LOCUS       NM_033277                552 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens lacritin (LACRT), mRNA.
ACCESSION   NM_033277
VERSION     NM_033277.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Georgiev,G.A., Gh,M.S., Romano,J., Dias Teixeira,K.L., Struble,C.,
            Ryan,D.S., Sia,R.K., Kitt,J.P., Harris,J.M., Hsu,K.L., Libby,A.,
            Odrich,M.G., Suarez,T., McKown,R.L. and Laurie,G.W.
  TITLE     Lacritin proteoforms prevent tear film collapse and maintain
            epithelial homeostasis
  JOURNAL   J Biol Chem 296, 100070 (2021)
   PUBMED   33187980
  REMARK    GeneRIF: Lacritin proteoforms prevent tear film collapse and
            maintain epithelial homeostasis.
REFERENCE   2  (bases 1 to 552)
  AUTHORS   Dias-Teixeira,K., Horton,X., McKown,R., Romano,J. and Laurie,G.W.
  TITLE     The Lacritin-Syndecan-1-Heparanase Axis in Dry Eye Disease
  JOURNAL   Adv Exp Med Biol 1221, 747-757 (2020)
   PUBMED   32274735
  REMARK    GeneRIF: The Lacritin-Syndecan-1-Heparanase Axis in Dry Eye Disease
            Review article
REFERENCE   3  (bases 1 to 552)
  AUTHORS   Aass,C., Norheim,I., Eriksen,E.F., Bornick,E.C., Thorsby,P.M. and
            Pepaj,M.
  TITLE     Establishment of a tear protein biomarker panel differentiating
            between Graves' disease with or without orbitopathy
  JOURNAL   PLoS One 12 (4), e0175274 (2017)
   PUBMED   28419103
  REMARK    GeneRIF: Data show increased levels of lysozyme C (LYZ), lacritin
            (LACRT) and zinc-alpha-2 glycoprotein 1 (AZGP1) in pooled tear
            fluid sample from Graves' disease (GD) patients with
            moderate-to-severe Graves' orbitopathy (GO) compared with GD
            patients without clinical signs of GO.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 552)
  AUTHORS   Vantaku,V.R., Gupta,G., Rapalli,K.C. and Karnati,R.
  TITLE     Lacritin Salvages Human Corneal Epithelial Cells from
            Lipopolysaccharide Induced Cell Death
  JOURNAL   Sci Rep 5, 18362 (2015)
   PUBMED   26670139
  REMARK    GeneRIF: cytoprotective effect of lacritin is mediated through
            Cyclooxygenase-2 (COX-2).
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 552)
  AUTHORS   Wang,W., Despanie,J., Shi,P., Edman-Woolcott,M.C., Lin,Y.A.,
            Cui,H., Heur,J.M., Fini,M.E., Hamm-Alvarez,S.F. and MacKay,J.A.
  TITLE     Lacritin-mediated regeneration of the corneal epithelia by protein
            polymer nanoparticles
  JOURNAL   J Mater Chem B 2 (46), 8131-8141 (2014)
   PUBMED   25530855
REFERENCE   6  (bases 1 to 552)
  AUTHORS   Koo,B.S., Lee,D.Y., Ha,H.S., Kim,J.C. and Kim,C.W.
  TITLE     Comparative analysis of the tear protein expression in blepharitis
            patients using two-dimensional electrophoresis
  JOURNAL   J Proteome Res 4 (3), 719-724 (2005)
   PUBMED   15952718
  REMARK    GeneRIF: Lacritin is one of nine tear proteins downregulated in
            patients suffering from blepharitis, or inflammation of the eyelid
            often associated with dry eye.
REFERENCE   7  (bases 1 to 552)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
  REMARK    GeneRIF: Lacritin is the sixth most common transcript in the human
            lacrimal gland based on EST sequencing, and is one of twenty-nine
            'lacrimal preferred' genes.
REFERENCE   8  (bases 1 to 552)
  AUTHORS   Weigelt,B., Bosma,A.J. and van 't Veer,L.J.
  TITLE     Expression of a novel lacrimal gland gene lacritin in human breast
            tissues
  JOURNAL   J Cancer Res Clin Oncol 129 (12), 735-736 (2003)
   PUBMED   14574570
  REMARK    GeneRIF: expressed in human breast tumors, breast cancer cell
            lines, and normal breast
REFERENCE   9  (bases 1 to 552)
  AUTHORS   Kumar,R., Huebner,A. and Laurie,G.W.
  TITLE     Genetic separation of the human lacritin gene ('LACRT') and triple
            A (Allgrove) syndrome on 12q13
  JOURNAL   Adv Exp Med Biol 506 (Pt A), 167-174 (2002)
   PUBMED   12613904
  REMARK    GeneRIF: Lacritin gene maps near but not within triple A syndome
            region. Alacrimia in triple a syndrome not due to mutation in
            lacritin gene.
REFERENCE   10 (bases 1 to 552)
  AUTHORS   Sanghi,S., Kumar,R., Lumsden,A., Dickinson,D., Klepeis,V.,
            Trinkaus-Randall,V., Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     cDNA and genomic cloning of lacritin, a novel secretion enhancing
            factor from the human lacrimal gland
  JOURNAL   J Mol Biol 310 (1), 127-139 (2001)
   PUBMED   11419941
  REMARK    GeneRIF: Tissue microarray of seventy-five different human tissues,
            including mammary gland, detected expression only in lacrimal
            acinar and salivary ductal cells.  Thyroid immunostaining was
            equivocal.  These data agree with Clonetech RNA dot blot analysis.
            GeneRIF: Lacritin is detected in human tears and promotes tear
            secretion in vitro when added to rat lacrimal acinar cells via a
            pathway apparently independent of the M3 receptor.  It is mitogenic
            at low ng/ml levels for salivary ductal cells.
            GeneRIF: Lacritin binds several basment membrane components
            (collagen IV, nidogen, fibronectin, laminin-1).  Also vitronectin,
            but not collagen I or heparin.  Yet it is secreted apically and
            possibly may form non-covalent complexes with tear fibronectin.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079310.20.
            
            On Nov 26, 2018 this sequence version replaced NM_033277.1.
            
            Summary: The protein encoded by this gene is highly expressed in
            the lacrimal glands and localized primarily to secretory granules
            and secretory fluid. It augments lacrimal acinar cell secretion,
            promotes ductal cell proliferation, and stimulates signaling
            through tyrosine phosphorylation and release of calcium. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CD724194.1, BC062217.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMN03465412
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257867.5/ ENSP00000257867.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AC079310.20        72777-72888         c
            113-166             AC079310.20        71173-71226         c
            167-307             AC079310.20        70234-70374         c
            308-409             AC079310.20        69731-69832         c
            410-552             AC079310.20        68804-68946         c
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..552
                     /gene="LACRT"
                     /note="lacritin"
                     /db_xref="GeneID:90070"
                     /db_xref="HGNC:HGNC:16430"
                     /db_xref="MIM:607360"
     exon            1..112
                     /gene="LACRT"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..471
                     /gene="LACRT"
                     /note="extracellular glycoprotein lacritin"
                     /codon_start=1
                     /product="extracellular glycoprotein lacritin precursor"
                     /protein_id="NP_150593.1"
                     /db_xref="CCDS:CCDS8883.1"
                     /db_xref="GeneID:90070"
                     /db_xref="HGNC:HGNC:16430"
                     /db_xref="MIM:607360"
                     /translation="MKFTTLLFLAAVAGALVYAEDASSDSTGADPAQEAGTSKPNEEI
                     SGPAEPASPPETTTTAQETSAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKA
                     GKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA"
     exon            113..166
                     /gene="LACRT"
                     /inference="alignment:Splign:2.1.0"
     exon            167..307
                     /gene="LACRT"
                     /inference="alignment:Splign:2.1.0"
     exon            308..409
                     /gene="LACRT"
                     /inference="alignment:Splign:2.1.0"
     exon            410..552
                     /gene="LACRT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      533..538
                     /regulatory_class="polyA_signal_sequence"
                     /gene="LACRT"
                     /note="hexamer: ATTAAA"
     polyA_site      552
                     /gene="LACRT"
                     /note="major polyA site"
ORIGIN      
        1 attccccaga ccttctgcag attctgtggt tatactcact cctcatccca aagaatgaaa
       61 tttaccactc tcctcttctt ggcagctgta gcaggggccc tggtctatgc tgaagatgcc
      121 tcctctgact cgacgggtgc tgatcctgcc caggaagctg ggacctctaa gcctaatgaa
      181 gagatctcag gtccagcaga accagcttca cccccagaga caaccacaac agcccaggag
      241 acttcggcgg cagcagttca ggggacagcc aaggtcacct caagcaggca ggaactaaac
      301 cccctgaaat ccatagtgga gaaaagtatc ttactaacag aacaagccct tgcaaaagca
      361 ggaaaaggaa tgcacggagg cgtgccaggt ggaaaacaat tcatcgaaaa tggaagtgaa
      421 tttgcacaaa aattactgaa gaaattcagt ctattaaaac catgggcatg agaagctgaa
      481 aagaatggga tcattggact taaagcctta aatacccttg tagcccagag ctattaaaac
      541 gaaagcatcc aa
//
