LOCUS       NM_014017                621 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens late endosomal/lysosomal adaptor, MAPK and MTOR
            activator 2 (LAMTOR2), transcript variant 1, mRNA.
ACCESSION   NM_014017
VERSION     NM_014017.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Xu,J., Quan,G., Huang,W. and Jiang,J.
  TITLE     VSIG2 promotes malignant progression of pancreatic ductal
            adenocarcinoma by enhancing LAMTOR2-mediated mTOR activation
  JOURNAL   Cell Commun Signal 21 (1), 223 (2023)
   PUBMED   37626304
  REMARK    GeneRIF: VSIG2 promotes malignant progression of pancreatic ductal
            adenocarcinoma by enhancing LAMTOR2-mediated mTOR activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 621)
  AUTHORS   Jansen,R.M., Peruzzo,R., Fromm,S.A., Yokom,A.L., Zoncu,R. and
            Hurley,J.H.
  TITLE     Structural basis for FLCN RagC GAP activation in MiT-TFE
            substrate-selective mTORC1 regulation
  JOURNAL   Sci Adv 8 (37), eadd2926 (2022)
   PUBMED   36103527
REFERENCE   4  (bases 1 to 621)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 621)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 621)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 621)
  AUTHORS   Huang,L., Pan,C.Q., Li,B., Tucker-Kellogg,L., Tidor,B., Chen,Y. and
            Low,B.C.
  TITLE     Simulating EGFR-ERK signaling control by scaffold proteins KSR and
            MP1 reveals differential ligand-sensitivity co-regulated by
            Cbl-CIN85 and endophilin
  JOURNAL   PLoS One 6 (8), e22933 (2011)
   PUBMED   21829671
  REMARK    GeneRIF: simulations constitute a multi-dimensional exploration of
            how EGF-dependent EGFR endocytosis and ERK activation are
            dynamically affected by scaffolds KSR and MP1, co-regulated by
            Cbl-CIN85 and Endophilin A1
REFERENCE   8  (bases 1 to 621)
  AUTHORS   Sancak,Y., Bar-Peled,L., Zoncu,R., Markhard,A.L., Nada,S. and
            Sabatini,D.M.
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and
            is necessary for its activation by amino acids
  JOURNAL   Cell 141 (2), 290-303 (2010)
   PUBMED   20381137
  REMARK    GeneRIF: A complex encoded by the MAPKSP1, ROBLD3, and c11orf59
            genes interacts with the Rag GTPases, recruits them to lysosomes,
            and is essential for mTORC1 activation
REFERENCE   9  (bases 1 to 621)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   10 (bases 1 to 621)
  AUTHORS   Wunderlich,W., Fialka,I., Teis,D., Alpi,A., Pfeifer,A.,
            Parton,R.G., Lottspeich,F. and Huber,L.A.
  TITLE     A novel 14-kilodalton protein interacts with the mitogen-activated
            protein kinase scaffold mp1 on a late endosomal/lysosomal
            compartment
  JOURNAL   J Cell Biol 152 (4), 765-776 (2001)
   PUBMED   11266467
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL355388.30, BC024190.2 and
            CA438137.1.
            
            On Aug 31, 2019 this sequence version replaced NM_014017.3.
            
            Summary: The product of this gene is highly conserved with a mouse
            protein associated with the cytoplasmic face of late endosomes and
            lysosomes. The mouse protein interacts with MAPK scaffold protein
            1, a component of the mitogen-activated protein kinase pathway. In
            humans, a mutation in this gene has been associated with a primary
            immunodeficiency syndrome, and suggests a role for this protein in
            endosomal biogenesis. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Feb 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CN360668.1, BG708390.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368305.9/ ENSP00000357288.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                AL355388.30        148462-148529
            69-619              BC024190.2         1-551
            620-621             CA438137.1         23-24               c
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..621
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /note="late endosomal/lysosomal adaptor, MAPK and MTOR
                     activator 2"
                     /db_xref="GeneID:28956"
                     /db_xref="HGNC:HGNC:29796"
                     /db_xref="MIM:610389"
     exon            1..176
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..486
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     roadblock domain containing 3; MAPKSP1 adaptor protein;
                     ragulator complex protein LAMTOR2; mitogen-activated
                     protein-binding protein-interacting protein (MAPBPIP);
                     endosomal adaptor protein p14; roadblock domain-containing
                     protein 3; late endosomal/lysosomal Mp1-interacting
                     protein; late endosomal/lysosomal adaptor and MAPK and
                     MTOR activator 2; MAPBP-interacting protein"
                     /codon_start=1
                     /product="ragulator complex protein LAMTOR2 isoform 1"
                     /protein_id="NP_054736.1"
                     /db_xref="CCDS:CCDS1128.1"
                     /db_xref="GeneID:28956"
                     /db_xref="HGNC:HGNC:29796"
                     /db_xref="MIM:610389"
                     /translation="MLRPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARV
                     TAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFG
                     MLKAKAQALVQYLEEPLTQVAAS"
     exon            177..339
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /inference="alignment:Splign:2.1.0"
     exon            340..429
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /inference="alignment:Splign:2.1.0"
     exon            430..621
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      601..606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /note="hexamer: AATAAA"
     polyA_site      621
                     /gene="LAMTOR2"
                     /gene_synonym="ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14;
                     Ragulator2; ROBLD3"
                     /note="major polyA site"
ORIGIN      
        1 agggcgtccc ggagcaggcc aacgggacta cgggaagcag cgggcagcgg cccgcgggag
       61 gcacctcgga gatctgggtg caaaagccca gggttaggaa ccgtaggcat gctgcgcccc
      121 aaggctttga cccaggtgct aagccaagcc aacactggag gcgtccagag caccctgctg
      181 ctgaataacg agggatcact gctggcctac tctggttacg gggacactga cgcccgggtc
      241 accgctgcca tagccagtaa catctgggcc gcctacgacc ggaacgggaa ccaagcgttt
      301 aatgaagaca atctcaaatt catcctcatg gactgcatgg agggccgtgt agccatcacc
      361 cgagtggcca accttctgct gtgtatgtat gccaaggaga ccgtgggctt tggaatgctc
      421 aaggccaagg cccaggcttt ggtgcagtac ctggaggagc ccctcaccca agtggcggca
      481 tcttaacggc attggtggaa gctggggtca gaaaagagaa atgaccattt ggaggggcgg
      541 ggcctcctag aagaaccttc ttagacaatg gggggaggat gggactttgt tttttccaag
      601 aataaacttc aactcctgtc a
//
