LOCUS       NM_002292               5692 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens laminin subunit beta 2 (LAMB2), mRNA.
ACCESSION   NM_002292
VERSION     NM_002292.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Paiz,F., Alawneh,I., Nigro,E. and Gonorazky,H.D.
  TITLE     Expanding the spectrum of LAMB2: Pierson syndrome associated with
            neuromuscular junction disorder in two patients
  JOURNAL   Neuromuscul Disord 39, 30-32 (2024)
   PUBMED   38723581
  REMARK    GeneRIF: Expanding the spectrum of LAMB2: Pierson syndrome
            associated with neuromuscular junction disorder in two patients.
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Alshamrani,A.A., Magliyah,M., Alkuraya,F.S., Alabdi,L.,
            Alfaadhel,T.A. and Alsulaiman,S.M.
  TITLE     Early-Onset Myopia and Retinal Detachment without Typical
            Microcoria or Severe Proteinuria due to a Novel LAMB2 Variant
  JOURNAL   Ophthalmol Retina 8 (2), 155-162 (2024)
   PUBMED   37678612
  REMARK    GeneRIF: Early-Onset Myopia and Retinal Detachment without Typical
            Microcoria or Severe Proteinuria due to a Novel LAMB2 Variant.
REFERENCE   3  (bases 1 to 5692)
  AUTHORS   Magliyah,M.S. and Alsulaiman,S.M.
  TITLE     Development of neovascular glaucoma after intraocular surgery in
            Pierson syndrome
  JOURNAL   Ophthalmic Genet 42 (3), 317-319 (2021)
   PUBMED   33554690
  REMARK    GeneRIF: Development of neovascular glaucoma after intraocular
            surgery in Pierson syndrome.
REFERENCE   4  (bases 1 to 5692)
  AUTHORS   Kikkawa,Y., Hashimoto,T., Takizawa,K., Urae,S., Masuda,H.,
            Matsunuma,M., Yamada,Y., Hamada,K., Nomizu,M., Liapis,H.,
            Hisano,M., Akioka,Y., Miura,K., Hattori,M., Miner,J.H. and
            Harita,Y.
  TITLE     Laminin beta2 variants associated with isolated nephropathy that
            impact matrix regulation
  JOURNAL   JCI Insight 6 (6), 145908 (2021)
   PUBMED   33749661
  REMARK    GeneRIF: Laminin beta2 variants associated with isolated
            nephropathy that impact matrix regulation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5692)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5692)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   7  (bases 1 to 5692)
  AUTHORS   Aeschlimann,D. and Paulsson,M.
  TITLE     Cross-linking of laminin-nidogen complexes by tissue
            transglutaminase. A novel mechanism for basement membrane
            stabilization
  JOURNAL   J Biol Chem 266 (23), 15308-15317 (1991)
   PUBMED   1678389
REFERENCE   8  (bases 1 to 5692)
  AUTHORS   Chung,A.E. and Durkin,M.E.
  TITLE     Entactin: structure and function
  JOURNAL   Am J Respir Cell Mol Biol 3 (4), 275-282 (1990)
   PUBMED   2119632
  REMARK    Review article
REFERENCE   9  (bases 1 to 5692)
  AUTHORS   Engvall,E., Earwicker,D., Haaparanta,T., Ruoslahti,E. and
            Sanes,J.R.
  TITLE     Distribution and isolation of four laminin variants; tissue
            restricted distribution of heterotrimers assembled from five
            different subunits
  JOURNAL   Cell Regul 1 (10), 731-740 (1990)
   PUBMED   2099832
REFERENCE   10 (bases 1 to 5692)
  AUTHORS   Hunter,D.D., Shah,V., Merlie,J.P. and Sanes,J.R.
  TITLE     A laminin-like adhesive protein concentrated in the synaptic cleft
            of the neuromuscular junction
  JOURNAL   Nature 338 (6212), 229-234 (1989)
   PUBMED   2922051
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC135506.3, BP354643.1,
            CN265778.1, S77512.1, CV814167.1, X79683.1 and CN265784.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002292.3.
            
            Summary: Laminins, a family of extracellular matrix glycoproteins,
            are the major noncollagenous constituent of basement membranes.
            They have been implicated in a wide variety of biological processes
            including cell adhesion, differentiation, migration, signaling,
            neurite outgrowth and metastasis. Laminins, composed of 3 non
            identical chains: laminin alpha, beta and gamma (formerly A, B1,
            and B2, respectively), form a cruciform structure consisting of 3
            short arms, each formed by a different chain, and a long arm
            composed of all 3 chains. Each laminin chain is a multidomain
            protein encoded by a distinct gene. Several isoforms of each chain
            have been described. Different alpha, beta and gamma chain isomers
            combine to give rise to different heterotrimeric laminin isoforms
            which are designated by Arabic numerals in the order of their
            discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The
            biological functions of the different chains and trimer molecules
            are largely unknown, but some of the chains have been shown to
            differ with respect to their tissue distribution, presumably
            reflecting diverse functions in vivo. This gene encodes the beta
            chain isoform laminin, beta 2. The beta 2 chain contains the 7
            structural domains typical of beta chains of laminin, including the
            short alpha region. However, unlike beta 1 chain, beta 2 has a more
            restricted tissue distribution. It is enriched in the basement
            membrane of muscles at the neuromuscular junctions, kidney
            glomerulus and vascular smooth muscle. Transgenic mice in which the
            beta 2 chain gene was inactivated by homologous recombination,
            showed defects in the maturation of neuromuscular junctions and
            impairment of glomerular filtration. Alternative splicing involving
            a non consensus 5' splice site (gc) in the 5' UTR of this gene has
            been reported. It was suggested that inefficient splicing of this
            first intron, which does not change the protein sequence, results
            in a greater abundance of the unspliced form of the transcript than
            the spliced form. The full-length nature of the spliced transcript
            is not known. [provided by RefSeq, Aug 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: S77512.1, SRR14038196.1459450.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305544.9/ ENSP00000307156.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC135506.3         63348-63351         c
            5-438               BP354643.1         2-435
            439-725             CN265778.1         353-639
            726-3197            S77512.1           695-3166
            3198-3896           CV814167.1         53-751
            3897-4418           X79683.1           3879-4400
            4419-5039           CN265784.1         65-685
            5040-5673           S77512.1           5009-5642
            5674-5692           X79683.1           5656-5674
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..5692
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /note="laminin subunit beta 2"
                     /db_xref="GeneID:3913"
                     /db_xref="HGNC:HGNC:6487"
                     /db_xref="MIM:150325"
     exon            1..259
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..5580
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /note="laminin S; S-LAM beta; laminin B1s chain; S-laminin
                     subunit beta; laminin, beta 2 (laminin S)"
                     /codon_start=1
                     /product="laminin subunit beta-2 precursor"
                     /protein_id="NP_002283.3"
                     /db_xref="CCDS:CCDS2789.1"
                     /db_xref="GeneID:3913"
                     /db_xref="HGNC:HGNC:6487"
                     /db_xref="MIM:150325"
                     /translation="MELTSRERGRGQPLPWELRLGLLLSVLAATLAQAPAPDVPGCSR
                     GSCYPATGDLLVGRADRLTASSTCGLNGPQPYCIVSHLQDEKKCFLCDSRRPFSARDN
                     PHSHRIQNVVTSFAPQRRAAWWQSENGIPAVTIQLDLEAEFHFTHLIMTFKTFRPAAM
                     LVERSADFGRTWHVYRYFSYDCGADFPGVPLAPPRHWDDVVCESRYSEIEPSTEGEVI
                     YRVLDPAIPIPDPYSSRIQNLLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYE
                     LVVRGNCFCYGHASECAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYRDLPWR
                     PAEDGHSHACRKCECHGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCELCRPF
                     FYRDPTKDLRDPAVCRSCDCDPMGSQDGGRCDSHDDPALGLVSGQCRCKEHVVGTRCQ
                     QCRDGFFGLSISDRLGCRRCQCNARGTVPGSTPCDPNSGSCYCKRLVTGRGCDRCLPG
                     HWGLSHDLLGCRPCDCDVGGALDPQCDEGTGQCHCRQHMVGRRCEQVQPGYFRPFLDH
                     LIWEAEDTRGQVLDVVERLVTPGETPSWTGSGFVRLQEGQTLEFLVASVPKAMDYDLL
                     LRLEPQVPEQWAELELIVQRPGPVPAHSLCGHLVPKDDRIQGTLQPHARYLIFPNPVC
                     LEPGISYKLHLKLVRTGGSAQPETPYSGPGLLIDSLVLLPRVLVLEMFSGGDAAALER
                     QATFERYQCHEEGLVPSKTSPSEACAPLLISLSTLIYNGALPCQCNPQGSLSSECNPH
                     GGQCLCKPGVVGRRCDLCAPGYYGFGPTGCQACQCSHEGALSSLCEKTSGQCLCRTGA
                     FGLRCDRCQRGQWGFPSCRPCVCNGHADECNTHTGACLGCRDHTGGEHCERCIAGFHG
                     DPRLPYGGQCRPCPCPEGPGSQRHFATSCHQDEYSQQIVCHCRAGYTGLRCEACAPGH
                     FGDPSRPGGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHHTEGPHCAHCKPGFHGQA
                     ARQSCHRCTCNLLGTNPQQCPSPDQCHCDPSSGQCPCLPNVQGPSCDRCAPNFWNLTS
                     GHGCQPCACHPSRARGPTCNEFTGQCHCRAGFGGRTCSECQELHWGDPGLQCHACDCD
                     SRGIDTPQCHRFTGHCSCRPGVSGVRCDQCARGFSGIFPACHPCHACFGDWDRVVQDL
                     AARTQRLEQRAQELQQTGVLGAFESSFWHMQEKLGIVQGIVGARNTSAASTAQLVEAT
                     EELRREIGEATEHLTQLEADLTDVQDENFNANHALSGLERDRLALNLTLRQLDQHLDL
                     LKHSNFLGAYDSIRHAHSQSAEAERRANTSALAVPSPVSNSASARHRTEALMDAQKED
                     FNSKHMANQRALGKLSAHTHTLSLTDINELVCGAPGDAPCATSPCGGAGCRDEDGQPR
                     CGGLSCNGAAATADLALGRARHTQAELQRALAEGGSILSRVAETRRQASEAQQRAQAA
                     LDKANASRGQVEQANQELQELIQSVKDFLNQEGADPDSIEMVATRVLELSIPASAEQI
                     QHLAGAIAERVRSLADVDAILARTVGDVRRAEQLLQDARRARSWAEDEKQKAETVQAA
                     LEEAQRAQGIAQGAIRGAVADTRDTEQTLYQVQERMAGAERALSSAGERARQLDALLE
                     ALKLKRAGNSLAASTAEETAGSAQGRAQEAEQLLRGPLGDQYQTVKALAERKAQGVLA
                     AQARAEQLRDEARDLLQAAQDKLQRLQELEGTYEENERALESKAAQLDGLEARMRSVL
                     QAINLQVQIYNTCQ"
     exon            260..432
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            433..568
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            569..642
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            643..831
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            832..895
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1098
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1219
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1408
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1588
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1701
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1781
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1914
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2073
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2201
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2334
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2527
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2671
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2903
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3067
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3292
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3510
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            3511..3607
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            3608..3980
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            3981..4165
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            4166..4407
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            4408..4756
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            4757..4964
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            4965..5106
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            5107..5283
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5443
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     exon            5444..5692
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5671..5676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /note="hexamer: AATAAA"
     polyA_site      5692
                     /gene="LAMB2"
                     /gene_synonym="LAMS; NPHS5; PIERS"
                     /note="major polyA site"
ORIGIN      
        1 gctcaaagtc gctggactct aagctgtcgg agggaccgct ggacagacct gggaactgac
       61 agagggcctg gagggaaaca ggccaaagac ccacaggcag agttgacacg gaaccccaaa
      121 gcaaggagga gggctcgggc ccgagaccgt tcacctcccc ttatccctgt tcccctcttc
      181 aggatggagc tgacctcaag ggaaagaggg aggggacagc ctctgccctg ggaacttcga
      241 ctgggcctac tgctaagcgt gctggctgcc acactggcac aggcccctgc cccggatgtg
      301 cctggctgtt ccaggggaag ctgctacccc gccacgggcg acctgctggt gggccgagct
      361 gacagactga ctgcctcatc cacttgtggc ctgaatggcc cccagcccta ctgcatcgtc
      421 agtcacctgc aggacgaaaa gaagtgcttc ctttgtgact cccggcgccc cttctctgct
      481 agagacaacc cacacagcca tcgcatccag aatgtagtca ccagctttgc accacagcgg
      541 cgggcagcct ggtggcagtc agagaatggt atccctgcgg tcaccatcca gctggacctg
      601 gaggctgagt ttcatttcac acacctcatt atgaccttca agacatttcg ccctgctgcc
      661 atgctggtgg aacgctcagc agactttggc cgcacctggc atgtgtaccg atatttctcc
      721 tatgactgtg gggctgactt cccaggagtc ccactagcac ccccacggca ctgggatgat
      781 gtagtctgtg agtcccgcta ctcagagatt gagccatcca ctgaaggcga ggtcatctat
      841 cgtgtgctgg accctgccat ccctatccca gacccctaca gctcacggat tcagaacctg
      901 ttgaagatca ccaacctacg ggtgaacctg actcgtctac acacgttggg agacaaccta
      961 ctcgacccac ggagggagat ccgagagaag tactactatg ccctctatga gctggttgta
     1021 cgtggcaact gcttctgcta cggacacgcc tcagagtgtg cacccgcccc aggggcacca
     1081 gcccatgctg agggcatggt gcacggagct tgcatctgca aacacaacac acgtggcctc
     1141 aactgcgagc agtgtcagga tttctatcgt gacctgccct ggcgtccggc tgaggacggc
     1201 catagtcatg cctgtaggaa gtgtgagtgc catgggcaca cccacagctg ccacttcgac
     1261 atggccgtat acctggcatc tggcaatgtg agtggaggtg tgtgtgatgg atgtcagcat
     1321 aacacagctg ggcgccactg tgagctctgt cggcccttct tctaccgtga cccaaccaag
     1381 gacctgcggg atccggctgt gtgccgctcc tgtgattgtg accccatggg ttctcaagac
     1441 ggtggtcgct gtgattccca tgatgaccct gcactgggac tggtctccgg ccagtgtcgc
     1501 tgcaaagaac atgtggtggg cactcgctgc cagcaatgcc gtgatggctt ctttgggctc
     1561 agcatcagtg accgtctggg ctgccggcga tgtcaatgta atgcacgggg cacagtgcct
     1621 gggagcactc cttgtgaccc caacagtgga tcctgttact gcaaacgtct agtgactgga
     1681 cgtggatgtg accgctgcct gcctggccac tggggcctga gccacgacct gctcggctgc
     1741 cgcccctgtg actgcgacgt gggtggtgct ttggatcccc agtgtgatga gggcacaggt
     1801 caatgccact gccgccagca catggttggg cgacgctgtg agcaggtgca acctggctac
     1861 ttccggccct tcctggacca cctaatttgg gaggctgagg acacccgagg gcaggtgctc
     1921 gatgtggtgg agcgcctggt gacccccggg gaaactccat cctggactgg ctcaggcttc
     1981 gtgcggctac aggaaggtca gaccctggag ttcctggtgg cctctgtgcc gaaggctatg
     2041 gactatgacc tgctgctgcg cttagagccc caggtccctg agcaatgggc agagttggaa
     2101 ctgattgtgc agcgtccagg gcctgtgcct gcccacagcc tgtgtgggca tttggtgccc
     2161 aaggatgatc gcatccaagg gactctgcaa ccacatgcca ggtacttgat atttcctaat
     2221 cctgtctgcc ttgagcctgg tatctcctac aagctgcatc tgaagctggt acggacaggg
     2281 ggaagtgccc agcctgagac tccctactct ggacctggcc tgctcattga ctcgctggtg
     2341 ctgctgcccc gtgtcctggt gctagagatg tttagtgggg gtgatgctgc tgccctggag
     2401 cgccaggcca cctttgaacg ctaccaatgc catgaggagg gtctggtgcc cagcaagact
     2461 tctccctctg aggcctgcgc acccctcctc atcagcctgt ccaccctcat ctacaatggt
     2521 gccctgccat gtcagtgcaa ccctcaaggt tcactgagtt ctgagtgcaa ccctcatggt
     2581 ggtcagtgcc tgtgcaagcc tggagtggtt gggcgccgct gtgacctctg tgcccctggc
     2641 tactatggct ttggccccac aggctgtcaa gcctgccagt gcagccacga gggggcactc
     2701 agcagtctct gtgaaaagac cagtgggcaa tgtctctgtc gaactggtgc ctttgggctt
     2761 cgctgtgacc gctgccagcg tggccagtgg ggattcccta gctgccggcc atgtgtctgc
     2821 aatgggcatg cagatgagtg caacacccac acaggcgctt gcctgggctg ccgtgatcac
     2881 acagggggtg agcactgtga aaggtgcatt gctggtttcc acggggaccc acggctgcca
     2941 tatgggggcc agtgccggcc ctgtccctgt cctgaaggcc ctgggagcca acggcacttt
     3001 gctacttctt gccaccagga tgaatattcc cagcagattg tgtgccactg ccgggcaggc
     3061 tatacggggc tgcgatgtga agcttgtgcc cctgggcact ttggggaccc atcaaggcca
     3121 ggtggccggt gccaactgtg tgagtgcagt gggaacattg acccaatgga tcctgatgcc
     3181 tgtgaccccc acacggggca atgcctgcgc tgtttacacc acacagaggg tccacactgt
     3241 gcccactgca agcctggctt ccatgggcag gctgcccgac agagctgtca ccgctgcaca
     3301 tgcaacctgc tgggcacaaa tccgcagcag tgcccatctc ctgaccagtg ccactgtgat
     3361 ccaagcagtg ggcagtgccc atgcctcccc aatgtccagg gccctagctg tgaccgctgt
     3421 gcccccaact tctggaacct caccagtggc catggttgcc agccttgtgc ctgccaccca
     3481 agccgggcca gaggccccac ctgcaacgag ttcacagggc agtgccactg ccgtgccggc
     3541 tttggagggc ggacttgttc tgagtgccaa gagctccact ggggagaccc tgggttgcag
     3601 tgccatgcct gtgattgtga ctctcgtgga atagatacac ctcagtgtca ccgcttcaca
     3661 ggtcactgca gctgccgccc aggggtgtct ggtgtgcgct gtgaccagtg tgcccgtggc
     3721 ttctcaggaa tctttcctgc ctgccatccc tgccatgcat gcttcgggga ttgggaccga
     3781 gtggtgcagg acttggcagc ccgtacacag cgcctagagc agcgggcgca ggagttgcaa
     3841 cagacgggtg tgctgggtgc ctttgagagc agcttctggc acatgcagga gaagctgggc
     3901 attgtgcagg gcatcgtagg tgcccgcaac acctcagccg cctccactgc acagcttgtg
     3961 gaggccacag aggagctgcg gcgtgaaatt ggggaggcca ctgagcacct gactcagctc
     4021 gaggcagacc tgacagatgt gcaagatgag aacttcaatg ccaaccatgc actaagtggt
     4081 ctggagcgag ataggcttgc acttaatctc acactgcggc agctcgacca gcatcttgac
     4141 ttgctcaaac attcaaactt cctgggtgcc tatgacagca tccggcatgc ccatagccag
     4201 tctgcagagg cagaacgtcg tgccaatacc tcagccctgg cagtacctag ccctgtgagc
     4261 aactcggcaa gtgctcggca tcggacagag gcactgatgg atgctcagaa ggaggacttc
     4321 aacagcaaac acatggccaa ccagcgggca cttggcaagc tctctgccca tacccacacc
     4381 ctgagcctga cagacataaa tgagctggtg tgtggggcac caggggatgc accctgtgct
     4441 acaagccctt gtgggggtgc cggctgtcga gatgaggatg ggcagccgcg ctgtgggggc
     4501 ctcagctgca atggggcagc ggctacagca gacctagcac tgggccgggc ccggcacaca
     4561 caggcagagc tgcagcgggc actggcagaa ggtggtagca tcctcagcag agtggctgag
     4621 actcgtcggc aggcaagcga ggcacagcag cgggcccagg cagccctgga caaggctaat
     4681 gcttccaggg gacaggtgga acaggccaac caggaacttc aagaacttat ccagagtgtg
     4741 aaggacttcc tcaaccagga gggggctgat cctgatagca ttgaaatggt ggccacacgg
     4801 gtgctagagc tctccatccc agcttcagct gagcagatcc agcacctggc gggtgcgatt
     4861 gcagagcgag tccggagcct ggcagatgtg gatgcgatcc tggcacgtac tgtaggagat
     4921 gtgcgtcgtg ccgagcagct actgcaggat gcacggcggg caaggagctg ggctgaggat
     4981 gagaaacaga aggcagagac agtacaggca gcactggagg aggcccagcg ggcacagggt
     5041 attgcccagg gtgccatccg gggggcagtg gctgacacac gggacacaga gcagaccctg
     5101 taccaggtac aggagaggat ggcaggtgca gagcgggcac tgagctctgc aggtgaaagg
     5161 gctcggcagt tggatgctct cctggaggct ctgaaattga aacgggcagg aaatagtctg
     5221 gcagcctcta cagcagaaga aacggcaggc agtgcccagg gtcgtgccca ggaggctgag
     5281 cagctgctac gcggtcctct gggtgatcag taccagacgg tgaaggccct agctgagcgc
     5341 aaggcccaag gtgtgctggc tgcacaggca agggcagaac aactgcggga tgaggctcgg
     5401 gacctgttgc aagccgctca ggacaagctg cagcggctac aggaattgga aggcacctat
     5461 gaggaaaatg agcgggcact ggagagtaag gcagcccagt tggacgggtt ggaggccagg
     5521 atgcgcagcg tgcttcaagc catcaacttg caggtgcaga tctacaacac ctgccagtga
     5581 cccctgccca aggcctaccc cagttcctag cactgcccca catgcatgtc tgcctatgca
     5641 ctgaagagct cttggcccgg cagggccccc aataaaccag tgtgaacccc ca
//
