LOCUS       NM_005562               5398 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens laminin subunit gamma 2 (LAMC2), transcript variant 1,
            mRNA.
ACCESSION   NM_005562
VERSION     NM_005562.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Zhang,J., Ji,F., Tan,Y., Zhao,L., Zhao,Y., Liu,J., Shao,L., Shi,J.,
            Ye,M., He,X., Jin,J., Zhao,B., Huang,J., Roessler,S., Zheng,X. and
            Ji,J.
  TITLE     Oncogenic Roles of Laminin Subunit Gamma-2 in Intrahepatic
            Cholangiocarcinoma via Promoting EGFR Translation
  JOURNAL   Adv Sci (Weinh) 11 (21), e2309010 (2024)
   PUBMED   38526177
  REMARK    GeneRIF: Oncogenic Roles of Laminin Subunit Gamma-2 in Intrahepatic
            Cholangiocarcinoma via Promoting EGFR Translation.
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Wang,D., Keyoumu,K., Yu,R., Wen,D., Jiang,H., Liu,X., Di,X. and
            Zhang,S.
  TITLE     Extracellular matrix marker LAMC2 targets ZEB1 to promote TNBC
            malignancy via up-regulating CD44/STAT3 signaling pathway
  JOURNAL   Mol Med 30 (1), 61 (2024)
   PUBMED   38760717
  REMARK    GeneRIF: Extracellular matrix marker LAMC2 targets ZEB1 to promote
            TNBC malignancy via up-regulating CD44/STAT3 signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5398)
  AUTHORS   Cheng,L., Li,X., Dong,W., Yang,J., Li,P., Qiang,X., Yin,J. and
            Guo,L.
  TITLE     LAMC2 regulates the proliferation, invasion, and metastasis of
            gastric cancer via PI3K/Akt signaling pathway
  JOURNAL   J Cancer Res Clin Oncol 150 (5), 230 (2024)
   PUBMED   38703300
  REMARK    GeneRIF: LAMC2 regulates the proliferation, invasion, and
            metastasis of gastric cancer via PI3K/Akt signaling pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5398)
  AUTHORS   Liu,J., Wang,M., Wang,M., Wang,F. and Zhang,B.
  TITLE     LncRNAs-Regulated High Expression of LAMC2 Reveals a Prognostic and
            Immunological Value in Pancreatic Adenocarcinoma
  JOURNAL   Biochem Genet 62 (1), 485-503 (2024)
   PUBMED   37382751
  REMARK    GeneRIF: LncRNAs-Regulated High Expression of LAMC2 Reveals a
            Prognostic and Immunological Value in Pancreatic Adenocarcinoma.
REFERENCE   5  (bases 1 to 5398)
  AUTHORS   Tong,D., Wang,X., Liu,L., Wen,T., Chen,Q. and Huang,C.
  TITLE     LAMC2 promotes EGFR cell membrane localization and acts as a novel
            biomarker for tyrosine kinase inhibitors (TKIs) sensitivity in lung
            cancer
  JOURNAL   Cancer Gene Ther 30 (11), 1498-1512 (2023)
   PUBMED   37542131
  REMARK    GeneRIF: LAMC2 promotes EGFR cell membrane localization and acts as
            a novel biomarker for tyrosine kinase inhibitors (TKIs) sensitivity
            in lung cancer.
REFERENCE   6  (bases 1 to 5398)
  AUTHORS   Baudoin,C., Miquel,C., Gagnoux-Palacios,L., Pulkkinen,L.,
            Christiano,A.M., Uitto,J., Tadini,G., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A novel homozygous nonsense mutation in the LAMC2 gene in patients
            with the Herlitz junctional epidermolysis bullosa
  JOURNAL   Hum Mol Genet 3 (10), 1909-1910 (1994)
   PUBMED   7849725
REFERENCE   7  (bases 1 to 5398)
  AUTHORS   Gedde-Dahl,T. Jr., Dupuy,B.M., Jonassen,R., Winberg,J.O.,
            Anton-Lamprecht,I. and Olaisen,B.
  TITLE     Junctional epidermolysis bullosa inversa (locus EBR2A) assigned to
            1q31 by linkage and association to LAMC1
  JOURNAL   Hum Mol Genet 3 (8), 1387-1391 (1994)
   PUBMED   7987320
REFERENCE   8  (bases 1 to 5398)
  AUTHORS   Burgeson,R.E., Chiquet,M., Deutzmann,R., Ekblom,P., Engel,J.,
            Kleinman,H., Martin,G.R., Meneguzzi,G., Paulsson,M., Sanes,J. et
            al.
  TITLE     A new nomenclature for the laminins
  JOURNAL   Matrix Biol 14 (3), 209-211 (1994)
   PUBMED   7921537
REFERENCE   9  (bases 1 to 5398)
  AUTHORS   Pfendner,E.G. and Lucky,A.W.
  TITLE     Junctional Epidermolysis Bullosa
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301304
REFERENCE   10 (bases 1 to 5398)
  AUTHORS   Kallunki,P., Sainio,K., Eddy,R., Byers,M., Kallunki,T., Sariola,H.,
            Beck,K., Hirvonen,H., Shows,T.B. and Tryggvason,K.
  TITLE     A truncated laminin chain homologous to the B2 chain: structure,
            spatial expression, and chromosomal assignment
  JOURNAL   J Cell Biol 119 (3), 679-693 (1992)
   PUBMED   1383240
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL354953.16 and X73902.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_005562.2.
            
            Summary: Laminins, a family of extracellular matrix glycoproteins,
            are the major noncollagenous constituent of basement membranes.
            They have been implicated in a wide variety of biological processes
            including cell adhesion, differentiation, migration, signaling,
            neurite outgrowth and metastasis. Laminins, composed of 3 non
            identical chains: laminin alpha, beta and gamma (formerly A, B1,
            and B2, respectively), have a cruciform structure consisting of 3
            short arms, each formed by a different chain, and a long arm
            composed of all 3 chains. Each laminin chain is a multidomain
            protein encoded by a distinct gene. Several isoforms of each chain
            have been described. Different alpha, beta and gamma chain isomers
            combine to give rise to different heterotrimeric laminin isoforms
            which are designated by Arabic numerals in the order of their
            discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The
            biological functions of the different chains and trimer molecules
            are largely unknown, but some of the chains have been shown to
            differ with respect to their tissue distribution, presumably
            reflecting diverse functions in vivo. This gene encodes the gamma
            chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought
            to be a truncated version of beta chain (B2t), is highly homologous
            to the gamma 1 chain; however, it lacks domain VI, and domains V,
            IV and III are shorter. It is expressed in several fetal tissues
            but differently from gamma 1, and is specifically localized to
            epithelial cells in skin, lung and kidney. The gamma 2 chain
            together with alpha 3 and beta 3 chains constitute laminin 5
            (earlier known as kalinin), which is an integral part of the
            anchoring filaments that connect epithelial cells to the underlying
            basement membrane. The epithelium-specific expression of the gamma
            2 chain implied its role as an epithelium attachment molecule, and
            mutations in this gene have been associated with junctional
            epidermolysis bullosa, a skin disease characterized by blisters due
            to disruption of the epidermal-dermal junction. Two transcript
            variants resulting from alternative splicing of the 3' terminal
            exon, and encoding different isoforms of gamma 2 chain, have been
            described. The two variants are differentially expressed in
            embryonic tissues, however, the biological significance of the two
            forms is not known. Transcript variants utilizing alternative
            polyA_signal have also been noted in literature. [provided by
            RefSeq, Aug 2011].
            
            Transcript Variant:  This variant (1) represents the full length
            transcript variant. It encodes isoform (a) which is expressed in
            the epithelia of embryonic tissues.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because transcript sequence consistent with
            the reference genome assembly was not available for all regions of
            the RefSeq transcript. The extent of this transcript is supported
            by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X73902.1, Z15008.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264144.5/ ENSP00000264144.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AL354953.16        92286-92410
            126-4526            X73902.1           102-4502
            4527-5398           AL354953.16        150278-151149
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.3"
     gene            1..5398
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /note="laminin subunit gamma 2"
                     /db_xref="GeneID:3918"
                     /db_xref="HGNC:HGNC:6493"
                     /db_xref="MIM:150292"
     exon            1..168
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..3671
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; BM600-100kDa; laminin B2t chain; CSF 140 kDa
                     subunit; nicein subunit gamma; kalinin subunit gamma;
                     ladsin 140 kDa subunit; epiligrin subunit gamma;
                     cell-scattering factor 140 kDa subunit; large adhesive
                     scatter factor 140 kDa subunit; laminin, gamma 2"
                     /codon_start=1
                     /product="laminin subunit gamma-2 isoform a precursor"
                     /protein_id="NP_005553.2"
                     /db_xref="CCDS:CCDS1352.1"
                     /db_xref="GeneID:3918"
                     /db_xref="HGNC:HGNC:6493"
                     /db_xref="MIM:150292"
                     /translation="MPALWLGCCLCFSLLLPAARATSRREVCDCNGKSRQCIFDRELH
                     RQTGNGFRCLNCNDNTDGIHCEKCKNGFYRHRERDRCLPCNCNSKGSLSARCDNSGRC
                     SCKPGVTGARCDRCLPGFHMLTDAGCTQDQRLLDSKCDCDPAGIAGPCDAGRCVCKPA
                     VTGERCDRCRSGYYNLDGGNPEGCTQCFCYGHSASCRSSAEYSVHKITSTFHQDVDGW
                     KAVQRNGSPAKLQWSQRHQDVFSSAQRLDPVYFVAPAKFLGNQQVSYGQSLSFDYRVD
                     RGGRHPSAHDVILEGAGLRITAPLMPLGKTLPCGLTKTYTFRLNEHPSNNWSPQLSYF
                     EYRRLLRNLTALRIRATYGEYSTGYIDNVTLISARPVSGAPAPWVEQCICPVGYKGQF
                     CQDCASGYKRDSARLGPFGTCIPCNCQGGGACDPDTGDCYSGDENPDIECADCPIGFY
                     NDPHDPRSCKPCPCHNGFSCSVMPETEEVVCNNCPPGVTGARCELCADGYFGDPFGEH
                     GPVRPCQPCQCNNNVDPSASGNCDRLTGRCLKCIHNTAGIYCDQCKAGYFGDPLAPNP
                     ADKCRACNCNPMGSEPVGCRSDGTCVCKPGFGGPNCEHGAFSCPACYNQVKIQMDQFM
                     QQLQRMEALISKAQGGDGVVPDTELEGRMQQAEQALQDILRDAQISEGASRSLGLQLA
                     KVRSQENSYQSRLDDLKMTVERVRALGSQYQNRVRDTHRLITQMQLSLAESEASLGNT
                     NIPASDHYVGPNGFKSLAQEATRLAESHVESASNMEQLTRETEDYSKQALSLVRKALH
                     EGVGSGSGSPDGAVVQGLVEKLEKTKSLAQQLTREATQAEIEADRSYQHSLRLLDSVS
                     RLQGVSDQSFQVEEAKRIKQKADSLSSLVTRHMDEFKRTQKNLGNWKEEAQQLLQNGK
                     SGREKSDQLLSRANLAKSRAQEALSMGNATFYEVESILKNLREFDLQVDNRKAEAEEA
                     MKRLSYISQKVSDASDKTQQAERALGSAAADAQRAKNGAGEALEISSEIEQEIGSLNL
                     EANVTADGALAMEKGLASLKSEMREVEGELERKELEFDTNMDAVQMVITEAQKVDTRA
                     KNAGVTIQDTLNTLDGLLHLMDQPLSVDEEGLVLLEQKLSRAKTQINSQLRPMMSELE
                     ERARQQRGHLHLLETSIDGILADVKNLENIRDNLPPGCYNTQALEQQ"
     exon            169..357
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            358..493
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            494..592
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            593..729
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..852
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1042
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1155
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1374
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1557
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1803
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1946
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2103
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2309
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2389
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2545
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2690
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2843
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2958
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3158
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3317
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3417
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..5398
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4814..4819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /note="hexamer: AATAAA"
     polyA_site      4833
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /note="major polyA site"
     regulatory      5152..5157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
                     /note="hexamer: AATAAA"
     polyA_site      5171
                     /gene="LAMC2"
                     /gene_synonym="B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;
                     LAMB2T; LAMNB2"
ORIGIN      
        1 acagcggagc gcagagtgag aaccaccaac cgaggcgccg ggcagcgacc cctgcagcgg
       61 agacagagac tgagcggccc ggccccgcca tgcctgcgct ctggctgggc tgctgcctct
      121 gcttctcgct cctcctgccc gcagcccggg ccacctccag gagggaagtc tgtgattgca
      181 atgggaagtc caggcagtgt atctttgatc gggaacttca cagacaaact ggtaatggat
      241 tccgctgcct caactgcaat gacaacactg atggcattca ctgcgagaag tgcaagaatg
      301 gcttttaccg gcacagagaa agggaccgct gtttgccctg caattgtaac tccaaaggtt
      361 ctcttagtgc tcgatgtgac aactccggac ggtgcagctg taaaccaggt gtgacaggag
      421 ccagatgcga ccgatgtctg ccaggcttcc acatgctcac ggatgcgggg tgcacccaag
      481 accagagact gctagactcc aagtgtgact gtgacccagc tggcatcgca gggccctgtg
      541 acgcgggccg ctgtgtctgc aagccagctg tcactggaga acgctgtgat aggtgtcgat
      601 caggttacta taatctggat ggggggaacc ctgagggctg tacccagtgt ttctgctatg
      661 ggcattcagc cagctgccgc agctctgcag aatacagtgt ccataagatc acctctacct
      721 ttcatcaaga tgttgatggc tggaaggctg tccaacgaaa tgggtctcct gcaaagctcc
      781 aatggtcaca gcgccatcaa gatgtgttta gctcagccca acgactagac cctgtctatt
      841 ttgtggctcc tgccaaattt cttgggaatc aacaggtgag ctatggtcaa agcctgtcct
      901 ttgactaccg tgtggacaga ggaggcagac acccatctgc ccatgatgtg attctggaag
      961 gtgctggtct acggatcaca gctcccttga tgccacttgg caagacactg ccttgtgggc
     1021 tcaccaagac ttacacattc aggttaaatg agcatccaag caataattgg agcccccagc
     1081 tgagttactt tgagtatcga aggttactgc ggaatctcac agccctccgc atccgagcta
     1141 catatggaga atacagtact gggtacattg acaatgtgac cctgatttca gcccgccctg
     1201 tctctggagc cccagcaccc tgggttgaac agtgtatatg tcctgttggg tacaaggggc
     1261 aattctgcca ggattgtgct tctggctaca agagagattc agcgagactg gggccttttg
     1321 gcacctgtat tccttgtaac tgtcaagggg gaggggcctg tgatccagac acaggagatt
     1381 gttattcagg ggatgagaat cctgacattg agtgtgctga ctgcccaatt ggtttctaca
     1441 acgatccgca cgacccccgc agctgcaagc catgtccctg tcataacggg ttcagctgct
     1501 cagtgatgcc ggagacggag gaggtggtgt gcaataactg ccctcccggg gtcaccggtg
     1561 cccgctgtga gctctgtgct gatggctact ttggggaccc ctttggtgaa catggcccag
     1621 tgaggccttg tcagccctgt caatgcaaca acaatgtgga ccccagtgcc tctgggaatt
     1681 gtgaccggct gacaggcagg tgtttgaagt gtatccacaa cacagccggc atctactgcg
     1741 accagtgcaa agcaggctac ttcggggacc cattggctcc caacccagca gacaagtgtc
     1801 gagcttgcaa ctgtaacccc atgggctcag agcctgtagg atgtcgaagt gatggcacct
     1861 gtgtttgcaa gccaggattt ggtggcccca actgtgagca tggagcattc agctgtccag
     1921 cttgctataa tcaagtgaag attcagatgg atcagtttat gcagcagctt cagagaatgg
     1981 aggccctgat ttcaaaggct cagggtggtg atggagtagt acctgataca gagctggaag
     2041 gcaggatgca gcaggctgag caggcccttc aggacattct gagagatgcc cagatttcag
     2101 aaggtgctag cagatccctt ggtctccagt tggccaaggt gaggagccaa gagaacagct
     2161 accagagccg cctggatgac ctcaagatga ctgtggaaag agttcgggct ctgggaagtc
     2221 agtaccagaa ccgagttcgg gatactcaca ggctcatcac tcagatgcag ctgagcctgg
     2281 cagaaagtga agcttccttg ggaaacacta acattcctgc ctcagaccac tacgtggggc
     2341 caaatggctt taaaagtctg gctcaggagg ccacaagatt agcagaaagc cacgttgagt
     2401 cagccagtaa catggagcaa ctgacaaggg aaactgagga ctattccaaa caagccctct
     2461 cactggtgcg caaggccctg catgaaggag tcggaagcgg aagcggtagc ccggacggtg
     2521 ctgtggtgca agggcttgtg gaaaaattgg agaaaaccaa gtccctggcc cagcagttga
     2581 caagggaggc cactcaagcg gaaattgaag cagataggtc ttatcagcac agtctccgcc
     2641 tcctggattc agtgtctcgg cttcagggag tcagtgatca gtcctttcag gtggaagaag
     2701 caaagaggat caaacaaaaa gcggattcac tctcaagcct ggtaaccagg catatggatg
     2761 agttcaagcg tacacagaag aatctgggaa actggaaaga agaagcacag cagctcttac
     2821 agaatggaaa aagtgggaga gagaaatcag atcagctgct ttcccgtgcc aatcttgcta
     2881 aaagcagagc acaagaagca ctgagtatgg gcaatgccac tttttatgaa gttgagagca
     2941 tccttaaaaa cctcagagag tttgacctgc aggtggacaa cagaaaagca gaagctgaag
     3001 aagccatgaa gagactctcc tacatcagcc agaaggtttc agatgccagt gacaagaccc
     3061 agcaagcaga aagagccctg gggagcgctg ctgctgatgc acagagggca aagaatgggg
     3121 ccggggaggc cctggaaatc tccagtgaga ttgaacagga gattgggagt ctgaacttgg
     3181 aagccaatgt gacagcagat ggagccttgg ccatggaaaa gggactggcc tctctgaaga
     3241 gtgagatgag ggaagtggaa ggagagctgg aaaggaagga gctggagttt gacacgaata
     3301 tggatgcagt acagatggtg attacagaag cccagaaggt tgataccaga gccaagaacg
     3361 ctggggttac aatccaagac acactcaaca cattagacgg cctcctgcat ctgatggacc
     3421 agcctctcag tgtagatgaa gaggggctgg tcttactgga gcagaagctt tcccgagcca
     3481 agacccagat caacagccaa ctgcggccca tgatgtcaga gctggaagag agggcacgtc
     3541 agcagagggg ccacctccat ttgctggaga caagcataga tgggattctg gctgatgtga
     3601 agaacttgga gaacattagg gacaacctgc ccccaggctg ctacaatacc caggctcttg
     3661 agcaacagtg aagctgccat aaatatttct caactgaggt tcttgggata cagatctcag
     3721 ggctcgggag ccatgtcatg tgagtgggtg ggatggggac atttgaacat gtttaatggg
     3781 tatgctcagg tcaactgacc tgaccccatt cctgatccca tggccaggtg gttgtcttat
     3841 tgcaccatac tccttgcttc ctgatgctgg gcaatgaggc agatagcact gggtgtgaga
     3901 atgatcaagg atctggaccc caaagaatag actggatgga aagacaaact gcacaggcag
     3961 atgtttgcct cataatagtc gtaagtggag tcctggaatt tggacaagtg ctgttgggat
     4021 atagtcaact tattctttga gtaatgtgac taaaggaaaa aactttgact ttgcccaggc
     4081 atgaaattct tcctaatgtc agaacagagt gcaacccagt cacactgtgg ccagtaaaat
     4141 actattgcct catattgtcc tctgcaagct tcttgctgat cagagttcct cctacttaca
     4201 acccagggtg tgaacatgtt ctccattttc aagctggaag aagtgagcag tgttggagtg
     4261 aggacctgta aggcaggccc attcagagct atggtgcttg ctggtgcctg ccaccttcaa
     4321 gttctggacc tgggcatgac atcctttctt ttaatgatgc catggcaact tagagattgc
     4381 atttttatta aagcatttcc taccagcaaa gcaaatgttg ggaaagtatt tactttttcg
     4441 gtttcaaagt gatagaaaag tgtggcttgg gcattgaaag aggtaaaatt ctctagattt
     4501 attagtccta attcaatcct acttttcgaa caccaaaaat gatgcgcatc aatgtatttt
     4561 atcttatttt ctcaatctcc tctctctttc ctccacccat aataagagaa tgttcctact
     4621 cacacttcag ctgggtcaca tccatccctc cattcatcct tccatccatc tttccatcca
     4681 ttacctccat ccatccttcc aacatatatt tattgagtac ctactgtgtg ccaggggctg
     4741 gtgggacagt ggtgacatag tctctgccct catagagttg attgtctagt gaggaagaca
     4801 agcattttta aaaaataaat ttaaacttac aaactttgtt tgtcacaagt ggtgtttatt
     4861 gcaataaccg cttggtttgc aacctctttg ctcaacagaa catatgttgc aagaccctcc
     4921 catgggggca cttgagtttt ggcaaggctg acagagctct gggttgtgca catttctttg
     4981 cattccagct gtcactctgt gcctttctac aactgattgc aacagactgt tgagttatga
     5041 taacaccagt gggaattgct ggaggaacca gaggcacttc caccttggct gggaagacta
     5101 tggtgctgcc ttgcttctgt atttccttgg attttcctga aagtgttttt aaataaagaa
     5161 caattgttag atgcctgggg tcagtttctg atttactaaa ctctaagcct atgatggtac
     5221 caaagtaatg gaaatgaaaa tgccacagaa atggcaatag gtcctagagt tagcatcttc
     5281 agttcccact tctgagcatc ttacttcgcc cattccaacc atacaggatt gatgatgctg
     5341 tcaatgagat ttttatgagg tttaactgat gctgtcttct aagcataaag ctttattt
//
