LOCUS       NM_002293               7929 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens laminin subunit gamma 1 (LAMC1), mRNA.
ACCESSION   NM_002293
VERSION     NM_002293.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Zhao,L., Liu,S., Peng,Y. and Zhang,J.
  TITLE     Lamc1 promotes osteogenic differentiation and inhibits adipogenic
            differentiation of bone marrow-derived mesenchymal stem cells
  JOURNAL   Sci Rep 14 (1), 19592 (2024)
   PUBMED   39179716
  REMARK    GeneRIF: Lamc1 promotes osteogenic differentiation and inhibits
            adipogenic differentiation of bone marrow-derived mesenchymal stem
            cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Bai,J., Zhao,Y., Shi,K., Fan,Y., Ha,Y., Chen,Y., Luo,B., Lu,Y.,
            Jie,W. and Shen,Z.
  TITLE     HIF-1alpha-mediated LAMC1 overexpression is an unfavorable
            predictor of prognosis for glioma patients: evidence from
            pan-cancer analysis and validation experiments
  JOURNAL   J Transl Med 22 (1), 391 (2024)
   PUBMED   38678297
  REMARK    GeneRIF: HIF-1alpha-mediated LAMC1 overexpression is an unfavorable
            predictor of prognosis for glioma patients: evidence from
            pan-cancer analysis and validation experiments.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7929)
  AUTHORS   Chen,J., Liu,Z., Zhong,Y., Chen,H. and Xie,L.
  TITLE     Circ_0124208 Promotes the Progression of Hepatocellular Carcinoma
            by Regulating the miR-338-3p/LAMC1 Axis
  JOURNAL   Mol Biotechnol 65 (11), 1750-1763 (2023)
   PUBMED   36780058
  REMARK    GeneRIF: Circ_0124208 Promotes the Progression of Hepatocellular
            Carcinoma by Regulating the miR-338-3p/LAMC1 Axis.
REFERENCE   4  (bases 1 to 7929)
  AUTHORS   Jonnakuti,V.S., Ji,P., Gao,Y., Lin,A., Chu,Y., Elrod,N.,
            Huang,K.L., Li,W., Yalamanchili,H.K. and Wagner,E.J.
  TITLE     NUDT21 alters glioma migration through differential alternative
            polyadenylation of LAMC1
  JOURNAL   J Neurooncol 163 (3), 623-634 (2023)
   PUBMED   37389756
  REMARK    GeneRIF: NUDT21 alters glioma migration through differential
            alternative polyadenylation of LAMC1.
REFERENCE   5  (bases 1 to 7929)
  AUTHORS   Wang,L., Liu,Y., Gao,Q. and Hu,R.
  TITLE     Hsa_circ_0005085 may suppress cutaneous squamous cell carcinoma
            growth and metastasis through targeting the miR-186-5p/LAMC1 axis
  JOURNAL   Skin Res Technol 29 (6), e13321 (2023)
   PUBMED   37357644
  REMARK    GeneRIF: Hsa_circ_0005085 may suppress cutaneous squamous cell
            carcinoma growth and metastasis through targeting the
            miR-186-5p/LAMC1 axis.
REFERENCE   6  (bases 1 to 7929)
  AUTHORS   Aeschlimann,D. and Paulsson,M.
  TITLE     Cross-linking of laminin-nidogen complexes by tissue
            transglutaminase. A novel mechanism for basement membrane
            stabilization
  JOURNAL   J Biol Chem 266 (23), 15308-15317 (1991)
   PUBMED   1678389
REFERENCE   7  (bases 1 to 7929)
  AUTHORS   Kallunki,T., Ikonen,J., Chow,L.T., Kallunki,P. and Tryggvason,K.
  TITLE     Structure of the human laminin B2 chain gene reveals extensive
            divergence from the laminin B1 chain gene
  JOURNAL   J Biol Chem 266 (1), 221-228 (1991)
   PUBMED   1985895
REFERENCE   8  (bases 1 to 7929)
  AUTHORS   Santos,C.L., Sabbaga,J. and Brentani,R.
  TITLE     Differences in human laminin B2 sequences
  JOURNAL   DNA Seq 1 (4), 275-277 (1991)
   PUBMED   1806043
REFERENCE   9  (bases 1 to 7929)
  AUTHORS   Chung,A.E. and Durkin,M.E.
  TITLE     Entactin: structure and function
  JOURNAL   Am J Respir Cell Mol Biol 3 (4), 275-282 (1990)
   PUBMED   2119632
  REMARK    Review article
REFERENCE   10 (bases 1 to 7929)
  AUTHORS   Hunter,D.D., Shah,V., Merlie,J.P. and Sanes,J.R.
  TITLE     A laminin-like adhesive protein concentrated in the synaptic cleft
            of the neuromuscular junction
  JOURNAL   Nature 338 (6212), 229-234 (1989)
   PUBMED   2922051
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL450304.33, BC067813.1,
            BF507385.1, J03202.1, CN388644.1, BI768612.1, CF125114.1,
            DA401791.1 and AL354953.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002293.3.
            
            Summary: Laminins, a family of extracellular matrix glycoproteins,
            are the major noncollagenous constituent of basement membranes.
            They have been implicated in a wide variety of biological processes
            including cell adhesion, differentiation, migration, signaling,
            neurite outgrowth and metastasis. Laminins, composed of 3 non
            identical chains: laminin alpha, beta and gamma (formerly A, B1,
            and B2, respectively), have a cruciform structure consisting of 3
            short arms, each formed by a different chain, and a long arm
            composed of all 3 chains. Each laminin chain is a multidomain
            protein encoded by a distinct gene. Several isoforms of each chain
            have been described. Different alpha, beta and gamma chain isomers
            combine to give rise to different heterotrimeric laminin isoforms
            which are designated by Arabic numerals in the order of their
            discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The
            biological functions of the different chains and trimer molecules
            are largely unknown, but some of the chains have been shown to
            differ with respect to their tissue distribution, presumably
            reflecting diverse functions in vivo. This gene encodes the gamma
            chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought
            to be a beta chain, contains structural domains similar to beta
            chains, however, lacks the short alpha region separating domains I
            and II. The structural organization of this gene also suggested
            that it had diverged considerably from the beta chain genes.
            Embryos of transgenic mice in which both alleles of the gamma 1
            chain gene were inactivated by homologous recombination, lacked
            basement membranes, indicating that laminin, gamma 1 chain is
            necessary for laminin heterotrimer assembly. It has been inferred
            by analogy with the strikingly similar 3' UTR sequence in mouse
            laminin gamma 1 cDNA, that multiple polyadenylation sites are
            utilized in human to generate the 2 different sized mRNAs (5.5 and
            7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.1064429.1,
                                           SRR18074969.2576951.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258341.5/ ENSP00000258341.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AL450304.33        100279-100318
            41-470              BC067813.1         1-430
            471-880             BF507385.1         39-448
            881-1668            J03202.1           843-1630
            1669-1769           CN388644.1         541-641
            1770-2134           BI768612.1         83-447              c
            2135-2807           J03202.1           2097-2769
            2808-3239           CF125114.1         263-694
            3240-4424           J03202.1           3202-4386
            4425-4913           DA401791.1         51-539
            4914-7929           AL354953.16        48599-51614
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.3"
     gene            1..7929
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /note="laminin subunit gamma 1"
                     /db_xref="GeneID:3915"
                     /db_xref="HGNC:HGNC:6492"
                     /db_xref="MIM:150290"
     exon            1..715
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..5127
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /note="S-LAM gamma; laminin B2 chain; S-laminin subunit
                     gamma; laminin-2 subunit gamma; laminin-3 subunit gamma;
                     laminin-4 subunit gamma; laminin-6 subunit gamma;
                     laminin-7 subunit gamma; laminin-8 subunit gamma;
                     laminin-9 subunit gamma; laminin-10 subunit gamma;
                     laminin-11 subunit gamma; laminin, gamma 1 (formerly
                     LAMB2)"
                     /codon_start=1
                     /product="laminin subunit gamma-1 precursor"
                     /protein_id="NP_002284.3"
                     /db_xref="CCDS:CCDS1351.1"
                     /db_xref="GeneID:3915"
                     /db_xref="HGNC:HGNC:6492"
                     /db_xref="MIM:150290"
                     /translation="MRGSHRAAPALRPRGRLWPVLAVLAAAAAAGCAQAAMDECTDEG
                     GRPQRCMPEFVNAAFNVTVVATNTCGTPPEEYCVQTGVTGVTKSCHLCDAGQPHLQHG
                     AAFLTDYNNQADTTWWQSQTMLAGVQYPSSINLTLHLGKAFDITYVRLKFHTSRPESF
                     AIYKRTREDGPWIPYQYYSGSCENTYSKANRGFIRTGGDEQQALCTDEFSDISPLTGG
                     NVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYA
                     ISDFAVGGRCKCNGHASECMKNEFDKLVCNCKHNTYGVDCEKCLPFFNDRPWRRATAE
                     SASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGNN
                     EACSSCHCSPVGSLSTQCDSYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPS
                     GSIDECNIETGRCVCKDNVEGFNCERCKPGFFNLESSNPRGCTPCFCFGHSSVCTNAV
                     GYSVYSISSTFQIDEDGWRAEQRDGSEASLEWSSERQDIAVISDSYFPRYFIAPAKFL
                     GKQVLSYGQNLSFSFRVDRRDTRLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYV
                     FRLHEATDYPWRPALTPFEFQKLLNNLTSIKIRGTYSERSAGYLDDVTLASARPGPGV
                     PATWVESCTCPVGYGGQFCEMCLSGYRRETPNLGPYSPCVLCACNGHSETCDPETGVC
                     NCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPGGSSCAVVPKTKEVVCTNCPTG
                     TTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNT
                     AGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQ
                     DCGACDPGFYNLQSGQGCERCDCHALGSTNGQCDIRTGQCECQPGITGQHCERCEVNH
                     FGFGPEGCKPCDCHPEGSLSLQCKDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQE
                     CPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLRE
                     AQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIE
                     IASRELEKAKVAAANVSVTQPESTGDPNNMTLLAEEARKLAERHKQEADDIVRVAKTA
                     NDTSTEAYNLLLRTLAGENQTAFEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGD
                     KAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELE
                     VKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVN
                     DNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAV
                     QKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQ
                     AAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVS
                     DLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP"
     exon            716..1020
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1151
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1318
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1507
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1625
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1724
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1861
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1984
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2174
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2287
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2509
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2698
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..2944
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3098
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3241
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3420
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3577
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3783
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3863
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            3864..4001
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4002..4146
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4147..4296
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4297..4411
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4412..4611
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4612..4770
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4771..4870
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            4871..7929
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7901..7906
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /note="hexamer: AATAAA"
     polyA_site      7929
                     /gene="LAMC1"
                     /gene_synonym="LAMB2"
                     /note="major polyA site"
ORIGIN      
        1 gcactcgggc acgcgctcgg aagtcggggg tcggcgcgga gtgcaggctg ctcccggggt
       61 aggtgaggga agcgcggagg cggcgcgcgg gggcagtggt cggcgagcag cgcggtcctc
      121 gctaggggcg cccacccgtc agtctctccg gcgcgagccg ccgccaccgc ccgcgccgga
      181 gtcaggcccc tgggccccca ggctcaagca gcgaagcggc ctccggggga cgccgctagg
      241 cgagaggaac gcgccggtgc ccttgccttc gccgtgaccc agcgtgcggg cggcgggatg
      301 agagggagcc atcgggccgc gccggccctg cggccccggg ggcggctctg gcccgtgctg
      361 gccgtgctgg cggcggccgc cgcggcgggc tgtgcccagg cagccatgga cgagtgcacg
      421 gacgagggcg ggcggccgca gcgctgcatg cccgagttcg tcaacgccgc cttcaacgtg
      481 actgtggtgg ccaccaacac gtgtgggact ccgcccgagg aatactgtgt gcagaccggg
      541 gtgaccgggg tcaccaagtc ctgtcacctg tgcgacgccg ggcagcccca cctgcagcac
      601 ggggcagcct tcctgaccga ctacaacaac caggccgaca ccacctggtg gcaaagccag
      661 accatgctgg ccggggtgca gtaccccagc tccatcaacc tcacgctgca cctgggaaaa
      721 gcttttgaca tcacctatgt gcgtctcaag ttccacacca gccgcccgga gagctttgcc
      781 atttacaagc gcacacggga agacgggccc tggattcctt accagtacta cagtggttcc
      841 tgtgagaaca cctactccaa ggcaaaccgc ggcttcatca ggacaggagg ggacgagcag
      901 caggccttgt gtactgatga attcagtgac atttctcccc tcactggggg caacgtggcc
      961 ttttctaccc tggaaggaag gcccagcgcc tataactttg acaatagccc tgtgctgcag
     1021 gaatgggtaa ctgccactga catcagagta actcttaatc gcctgaacac ttttggagat
     1081 gaagtgttta acgatcccaa agttctcaag tcctattatt atgccatctc tgattttgct
     1141 gtaggtggca gatgtaaatg taatggacac gcaagcgagt gtatgaagaa cgaatttgat
     1201 aagctggtgt gtaattgcaa acataacaca tatggagtag actgtgaaaa gtgtcttcct
     1261 ttcttcaatg accggccgtg gaggagggca actgcggaaa gtgccagtga atgcctgccc
     1321 tgtgattgca atggtcgatc ccaggaatgc tacttcgacc ctgaactcta tcgttccact
     1381 ggccatgggg gccactgtac caactgccag gataacacag atggcgccca ctgtgagagg
     1441 tgccgagaga acttcttccg ccttggcaac aatgaagcct gctcttcatg ccactgtagt
     1501 cctgtgggct ctctaagcac acagtgtgat agttacggca gatgcagctg taagccagga
     1561 gtgatggggg acaaatgtga ccgttgccag cctggattcc attctctcac tgaagcagga
     1621 tgcaggccat gctcttgtga tccctctggc agcatagatg aatgtaatat tgaaacagga
     1681 agatgtgttt gcaaagacaa tgtcgaaggc ttcaattgtg aaagatgcaa acctggattt
     1741 tttaatctgg aatcatctaa tcctcggggt tgcacaccct gcttctgctt tgggcattct
     1801 tctgtctgta caaacgctgt tggctacagt gtttattcta tctcctctac ctttcagatt
     1861 gatgaggatg ggtggcgtgc ggaacagaga gatggctctg aagcatctct cgagtggtcc
     1921 tctgagaggc aagatatcgc cgtgatctca gacagctact ttcctcggta cttcattgct
     1981 cctgcaaagt tcttgggcaa gcaggtgttg agttatggtc agaacctctc cttctccttt
     2041 cgagtggaca ggcgagatac tcgcctctct gcagaagacc ttgtgcttga gggagctggc
     2101 ttaagagtat ctgtaccctt gatcgctcag ggcaattcct atccaagtga gaccactgtg
     2161 aagtatgtct tcaggctcca tgaagcaaca gattaccctt ggaggcctgc tcttacccct
     2221 tttgaatttc agaagctcct aaacaacttg acctctatca agatacgtgg gacatacagt
     2281 gagagaagtg ctggatattt ggatgatgtc accctggcaa gtgctcgtcc tgggcctgga
     2341 gtccctgcaa cttgggtgga gtcctgcacc tgtcctgtgg gatatggagg gcagttttgt
     2401 gagatgtgcc tctcaggtta cagaagagaa actcctaatc ttggaccata cagtccatgt
     2461 gtgctttgcg cctgcaatgg acacagcgag acctgtgatc ctgagacagg tgtttgtaac
     2521 tgcagagaca atacggctgg cccgcactgt gagaagtgca gtgatgggta ctatggagat
     2581 tcaactgcag gcacctcctc cgattgccaa ccctgtccgt gtcctggagg ttcaagttgt
     2641 gctgttgttc ccaagacaaa ggaggtggtg tgcaccaact gtcctactgg caccactggt
     2701 aagagatgtg agctctgtga tgatggctac tttggagacc ccctgggtag aaacggccct
     2761 gtgagacttt gccgcctgtg ccagtgcagt gacaacatcg atcccaatgc agttggaaat
     2821 tgcaatcgct tgacgggaga atgcctgaag tgcatctata acactgctgg cttctattgt
     2881 gaccggtgca aagacggatt ttttggaaat cccctggctc ccaatccagc agacaaatgc
     2941 aaagcctgca attgcaatct gtatgggacc atgaagcagc agagcagctg taaccccgtg
     3001 acggggcagt gtgaatgttt gcctcacgtg actggccagg actgtggtgc ttgtgaccct
     3061 ggattctaca atctgcagag tgggcaaggc tgtgagaggt gtgactgcca tgccttgggc
     3121 tccaccaatg ggcagtgtga catccgcacc ggccagtgtg agtgccagcc cggcatcact
     3181 ggtcagcact gtgagcgctg tgaggtcaac cactttgggt ttggacctga aggctgcaaa
     3241 ccctgtgact gtcatcctga gggatctctt tcacttcagt gcaaagatga tggtcgctgt
     3301 gaatgcagag aaggctttgt gggaaatcgc tgtgaccagt gtgaagaaaa ctatttctac
     3361 aatcggtctt ggcctggctg ccaggaatgt ccagcttgtt accggctggt aaaggataag
     3421 gttgctgatc atagagtgaa gctccaggaa ttagagagtc tcatagcaaa ccttggaact
     3481 ggggatgaga tggtgacaga tcaagccttc gaggatagac taaaggaagc agagagggaa
     3541 gttatggacc tccttcgtga ggcccaggat gtcaaagatg ttgaccagaa tttgatggat
     3601 cgcctacaga gagtgaataa cactctgtcc agccaaatta gccgtttaca gaatatccgg
     3661 aataccattg aagagactgg aaacttggct gaacaagcgc gtgcccatgt agagaacaca
     3721 gagcggttga ttgaaatcgc atccagagaa cttgagaaag caaaagtcgc tgctgccaat
     3781 gtgtcagtca ctcagccaga atctacaggg gacccaaaca acatgactct tttggcagaa
     3841 gaggctcgaa agcttgctga acgtcataaa caggaagctg atgacattgt tcgagtggca
     3901 aagacagcca atgatacgtc aactgaggca tacaacctgc ttctgaggac actggcagga
     3961 gaaaatcaaa cagcatttga gattgaagag cttaatagga agtatgaaca agcgaagaac
     4021 atctcacagg atctggaaaa acaagctgcc cgagtacatg aggaggccaa aagggccggt
     4081 gacaaagctg tggagatcta tgccagcgtg gctcagctga gccctttgga ctctgagaca
     4141 ctggagaatg aagcaaataa cataaagatg gaagctgaga atctggaaca actgattgac
     4201 cagaaattaa aagattatga ggacctcaga gaagatatga gagggaagga acttgaagtc
     4261 aagaaccttc tggagaaagg caagactgaa cagcagaccg cagaccaact cctagcccga
     4321 gctgatgctg ccaaggccct cgctgaagaa gctgcaaaga agggacggga taccttacaa
     4381 gaagctaatg acattctcaa caacctgaaa gattttgata ggcgtgtgaa cgataacaag
     4441 acggccgcag aggaggcact aaggaagatt cctgccatca accagaccat cactgaagcc
     4501 aatgaaaaga ccagagaagc ccagcaggcc ctgggcagtg ctgcggcgga tgccacagag
     4561 gccaagaaca aggcccatga ggcggagagg atcgcgagcg ctgtccaaaa gaatgccacc
     4621 agcaccaagg cagaagctga aagaactttt gcagaagtta cagatctgga taatgaggtg
     4681 aacaatatgt tgaagcaact gcaggaagca gaaaaagagc taaagagaaa acaagatgac
     4741 gctgaccagg acatgatgat ggcagggatg gcttcacagg ctgctcaaga agccgagatc
     4801 aatgccagaa aagccaaaaa ctctgttact agcctcctca gcattattaa tgacctcttg
     4861 gagcagctgg ggcagctgga tacagtggac ctgaataagc taaacgagat tgaaggcacc
     4921 ctaaacaaag ccaaagatga aatgaaggtc agcgatcttg ataggaaagt gtctgacctg
     4981 gagaatgaag ccaagaagca ggaggctgcc atcatggact ataaccgaga tatcgaggag
     5041 atcatgaagg acattcgcaa tctggaggac atcaggaaga ccttaccatc tggctgcttc
     5101 aacaccccgt ccattgaaaa gccctagtgt ctttagggct ggaaggcagc atccctctga
     5161 caggggggca gttgtgaggc cacagagtgc cttgacacaa agattacatt tttcagaccc
     5221 ccactcctct gctgctgtcc atgactgtcc ttttgaacca ggaaaagtca cagagtttaa
     5281 agagaagcaa attaaacatc ctgaatcggg aacaaagggt tttatctaat aaagtgtctc
     5341 ttccattcac gttgctacct tacccacact ttcccttctg atttgcgtga ggacgtggca
     5401 tcctacgtta ctgtacagtg gcataagcac atcgtgtgag cccatgtatg ctggggtaga
     5461 gcaagtagcc ctcccctgtc tcatcgatac cagcagaacc tcctcagtct cagtactctt
     5521 gtttctatga aggaaaagtt tggctactaa cagtagcatt gtgatggcca gtatatccag
     5581 tccatggata aagaaaatgc atctgcatct cctacccctc ttccttctaa gcaaaaggaa
     5641 ataaacatcc tgtgccaaag gtattggtca tttagaatgt cggtagccat ccatcagtgc
     5701 ttttagttat tatgagtgta ggacactgag ccatccgtgg gtcaggatgc aattatttat
     5761 aaaagtctcc aggtgaacat ggctgaagat ttttctagta tattaataat tgactaggaa
     5821 gatgaacttt ttttcagatc tttgggcagc tgataattta aatctggatg ggcagcttgc
     5881 actcaccaat agaccaaaag acatcttttg atattcttat aaatggaact tacacagaag
     5941 aaatagggat atgataacca ctaaaatttt gttttcaaaa tcaaactaat tcttacagct
     6001 tttttattag ttagtcttgg aactagtgtt aagtatctgg cagagaacag ttaatcccta
     6061 aggtcttgac aaaacagaag aaaaacaagc ctcctcgtcc tagtcttttc tagcaaaggg
     6121 ataaaactta gatggcagct tgtactgtca gaatcccgtg tatccatttg ttcttctgtt
     6181 ggagagatga gacatttgac ccttagctcc agttttcttc tgatgtttcc atcttccaga
     6241 atccctcaaa aaacattgtt tgccaaatcc tggtggcaaa tacttgcact cagtatttca
     6301 cacagctgcc aacgctatcg agttcctgca ctttgtgatt taaatccact ctaaaccttc
     6361 cctctaagtg tagagggaag acccttacgt ggagtttcct agtgggcttc tcaacttttg
     6421 atcctcagct ctgtggtttt aagaccacag tgtgacagtt ccctgccaca cacccccttc
     6481 ctcctaccaa cccacctttg agattcatat atagccttta acactatgca actttgtact
     6541 ttgcgtagca ggggcggggt ggggggaaag aaactattat ctgacacact ggtgctatta
     6601 attatttcaa atttatattt ttgtgtgaat gttttgtgtt ttgtttatca tgattataga
     6661 ataaggaatt tatgtaaata tacttagtcc tatttctaga atgacactct gttcactttg
     6721 ctcaattttt cctcttcact ggcacaatgt atctgaatac ctccttccct cccttctaga
     6781 attctttgga ttgtactcca aagaattgtg ccttgtgttt gcagcatctc cattctctaa
     6841 aattaatata attgctttcc tccacaccca gccactgtaa agaggtaact tgggtcctct
     6901 tccattgcag tcctgatgat cctaacctgc agcacggtgg ttttacaatg ttccagagca
     6961 ggaacgccag gttgacaagc tatggtagga ttaggaaagt ttgctgaaga ggatctttga
     7021 cgccacagtg ggactagcca ggaatgaggg agaaatgccc tttctggcaa ttgttggagc
     7081 tggataggta agttttataa gggagtacat tttgactgag cacttagggc atcaggaaca
     7141 gtgctactta ctgatgggta gactgggaga ggtggtgtaa cttagttctt gatgatccca
     7201 cttcctgttt ccatctgctt gggatatacc agagtttacc acaagtgttt tgacgatata
     7261 ctcctgagct ttcactctgc tgcttctccc aggcctcttc tactatggca ggagatgtgg
     7321 cgtgctgttg caaagttttc acgtcattgt ttcctggcta gttcatttca ttaagtggct
     7381 acatcctaac atatgcattt ggtcaaggtt gcagaagagg actgaagatt gactgccaag
     7441 ctagtttggg tgaagttcac tccagcaagt ctcaggccac aatggggtgg tttggtttgg
     7501 tttcctttta actttctttt tgttatttgc ttttctcctc cacctgtgtg gtatattttt
     7561 taagcagaat tttatttttt aaaataaaag gttctttaca agatgatacc ttaattacac
     7621 tcccgcaaca cagccattat tttattgtct agctccagtt atctgtattt tatgtaatgt
     7681 aattgacagg atggctgctg cagaatgctg gttgacacag ggattattat actgctattt
     7741 ttccctgaat ttttttcctt tgaattccaa ctgtggacct tttatatgtg ccttcacttt
     7801 agctgtttgc cttaatctct acagccttgc tctccggggt ggttaataaa atgcaacact
     7861 tggcattttt atgttttaag aaaaacagta ttttatttat aataaaatct gaatatttgt
     7921 aacccttta
//
