LOCUS       NM_001317964            4622 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens leucyl-tRNA synthetase 1 (LARS1), transcript variant
            3, mRNA.
ACCESSION   NM_001317964 XM_011537655
VERSION     NM_001317964.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Chen,W., Lin,Y., Jiang,M., Wang,Q. and Shu,Q.
  TITLE     Identification of LARS as an essential gene for osteosarcoma
            proliferation through large-Scale CRISPR-Cas9 screening database
            and experimental verification
  JOURNAL   J Transl Med 20 (1), 355 (2022)
   PUBMED   35962451
  REMARK    GeneRIF: Identification of LARS as an essential gene for
            osteosarcoma proliferation through large-Scale CRISPR-Cas9
            screening database and experimental verification.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Kim,K., Yoo,H.C., Kim,B.G., Kim,S., Sung,Y., Yoon,I., Yu,Y.C.,
            Park,S.J., Kim,J.H., Myung,K., Hwang,K.Y., Kim,S. and Han,J.M.
  TITLE     O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates
            leucine and glucose availability to regulate mTORC1 and the
            metabolic fate of leucine
  JOURNAL   Nat Commun 13 (1), 2904 (2022)
   PUBMED   35614056
  REMARK    GeneRIF: O-GlcNAc modification of leucyl-tRNA synthetase 1
            integrates leucine and glucose availability to regulate mTORC1 and
            the metabolic fate of leucine.
            Erratum:[Nat Commun. 2022 Oct 21;13(1):6253. doi:
            10.1038/s41467-022-34146-3.. PMID: 36271081]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4622)
  AUTHORS   Bae,J.H. and Kim,J.H.
  TITLE     Leucyl-tRNA synthetase 1 is required for proliferation of TSC-null
            cells
  JOURNAL   Biochem Biophys Res Commun 571, 159-166 (2021)
   PUBMED   34325132
  REMARK    GeneRIF: Leucyl-tRNA synthetase 1 is required for proliferation of
            TSC-null cells.
REFERENCE   4  (bases 1 to 4622)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4622)
  AUTHORS   Tabolacci,E., Molinario,C., Marangi,G., Nobile,V., Arena,V.,
            Mendes,M.I., Smith,D.E.C., Salomons,G.S., Tana,M., Costa,S.,
            Vento,G. and Genuardi,M.
  TITLE     Infantile Liver Failure Syndrome 1 associated with a novel variant
            of the LARS1 gene: Clinical, genetic, and functional
            characterization
  JOURNAL   Clin Genet 99 (4), 601-603 (2021)
   PUBMED   33314043
  REMARK    GeneRIF: Infantile Liver Failure Syndrome 1 associated with a novel
            variant of the LARS1 gene: Clinical, genetic, and functional
            characterization.
REFERENCE   6  (bases 1 to 4622)
  AUTHORS   Lamour,V., Quevillon,S., Diriong,S., N'Guyen,V.C., Lipinski,M. and
            Mirande,M.
  TITLE     Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme
            as a case of horizontal gene transfer
  JOURNAL   Proc Natl Acad Sci U S A 91 (18), 8670-8674 (1994)
   PUBMED   8078941
REFERENCE   7  (bases 1 to 4622)
  AUTHORS   Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
            Noda,T.
  TITLE     Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
            of the anticodon-binding domain and acquisition of a new structural
            unit
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7435-7439 (1994)
   PUBMED   8052601
REFERENCE   8  (bases 1 to 4622)
  AUTHORS   Norcum,M.T.
  TITLE     Structural analysis of the high molecular mass aminoacyl-tRNA
            synthetase complex. Effects of neutral salts and detergents
  JOURNAL   J Biol Chem 266 (23), 15398-15405 (1991)
   PUBMED   1651330
REFERENCE   9  (bases 1 to 4622)
  AUTHORS   Dana,S. and Wasmuth,J.J.
  TITLE     Selective linkage disruption in human-Chinese hamster cell hybrids:
            deletion mapping of the leuS, hexB, emtB, and chr genes on human
            chromosome 5
  JOURNAL   Mol Cell Biol 2 (10), 1220-1228 (1982)
   PUBMED   7177110
REFERENCE   10 (bases 1 to 4622)
  AUTHORS   Giles,R.E., Shimizu,N. and Ruddle,F.H.
  TITLE     Assignment of a human genetic locus to chromosome 5 which corrects
            the heat sensitive lesion associated with reduced leucyl-tRNA
            synthetase activity in ts025Cl Chinese hamster cells
  JOURNAL   Somatic Cell Genet 6 (5), 667-687 (1980)
   PUBMED   6933703
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB091795.1, AB037773.1,
            AK304134.1, AC091887.3 and CK902545.1.
            
            On Aug 14, 2020 this sequence version replaced NM_001317964.1.
            
            Summary: This gene encodes a cytosolic leucine-tRNA synthetase, a
            member of the class I aminoacyl-tRNA synthetase family. The encoded
            enzyme catalyzes the ATP-dependent ligation of L-leucine to
            tRNA(Leu). It is found in the cytoplasm as part of a
            multisynthetase complex and interacts with the arginine tRNA
            synthetase through its C-terminal domain. A mutation in this gene
            was found in affected individuals with infantile liver failure
            syndrome 1. Alternatively spliced transcript variants of this gene
            have been observed. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (3) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. The encoded
            isoform (3) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK304134.1, SRR11853563.27241.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                DB091795.1         138-165
            29-72               AB037773.1         39-82
            73-513              AK304134.1         26-466
            514-3745            AB037773.1         662-3893
            3746-4559           AC091887.3         97324-98137         c
            4560-4622           CK902545.1         28-90               c
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..4622
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="leucyl-tRNA synthetase 1"
                     /db_xref="GeneID:51520"
                     /db_xref="HGNC:HGNC:6512"
                     /db_xref="MIM:151350"
     exon            1..163
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..3550
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /EC_number="6.1.1.4"
                     /note="isoform 3 is encoded by transcript variant 3;
                     leucine tRNA ligase 1, cytoplasmic; proliferation-inducing
                     gene 44; cytoplasmic leucyl-tRNA synthetase; cytosolic
                     leucyl-tRNA synthetase; leucine translase"
                     /codon_start=1
                     /product="leucine--tRNA ligase, cytoplasmic isoform 3"
                     /protein_id="NP_001304893.1"
                     /db_xref="CCDS:CCDS93798.1"
                     /db_xref="GeneID:51520"
                     /db_xref="HGNC:HGNC:6512"
                     /db_xref="MIM:151350"
                     /translation="MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKG
                     KYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKSKAA
                     AKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFI
                     TTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEY
                     TLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGD
                     IFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTI
                     KEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFG
                     NLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQK
                     KMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFC
                     EETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQG
                     GNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDL
                     RVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLT
                     QAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRS
                     GPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMH
                     RELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYL
                     MEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFE
                     ANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKA
                     VLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQP
                     SNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKE
                     YTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH"
     exon            164..282
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            283..370
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            371..451
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            452..613
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            614..726
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            727..790
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            791..858
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1084
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1172
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1249
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1303
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1444
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1522
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1608
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1674
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1757
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1896
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2109
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2167
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2231
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2415
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2506
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2647
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2788
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2899
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            2900..3010
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..3115
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3116..3211
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3344
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..4622
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3546..3551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: ATTAAA"
     polyA_site      3566
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
     regulatory      3725..3730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: AATAAA"
     polyA_site      3745
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="major polyA site"
     regulatory      4595..4600
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
                     /note="hexamer: AATAAA"
     polyA_site      4622
                     /gene="LARS1"
                     /gene_synonym="cLRS; hr025Cl; HSPC192; ILFS1; LARS; LEURS;
                     LEUS; LFIS; LRS; PIG44; RNTLS"
ORIGIN      
        1 agtaaggagc cggatgattg cctcagcagg tgtgaagcgt gtgctttagt ttcgtgggag
       61 gcctggcatc cccgagaggg aggggaaagg taaccactcc tttgtggagg tcgccagggt
      121 cattgtcgtg gatttgcaca gtcggctggg cggtgcaatg gcggaaagaa aaggaacagc
      181 caaagtggac tttttgaaga agattgagaa agaaatccaa cagaaatggg atactgagag
      241 agtgtttgag gtcaatgcat ctaatttaga gaaacagacc agcaagggca agtattttgt
      301 aaccttccca tatccatata tgaatggacg ccttcatttg ggacacacgt tttctttatc
      361 caaatgtgag tttgctgtag ggtaccagcg attgaaagga aaatgttgtc tgtttccctt
      421 tggcctgcac tgtactggaa tgcctattaa gagtaaagct gctgctaaag ctggatcttc
      481 taaataccag tggggcatta tgaaatccct tggcctgtct gatgaagaga tagtaaaatt
      541 ttctgaagca gaacattggc ttgattattt cccgccactg gctattcagg atttaaaaag
      601 aatgggtttg aaggtagact ggcgtcgttc cttcatcacc actgatgtta atccttacta
      661 tgattcattt gtcagatggc aatttttaac attaagagaa agaaacaaaa ttaaatttgg
      721 gaagcggtat acaatttact ctccgaaaga tggacagcct tgcatggatc atgatagaca
      781 aactggagag ggtgttggac ctcaggaata tactttactc aaattgaagg tgcttgagcc
      841 atacccatct aaattaagtg gcctgaaagg taaaaatatt ttcttggtgg ctgctactct
      901 cagacctgag accatgtttg ggcagacaaa ttgttgggtt cgtcctgata tgaagtacat
      961 tggatttgag acggtgaatg gtgatatatt catctgtacc caaaaagcag ccaggaatat
     1021 gtcataccag ggctttacca aagacaatgg cgtggtgcct gttgttaagg aattaatggg
     1081 ggaggaaatt cttggtgcat cactttctgc acctttaaca tcatacaagg tgatctatgt
     1141 tctcccaatg ctaactatta aggaggataa aggcactggt gtggttacaa gtgttccttc
     1201 cgactcccct gatgatattg ctgccctcag agacttgaag aaaaagcaag ccttacgagc
     1261 aaaatatgga attagagatg acatggtctt gccatttgag ccggtgccag tcattgaaat
     1321 cccaggtttt ggaaatcttt ctgctgtaac catttgtgat gagttgaaaa ttcagagcca
     1381 gaatgaccgg gaaaaacttg cagaagcaaa ggagaagata tatctaaaag gattttatga
     1441 gggtatcatg ttggtggatg gatttaaagg acagaaggtt caagatgtaa agaagactat
     1501 tcagaaaaag atgattgacg ctggagatgc acttatttac atggaaccag agaaacaagt
     1561 gatgtccagg tcgtcagatg aatgtgttgt ggctctgtgt gaccagtggt acttggatta
     1621 tggagaagag aattggaaga aacagacatc tcagtgcttg aagaacctgg aaacattctg
     1681 tgaggagacc aggaggaatt ttgaagccac cttaggttgg ctacaagaac atgcttgctc
     1741 aagaacttat ggtctaggca ctcacctgcc ttgggatgag cagtggctga ttgaatcact
     1801 ttctgactcc actatttaca tggcatttta cacagttgca cacctattgc aggggggtaa
     1861 cttgcatgga caggcagagt ctccgctggg cattagaccg caacagatga ccaaggaagt
     1921 ttgggattat gttttcttca aggaggctcc atttcctaag actcagattg caaaggaaaa
     1981 attagatcag ttaaagcagg agtttgaatt ctggtatcct gttgatcttc gcgtctctgg
     2041 caaggatctt gttccaaatc atctttcata ttacctttat aatcatgtgg ctatgtggcc
     2101 ggaacaaagt gacaaatggc ctacagctgt gagagcaaat ggacatctcc tcctgaactc
     2161 tgagaagatg tcaaaatcca caggcaactt cctcactttg acccaagcta ttgacaaatt
     2221 ttcagcagat ggaatgcgtt tggctctggc tgatgctggt gacactgtag aagatgccaa
     2281 ctttgtggaa gccatggcag atgcaggtat tctccgtctg tacacctggg tagagtgggt
     2341 gaaagaaatg gttgccaact gggacagcct aagaagtggt cctgccagca ctttcaatga
     2401 tagagttttt gccagtgaat tgaatgcagg aattataaaa acagatcaaa actatgaaaa
     2461 gatgatgttt aaagaagctt tgaaaacagg gttttttgag tttcaggccg caaaagataa
     2521 gtaccgtgaa ttggctgtgg aagggatgca cagagaactt gtgttccggt ttattgaagt
     2581 tcagacactt ctcctcgctc cattctgtcc acatttgtgt gagcacatct ggacactcct
     2641 gggaaagcct gactcaatta tgaatgcttc atggcctgtg gcaggtcctg ttaatgaagt
     2701 tttaatacac tcctcacagt atcttatgga agtaacacat gaccttagac tacgactcaa
     2761 gaactatatg atgccagcta aagggaagaa gactgacaaa caacccctgc agaagccctc
     2821 acattgcacc atctatgtgg caaagaacta tccaccttgg caacatacca ccctgtctgt
     2881 tctacgtaaa cactttgagg ccaataacgg aaaactgcct gacaacaaag tcattgctag
     2941 tgaactaggc agtatgccag aactgaagaa atacatgaag aaagtcatgc catttgttgc
     3001 catgattaag gaaaatctgg agaagatggg gcctcgtatt ctggatttgc aattagaatt
     3061 tgatgaaaag gctgtgctta tggagaatat agtctatctg actaattcgc ttgagctaga
     3121 acacatagaa gtcaagtttg cctccgaagc agaagataaa atcagggaag actgctgtcc
     3181 tgggaaacca cttaatgttt ttagaataga acctggtgtg tccgtttctc tggtgaatcc
     3241 ccagccatcc aatggccact tctcaaccaa aattgaaatc aggcaaggag ataactgtga
     3301 ttccataatc aggcgtttaa tgaaaatgaa tcgaggaatt aaagaccttt ccaaagtgaa
     3361 actgatgaga tttgatgatc cactgttggg gcctcgacga gttcctgtcc tgggaaagga
     3421 gtacaccgag aagaccccca tttctgagca tgctgttttc aatgtggacc tcatgagcaa
     3481 gaaaattcat ctgactgaga atgggataag ggtggatatt ggcgatacaa taatctatct
     3541 ggttcattaa actcatgcac attggagatt tatcctggtt tcttaggaat actactactc
     3601 tgattgtgtc tactgattgg ctatcagaac cttaggctgg acctaaatag attgatttca
     3661 tttctaacca tccaattctg catgtattca taattctatc aagtcatctt tgattcctgg
     3721 acctaataaa ttttttttcc ctttctttgg gtgtccaaga gaaatggttt ttgccaaact
     3781 ctttttaaaa aacaaattgt tgctatttcc tagaagtttc tggtttttaa gatgaacata
     3841 aaagtgtcag tatgcttctt ttatgaggtg tactttatac tttgatgaag gctaaggtgt
     3901 acctaacagc tttttatagt atattcattt atggagttag ctgtattttt ttttaaaaaa
     3961 gaaaagtagt aaattagcag cttttttttt taaatgaaga gaacttcatg atgatttaaa
     4021 agaactaatg agaatatttt gacttgactt caattactaa gtattttgta taaagaacaa
     4081 ataggtcctg cattttataa gactttttaa agctttaatt attcaactaa gaaccaataa
     4141 tggcttcttc aaggctaata tcaatagttt ataagcagta tatttcacct acactaaata
     4201 tgggcatttt aaaagaagat tccccccttc ccccccagaa atctgcattt actaaatttg
     4261 tcttaagtcc aattgtgttt aaaggtcatg ctggtcgggt gtggtggctc acacctataa
     4321 tcccagcact ttgggaggcc aaggtgggca gtcacctgag gtcaggagtt tgagaccagc
     4381 ctgaccaaca tggtgaaacc ccgtctctac taaaaataca aaaattagct tggcatggtg
     4441 gcgggtacct gtaatcccag ctgcttggga gtgtgaggca ggagaatcgc ttgaacctgg
     4501 gagaccgagg ttgcagtgag ctgaaaccgt gccattgcac tccagcttag gcaacaagag
     4561 tgaaactctg tctcaatcaa tccatcaatc aatcaataaa atagaggtca tgctgactat
     4621 ta
//
