LOCUS       NM_001105206            7228 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens laminin subunit alpha 4 (LAMA4), transcript variant 1,
            mRNA.
ACCESSION   NM_001105206
VERSION     NM_001105206.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Yu,J.J., Zhang,L.J., He,C.C., Cao,Y.F. and Yang,J.
  TITLE     [Research Progress on the Role of Laminin Subunit Alpha 4 in
            Diseases]
  JOURNAL   Zhongguo Yi Xue Ke Xue Yuan Xue Bao 45 (1), 92-100 (2023)
   PUBMED   36861161
  REMARK    GeneRIF: [Research Progress on the Role of Laminin Subunit Alpha 4
            in Diseases].
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Wang,M., Li,C., Liu,Y. and Wang,Z.
  TITLE     Effect of LAMA4 on Prognosis and Its Correlation with Immune
            Infiltration in Gastric Cancer
  JOURNAL   Biomed Res Int 2021, 6428873 (2021)
   PUBMED   34414238
  REMARK    GeneRIF: Effect of LAMA4 on Prognosis and Its Correlation with
            Immune Infiltration in Gastric Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7228)
  AUTHORS   Goddi,A., Carmona,A., Schroedl,L., White,J.M., Piron,M.J., De
            Leon,A., Casimiro,I., Hoffman,A., Gonzalez Porras,M.A., Brey,E.M.,
            Brady,M.J. and Cohen,R.N.
  TITLE     Laminin-alpha4 Is Upregulated in Both Human and Murine Models of
            Obesity
  JOURNAL   Front Endocrinol (Lausanne) 12, 698621 (2021)
   PUBMED   34394003
  REMARK    GeneRIF: Laminin-alpha4 Is Upregulated in Both Human and Murine
            Models of Obesity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7228)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 7228)
  AUTHORS   Xiao,S., Lux,M.L., Reeves,R., Hudson,T.J. and Fletcher,J.A.
  TITLE     HMGI(Y) activation by chromosome 6p21 rearrangements in
            multilineage mesenchymal cells from pulmonary hamartoma
  JOURNAL   Am J Pathol 150 (3), 901-910 (1997)
   PUBMED   9060828
REFERENCE   6  (bases 1 to 7228)
  AUTHORS   Richards,A., Al-Imara,L. and Pope,F.M.
  TITLE     The complete cDNA sequence of laminin alpha 4 and its relationship
            to the other human laminin alpha chains
  JOURNAL   Eur J Biochem 238 (3), 813-821 (1996)
   PUBMED   8706685
REFERENCE   7  (bases 1 to 7228)
  AUTHORS   Iivanainen,A., Sainio,K., Sariola,H. and Tryggvason,K.
  TITLE     Primary structure and expression of a novel human laminin alpha 4
            chain
  JOURNAL   FEBS Lett 365 (2-3), 183-188 (1995)
   PUBMED   7781776
REFERENCE   8  (bases 1 to 7228)
  AUTHORS   Ryan,M.C., Tizard,R., VanDevanter,D.R. and Carter,W.G.
  TITLE     Cloning of the LamA3 gene encoding the alpha 3 chain of the
            adhesive ligand epiligrin. Expression in wound repair
  JOURNAL   J Biol Chem 269 (36), 22779-22787 (1994)
   PUBMED   8077230
REFERENCE   9  (bases 1 to 7228)
  AUTHORS   Richards,A.J., al-Imara,L., Carter,N.P., Lloyd,J.C., Leversha,M.A.
            and Pope,F.M.
  TITLE     Localization of the gene (LAMA4) to chromosome 6q21 and isolation
            of a partial cDNA encoding a variant laminin A chain
  JOURNAL   Genomics 22 (1), 237-239 (1994)
   PUBMED   7959779
REFERENCE   10 (bases 1 to 7228)
  AUTHORS   Burgeson,R.E., Chiquet,M., Deutzmann,R., Ekblom,P., Engel,J.,
            Kleinman,H., Martin,G.R., Meneguzzi,G., Paulsson,M., Sanes,J. et
            al.
  TITLE     A new nomenclature for the laminins
  JOURNAL   Matrix Biol 14 (3), 209-211 (1994)
   PUBMED   7921537
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB210027.1 and Z99289.2.
            
            On May 17, 2019 this sequence version replaced NM_001105206.2.
            
            Summary: Laminins, a family of extracellular matrix glycoproteins,
            are the major noncollagenous constituent of basement membranes.
            They have been implicated in a wide variety of biological processes
            including cell adhesion, differentiation, migration, signaling,
            neurite outgrowth and metastasis. Laminins are composed of 3 non
            identical chains: laminin alpha, beta and gamma (formerly A, B1,
            and B2, respectively) and they form a cruciform structure
            consisting of 3 short arms, each formed by a different chain, and a
            long arm composed of all 3 chains. Each laminin chain is a
            multidomain protein encoded by a distinct gene. Several isoforms of
            each chain have been described. Different alpha, beta and gamma
            chain isomers combine to give rise to different heterotrimeric
            laminin isoforms which are designated by Arabic numerals in the
            order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is
            laminin 1. The biological functions of the different chains and
            trimer molecules are largely unknown, but some of the chains have
            been shown to differ with respect to their tissue distribution,
            presumably reflecting diverse functions in vivo. This gene encodes
            the alpha chain isoform laminin, alpha 4. The domain structure of
            alpha 4 is similar to that of alpha 3, both of which resemble
            truncated versions of alpha 1 and alpha 2, in that approximately
            1,200 residues at the N-terminus (domains IV, V and VI) have been
            lost. Laminin, alpha 4 contains the C-terminal G domain which
            distinguishes all alpha chains from the beta and gamma chains. The
            RNA analysis from adult and fetal tissues revealed developmental
            regulation of expression, however, the exact function of laminin,
            alpha 4 is not known. Tissue-specific utilization of alternative
            polyA-signal has been described in literature. Alternative splicing
            results in multiple transcript variants encoding distinct isoforms.
            [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB210027.1, SRR1660805.44964.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000230538.12/ ENSP00000230538.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6027              AB210027.1         1-6027
            6028-7228           Z99289.2           50993-52193         c
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21"
     gene            1..7228
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="laminin subunit alpha 4"
                     /db_xref="GeneID:3910"
                     /db_xref="HGNC:HGNC:6484"
                     /db_xref="MIM:600133"
     exon            1..109
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            110..445
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             251..5722
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; laminin alpha 4 chain; laminin, alpha 4"
                     /codon_start=1
                     /product="laminin subunit alpha-4 isoform 1 precursor"
                     /protein_id="NP_001098676.2"
                     /db_xref="CCDS:CCDS43491.1"
                     /db_xref="GeneID:3910"
                     /db_xref="HGNC:HGNC:6484"
                     /db_xref="MIM:600133"
                     /translation="MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGR
                     QDPPETSEPRVALGRLPPAAEKCNAGFFHTLSGECVPCDCNGNSNECLDGSGYCVHCQ
                     RNTTGEHCEKCLDGYIGDSIRGAPQFCQPCPCPLPHLANFAESCYRKNGAVRCICNEN
                     YAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTG
                     FKCERCAPGYYGDARIAKNCAVCNCGGGPCDSVTGECLEEGFEPPTGMDCPTISCDKC
                     VWDLTDDLRLAALSIEEGKSGVLSVSSGAAAHRHVNEINATIYLLKTKLSERENQYAL
                     RKIQINNAENTMKSLLSDVEELVEKENQASRKGQLVQKESMDTINHASQLVEQAHDMR
                     DKIQEINNKMLYYGEEHELSPKEISEKLVLAQKMLEEIRSRQPFFTQRELVDEEADEA
                     YELLSQAESWQRLHNETRTLFPVVLEQLDDYNAKLSDLQEALDQALNYVRDAEDMNRA
                     TAARQRDHEKQQERVREQMEVVNMSLSTSADSLTTPRLTLSELDDIIKNASGIYAEID
                     GAKSELQVKLSNLSNLSHDLVQEAIDHAQDLQQEANELSRKLHSSDMNGLVQKALDAS
                     NVYENIVNYVSEANETAEFALNTTDRIYDAVSGIDTQIIYHKDESENLLNQARELQAK
                     AESSSDEAVADTSRRVGGALARKSALKTRLSDAVKQLQAAERGDAQQRLGQSRLITEE
                     ANRTTMEVQQATAPMANNLTNWSQNLQHFDSSAYNTAVNSARDAVRNLTEVVPQLLDQ
                     LRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHSRTSMDDLK
                     AFTSLSLYMKPPVKRPELTETADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKD
                     VEIPLDSKPVSSWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFSGDDSL
                     LDLDPEDTVFYVGGVPSNFKLPTSLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPS
                     TSVPCARDKLAFTQSRAASYFFDGSGYAVVRDITRRGKFGQVTRFDIEVRTPADNGLI
                     LLMVNGSMFFRLEMRNGYLHVFYDFGFSGGPVHLEDTLKKAQINDAKYHEISIIYHND
                     KKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRALRAHLPLDINFRGCMKG
                     FQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSFIASIQKISFFDGFEGGFN
                     FRTLQPNGLLFYYASGSDVFSISLDNGTVIMDVKGIKVQSVDKQYNDGLSHFVISSVS
                     PTRYELIVDKSRVGSKNPTKGKIEQTQASEKKFYFGGSPISAQYANFTGCISNAYFTR
                     VDRDVEVEDFQRYTEKVHTSLYECPIESSPLFLLHKKGKNLSKPKASQNKKGGKSKDA
                     PSWDPVALKLPERNTPRNSHCHLSNSPRAIEHAYQYGGTANSRQEFEHLKGDFGAKSQ
                     FSIRLRTRSSHGMIFYVSDQEENDFMTLFLAHGRLVYMFNVGHKKLKIRSQEKYNDGL
                     WHDVIFIRERSSGRLVIDGLRVLEESLPPTEATWKIKGPIYLGGVAPGKAVKNVQINS
                     IYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGL
                     KFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHMKNGQVIVKVNNGIRDFSTSVTPKQSL
                     CDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPIDHREPVFVGGVPESLLTPRLA
                     PSKPFTGCIRHFVIDGHPVSFSKAALVSGAVSINSCPAA"
     exon            446..547
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            548..672
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            673..753
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            754..968
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1064
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1216
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1327
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1439
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1607
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1801
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1918
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2067
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2209
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2306
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2423
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2603
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2743
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2917
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3063
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3226
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3227..3360
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..3532
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..3664
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3665..3807
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3808..3946
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..4084
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4085..4218
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4383
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4384..4537
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4538..4725
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4726..4915
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4916..5071
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5072..5231
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5232..5362
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5363..5456
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5457..5576
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5577..7228
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6025..6030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="hexamer: AATAAA"
     polyA_site      6030
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="major polyA site"
     regulatory      6271..6276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="hexamer: AGTAAA"
     polyA_site      6288
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
     regulatory      6381..6386
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
                     /note="hexamer: AATAAA"
     polyA_site      6402
                     /gene="LAMA4"
                     /gene_synonym="CMD1JJ; LAMA3; LAMA4*-1"
ORIGIN      
        1 gagaaggaag ccaggacagc gcagtcccca gtcccgaacg gccagggaga ggaggtggcc
       61 tagcgctggc ggggctcacc ccaatccgtc tgccttttga tgccgtactc tgctggttgc
      121 gcagccacct cgggatactg cacacggaga ggagggaaaa taagcgaggc accgccgcac
      181 cacgcgggag acctacggag acccacagcg cccgagccct ggaagagcac tactggatgt
      241 cagcggagaa atggctttga gctcagcctg gcgctcggtt ctgcctctgt ggctcctctg
      301 gagcgctgcc tgctcccgcg ccgcgtccgg ggacgacaac gcttttcctt ttgacattga
      361 agggagctca gcggttggca ggcaagaccc gcctgagacg agcgaacccc gcgtggctct
      421 gggacgcctg ccgcctgcgg ccgagaaatg caatgctgga ttctttcaca ccctgtcggg
      481 agaatgtgtg ccctgcgact gtaatggcaa ttccaacgag tgtttggacg gctcaggata
      541 ctgtgtgcac tgccagcgga acacaacagg agagcactgt gaaaagtgtc tggatggtta
      601 tatcggagat tccatcaggg gagcacccca attctgccag ccgtgcccct gtcccctgcc
      661 ccacttggcc aattttgcag aatcctgcta taggaaaaat ggagctgttc ggtgcatttg
      721 taacgaaaat tatgctggac ctaactgtga aagatgtgct cccggttact atggaaaccc
      781 cttactcatt ggaagcacct gtaagaaatg tgactgcagt ggaaattcag atcccaacct
      841 gatctttgaa gattgtgatg aagtcactgg ccagtgtagg aattgcttac gcaacaccac
      901 cggattcaag tgtgaacgtt gcgctcctgg ctactatggg gacgccagga tagccaagaa
      961 ctgtgcagtg tgcaactgcg ggggaggccc atgtgacagt gtaaccggag aatgcttgga
     1021 agaaggtttt gaacccccta caggcatgga ctgcccaacc ataagctgtg ataagtgcgt
     1081 ctgggacctg actgatgacc tgcggttagc agcgctctcc atcgaggaag gcaaatccgg
     1141 ggtgctgagc gtatcctctg gggccgccgc tcataggcac gtgaatgaaa tcaacgccac
     1201 catctacctc ctcaaaacaa aattgtcaga aagagaaaac caatacgccc taagaaagat
     1261 acaaatcaac aatgctgaga acacgatgaa aagccttctg tctgacgtag aggaattagt
     1321 tgaaaaggaa aatcaagcct ccagaaaagg acaacttgtt cagaaggaaa gcatggacac
     1381 cattaaccac gcaagtcagc tggtagagca agcccatgat atgagggata aaatccaaga
     1441 gatcaacaac aagatgctct attatgggga agagcatgaa cttagcccca aggaaatctc
     1501 tgagaagctg gtgttggccc agaagatgct tgaagagatt agaagccgtc aaccattttt
     1561 cacccaacgg gagctcgtgg atgaggaggc agatgaggct tacgaactac tgagccaggc
     1621 tgagagctgg cagcggctgc acaatgagac ccgcactctg tttcctgtcg tcctggagca
     1681 gctggatgac tacaatgcta agttgtcaga tctccaggaa gcacttgacc aggcccttaa
     1741 ctatgtcagg gatgccgaag acatgaacag ggccacagca gccaggcagc gggaccatga
     1801 gaaacaacag gaaagagtga gggaacaaat ggaagtggtg aacatgtctc tgagcacatc
     1861 tgcggactct ctgacaacac ctcgtctaac tctttcagaa cttgatgata taataaagaa
     1921 tgcgtcaggg atttatgcag aaatagatgg agccaaaagt gaactacaag taaaactatc
     1981 taacctaagt aacctcagcc atgatttagt ccaagaagct attgaccatg cacaggacct
     2041 tcaacaagaa gctaatgaat tgagcaggaa gttgcacagt tcagatatga acgggctggt
     2101 acagaaggct ttggatgcat caaatgtcta tgaaaatatt gttaattatg ttagtgaagc
     2161 caatgaaaca gcagaatttg ctttgaacac cactgaccga atttatgatg cggtgagtgg
     2221 gattgatact caaatcattt accataaaga tgaaagtgag aacctcctca atcaagccag
     2281 agaactgcaa gcaaaggcag agtctagcag tgatgaagca gtggctgaca ctagcaggcg
     2341 tgtgggtgga gccctagcaa ggaaaagtgc ccttaaaacc agactcagtg atgccgttaa
     2401 gcaactacaa gcagcagaga gaggggatgc ccagcagcgc ctggggcagt ctagactgat
     2461 caccgaggaa gccaacagga cgacgatgga ggtgcagcag gccactgccc ccatggccaa
     2521 caatctaacc aactggtcac agaatcttca acattttgac tcttctgctt acaacactgc
     2581 agtgaactct gctagggatg cagtaagaaa tctgaccgag gttgtccctc agctcctgga
     2641 tcagcttcgt acggttgagc agaagcgacc tgcaagcaac gtttctgcca gcatccagag
     2701 gatccgagag ctcattgctc agaccagaag tgttgccagc aagatccaag tctccatgat
     2761 gtttgatggc cagtcagctg tggaagtgca ctcgagaacc agtatggatg acttaaaggc
     2821 cttcacgtct ctgagcctgt acatgaaacc ccctgtgaag cggccggaac tgaccgagac
     2881 tgcagatcag tttatcctgt acctcggaag caaaaacgcc aaaaaagagt atatgggtct
     2941 tgcaatcaaa aatgataatc tggtatacgt ctataatttg ggaactaaag atgtggagat
     3001 tcccctggac tccaagcccg tcagttcctg gcctgcttac ttcagcattg tcaagattga
     3061 aagggtggga aaacatggaa aggtgttttt aacagtcccg agtctaagta gcacagcaga
     3121 ggaaaagttc attaaaaagg gggaattttc gggagatgac tctctgctgg acctggaccc
     3181 tgaggacaca gtgttttatg ttggtggagt gccttccaac ttcaagctcc ctaccagctt
     3241 aaacctgcct ggctttgttg gctgcctgga actggccact ttgaataatg atgtgatcag
     3301 cttgtacaac tttaagcaca tctataatat ggacccctcc acatcagtgc catgtgcccg
     3361 agataagctg gccttcactc agagtcgggc tgccagttac ttcttcgatg gctccggtta
     3421 tgccgtggtg agagacatca caaggagagg gaaatttggt caggtgactc gctttgacat
     3481 agaagttcga acaccagctg acaacggcct tattctcctg atggtcaatg gaagtatgtt
     3541 tttcagactg gaaatgcgca atggttacct acatgtgttc tatgattttg gattcagcgg
     3601 tggccctgtg catcttgaag atacgttaaa gaaagctcaa attaatgatg caaaatacca
     3661 tgagatctca atcatttacc acaatgataa gaaaatgatc ttggtagttg acagaaggca
     3721 tgtcaagagc atggataatg aaaagatgaa aatacctttt acagatatat acattggagg
     3781 agctcctcca gaaatcttac aatccagggc cctcagagca caccttcccc tagatatcaa
     3841 cttcagagga tgcatgaagg gcttccagtt ccaaaagaag gacttcaatt tactggagca
     3901 gacagaaacc ctgggagttg gttatggatg cccagaagac tcacttatat ctcgcagagc
     3961 atatttcaat ggacagagct tcattgcttc aattcagaaa atatctttct ttgatggctt
     4021 tgaaggaggt tttaatttcc gaacattaca accaaatggg ttactattct attatgcttc
     4081 agggtcagac gtgttctcca tctcactgga taatggtact gtcatcatgg atgtaaaggg
     4141 aatcaaagtt cagtcagtag ataagcagta caatgatggg ctgtcccact tcgtcattag
     4201 ctctgtctca cccacaagat atgaactgat agtagataaa agcagagttg ggagtaagaa
     4261 tcctaccaaa gggaaaatag aacagacaca agcaagtgaa aagaagtttt acttcggtgg
     4321 ctcaccaatc agtgctcagt atgctaattt cactggctgc ataagtaatg cctactttac
     4381 cagggtggat agagatgtgg aggttgaaga tttccaacgg tatactgaaa aggtccacac
     4441 ttctctttat gagtgtccca ttgagtcttc accattgttt ctcctccata aaaaaggaaa
     4501 aaatttatcc aagcctaaag caagtcagaa taaaaaggga gggaaaagta aagatgcacc
     4561 ttcatgggat cctgttgctc tgaaactccc agagcggaat actccaagaa actctcattg
     4621 ccacctttcc aacagcccta gagcaataga gcacgcctat caatatggag gaacagccaa
     4681 cagccgccaa gagtttgaac acttaaaagg agattttggt gccaaatctc agttttccat
     4741 tcgtctgaga actcgttcct cccatggcat gatcttctat gtctcagatc aagaagagaa
     4801 tgacttcatg actctatttt tggcccatgg ccgcttggtt tacatgttta atgttggtca
     4861 caaaaaactg aagattagaa gccaggagaa atacaatgat ggcctgtggc atgatgtgat
     4921 atttattcga gaaaggagca gtggccgact ggtaattgat ggtctccgag tcctagaaga
     4981 aagtcttcct cctactgaag ctacctggaa aatcaagggt cccatttatt tgggaggtgt
     5041 ggctcctgga aaggctgtga aaaatgttca gattaactcc atctacagtt ttagtggctg
     5101 tctcagcaat ctccagctca atggggcctc catcacctct gcttctcaga cattcagtgt
     5161 gaccccttgc tttgaaggcc ccatggaaac aggaacttac ttttcaacag aaggaggata
     5221 cgtggttcta gatgaatctt tcaatattgg attgaagttt gaaattgcat ttgaagtccg
     5281 tcccagaagc agttccggaa ccctggtcca cggccacagt gtcaatgggg agtacctaaa
     5341 tgttcacatg aaaaatggac aggtcatagt gaaagtcaat aatggcatca gagatttttc
     5401 cacctcagtt acacccaagc agagtctctg tgatggcaga tggcacagaa ttacagttat
     5461 tagagattct aatgtggttc agttggatgt ggactctgaa gtgaaccatg tggttggacc
     5521 cctgaatcca aaaccaattg atcacaggga gcctgtgttt gttggaggtg ttccagaatc
     5581 tctactgaca ccacgcttgg cccccagcaa acccttcaca ggctgcatac gccactttgt
     5641 gattgatgga cacccagtga gcttcagtaa agcagccctg gtcagcggcg ccgtaagcat
     5701 caactcctgt ccagcagcct gacatgacag agcacagctg cccaaataca aagttcttta
     5761 gagcactgaa agaaacacaa agccagccag gaggaacagt aactcttcct tcgggtggaa
     5821 gctttcatcg agttgaacag gacttaaacg aatcatcagg gaccggatat ttcttatttc
     5881 tcatttggat tcttaacctt gaatccaaag tgtctgcaat ggacaacaat tgaaggagtg
     5941 gcaaacttac ttgtattgag agcacacgca attcctactg gtgaaattac tgtttctgtt
     6001 tctaataaaa tagaagggat tccaaataaa cacttgcaca catttttgaa gtgcggctag
     6061 attctcagat tcacctttct tccagggaag ataactttca atctatataa aaatctctgt
     6121 cctaaaacta cctttcttta ttttgaagag acttactaac ttacatataa tctaaattag
     6181 atgatagatt tgtttttagc ccttttgttt ggtctatcag tataagaaga atattttagg
     6241 tttatagctg aagttatcaa ggtttaataa agtaaatttc taacagaata ctagaaaaat
     6301 gcagtataat ttaatttttt ctaaataaga aacacaggaa atcaactact ttttcccctt
     6361 ccttatctcc ttaaaagaaa aataaaattg tacatgagag gaggcttctg taggttatta
     6421 ttaccattat tgtgtgttct atgggaatca ttgaggatat cacagcaaaa acagtaggac
     6481 aaaatcataa aattcaattt aagagtacac aagtcctttt attaaaagtt tgctcctagc
     6541 ctgggcaaca taatgagatc ccatctctgc aaaaaaattt gtacatgggc atacacctgt
     6601 agtcccagct acttgggagg ctgagacggg aggatcgctt aagctcagga gttcaaggct
     6661 gcagtgagct atgactgctg actgtacctg cactccagcc tgggcaacag agtgagatcc
     6721 tgtctcaaaa acaaagtgtg ctctccacat acctgcaaca caactagtct tatttctaaa
     6781 atgttataat cttttttcca agtagctaca ttaatatagt ctagaaaaaa atggacttga
     6841 atagctggta gaatattaaa atatagaaat gaaataaaag aattatatct aaaaacctca
     6901 actcagaaga cagaaaaaga gaaaataggc cctgatatca acagaattaa caatacataa
     6961 aaggagtaac ttttgagggg agaggatata aaatattttg aggaattacc aaggggaata
     7021 aaacaatgtt accttgaaat gattatatat atattacata ttggtatata tgtccatacc
     7081 tacctatatc ccctgctacc cttctgtctg aaatatacaa ataatgataa tgttgaagat
     7141 atcgataaac atagctaatg tctgttcata gaggacttac taagtgccag ccaccatgat
     7201 aagctaaagt taattatttt atttgttc
//
