaggg ctttaccaaa gacaatggcg tggtgcctgt
     1201 tgttaaggaa ttaatggggg aggaaattct tggtgcatca ctttctgcac ctttaacatc
     1261 atacaaggtg atctatgttc tcccaatgct aactattaag gaggataaag gcactggtgt
     1321 ggttacaagt gttccttccg actcccctga tgatattgct gccctcagag acttgaagaa
     1381 aaagcaagcc ttacgagcaa aatatggaat tagagatgac atggtcttgc catttgagcc
     1441 ggtgccagtc attgaaatcc caggttttgg aaatctttct gctgtaacca tttgtgatga
     1501 gttgaaaatt cagagccaga atgaccggga aaaacttgca gaagcaaagg agaagatata
     1561 tctaaaagga ttttatgagg gtatcatgtt ggtggatgga tttaaaggac agaaggttca
     1621 agatgtaaag aagactattc agaaaaagat gattgacgct ggagatgcac ttatttacat
     1681 ggaaccagag aaacaagtga tgtccaggtc gtcagatgaa tgtgttgtgg ctctgtgtga
     1741 ccagtggtac ttggattatg gagaagagaa ttggaagaaa cagacatctc agtgcttgaa
     1801 gaacctggaa acattctgtg aggagaccag gaggaatttt gaagccacct taggttggct
     1861 acaagaacat gcttgctcaa gaacttatgg tctaggcact cacctgcctt gggatgagca
     1921 gtggctgatt gaatcacttt ctgactccac tatttacatg gcattttaca cagttgcaca
     1981 cctattgcag gggggtaact tgcatggaca ggcagagtct ccgctgggca ttagaccgca
     2041 acagatgacc aaggaagttt gggattatgt tttcttcaag gaggctccat ttcctaagac
     2101 tcagattgca aaggaaaaat tagatcagtt aaagcaggag tttgaattct ggtatcctgt
     2161 tgatcttcgc gtctctggca aggatcttgt tccaaatcat ctttcatatt acctttataa
     2221 tcatgtggct atgtggccgg aacaaagtga caaatggcct acagctgtga gagcaaatgg
     2281 acatctcctc ctgaactctg agaagatgtc aaaatccaca ggcaacttcc tcactttgac
     2341 ccaagctatt gacaaatttt cagcagatgg aatgcgtttg gctctggctg atgctggtga
     2401 cactgtagaa gatgccaact ttgtggaagc catggcagat gcaggtattc tccgtctgta
     2461 cacctgggta gagtgggtga aagaaatggt tgccaactgg gacagcctaa gaagtggtcc
     2521 tgccagcact ttcaatgata gagtttttgc cagtgaattg aatgcaggaa ttataaaaac
     2581 agatcaaaac tatgaaaaga tgatgtttaa agaagctttg aaaacagggt tttttgagtt
     2641 tcaggccgca aaagataagt accgtgaatt ggctgtggaa gggatgcaca gagaacttgt
     2701 gttccggttt attgaagttc agacacttct cctcgctcca ttctgtccac atttgtgtga
     2761 gcacatctgg acactcctgg gaaagcctga ctcaattatg aatgcttcat ggcctgtggc
     2821 aggtcctgtt aatgaagttt taatacactc ctcacagtat cttatggaag taacacatga
     2881 ccttagacta cgactcaaga actatatgat gccagctaaa gggaagaaga ctgacaaaca
     2941 acccctgcag aagccctcac attgcaccat ctatgtggca aagaactatc caccttggca
     3001 acataccacc ctgtctgttc tacgtaaaca ctttgaggcc aataacggaa aactgcctga
     3061 caacaaagtc attgctagtg aactaggcag tatgccagaa ctgaagaaat acatgaagaa
     3121 agtcatgcca tttgttgcca tgattaagga aaatctggag aagatggggc ctcgtattct
     3181 ggatttgcaa ttagaatttg atgaaaaggc tgtgcttatg gagaatatag tctatctgac
     3241 taattcgctt gagctagaac acatagaagt caagtttgcc tccgaagcag aagataaaat
     3301 cagggaagac tgctgtcctg ggaaaccact taatgttttt agaatagaac ctggtgtgtc
     3361 cgtttctctg gtgaatcccc agccatccaa tggccacttc tcaaccaaaa ttgaaatcag
     3421 gcaaggagat aactgtgatt ccataatcag gcgtttaatg aaaatgaatc gaggaattaa
     3481 agacctttcc aaagtgaaac tgatgagatt tgatgatcca ctgttggggc ctcgacgagt
     3541 tcctgtcctg ggaaaggagt acaccgagaa gacccccatt tctgagcatg ctgttttcaa
     3601 tgtggacctc atgagcaaga aaattcatct gactgagaat gggataaggg tggatattgg
     3661 cgatacaata atctatctgg ttcattaaac tcatgcacat tggagattta tcctggtttc
     3721 ttaggaatac tactactctg attgtgtcta ctgattggct atcagaacct taggctggac
     3781 ctaaatagat tgatttcatt tctaaccatc caattctgca tgtattcata attctatcaa
     3841 gtcatctttg attcctggac ctaataaatt ttttttccct ttctttgggt gtccaagaga
     3901 aatggttttt gccaaactct ttttaaaaaa caaattgttg ctatttccta gaagtttctg
     3961 gtttttaaga tgaacataaa agtgtcagta tgcttctttt atgaggtgta ctttatactt
     4021 tgatgaaggc taaggtgtac ctaacagctt tttatagtat attcatttat ggagttagct
     4081 gtattttttt ttaaaaaaga aaagtagtaa attagcagct ttttttttta aatgaagaga
     4141 acttcatgat gatttaaaag aactaatgag aatattttga cttgacttca attactaagt
     4201 attttgtata aagaacaaat aggtcctgca ttttataaga ctttttaaag ctttaattat
     4261 tcaactaaga accaataatg gcttcttcaa ggctaatatc aatagtttat aagcagtata
     4321 tttcacctac actaaatatg ggcattttaa aagaagattc cccccttccc ccccagaaat
     4381 ctgcatttac taaatttgtc ttaagtccaa ttgtgtttaa aggtcatgct ggtcgggtgt
     4441 ggtggctcac acctataatc ccagcacttt gggaggccaa ggtgggcagt cacctgaggt
     4501 caggagtttg agaccagcct gaccaacatg gtgaaacccc gtctctacta aaaatacaaa
     4561 aattagcttg gcatggtggc gggtacctgt aatcccagct gcttgggagt gtgaggcagg
     4621 agaatcgctt gaacctggga gaccgaggtt gcagtgagct gaaaccgtgc cattgcactc
     4681 cagcttaggc aacaagagtg aaactctgtc tcaatcaatc catcaatcaa tcaataaaat
     4741 agaggtcatg ctgactatta
//
