LOCUS       NM_002286               1976 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens lymphocyte activating 3 (LAG3), transcript variant 1,
            mRNA.
ACCESSION   NM_002286
VERSION     NM_002286.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Wakamatsu,E., Machiyama,H., Toyota,H., Takeuchi,A., Hashimoto,R.,
            Kozono,H. and Yokosuka,T.
  TITLE     Indirect suppression of CD4 T cell activation through
            LAG-3-mediated trans-endocytosis of MHC class II
  JOURNAL   Cell Rep 43 (9), 114655 (2024)
   PUBMED   39191259
  REMARK    GeneRIF: Indirect suppression of CD4 T cell activation through
            LAG-3-mediated trans-endocytosis of MHC class II.
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Perales,O., Jilaveanu,L., Adeniran,A., Su,D.G., Hurwitz,M.,
            Braun,D.A., Kluger,H.M. and Schoenfeld,D.A.
  TITLE     TIGIT expression in renal cell carcinoma infiltrating T cells is
            variable and inversely correlated with PD-1 and LAG3
  JOURNAL   Cancer Immunol Immunother 73 (10), 192 (2024)
   PUBMED   39105820
  REMARK    GeneRIF: TIGIT expression in renal cell carcinoma infiltrating T
            cells is variable and inversely correlated with PD-1 and LAG3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1976)
  AUTHORS   Chen,H., Molberg,K., Carrick,K., Niu,S., Rivera Colon,G., Gwin,K.,
            Lewis,C., Lea,J., Panwar,V., Zheng,W., Castrillon,D.H. and Lucas,E.
  TITLE     Expression and Prognostic Significance of LAG-3, TIGIT, VISTA, and
            IDO1 in Endometrial Serous Carcinoma
  JOURNAL   Mod Pathol 37 (8), 100532 (2024)
   PUBMED   38848896
  REMARK    GeneRIF: Expression and Prognostic Significance of LAG-3, TIGIT,
            VISTA, and IDO1 in Endometrial Serous Carcinoma.
REFERENCE   4  (bases 1 to 1976)
  AUTHORS   Saevarsdottir,S., Bjarnadottir,K., Markusson,T., Berglund,J.,
            Olafsdottir,T.A., Halldorsson,G.H., Rutsdottir,G.,
            Gunnarsdottir,K., Arnthorsson,A.O., Lund,S.H., Stefansdottir,L.,
            Gudmundsson,J., Johannesson,A.J., Sturluson,A., Oddsson,A.,
            Halldorsson,B., Ludviksson,B.R., Ferkingstad,E., Ivarsdottir,E.V.,
            Sveinbjornsson,G., Grondal,G., Masson,G., Eldjarn,G.H.,
            Thorisson,G.A., Kristjansdottir,K., Knowlton,K.U., Moore,K.H.S.,
            Gudjonsson,S.A., Rognvaldsson,S., Knight,S., Nadauld,L.D., Holm,H.,
            Magnusson,O.T., Sulem,P., Gudbjartsson,D.F., Rafnar,T.,
            Thorleifsson,G., Melsted,P., Norddahl,G.L., Jonsdottir,I. and
            Stefansson,K.
  TITLE     Start codon variant in LAG3 is associated with decreased LAG-3
            expression and increased risk of autoimmune thyroid disease
  JOURNAL   Nat Commun 15 (1), 5748 (2024)
   PUBMED   38982041
  REMARK    GeneRIF: Start codon variant in LAG3 is associated with decreased
            LAG-3 expression and increased risk of autoimmune thyroid disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1976)
  AUTHORS   Mao,X., Gu,H., Kim,D., Kimura,Y., Wang,N., Xu,E., Kumbhar,R.,
            Ming,X., Wang,H., Chen,C., Zhang,S., Jia,C., Liu,Y., Bian,H.,
            Karuppagounder,S.S., Akkentli,F., Chen,Q., Jia,L., Hwang,H.,
            Lee,S.H., Ke,X., Chang,M., Li,A., Yang,J., Rastegar,C.,
            Sriparna,M., Ge,P., Brahmachari,S., Kim,S., Zhang,S., Shimoda,Y.,
            Saar,M., Liu,H., Kweon,S.H., Ying,M., Workman,C.J., Vignali,D.A.A.,
            Muller,U.C., Liu,C., Ko,H.S., Dawson,V.L. and Dawson,T.M.
  TITLE     Aplp1 interacts with Lag3 to facilitate transmission of pathologic
            alpha-synuclein
  JOURNAL   Nat Commun 15 (1), 4663 (2024)
   PUBMED   38821932
  REMARK    GeneRIF: Aplp1 interacts with Lag3 to facilitate transmission of
            pathologic alpha-synuclein.
            Erratum:[Nat Commun. 2024 Jul 30;15(1):6413. doi:
            10.1038/s41467-024-50640-2.. PMID: 39080298]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1976)
  AUTHORS   Huard,B., Mastrangeli,R., Prigent,P., Bruniquel,D., Donini,S.,
            El-Tayar,N., Maigret,B., Dreano,M. and Triebel,F.
  TITLE     Characterization of the major histocompatibility complex class II
            binding site on LAG-3 protein
  JOURNAL   Proc Natl Acad Sci U S A 94 (11), 5744-5749 (1997)
   PUBMED   9159144
REFERENCE   7  (bases 1 to 1976)
  AUTHORS   Bruniquel,D., Borie,N. and Triebel,F.
  TITLE     Genomic organization of the human LAG-3/CD4 locus
  JOURNAL   Immunogenetics 47 (1), 96-98 (1997)
   PUBMED   9382927
REFERENCE   8  (bases 1 to 1976)
  AUTHORS   Blum,M.D., Wong,G.T., Higgins,K.M., Sunshine,M.J. and Lacy,E.
  TITLE     Reconstitution of the subclass-specific expression of CD4 in
            thymocytes and peripheral T cells of transgenic mice:
            identification of a human CD4 enhancer
  JOURNAL   J Exp Med 177 (5), 1343-1358 (1993)
   PUBMED   8097522
REFERENCE   9  (bases 1 to 1976)
  AUTHORS   Baixeras,E., Huard,B., Miossec,C., Jitsukawa,S., Martin,M.,
            Hercend,T., Auffray,C., Triebel,F. and Piatier-Tonneau,D.
  TITLE     Characterization of the lymphocyte activation gene 3-encoded
            protein. A new ligand for human leukocyte antigen class II antigens
  JOURNAL   J Exp Med 176 (2), 327-337 (1992)
   PUBMED   1380059
REFERENCE   10 (bases 1 to 1976)
  AUTHORS   Triebel,F., Jitsukawa,S., Baixeras,E., Roman-Roman,S., Genevee,C.,
            Viegas-Pequignot,E. and Hercend,T.
  TITLE     LAG-3, a novel lymphocyte activation gene closely related to CD4
  JOURNAL   J Exp Med 171 (5), 1393-1405 (1990)
   PUBMED   1692078
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC125494.2, X51985.3,
            AI274900.1 and AA687676.1.
            
            On Nov 23, 2018 this sequence version replaced NM_002286.5.
            
            Summary: Lymphocyte-activation protein 3 belongs to Ig superfamily
            and contains 4 extracellular Ig-like domains. The LAG3 gene
            contains 8 exons.  The sequence data, exon/intron organization, and
            chromosomal localization all indicate a close relationship of LAG3
            to CD4. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.544442.1, AK292104.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000203629.3/ ENSP00000203629.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AC125494.2         25793-25895         c
            104-1696            X51985.3           1-1593
            1697-1974           AI274900.1         1-278               c
            1975-1976           AA687676.1         4-5                 c
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..1976
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /note="lymphocyte activating 3"
                     /db_xref="GeneID:3902"
                     /db_xref="HGNC:HGNC:6476"
                     /db_xref="MIM:153337"
     exon            1..391
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     CDS             334..1911
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; lymphocyte activation gene 3 protein;
                     lymphocyte-activation gene 3"
                     /codon_start=1
                     /product="lymphocyte activation gene 3 protein isoform 1
                     precursor"
                     /protein_id="NP_002277.4"
                     /db_xref="CCDS:CCDS8561.1"
                     /db_xref="GeneID:3902"
                     /db_xref="HGNC:HGNC:6476"
                     /db_xref="MIM:153337"
                     /translation="MWEAQFLGLLFLQPLWVAPVKPLQPGAEVPVVWAQEGAPAQLPC
                     SPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVL
                     SVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSC
                     RLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPH
                     HHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGS
                     RVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCH
                     IHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRS
                     FSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHL
                     LLFLILGVLSLLLLVTGAFGFHLWRRQWRPRRFSALEQGIHPPQAQSKIEELEQEPEP
                     EPEPEPEPEPEPEPEQL"
     exon            392..539
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            540..844
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            845..1114
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1390
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1633
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1764
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1976
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1954..1959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /note="hexamer: AATAAA"
     polyA_site      1976
                     /gene="LAG3"
                     /gene_synonym="CD223"
                     /note="major polyA site"
ORIGIN      
        1 agagaccagc agaacggcat cccagccacg acggccactt tgctctgtct gctctccgcc
       61 acggccctgc tctgttccct gggacacccc cgcccccacc tcctcaggct gcctgatctg
      121 cccagctttc cagctttcct ctggattccg gcctctggtc atccctcccc accctctctc
      181 caaggccctc tcctggtctc ccttcttcta gaaccccttc ctccacctcc ctctctgcag
      241 aacttctcct ttacccccca ccccccacca ctgccccctt tccttttctg acctcctttt
      301 ggagggctca gcgctgccca gaccatagga gagatgtggg aggctcagtt cctgggcttg
      361 ctgtttctgc agccgctttg ggtggctcca gtgaagcctc tccagccagg ggctgaggtc
      421 ccggtggtgt gggcccagga gggggctcct gcccagctcc cctgcagccc cacaatcccc
      481 ctccaggatc tcagccttct gcgaagagca ggggtcactt ggcagcatca gccagacagt
      541 ggcccgcccg ctgccgcccc cggccatccc ctggcccccg gccctcaccc ggcggcgccc
      601 tcctcctggg ggcccaggcc ccgccgctac acggtgctga gcgtgggtcc cggaggcctg
      661 cgcagcggga ggctgcccct gcagccccgc gtccagctgg atgagcgcgg ccggcagcgc
      721 ggggacttct cgctatggct gcgcccagcc cggcgcgcgg acgccggcga gtaccgcgcc
      781 gcggtgcacc tcagggaccg cgccctctcc tgccgcctcc gtctgcgcct gggccaggcc
      841 tcgatgactg ccagcccccc aggatctctc agagcctccg actgggtcat tttgaactgc
      901 tccttcagcc gccctgaccg cccagcctct gtgcattggt tccggaaccg gggccagggc
      961 cgagtccctg tccgggagtc cccccatcac cacttagcgg aaagcttcct cttcctgccc
     1021 caagtcagcc ccatggactc tgggccctgg ggctgcatcc tcacctacag agatggcttc
     1081 aacgtctcca tcatgtataa cctcactgtt ctgggtctgg agcccccaac tcccttgaca
     1141 gtgtacgctg gagcaggttc cagggtgggg ctgccctgcc gcctgcctgc tggtgtgggg
     1201 acccggtctt tcctcactgc caagtggact cctcctgggg gaggccctga cctcctggtg
     1261 actggagaca atggcgactt tacccttcga ctagaggatg tgagccaggc ccaggctggg
     1321 acctacacct gccatatcca tctgcaggaa cagcagctca atgccactgt cacattggca
     1381 atcatcacag tgactcccaa atcctttggg tcacctggat ccctggggaa gctgctttgt
     1441 gaggtgactc cagtatctgg acaagaacgc tttgtgtgga gctctctgga caccccatcc
     1501 cagaggagtt tctcaggacc ttggctggag gcacaggagg cccagctcct ttcccagcct
     1561 tggcaatgcc agctgtacca gggggagagg cttcttggag cagcagtgta cttcacagag
     1621 ctgtctagcc caggtgccca acgctctggg agagccccag gtgccctccc agcaggccac
     1681 ctcctgctgt ttctcatcct tggtgtcctt tctctgctcc ttttggtgac tggagccttt
     1741 ggctttcacc tttggagaag acagtggcga ccaagacgat tttctgcctt agagcaaggg
     1801 attcaccctc cgcaggctca gagcaagata gaggagctgg agcaagaacc ggagccggag
     1861 ccggagccgg aaccggagcc cgagcccgag cccgagccgg agcagctctg acctggagct
     1921 gaggcagcca gcagatctca gcagcccagt ccaaataaac tccctgtcag cagcaa
//
